• 한국연초학회지
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• 제20권2호
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• pp.231-237
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• 1998

This study was carried out to investigate the effects of curing methods on the concentration of ammonia during curing in burley tobacco leaves. The air-cured tobacco(KB108; Nicotiana tabacum L.) was grown at Chonju Tobacco Experiment Station in 1998 and the tenth leaves from the top on the stalk were harvested. Half of the harvested leaves were cured in normal air curing facility and the other leaves were cured in excessive curing facility. Stalk cut tobaccos were cured in horizontal curing facility. The leaves were sampled every five days from harvesting time to the end of curing(25 days). Ammonia concentration of leaves increased during curing period with a remarkable increase at yellowing stage. The concentration of ammonia was high in the primed cured leaves, while that of the excessive cured leaves was low. It is considered that the lower increase of ammonia in stalk cured leaves may be caused by the translocation from the leaves to the stalk during curing, while that of excessive cured leaves may be caused by the poor decomposition of protein and amino acid during curing by excessive moisture loss and high temperature condition.

• Applied Microscopy
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• 제46권2호
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• pp.83-88
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• 2016

Structural differentiation of the connective stalk in giant duckweed, Spirodela polyrhiza, was examined to reveal the anatomical and ultrastructural characteristics within reduced shoot. The study focuses primarily on structural features of the connective stalk (CT), which connect offspring to their mother fronds. Photoautotrophic offspring fronds remained connected by stalks to mother fronds in the reproductive pockets until separation. The CT originated from the meristematic region of the abaxial frond and joined the fronds laterally with two abscission layers. The most notable features of the CT were polymorphic mitochondria, random occurrences of fibrillar structures in intercellular spaces, and great variability in cell wall thickness. Vascular tissues in CTs were highly reduced, demonstrating only a central vascular strand. Grana with 2 to 4 thylakoids and starch grains were found in the chloroplasts. A chlorophyll assay indicated high chlorophyll concentrations in daughter fronds and low concentrations in CTs. The frond and CT, while physically connected to each other, functioned independently. Despite great reduction in S. polyrhiza, the CT has proven to be very efficient for separating offspring from the mother frond, which lends to its capacity for rapid vegetative reproduction. The ultrastructural aspects of CTs in S. polyrhiza were characterized for the first time in this study.

• 한국연초학회지
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• 제24권2호
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• pp.75-81
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• 2002

This study was performed to investigate the influence of curing conditions on the composition of essential oil during curing process of burley tobacco leaves. The curing conditions were the primed curing in vinyl house (house-curing), air-curing barn (air-curing) and stalk-curing in conventional curing house (stalk-curing). Total 90 compounds are identified from the steam volatile oils of harvest and cured tobacco leaves by GC and GC-MS, respectively. The major components were neophytadiene, hexadecanoic acid, 3,8,13-duvatriene-1,5-diols, oxide-9-methylene-3,13-duvadienols, solanone, megastigma-4,6,8-trien-3-ones, phenylacetaldehyde, $\beta$-phenylethyl alcohol, indole, dihydroactinidiolide and phytol. The amount of alcoholic compounds was decreased more than approximately 50% in cured leaves without regard to the curing conditions. $\beta$-Phenylethyl alcohol and 3,8,13-duvatriene-1,5-diols were decreased more in air curing and stalk curing than in house curing. The amounts of phenylacetaldehyde, solanone, $\beta$-damascone, $\beta$ -damascenone, oxysolanone and megastigma-4,6,8-trien-3-ones as ketonic compounds, dihydroactinidiolide and indole as miscellaneous compounds in air-cured and stalk-cured tobacco leaves were 2 times higher than those in house-cured leaves, while esteric and acidic compounds were not changed largely in content by curing conditions.

