• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.274-279
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• 2012

For the quantitative analysis of genetically modified (GM) maize in processed foods, primer sets and probes based on the 35S promoter (p35S), nopaline synthase terminator (tNOS), p35S-hsp70 intron, and zSSIIb gene encoding starch synthase II for intrinsic control were designed. Polymerase chain reaction (PCR) products (80~101 bp) were specifically amplified and the primer sets targeting the smaller regions (80 or 81 bp) were more sensitive than those targeting the larger regions (94 or 101 bp). Particularly, the primer set 35F1-R1 for p35S targeting 81 bp of sequence was even more sensitive than that targeting 101 bp of sequence by a 3-log scale. The target DNA fragments were also specifically amplified from all GM labeled food samples except for one item we tested when 35F1-R1 primer set was applied. A reference plasmid pGMmaize (3 kb) including the smaller PCR products for p35S, tNOS, p35S-hsp70 intron, and the zSSIIb gene was constructed for real-time PCR (RT-PCR). The linearity of standard curves was confirmed by using diluents ranging from $2{\times}10^1{\sim}10^5$ copies of pGMmaize and the $R^2$ values ranged from 0.999~1.000. In the RT-PCR, the detection limit using the novel primer/probe sets was 5 pg of genomic DNA from MON810 line indicating that the primer sets targeting the smaller regions (80 or 81 bp) could be used for highly sensitive detection of foreign DNA fragments from GM maize in processed foods.

• 한국미생물·생명공학회지
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• 제44권1호
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• pp.98-105
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• 2016

강원도를 비롯한 전국에서 발효식품 44점으로부터 유해균에 대한 생육억제 활성이 우수한 고초균주를 분리 및 동정하였으며, 생육억제 활성이 가장 우수하였던 Bacillus amyloliquefaciens MBE1283로 명명된 균주의 유해균에 대한 생육억제 활성, 전분분해 효소활성 및 생육특성을 조사하였다. Escherichia coli O157:H7 KCCM40406을 비롯한 8종류의 유해균에 대한 생육억제 활성을 평가한 결과, 막걸리에서 분리된 B. amyloliquefaciens MBE1283 균주는 다른 B. amyloliquefaciens 균주들에 비해 유의적으로 높은 유해균 생육억제 활성을 나타내었다. B. amyloliquefaciens MBE1283 균주의 ${\alpha}$-amylase 효소활성은 대조구 균주들과 비교하였을 때 현저히 낮았으나 glucoamylase 효소활성은 대조구로 사용한 균주와 유사한 수준이었다. B. amyloliquefaciens MBE1283 균주의 최적 생육온도는 $45^{\circ}C$로서 대조구 균주보다 온도에 대한 내성이 있는 것으로 판단되었으며, 균체 성장에 적합한 최적 배지는 pH 6이었다. 본 연구를 통하여 막걸리로부터 분리된 B. amyloliquefaciens MBE1283 균주는 여러 종류의 유해균에 항균활성을 지니며 높은 온도와 낮은 pH에 대하여 내성을 지닌 것을 알 수 있었다.

• 한국식품과학회지
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• 제25권5호
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• pp.411-416
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• 1993

이 연구에서는 새로운 방법으로 제조된 효모 자가분해물이 쇠고기 향신료로서 이용이 가능한지 조사하기 위하여 먼저, 효모 자가분해물을 사용하여 쇠고기 맛 국물을 제조하기 위한 재료들의 최적 수준을 결정한 다음, 이를 기초로 하여 된장찌개 믹스에 사용되는 재료들의 최적 수준을 결정하였다. 최적 수준은 반응표면 방법을 사용하여 결정되었다. 쇠고기 맛 국물의 최적수준은 효모자가분해물을 8g/l, 핵산조미료는 0.8g/l, 그리고 호박산은 0.09g/l이었으며, 된장찌개 스프 믹스의 최적 수준은 쇠고기 맛 국물 재료는 8g/l, 된장분말은 28g/l, 그리고 옥수수전분은 16g/1이었다. 효모 자가분해물을 사용한 된장찌개와 소고기 분말이 첨가된 기존 제품을 사용한 된장찌개의 기호도를 비교한 결과 유의적인 차이가 없었다. 이 연구 결과 새로운 방법으로 제조된 효모 자가분해물이 된장찌개와 같은 제품에서 쇠고기 향신료로 이용될 수 있다고 본다.

