• Title/Summary/Keyword: stability of treatment

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Effect of Glutaraldehyde Treatment on Stability of Permeabilized Ochrobactrum anthropi SY509 in Nitrate Removal

  • Park, Young-Tae;Park, Jae-Yeon;Park, Kyung-Moon;Choi, Suk-Soon;Yoo, Young-Je
    • Journal of Microbiology and Biotechnology
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    • v.18 no.11
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    • pp.1803-1808
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    • 2008
  • For practical application, the stability of permeabilized Ochrobactrum anthropi SY509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. As the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. However, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. Thus, whole-cell immobilization using glutaraldehyde was performed, and 0.1% (v/v) glutaraldehyde was determined as the optimum concentration to maintain activity and increase the half-life. It was also found that 0.1% (v/v) glutaraldehyde reacted with 41.9% of the total amine residues on the surface of the cells during the treatment. As a result, the half-life of the permeabilized cells was increased from 90 to 210 days by glutaraldehyde treatment after permeabilization, and no cell viability was detected.

A Comparison Study on Functional Properties of Peanut Protein and Chemically Modified Peanut Protein (분리 땅콩 단백질과 화학적으로 수식화한 단백질간의 식품학적 특성 비교)

  • Sohn, Kyung-Hee;Min, Sung-Hee;Park, Hyun-Kyung;Park, Jin
    • Korean journal of food and cookery science
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    • v.7 no.2
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    • pp.97-104
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    • 1991
  • This study was carried out in order to study the protein functionality such as foaming and emulsifying properties by succinylation of peanut protein isolates. Succinylated and unsuccinylated peanut protein isolate was tested for to find out the effect of pH, heat treatment and sodium chloride concentration on the solubility, foam expansion, foam stability, emulsion capacity and emulsion stability. The results are summarized as follows; 1. Succinylation enhanced the solubility of peanut protein isotate (PPI). The solubility of succinylated PPI markedly increased at pH 4.5. When the protein solutions was heated, the solubility of succinylated PPI greatly increased than PPI at pH 3. With addition of NaCl, solubility of succinylated PPI increased at pH 7 and pH 9. 2. The foam expansion of PPI and succinylated PPI on pH was no difference between both proteins. Addition of NaCl and heat treatment caused steeply increased in foam expansion at pH 3. 3. The foam stability of PPI and succinylated PPI showed the lowest value at pH 4.5. When PPI and succinylated PPI was heated, foam stability of two proteins incensed at pH 3 and showed similar aspects between PPI and succinylated PPI. However, at pH 9 stability of succinylated PPI decreased by heat treatment over $60^{\circ}C$. 4. Emulsion capacity of succinylated PPI on pH was markedly increased and showed the highest value at pH 11. At pH 4.5 which is isoelectric point of PPI, emulsion capacity of PPI by succinylation improved than that of PPI. When succinylated PPI was heated, emulsion capacity was greatly increased at pH 2 and pH 7. With NaCl was added, emulsion capacity of succinylated PPI increased than that of PPI. 5. Emulsion stability of PPI and succinylated PPI was affected by pH and showed its highest value at pH 11. At pH 4.5, emulsion stability of succinylated PPI increased than that of PPI. Addition of NaCl and heat treatment caused slightly increased in emulsion stability of succinylated PPI.

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Effect on Stability of Ginseng Saponins by Various Physical and Chemical Treatments (물리화학적 처리가 인삼 Saponin의 안정성에 미치는 영향)

  • 도재호;장진규
    • Journal of Ginseng Research
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    • v.10 no.2
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    • pp.193-199
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    • 1986
  • To investigate the stability of ginseng saponin, various physical and chemical treatments for red ginseng alcohol extract (70% ethyl alcohol) were carried out, and then the variations of ginseng saponin in extract were investigated by high performance liquid chromatography (HPLC) method. Irradiation of ${\gamma}$-ray, and ultraviolet ray, sonocatalysis by ultrasonicator, treatment of electronic range, catalytic ozonation did not or slightly affect degradation of ginseng saponins, but they were degraded by heat treatment.

