Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Glutaraldehyde Treatment on Stability of Permeabilized Ochrobactrum anthropi SY509 in Nitrate Removal

  • Park, Young-Tae (Graduate Program in Biochemical Engineering and Biotechnology, Seoul National University) ;
  • Park, Jae-Yeon (School of Chemical and Biological Engineering, Seoul National University) ;
  • Park, Kyung-Moon (Department of Chemical System Engineering, Hongik University) ;
  • Choi, Suk-Soon (Department of Biological and Environmental Engineering, Semyung University) ;
  • Yoo, Young-Je (Graduate Program in Biochemical Engineering and Biotechnology, Seoul National University)
  • Published : 2008.11.30
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Abstract

For practical application, the stability of permeabilized Ochrobactrum anthropi SY509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. As the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. However, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. Thus, whole-cell immobilization using glutaraldehyde was performed, and 0.1% (v/v) glutaraldehyde was determined as the optimum concentration to maintain activity and increase the half-life. It was also found that 0.1% (v/v) glutaraldehyde reacted with 41.9% of the total amine residues on the surface of the cells during the treatment. As a result, the half-life of the permeabilized cells was increased from 90 to 210 days by glutaraldehyde treatment after permeabilization, and no cell viability was detected.

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References

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