Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
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The Effect of a Long-Term Cyclic Strain on Human Dermal Fibroblasts Cultured in a Bioreactor on Chitosan-Based Scaffolds for the Development of Tissue Engineered Artificial Dermis

  • Lim, Sae-Hwan (Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences) ;
  • Son, Young-Sook (Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences) ;
  • Kim, Chun-Ho (Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences) ;
  • Shin, Heung-Soo (Department of Bioengineering, Hanyang University) ;
  • Kim, Jong-Il (Colledge of Natural Sciences, Seoul Women's University)
  • Published : 2007.06.30
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Abstract

Mechanical stimulation is known to activate several cellular signal transduction pathways, leading to the induction of signaling molecules and extracellular matrix (ECM) proteins, thereby modulating cellular activities, such as proliferation and survival. In this study, primary human dermal fibroblasts (HDFs) were seeded onto chitosan-based scaffolds, and then cultured for 3 weeks in a bioreactor under a cyclic strain of 1 Hz frequency. Compared to control samples cultured under static conditions, the application of a cyclic strain stimulated the proliferation of HDFs in I week, and by week 3 the thickness of the cell/scaffold composites increased 1.56 fold. Moreover, immunohistochemical staining of the culture media obtained from the cell/scaffold samples subjected to the cyclic strain, revealed increases in the expression and secretion of ECM proteins, such as fibronectin and collagen. These results suggest that the preconditioning of cell/scaffold composites with a cyclic strain may enhance the proliferation of HDFs, and even facilitate integration of the engineered artificial dermal tissue into the host graft site.

Keywords

References

  1. D. Williams, ESB Satellite Consensus Conference, Sorrento, 2005, p 9
  2. B. S. Kim and D. J. Mooney, J. Biomech. Eng., 122, 210 (2000) https://doi.org/10.1115/1.429651
  3. O. Akhouayri, M. H. Lafage-Proust, A. Rattner, N. Laroche, A. Caillot-Augusseau, C. Alexandre, and L. Vico, J. Cell. Biochem., 76, 217 (1999)
  4. B. S. Kim, J. Nikolovski, J. Bonadio, and D. J. Mooney, Nature Biotech., 17, 979 (1999)
  5. K.T. Nguyen, S. R. Frye, S. G. Eskin, C. Patterson, M. S. Runge, and L. V. McIntire, Hypertension, 38, 1038 (2001) https://doi.org/10.1161/01.HYP.38.1.1
  6. E. K. Lee, J. S. Lee, H. S. Park, C. H. Kim, Y. J. Gin, and Y. Son, Tissue Eng. and Regen. Med., 2, 29 (2005)
  7. H. S. Park, Y. Y. Kim, C. H. Kim, and Y. Son, Tissue Eng., submit (2006)
  8. A. Lahiji, A. Sohrabi, D. S. Hungerford, and C. G. Frondoza, J. Biomed. Mater. Res., 51, 586 (2000)
  9. A. Eser Elcin, Y. M. Elcin, and G. D. Pappas, Neurol. Res., 20, 648 (1998)
  10. K. M. Varum, M. M. Myhr, R. J. Hjerde, and O. Smidsrod, Carbohydr. Res., 299, 99 (1997)
  11. M. N. Taravel and A. Domard, Biomaterials, 17, 451 (1996)
  12. A. Denuziere, D. Ferrier, O. Damour, and A. Domard, Biomaterials, 19, 1275 (1998)
  13. C. Chatelet, O. Damour, and A. Domald, Biomaterials, 22, 262 (2001) https://doi.org/10.1016/S0142-9612(00)00074-0
  14. C. H. Kim, S.-H. Lim, Y. J. Choi, K.-S. Park, H. S. Park, Y. J. Gin, C. W. Park, and Y. Son, Macromol. Res., 12, 367 (2004)
  15. R. G. Zhan and P. X. Ma, J Biomed. Mater. Res., 44, 446 (1999)
  16. H. S. Park, H. J. Kang, C. H. Kim, C. H. Kim, E. S. Han, K. B. Han, T. H. Kim, Y. J. Gin, and Y. S. Son, Tissue Eng., 10, 343 (2004)
  17. H. Eshel and Y. Lanir, Annals Biomed. Eng., 29, 164 (2001)
  18. J. M. Grunkemeier, W. B. Tsai, C. D. McFarland, and T. A. Horbett, Biomaterials, 21, 2243 (2000)
  19. D. J. Fernandes, J. V. Bonacci, and A. G. Stewart, Curr. Drug Targets, 7, 567 (2006) https://doi.org/10.2174/138945006776818700
  20. Y. Mao and J. E. Schwarzbauer, Matrix Biol., 24, 389 (2005) https://doi.org/10.1016/j.matbio.2005.06.008
  21. N. D. Gallant, K. E. Michael, and A. J. Garcia, Mol. Biol. Cell., 16, 4329 (2005)
  22. R. Hynes, Cell, 110, 673 (2002)
  23. A. J. García and D. Boettiger, Biomaterials, 20, 2427 (1999)
  24. M. Chiquet, Matrix Biol., 18, 417 (1999) https://doi.org/10.1016/S0945-053X(99)00039-6
  25. H. Inoh, N. Ishiguro, S. Sawazaki, H. Amma, M. Miyazu, H. Iwata, M. Sokabe, and K. Naruse, FASEB J., 16, 405 (2002) https://doi.org/10.1096/fj.01-0300rev