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Thermal Stability of Al-Fe-X Alloy System Prepared by Mechanical Alloying and Spark Plasma Sintering: I. Al-Fe (기계적 합금화 및 스파크 플라즈마 소결에 의해 제조된 Al-Fe-X계 합금의 열적 안정성: I. Al-Fe)

  • Lee, Hyun-Kwuon;Lee, Sang-Woo;Cho, Kyeong-Sik
    • Journal of Powder Materials
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    • v.12 no.1
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    • pp.70-78
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    • 2005
  • Mechanical alloying using high-energy ball mill and subsequent spark plasma sintering (SPS) process was applied to understand mechanical alloying processing of Al-Fe alloy system. The thermal stability of mechanically alloyed Al-Fe alloy was intended to be enhanced by SPS process. Various analytical techniques including particle size analysis, density measurement, micro-Vickers hardness test, SEM, TEM, and X-ray diffractometry were adopted to find optimum processing conditions for mechanical alloying and subsequent SPS and to estimate thermal stability of the prepared alloy. It was found from the treatment of mechanically alloyed Al-8wt.%Fe powder mixture that needle-shaped $Al_3Fe$ precipitates was formed in the Al-Fe matrix, and the alloy compact showed enhanced densification and reached its full density with little loss of its fine microstructure. After heat treatment at $500^{\circC}$, it was also shown that the thermal stability of Al-8wt.%Fe alloy fabricated in the present study was enhanced, which was due to its fine microstructure developed by fast densification of SPS.

Screening of Antimicrobial Activity and Proteolytic Enzyme Stability of Extract of the Blue Mussel Mytilus edulis (진주담치(Mytilus edulis) 추출물의 항균활성 및 단백질 분해효소에 대한 안정성 탐색)

  • Lee, Ji-Eun;Seo, Jung-Kil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.3
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    • pp.280-286
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    • 2021
  • This study was performed to screen the antimicrobial activities and proteolytic enzyme stability of the acidified extract of the Blue mussel Mytilus edulis. The acidified extract showed potent antimicrobial activities against Gram-positive bacteria, Bacillus subtilis, and Gram-negative bacteria, Escherichia coli D31, but had no activity against Candida albicans. Treatment of extract with trypsin completely abolished all or significant antibacterial activity against the tested bacteria, but slightly decreased antimicrobial activity against B. subtilis, and treatment of extract with chymotrypsin retained almost antibacterial activity against the tested bacteria except for E. coli D31. To confirm the additional enzyme stability of the extract, antimicrobial activity of the extract was tested after treated with several enzymes. Enzymes treated extract showed potent antimicrobial activity against B. subtilis and its activity was also retained for 5 h after trypsin treatments. Non-proteinaceous materials in the acidified extract also showed strong DNA-binding ability but did not show bacterial membrane permeabilizing ability. All our results indicate that mussel extract might contain the proteinaceous or non-proteinaceous antibacterial materials target not bacterial membrane but intracellular components. These results could be used to develop mussel extract as an additive for the improvement of stability or antimicrobial activity of antibiotics against specific bacteria.

A Study on the Evaluation of Long Term Stability of Brinell Standard Hardness Tester and Automatic Indentation Measurement System and Optimum Test Condition (브리넬 경도 표준 시험기 및 압입자국 자동 측정 장치의 장기 안정도 평가와 최적 시험조건에 관한 연구)

  • Bahng, G.W.;Tak, Nae-Hyung;Hwang, N.M.
    • Journal of the Korean Society for Heat Treatment
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    • v.13 no.1
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    • pp.10-15
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    • 2000
  • Evaluation of long term stability of the Brinell standard hardness tester was carried out to secure its application as a national standard in Brinell hardness. Accuracy and repeatability in load application were tested through evaluating errors in hardness measurement of certified reference blocks. All of those requirements in KS as well as ISO specifications were satisfied by this standard hardness tester. In addition to this, long term stability test of automatic indentation measurement system was carried out. The scattering range was almost the same with its error range. To figure out an optimum test condition for better repeatability and long term stability, the effect of load variation, load application speed and time have been studied using orthogonal array experimental plan. It was found that the best combination is $30{\mu}m/s$ of load application speed and 25 seconds of load application time.

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Thermal Stability of the Interface between TaN Deposited by MOCVD and Electroless-plated Cu Film (MOCVD 방법으로 증착된 TaN와 무전해도금된 Cu박막 계면의 열적 안정성 연구)

  • 이은주;황응림;오재응;김정식
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.11 no.12
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    • pp.1091-1098
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    • 1998
  • Thermal stability of the electroless deposited Cu thin film was investigated. Cu/TaN/Si multilayer was fabricated by electroless-depositing Cu thin layer on TaN diffusion barrier layer which was deposited by MOCVD on the Si substrate, and was annealed in $H_2$ ambient to investigate the microstructure of Cu film with a post heat-treatment. Cu thin film with good adhesion was successfully deposited on the surface of the TaN film by electroless deposition with a proper activation treatment and solution control. Microstructural property of the electroless-deposited Cu layer was improved by a post-annealing in the reduced atmosphere of $H_2$ gas up to $600^{\circ}C$. Thermal stability of Cu/TaN/Si system was maintained up to $600^{\circ}C$ annealing temperature, but the intermediate compounds of Cu-Si were formed above $650^{\circ}C$ because Cu element passed through the TaN layer. On the other hand, thermal stability of the Cu/TaN/Si system in Ar ambient was maintained below $550^{\circ}C$ annealing temperature due to the minimal impurity of $O_2$ in Ar gas.

