• Title/Summary/Keyword: static culture

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Persistence and Change in the Black Forest Ethnic Dress Tradition

  • Hughes, Amy S.;Torntore, Susan J.;Ogle, Jennifer Paff
    • International Journal of Costume and Fashion
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    • v.15 no.1
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    • pp.1-18
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    • 2015
  • The ethnic dress of Germany's Black Forest, called Tracht, dates back to the 16th century. Although most people living within the Black Forest do not presently wear Trachten, some persistence in this tradition exists. This study explored the factors that have supported the persistence of the Trachten tradition, specifically related to the wearing and crafting of Trachten by women, in a contemporary society. A qualitative, ethnographic approach was adopted. Data were collected via observations and interviews in the Black Forest. Thematic analyses revealed that the maintenance of the Trachten tradition was linked to varied factors that revolved around the overarching themes of both persistence and change. Interpretations were supported by theory proposing that ethnic dress is not static, but rather, changes across space and time in ways that enable its persistence. The persistence of the Trachten tradition was linked to formalized practice, meaningful identities, and desires to preserve and promote local culture. Additionally, the persistence of the Trachten tradition was fostered by change in the tradition, including the conceptualization of Trachten as a "lived practice" and the negotiation of Trachten authenticity.

A Comparative Static Study on the Variables' Significance to Urban Quality of Life (도시인의 '삶의 질' 결정요인에 관한 비교정태분석)

  • Kim, Dong-Yoon
    • Journal of The Korean Digital Architecture Interior Association
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    • v.13 no.3
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    • pp.15-23
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    • 2013
  • As a succeeding analysis on the variables' significance to 'Quality of Life(QOL)'(Kim, Dong-Yoon, 2012a), which has a purpose of trying to enhance the intersubjectiveness by means of quantitive methods to find the factors on the QOL, this study aims at finding the time serial change in the deciding factors to deepen the related researches on the theme. Using the similar statistical methods such as multiple regression and factor analysis for "2012 Seoul Survey" this study shows two similar points and one differentia. The similarities include the fact that five subcategories of happiness have relative statistical importance in the order of (1)financial condition, (2)health condition, (3)social activities, (4)community relationship and (5)family life and the fact that the five subcategories are conceptually different from the 5 deciding factors, which means the possibility to improve the survey design. And the difference is the 5 factors such as (1) social trust - amenities, (2)cultural life, (3)social indiscrimination - hope for elevation, (4)sharing - giving and (5)community spirit which are largely different from preceding study. Although only the first two factors have statistical significance this result has a implication that they reflect the new paradigm of social capital and the essential necessity of human survival; sharing-community-culture.

Characteristics and Model for Growth of Rhizopus oryzae on the Simulated Gas-solid Interface

  • Jia, Shiru;Kong, Rixiang;Dong, Huijun;Kwun, Kyu-Hyuk;Kim, Sun-Il;Cho, Ki-An;Choi, Du Bok
    • Korean Journal of Environmental Biology
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    • v.22 no.4
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    • pp.494-500
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    • 2004
  • In order to investigate the effect on morphology of Rhizopus oryzae and production of lactic acid, various interface materials were used. Morphology of fungal showed sheet and flock when resin was added. The production of lactic acid was increased dramatically when interface materials were added. Furthermore, the effect of resin was more significant than that of others. It was assumed that interface materials could absorb substrate and microorganism together, so microorganism was not inhibited by substrate. The effect of static electric field on the interface culture was studied. When the exerting potential was 6.78 voltage, the biomass y was obviously higher than that of zero voltage. A simulated gas-solid interface system was developed to study the growth and two phases model for the growth of Rhizopus oryzae was build up that depended on the symmetric branching theory. An important parameter F was researched. The results indicated that the value of F had obvious difference at exponential and deceleration period, respectively.

Cell Culture Microbioassay for the Water Pollution Monitoring (세포배양 생화학적 기법에 의한 수환경오염 평가)

  • 오승민;정규혁
    • Toxicological Research
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    • v.16 no.4
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    • pp.285-291
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    • 2000
  • So far, investigation of environmental pollution has been achieved in field study. This remains the most exhaustive approach, current dimensions of environmental researches and their inherent complexity require that relatively inexpensive and simple laboratory procedures are developed to make possible the screening of large numbers of sites and samples. At this point. microbioassay has been high-lighted. The purpose of this study is to evaluate the water pollution using microbioassay. Two microbioassay methods were optimized and validated for the sensitive and quantitative determination of total toxic effects in the water. EROD(Ethoxyresorufin-O-deethylase) microbioassay was focused to detect PARs, PCBs and dioxinlike components in the water and E-screen assay to xenoestrogens. The EROD microbioassay was executed in rat hepatoma cell line, H4IIE and E-screen assay in MCF7-BUS cell line. Kumho river was selected for this study. 5ι of river water was extracted using combined solid-phase extraction in static adsorption mode with soxhlet extraction. Pollutants adsorbed to the XAD-4 resin were recovered by elution with ethyl acetate and methylene chloride (1 : 9). Toxic effects of extracts were determined by EROD-microbioassay and E-screen assay. EROD activities of water samples were 7.24-72.24 ng/ι MEQ. The estrogenic effect of various water samples is quantitatively evaluated by EEQ. The EEQ of samples range from 0.05 to 6.07 ng-EEQ/ι. These results suggested that Kumho river was highly polluted with organic toxic chemicals.