• Journal of Biosystems Engineering
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• 제20권1호
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• pp.13-21
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• 1995

This study was conducted to investigate the cutting mechanism of reciprocating knife of combine harvester. The cutting operation of reciprocating knife with the arrangement of the low-cutting and the double-cutting was demonstrated through cutting pattern diagram which was drawn by computer graphics. Various kinds and dimensions of reciprocating knives were analyzed using the developed program. The results are summarized as follows (1) The low-cutting type reciprocating knife was represented similar cutting characteristics to the standard type, but the maximum stalk-deflection was decreased as 1/2 level of the standard type. And the first ledger plate should be designed shorter than the second ledger plate. (2) The bunching area and the maximum stalk-deflection for the double cutting knife almost were not changed since cutting velocity ratio of 0.6, but the secondary cut were occurred at ratio of 0.8 and increased rapidly over these ratio. (3) The double cutting knife was recommended for the high speed combine, because its bunching area and the maximum stalk-deflection were decreased as 1/2 level of the standard type. (4) In order to maintain the proper cutting mechanism characterized by the bunching area, the maximum stalk-deflection and the secondary cutting length etc., the adequate cutting velocity at forward speed of 0.5㎧ to 1.2㎧ was from 0.3㎧ to 0.96㎧ for the double cutting knives.

• 화훼연구
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• 제17권3호
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• pp.165-171
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• 2009

팔레높시스 화경의 모든 부위를 사용한 화경절편조직을 기내 배양하여 효율적이고 변이 발생률이 낮은 균일한 식물체를 일시에 대량생산하기 위하여 Hyponex와 생장조절물질 TDZ, BA의 적정 농도를 규명하고자 하였다. 두 공시 품종은 Hyponex, TDZ, BA 농도별로 floral stem, shoot, 다신초의 기관형성 발생에서 차이를 보였다. 팔레높시스의 화경절편조직 배양에 적절한 Hyponex의 농도는 $4g{\cdot}L^{-1}$ 가 가장 효과적이었으며, 다신초 형성에 효과적인 TDZ의 농도는 $0.3mg{\cdot}L^{-1}$이고, BA의 농도는 $5mg{\cdot}L^{-1}$ 이었다. 화경절편조직 배양 시 Hyponex $4g{\cdot}L^{-1}$을 기본배지로 하여 TDZ와 BA의 효과를 비교한 결과 TDZ가 더 효과적이었다.

• 한국버섯학회지
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• 제7권2호
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• pp.45-48
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• 2009

느타리버섯 재배에 있어서 부레옥잠과 바나나 잎, 줄기는 단독으로 배지재료로 쓰기는 어려운 것으로 나타났다. 면실피를 주재료로 하고 부레옥잠과 바나나 잎, 줄기를 첨가재료로 사용할 경우 부레옥잠을 20% 혼합하였을 때 115mm로 대조구와 같은 균사생장속도를 나타냈고 50%를 혼합하였을 때 103mm로 균사생장속도는 약간 느리지만 균사밀도가 높아 50%까지 대체 사용할 수 있음을 확인하였다. 바나나는 20%를 혼합하였을 때 106mm의 생장속도를 보였고 그 이상의 혼합비에서는 균사생장속도가 늦어지고 균사밀도가 약해졌다. 이는 부레옥잠에서는 주재료의 대체 비율이 20%이상 50%미만 이었고, 바나나 잎, 줄기에서는 20%미만 이었다. 부레옥잠과 바나나 잎, 줄기는 대체배지로 그 생산량이 많고 저가로 구입할 수 있기 때문에 앞으로 더 많은 연구와 실증재배를 해 볼 필요가 있다.

• 한국연초학회지
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• 제5권2호
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• pp.63-69
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• 1983

The tensile strength (Pm) . elongation percentage ($\varepsilon$) , elastic modulus (I) , and specific volume (Sv) were calculated from the data of tensile characteristics of By 104 .and Burley 21 tobacco leaves, which were measured over a relative humidity from 60 to 80% at $20\pm2^{\circ}C$.The important results obtained were as follows.(1) Tensile strength(Pm) The "Pm"of By 104 was greater than that of Burley at. The "Pm"in both varieties were increased toward the upper stalk position and according to the decrease of relative humidity. The "Pm" of parallel piece to tile vein in a leaf were greater than that of right angle piece to the vein. (2) Elongation percentage ($\varepsilon$). The "s" of By 104 was higher than that of Burley 21. The "s" in both varieties were increased toward the upper stalk position and according to the increase of relative humidity. The "s" of parallel Piece to the vein in a Burley leaf was greater than that of right angle piece to the vein. (3) Elastic modulus (E) The "E" of Burley 21 leaf was higher than that of By 104. The "E" in both varieties were increased toward the lower stalk position and according to decrease of relative humidity. The "E"of parallel piece to the vein in both varieties leaves were greater than that of right angle piece to tile vein. (4) Specific volume (Sv) The "Sv" of Burley 21 leaf was increased than that of By 104. The "Sv" in both varieties were increased toward the lower stalk position and according to the decrease of relative humidity.ot;Sv" in both varieties were increased toward the lower stalk position and according to the decrease of relative humidity.dity.