• 한국식생활문화학회지
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• 제16권4호
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• pp.269-275
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• 2001

The present research was designed to collect recipe of set up a standard recipe and analyze the nutrients of broiled black goat meat (a kind of native local foods in Busan and south province). The results of the study are summarized as follows. The recipe for goat(lamb) meat was recorded in Sanlimkyounggae and Jungbosanlimkyounggae between 1715 and 1827. The goat meat was explained as food for health, rejuvenation and recuperation Since late 1800, it has been utilized for such use. The flavor of broiled black goat meat is influenced by seasonings, fruit juice, heating treatment and cooker. And it also could be affected by the method of slaughter and the sex of the goat. There are lot of element in seasoning. Red pepper paste, garlic and ginger holdback the bad smell and make the taste better. Onion help to retain the water and improve the flavor. Starch syrup, sugar and sesame oil make the meat smoother, glossier and more tasty. The meat is usually roasted on grill over charcoal. It helps to remove or suppress the bad smell and make the taste better. Softness and scent of the meat depend largely on the way to butcher. Female goat meat tastes smoother and smells better. Fruit juice also improve the softness of the meat. Energy per 100g of the 'broiled black goat meat' is 170kcal. There are protein (23g), fat(2.4g), Ca(159mg), Fe(1.1mg) and so on. Amino acid is mainly comprised of essential amino acid such as cystine, lysine, leucine, and arginine. Fatty acid consists primarily of unsaturated fatty acid like oleic acid, linoleic acid.

• 한국식품과학회지
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• 제21권6호
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• pp.826-831
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• 1989

농후 고추장-물 현탁액의 리올로지 거동을 검토하였다. 리올로지 거동은 Herschel-Bulkley식에 적합하였다. 가수량 12.5-100%의 모든 고추장 현탁액은 전단속도 $0.25-179.20sec^{-1}$에서 항복응력을 갖는 의가소성을 보였으며, 전단속도 $5.16sec^{-1}$ 이상에서는 전단속도가 증가함에 따라 의가소성이 더욱 증가함을 보였다. 또, 이들 의가소성은 가수량의 증가 및 측정온도의 감소로 더욱 약해졌다. 고추장 현탁액의 점조도지수에 대한 온도의존성은 매우 낮았으며 점조도지수 값은 고형분함량의 증가로 낮아졌으나 가열로 증가되었다. 고온에서의 리올로지 거동에 대한 주요인 성분은 발효 중의 미분해 전분 또는 단백질인 것으로 추정되었으며, 식초첨가에 의한 리올로지 거동의 변화는 단지 희석효과에 기인하였다.

• 대한지역사회영양학회지
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• 제9권3호
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• pp.315-325
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• 2004

This study was conducted to develop the standard breakfast menu for those weak groups having insufficient breakfast intake. The following three target groups are classified as: 16-19 years old high school male student, 20-29 years old female who have job or college students, 20-29 years old male or female who have job (double income family). While developing menus for each target groups, we applied several basic guidelines for meal planning as follows: Nutrient intake level was set to $\graction one-Third$ of RDA, while the energy level to $\fraction one-quarter $ of RDAs. Most Sequent meal pattern of Koreans was adapted; Suitabilities of appropriate serving size and cost for middle-income families were considered; Domestic foods and ingredients were used. We developed 24 menus summed by 2 menus for each season and three target groups. When evaluating the menus, most of the breakfast menus were sufficient of nutrients as a meal for the subjects. Three food groups such as grain/starch group, meat/fish/egg/bean group, vegetable/fruit group were included in all menus. Even though milk/dairy products group was not excluded for some menus, other calcium substitutes like anchovies were used. Oil/nut/sugar group was used to a minimum. The average number of foods for each menu was 12.8, which ranged from 10 to 17 depending on the menus. The average weight of the menus including soup was 822 g, 633 g and 730 g for each target group, respectively. The average price of the menu ranged from 2,000 to 3,500 won per person. The above results could be applied at home as well as foodservice institutes and furthermore could offer information for developing breakfast-substituting food products.

• 한국식품위생안전성학회지
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• 제25권1호
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• pp.43-48
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• 2010

침출차와 소스류, 전분류의 각 품목에 대하여 PSL과 TL의 적용 가능성을 검토하기 위하여 각 품목별로 다른 선량으로 방사선 조사를 실시하여 PSL과 TL 분석을 하였다. 침출차 2종은 0, 2, 7kGy, 소스류 2종은 0, 5, 7, 10 kGy, 전분류 3종은 0, 2, 5 kGy로 조사를 실시하였다. PSL의 분석 결과, 침출차는 비조사구에서는 threshold value $T_1$(700) 이하의 낮은 값을 나타내었고 2kGy 이상의 조사구에서는 $T_2$(5000) 이상의 높은 값을 나타내어 방사선 조사 여부 확인이 가능하였다. 이에 비해 소스류는 비조사구에서는 $T_1$ 이하의 값이 나타났지만, 5kGy 이상의 조사구에서 1173, 1773의 $T_1$보다 높고 $T_2$보다 낮은 photon count를 나타내어 중간시료로 나타났다. 또한 전분류에서는 비조사구에서 $T_1$보다 높고 $T_2$보다 낮은 중간값을 나타내어 TL 확인 시험을 필요로 하였다. TL 분석에서는 3가지 품목 모두 비조사구에서는 $300^{\circ}C$ 부근에서 최고의 peak를 가지는 glow curve를 나타내거나 특정적인 TL glow curve를 나타내지 않았다. 그리고 조사구에서는 $150{\sim}250^{\circ}C$ 부근에서 비조사구에서 보이지 않았던 특유의 glow curve를 나타내었고, 조사선량에 따른 intensity의 차이도 보이고 있었다 한편 TL 결과를 검증하기 위하여 재조사에 의한 TL ratio를 산출한 결과 비조사구에서는 0.08 이하를 나타내었으나, 각 선량별 조사구에서는 전분류의 경우 0.07 이상의 낮은 값을 나타내기도 하였으나 TL온도범 위와 glow curve의 형태에 따라서 방사선 조사된 것을 확인할 수 있었다. 따라서 PSL과 TL 분석법의 적용 가능성이 확인되었다.

