• Title/Summary/Keyword: stability control

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Production of Pyruvate Dehydrogenase Complex-E2 Specific Human Monoclonal Antibody in Fed-batch Culture Systems with High Cell Density Recombinant Escherichia coli (고농도 재조합 대장균의 Fed-batch 배양 시스템을 이용한 Pyruvate Dehydrogenase Complex-E2 특이성 인간 모노클론 항체의 생산)

  • 이미숙;전주미;차상훈;정연호
    • KSBB Journal
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    • v.15 no.5
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    • pp.489-496
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    • 2000
  • Several culture systems including batch, two-stage CSTR, semi-fed batch, and two-stage cyclic fed-batch were investigated for the efficient production of the Fab fraction of PDC-E2 specific human monoclonal antibody using high cell density recombinant E. coli. A two-phase batch system and a two-stage continuous system were examined to overcome plasmid instability problems, by separating the growth and the production stages. The cell density and productivity of the two-stage continuous culture was better than that of the two-phase batch fermentation. In the two-stage continuous culture system with DO-stat, the cell growth and the productivity were superior to those of the system without the DO control. Also, almost total plasmid stability was maintained in the two-stage continuous culture system. Modified M9 medium was selected as an optimum feeding medium for the fed-batch process, and the optimum C/N ratio determined to be 2:3. The optimum feeding rate was $0.6g/\ell/hr$ for a constant feeding strategy in semi-fed batch system. When the feeding medium was fed by pulsing, it was observed that more frequent pulsing resulted in improved cell growth. The linear feeding method was the most efficient of the various feeding methods tested. Finally, high cell density culture using a two-stage cyclic fed batch system with pH-stat was tried because the linear feeding method showed limitations in terms of obtaining high cell densities, and a cell density of $54 g/\ell$ was achieved. It was concluded that the two-stage cyclic fed batch system was the most efficient system for high cell density culture of the systems tested. However, productivity improvements were lower than expected due to the extremely high accumulations of acetate, although the low levels of residual glucose were maintained.

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Proper Growing Regions and Management Practices for Improving Production Stability in Direct-seeded Rice Cultivation (벼 무논직파 재배 안정성 확보를 위한 조건 및 재배적지 설정)

  • Hwang, Woon-Ha;Jeong, Jae-Hyeok;Lee, Hyen-Seok;Yang, Seo-Yeong;Lee, Chung-Keun;Lim, Yeon-Hwa;Cho, Seung-Hyun;Min, Hyun-Kyung;Kim, Sang-Kuk;Nam, Jin-Woo;Choi, Yeo-Seul;Jo, Youn-Sang;Choi, Kyung-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.4
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    • pp.336-343
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    • 2019
  • Wet direct-seeding (WDS) is an important method for improving the competitiveness of rice production in South Korea. We analyzed the optimum direct-seeding date to establish the rice standing rate in each area and selected suitable areas for WHS by considering the heading stage limit date for improving cultivation safety. As a result, the rice direct-seeding date to control weedy rice was around 5.15, 5.17-5.19, and after 5.20 in southern Youngnam, southern Honam, and the Middle Coast areas, respectively. However, the optimum seeding date for good standing rice was in late March in most areas. Analyzing by area, most of the southern plains and parts of the central inland plain are suitable for WHS. However, most parts of Gwangwon-do, and the northern parts of Chungbuk, Gyeongbuk, and Yeonghonam areas are not suitable for WHS, and should therefore avoid WHS.

Partial transmission block production for real efficient method of block and MLC (Partial transmission block 제작 시 real block과 MLC를 이용한 방법 중 효율적인 방법에 대한 고찰)

  • Choi JiMin;Park JuYoung;Ju SangGyu;Ahn JongHo
    • The Journal of Korean Society for Radiation Therapy
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    • v.16 no.2
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    • pp.19-24
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    • 2004
  • Introduction : The Vaginal, the urethra, the vulva and anal cancer avoid the many dose to femur head and the additional treatment is necessary in inguinal LN. The partial transmission block to use inguinal LN addition there is to a method which it treats and produce partial transmission block a method and the MLC which to it analyzes. Material & Methode : The Inguinal the LN treatment patient partial transmission it used block and the MLC in the object and with solid water phantom with the patient it reappeared the same depth. In order to analyze the error of the junction the EDR2 (Extended dose range, the Kodak and the U.S) it used the Film and it got film scanner it got the beam profile. The partial transmission block and the MLC bias characteristic, accuracy and stability of production for, it shared at hour and comparison it analyzed. Result : The partial the transmission block compares in the MLC and the block production is difficult and production hour also above 1 hours. The custom the block the place where it revises the error of the junction is a difficult problem. If use of the MLC the fabrication will be break and only the periodical calibration of the MLC it will do and it will be able to use easily. Conclusion : The Inguinal there is to LN treatment and partial transmission block and the MLC there is efficiency of each one but there is a place where the junction of block for partial transmission block the production hour is caught long and it fixes and a point where the control of the block is difficult. like this problem it transfers with the MLC and if it treats, it means the effective treatment will be possible.