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The Effect of a Long-Term Cyclic Strain on Human Dermal Fibroblasts Cultured in a Bioreactor on Chitosan-Based Scaffolds for the Development of Tissue Engineered Artificial Dermis

  • Lim, Sae-Hwan;Son, Young-Sook;Kim, Chun-Ho;Shin, Heung-Soo;Kim, Jong-Il
    • Macromolecular Research
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    • v.15 no.4
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    • pp.370-378
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    • 2007
  • Mechanical stimulation is known to activate several cellular signal transduction pathways, leading to the induction of signaling molecules and extracellular matrix (ECM) proteins, thereby modulating cellular activities, such as proliferation and survival. In this study, primary human dermal fibroblasts (HDFs) were seeded onto chitosan-based scaffolds, and then cultured for 3 weeks in a bioreactor under a cyclic strain of 1 Hz frequency. Compared to control samples cultured under static conditions, the application of a cyclic strain stimulated the proliferation of HDFs in I week, and by week 3 the thickness of the cell/scaffold composites increased 1.56 fold. Moreover, immunohistochemical staining of the culture media obtained from the cell/scaffold samples subjected to the cyclic strain, revealed increases in the expression and secretion of ECM proteins, such as fibronectin and collagen. These results suggest that the preconditioning of cell/scaffold composites with a cyclic strain may enhance the proliferation of HDFs, and even facilitate integration of the engineered artificial dermal tissue into the host graft site.

THE FINITE ELEMENTS ANALYSIS IN THE THREE DIMENSIONAL CELL CULTURE MODEL OF THE COLLAGEN MATRIX ACCORDING TO THE APPLICATION FORCE (교원섬유 기질을 이용한 3차원 세포 배양 모델에 기계적인 힘을 가하는 경우 기질을 통하여 세포에 가해지는 힘의 특성에 대한 비교 분석 연구)

  • Kim, Hyeon-Jong;Rhyu, In-Chul;Park, Jun-Woo;Kim, Seong-Gon
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.34 no.2
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    • pp.151-156
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    • 2008
  • Different kinds of forces can be applied to the biological tissue. The analysis of the applied force is highly important to explain the mechanism of cellular response. In this study, the applied force to the collagen gel was analyzed by the finite elements analysis. The model received two different kinds of static force (compression and tension). The force range was 50g to 400g. In results, von Mises stress was concentrated in the peripheral region in the compression model. It was concentrated in the central area in the tension model. However, the compressive force was high in the peripheral area of the compression model and the tensional force was also high in the same area of the tension model. In conclusion, the applied force could be different to the region and it should be considered in the experiment to analyze the effects of the mechanical force on the cells.

Effect of Lactate and Corn Steep Liquor on the Production of Bacterial Cellulose by Gluconacetobacter persimmonis $KJ145^T$

  • Jang, Se-Young;Jeong, Yong-Jin
    • Food Science and Biotechnology
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    • v.14 no.5
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    • pp.561-565
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    • 2005
  • In this study, we attempted to assess the effects of lactate and com steep liquor (CSL) on the production of bacterial cellulose (BC) by Gluconacetobacter persimmonis $KJ145^T$. The optimal condition for the production of BC was a lactate concentration of 1% (w/v) and a CSL concentration of 10% (w/v). Under these optimal conditions, 6 days of fermentation produced 6.90 g/L of BC. Both the BC production yield and cell growth increased continuously until the 20th day of fermentation, by which time 17.0 g/L had been produced. In a static culture trial, in which plastic containers were used as fermentation chambers for 6 days of fermentation, the BC production yield in the group initially cultured with 500 mL medium was higher than that of the 750 and 1000 mL media. In addition, the texture of the BC was examined according to its post-treatment in order to determine conditions for optimal textural characteristics. The strength, hardness, and other characteristics of the BC were negatively correlated with sucrose concentration, but were largely positively correlated with NaCl concentration. With regards to the effect of pH on textural change, BC strength and hardness were elevated at pH 2 and 8 but reduced at pH 4 and 6, indicating that the texture of the BC is extremely sensitive to treatment conditions.