• 미생물학회지
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• 제22권1호
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• pp.57-66
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• 1984

Stigimatella aurantiaca는 calcium, barium, 또는 lithium이온이 포함된 배지에서는 빛을 비추어 주어도 stalk를 형성하지 않고 포자낭으로만 분화하였다. Calcium이온과 GMP가 함께 첨가된 배지(GMP 배지)에서는 빛의 유무에 관계없이 stalk와 포자낭을 지니는 fruiting body가 형성되었다. Calcium 이온과 pheromone 포함된 배지(pheromone배지)에서는 빛이 있는 경우에는 대부분의 세균들이 stalk로만 분화되었으나, 빛이 없는 경우에는 대부분 포자낭으로만 분화하였다. 세균집합체는 calcium과 potassium 및 sodium이 함꼐 첨가된 배지(CPS배지)에서보다 calcium 이온만 첨가된 배지 (calcium 배지)에서 더 많이 형성되었다. GMP 배지나 pheromone 배지에서는 calcium 배지에서보다 적은 수의 세균집합체가 형성되었고, 빛이 없는 경우에는 이 집합체의 형성이 소요된 시간이 단축되었다. 빛은 상기한 네가지 배지에서 공히 짧은 시간에 더 많은 세균집합체가 형성되도록 하였다.

• 한국작물학회지
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• 제28권2호
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• pp.252-257
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• 1983

버어리종 대말림시 백화엽발생을 방지하고 건조기간을 단축하기 위하여 시험을 수행하였던 바, 결과를 요약하면 다음과 같다. 1. 건조실내 온도는 흑색비닐피복구 및 환기창이용구가 관행구보다 $3^{\circ}C$정도 높았으며, 탈수속도는 환기창이용구가 훨씬 빨랐다. 2. 백화엽발생율은 완전대말림구가 높았고, 지면을 30cm 판 처리가 낮았다. 3. 수량감소는 완전대말림구가 컸다. 4. 품질 및 대금은 환기창이용구 및 지면을 30cm판 구가 높았다. 5. 물리성은 줄말림용 차광막처리가 비교적 양호하였다. 6. 백화엽발생을 억제하기 위하여는 첫째, 본포에서 치마름엽과 과숙엽을 방지하고, 둘째, 하위엽은 1-2회 수확한 후 대베기를 실시하며, 셋째, 갈변후에는 건조기간을 단축시키기 위한 온습도관리에 유의해야 할 것으로 생각된다. 7. 버어리종 담배의 물리성을 향상시키면서 건조중 백화의 원인이 되는 산소 및 수분과의 반응을 억제시킬 수 있는 방법에 대하여는 앞으로 더욱 검토가 요구된다.

• 식물조직배양학회지
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• 제28권4호
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• pp.189-195
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• 2001

화경절 액아 유래 신초의 잎을 이용한 팔레높시스 대량번식에서 배양재료로 이용되는 신초의 발생수를 증가시키기 위해 화경배양을 위한 배지, 배양온도 화경 전처리 등 기내 배양조건과 계통 간 화경의 발육형태에 대해 조사하였다. 화경절 액아의 신초발생률은 MS배지에서 90.9%, Hyponex 배지에서 54.4%이었다. 5 mg/L의 BA가 첨가된 MS배지에서 신초가 많이 형성되었으며 발육상태도 양호하였다. 화경 액아의 위치별 발육형태는 기저부 쪽일수록 신초발생이, 상부 쪽일수록 2차화경이 많이 발생되었으며 25~28$^{\circ}C$의 배양온도는 액아로부터 신초를 얻는 데 적절하였으며, 황화처리는 오염률을 낮추는 데 효과가 있었으나 페놀물질 유출량의 감소효과는 없었다. 수집한 30계통 중 27계통의 화경에서 신초를 얻었으며 화경편 당 평균 신초수는 3.17개이었으나 Phal. 3020, 3039 등은 신초가 많이 발생되어 다른 계통과 차이가 있었다. 교잡 F$_1$계통의 화경발육 양상은 모본(수집계통)과 비슷하였다.