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Survey of the Secondary Effluents from Municipal Wastewater Treatment Plants in Korea (우리나라 하수처리장 방류수 수질현황 및 특성)

  • Kim, Youngchul;An, Ik-Sung;Kang, Min-Gi
    • Journal of Korean Society on Water Environment
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    • v.21 no.2
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    • pp.158-168
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    • 2005
  • In this study, the discharging effluents from have been 9 municipal wastewater treatment plants surveyed for 1 year-period. Statistics including probability distribution, cumulative occurrence concentration and other statistical parameters were presented. In addition, treatment performance and its stability were also discussed. Most of the plants, have an operational problem of high soluble organic content in the secondary effluent which may be associated with the integrated treatment of human and livestock manures. Nitrogen concentration in the effluents were usually higher during the period of summer and winter. It was found that this is mainly due to lack of the proper C/N ratio during the summer, or/and the effects of low temperature and less dilution by dry weather during the winter. Phosphorus concentration is sharply increased in June. Discussion with plant operators told that it is due to the dissolution of phosphate from the sludge accumulated in the primary settling tanks from the early spring to june. During this period, usually, sludge treatment line is highly overloaded with flush-outs of the sediments also stored in the bottom of combined sewer due to the low flow during winter season. Most of the plants can meet new effluent discharge limits of the nitrogen and phosphorus, and total coliform without further treatment.

Change in Microstructural Stability of AZ31 Alloy By the Addition of CaO (CaO 첨가에 의한 AZ31 합금 미세조직의 열적 안정성 변화)

  • Jun, Joong-Hwan
    • Journal of the Korean Society for Heat Treatment
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    • v.26 no.3
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    • pp.113-119
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    • 2013
  • Grain growth behaviors of hot-rolled AZ31 (Mg-3%Al-1%Zn) and AZ31-0.3%CaO alloys at elevated temperatures have been investigated in order to clarify the effect of CaO addition on grain stability of Mg-Al-based wrought alloy. The grain size of CaO-free alloy increased steeply from 673 K with an increase in annealing temperature from 573 to 773 K, whereas the grains of CaO-containing alloy were relatively stable up to 723 K. The activation energies for grain growth ($E_g$) were 12.2 and 18.3 kJ/mole between 573 and 673 K and 119.2 and 126.9 kJ/mole between 673 and 773 K in the AZ31 and AZ31-0.3%CaO alloys, respectively. This result indicates that grains in the CaO-added alloy possess higher thermal stability than CaO-free alloy. SEM observations on the annealed alloy samples revealed that higher grain stability resulting from CaO addition would be associated with the suppression of grain growth by Ca-related precipitate particles distributed in the microstructure.

Analysis of Process and Operating Characteristics for Chung Nam Province Sewage Treatment Plants (충청남도 하수처리시설의 공정 및 운영 특성 분석)

  • Oa, Seong Wook;Lee, Sang Jin
    • Journal of Korean Society on Water Environment
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    • v.25 no.4
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    • pp.553-559
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    • 2009
  • Currently, small scale sewage works are getting increase in Chung Nam Province and it is strongly required for those plants to get the information of optimized procedures and technologies. Most processes for sewage works in Korea were designed for large scale plants, so many difficulties are observed in small scale sewage works. This study was conducted to evaluate the propriety of O&M and construction cost for sewage treatment plants in Chung Nam Province. The treatment results and process stability of 32 public sewage treatment plants were also investigated. It is expected to provide optimum O&M and construction cost for future small scale sewage works and improving projects of existing plants by these results. Pollution problems caused by small scale plants are usually restricted to small areas; however, in view of the high cost per unit population, treatment requirements and alternatives have to be studied carefully. In comparison to larger plants, more pronounced and different boundary conditions such as unstable influent load, per capita costs and a large variety of feasible treatment and disposal systems were considered.