Production of Bacterial Cellulose and Its Modification (박테리아 셀룰로오스의 생산 및 개질)

  • 민두식;조남석;최태호
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.29 no.3
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    • pp.26-33
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    • 1997
  • The bacterial celluloses are very different in its physical, chemical and morphological structures compared to wood cellulose. These fibers have many unique properties that are potentially and commercially beneficial. This study was aimed to elucidate the production of bacterial celluloses and to improve their physical properties by chemical pretreatment. Bacterial celluloses produced by static culture had gel-like pellicle structure. The pellicle thickness was increased with the increasing time, and its layer was about 1.8cm after one-month incubation. The pellicles extruded from the cells of Acetobacter had a non-crystalline structure during initial growing stages, gradually getting crystaliyzed with the incubation time elapse, and eventually fumed to the cellulose I crystals. Young's modulus of bacterial cellulose sheet was increased with increasing NaOH concentration, and resulted in the highest at 5% NaOH concentration. Similar results with NaClO3 pretreatment can be observed. Too concentrated alkali solutions induced the destruction of cellulose fibrils and changed the mechanical properties of the sheets. These alkaline pretreatment have removed non-cellulosic components(NCC) from the bacterial cellulose, and enhanced inter-abrillar bonding by direct close contact among cellulosic fibrils.

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Seismic fragility assessment of shored mechanically stabilized earth walls

  • Sheida Ilbagitaher;Hamid Alielahi
    • Geomechanics and Engineering
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    • v.36 no.3
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    • pp.277-293
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    • 2024
  • Shored Mechanically Stabilized Earth (SMSE) walls are types of soil retaining structures that increase soil stability under static and dynamic loads. The damage caused by an earthquake can be determined by evaluating the probabilistic seismic response of SMSE walls. This study aimed to assess the seismic performance of SMSE walls and provide fragility curves for evaluating failure levels. The generated fragility curves can help to improve the seismic performance of these walls through assessing and controlling variables like backfill surface settlement, lateral deformation of facing, and permanent relocation of the wall. A parametric study was performed based on a non-linear elastoplastic constitutive model known as the hardening soil model with small-strain stiffness, HSsmall. The analyses were conducted using PLAXIS 2D, a Finite Element Method (FEM) program, under plane-strain conditions to study the effect of the number of geogrid layers and the axial stiffness of geogrids on the performance of SMSE walls. In this study, three areas of damage (minor, moderate, and severe) were observed and, in all cases, the wall has not completely entered the stage of destruction. For the base model (Model A), at the highest ground acceleration coefficient (1 g), in the moderate damage state, the fragility probability was 76%. These values were 62%, and 54%, respectively, by increasing the number of geogrids (Model B) and increasing the geogrid stiffness (Model C). Meanwhile, the fragility values were 99%, 98%, and 97%, respectively in the case of minor damage. Notably, the probability of complete destruction was zero percent in all models.

Probiotic Potential of Plant-Derived Lactic Acid Bacteria with Antihypertensive Activity (항고혈압 활성을 가진 식물유래 젖산균의 생균제 특성)

  • Lee, Ye-Ram;Son, Young-Jun;Park, Soo-Yun;Jang, Eun-Young;Yoo, Ji-Yeon;Son, Hong-Joo
    • Journal of Environmental Science International
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    • v.25 no.6
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    • pp.789-798
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    • 2016
  • Lactic acid bacteria (LAB) are industrially important microorganisms for probiotics. The recent widespread application of LAB for preparation of functional food is attributable to the accumulating scientific evidence showing their beneficial effects on human health. In this study, we isolated and characterized plant-derived LAB that show angiotensin-converting enzyme (ACE) inhibitory and antioxidant activities. The selected strain K2 was isolated from Kimchi, and identified as Lactobacillus plantarum by 16S rRNA gene analysis. The strain grew under static and shaking culture systems. They were also able to grow in different culture conditions like $25^{\circ}C{\sim}37^{\circ}C$ temperature, 4~10 pH range and ~6% NaCl concentration. L. plantarum K2 was highly resistant to acid stress; survival rate of the strain at pH 2.5 and 3 were 80% and 91.6%, respectively. The strain K2 also showed high bile resistance to 0.3% bile bovine and 0.3% bile extract with more than 74% of survival rate. The cell grown on MRS agar plate containing bile extract formed opaque precipitate zones around the colonies, indicating they have bile salt hydrolase activity. The strain showed an inhibitory activity against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Listeria monocytogenes; antibacterial activity was probably due to the lactic acid. The K2 strain showed relatively higher autoaggregation values, antihypertensive and antioxidant activities. These results suggest that L. plantarum K2 could be not only applied as a pharmabiotic for human health but also is also starter culture applicable to fermentative products.