• 대한지역사회영양학회지
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• 제12권2호
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• pp.189-197
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• 2007

Low glycemic index (GI) foods have been reported to be very important in the control of blood glucose levels in diseases such as diabetes mellitus and obesity, which are becoming more prevalent in Korea. Bread consumption at breakfast and as a snack is also on the rise. To provide low GI alternatives, breads supplemented with dietary fibers-fructooligosaccharide (FOS), ground whole rye, and 2 types of resistance starch (RS2 and RS4)-were developed. The GIs for these breads were evaluated with 13 healthy college students (M6, F7) and sensory tests were done. Four kinds of breads were made through the modification of a basic recipe for white bread (the control, B) as follows: for Bf, the entire amount of sugar was replaced with FOS. For Ryef, 50% of the white wheat flower was replaced with roughly ground wholerye (20 mesh: 12-20 mesh = 1 : 3) in addition to replacing sugar with FOS. For RS2f and RS4f, 20% of the white wheat flower was replaced with RS2 or RS4, in addition to replacing sugar with FOS. The overall preference score of Bf was similar to that of B, while those of Ryef, RS2f and RS4f were lower than that of B, but showed the acceptable degree of the overall preference. The glycemic indices of Ryef and RS2f were 46.1 and 45.9 respectively, which were significantly lower than the GI of B, 67.8. The GIs of Bf and RS4f were, however, 66.7 and 80.5 respectively, showing no significant difference compared to B. The glycemic loads for a 30 g serving were 9.5, 5.9, 6.2, 11.0 and 9.0 for B, Bf, Ryef, RS2f and RS4f, respectively. In conclusion, addition of RS2 or roughly ground whole rye to the dough formula significantly lowered the GI. Since the preferences shown for those two breads were acceptable, they may be recommended as a substitute for white bread fir persons who need blood glucose management. More studies on the bread making process are required to improve preference and acceptance. Although GI lowering effects for F and RS4 were not found in this study, further studies are needed to verify their effects.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.700-703
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• 2001

The influence of process conditions on highly branched glucooligosaccharides production by mixed culture of Leuconostoc mesenteroides ATCC 13146 and Lipomyces starkeyi ATCC 74054 was studied. We divided the batch culture fermentations into two groups according to inoculation method. One-point inoculation was performed by coinoculation of L. mesenteroides and L. starkeyi at the ration of 10 to 1, and two-point inoculation by L. mesenteroides inoculation first and L. starkeyi inoculation after L. mesenteroides grew to the end of the log phase of growth. Two-point inoculation improved the yield of oligosaccharide by 1.5 to 20 fold more than one-point inoculation. In this process, the highest yield of oligosaccharides (48% of theoretical yield) and productivity (0.85 g/l/h) were obtained with starch as an initial substrate for L. starkeyi growth. The estimated composition of the end product consisted of 31.5% oligosaccharides, 17.6% dextran, and 46.5% mannitol.

• 한국식품과학회지
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• 제20권6호
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• pp.808-811
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• 1988

국내에서 소비되는 산채류에 대하여 thiamine분해능을 thiochrome 형광법과 Lactobacillus viridescens를 이용한 미생물법으로 측정하였고 페놀 화합물의 함량 및 갈색도와의 관계를 규명하였다. 더덕, 도라지, 토란대, 호박꼬지, 표고버섯, 도토리 전분 등은 thiamine분해능이 크지 않았으나 가지, 고구마 줄기, 고사리, 고춧잎, 취, 즉 등은 상당량의 thiamine분해능을 가지고 있었다. Thiamine분해능이 큰 산채류는 페놀 화합물의 함량과 갈색도도 높았다. 따라서 산채류를 식용으로 할 때는 thiamine급원식품과 함께 조리하지 말 것이며 조리 즉시 섭취하는 것이 바람직하다.