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A Study on the Appropriate Management of Maritime Police Authority in Korea Coast Guard: Focusing on the Japan Coast Guard (해양경비안전본부의 해양경찰권 적정 운영방안에 관한 연구: -일본 해상보안청과의 비교를 중심으로-)

  • Son, Yeong-Tae
    • Korean Security Journal
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    • no.42
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    • pp.361-391
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    • 2015
  • Regarding the [Government Organization Act; which is legislated on 19th November, 2014] Korea Coast Guard(KCG) has been re-organized and belong from Korea Coast Guard shall be established under the Minister of Oceans and Fisheries to Ministry of Public Safety and Security. Furthermore, National Police Agency(NPA) Commissioner has the right for administer duties concerning investigation and information by succession from Korea Coast Guard Commissioner. That means that main rule has been moved from prior KCG to Ministry of Public Safety and Security(MPSS) and NPA currently which is dual structure. Meanwhile, This kind of organization change has been effective to investigative agency which exert KCG's call of duty and causes needs of variety problems. In other words, There are quite huge changes such as KCG's reduction of their work, call of duty and re-organization regarding revised government organization act. However this change - including re-organization by government, was not able to take current MPSS's special features such as organization specialty and legal rights. It means, the current change has not been taken present law system CRIMINAL PROCEDURE LAW and there was no preparation to stable maritime police authority action as well. To sum up, this revised GOVERNMENT ORGANIZATION ACT is supposed to provide total, quick security service by establishing strong disasters and safety control tower. However they only contains few area such as organization revision regarding 'Sewol Ferry Disaster', they was not able to contain the other parts of Society. Therefore, in this article I would like to check the part of re-evaluation of current change made by KCC's organization revision. It is supposed to provide better legal stability by making clear of work area by government agencies who acts maritime police authority.

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Laterally Unbraced Length for Preventing Inelastic Lateral-Torsional Buckling of High-Strength Steel Beams (고강도 강재보의 비탄성 횡-비틀림좌굴 제어를 위한 횡지지 거리)

  • Park, Chang Hee;Lee, Cheol Ho;Han, Kyu Hong;Kim, Jin Ho;Lee, Seung Eun;Ha, Tae Hyu;Kim, Jin Won
    • Journal of Korean Society of Steel Construction
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    • v.25 no.2
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    • pp.115-130
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    • 2013
  • In this study, lateral-torsional buckling (LTB) strength of high-strength H-beams built up from 800MPa tensile-strength steel was experimentally and analytically evaluated according to current lateral stability provisions (KBC 2009, AISC-LRFD 2010). The motivation was to evaluate whether or not current LTB provisions, which were originally developed for ordinary steel with different stress-strain characteristics, are still applicable to high-strength steel. Two sets of compact-section specimens with relatively low (Set A) or high (Set B) warping stiffness were prepared and tested under uniform moment loading. Laterally unbraced lengths of the test specimens were controlled such that inelastic LTB could be induced. All specimens exhibited LTB strength exceeding the minimum limit required by current provisions by a sufficient margin. Moreover, some specimen in Set A reached a rotation capacity required for plastic design, although its laterally unbraced length belonged to the inelastic LTB range. All the test results indicated that extrapolation of current provisions to high-strength steel is conservative. In order to further analyze the test results, the relationship between inelastic moment and laterally unbraced length was also derived in explicit form for both ordinary- and high-strength steel based on the effective tangent modulus of inelastic section. The analytical relationship derived again showed that extrapolation of current laterally unbraced length limit leads to a conservative design in the case of high-strength steel and that the laterally unbraced length to control the inelastic LTB behavior of high-strength steel beam should be specified by including its unique post-yield strain-hardening characteristics.

Study on Ventilation Efficiency of A Naturally Ventilated Broiler House-( I ) Summer Season (자연환기식 육계사내의 환기효율성 조사연구-( I )하절기)

  • 이인복;유병기;정문성;윤진하;전종길;김경원;성시흥
    • Journal of Animal Environmental Science
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    • v.9 no.1
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    • pp.9-18
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    • 2003
  • Most of broiler houses in Korea have the trouble of environmental control such as suitability, stability, and uniformity of internal climate, resulting in serious stress on chickens. Accordingly, it is very urgent to develop optimum designs of naturally and mechanically ventilated broiler houses for Korean climate. In this study, the internal climates such as air temperature, humidity, dust, ammonia gas, and air velocity were measured at a naturally ventilated broiler house. The data were collected during summer season including local weather data. It was found that the difference between measured and optimum air temperatures was $14.0^{\circ}C$ in maximum during the summer time. The daily maximum range of internal averaged air temperature was found $10.5^{\circ}C$ while the uniformity was $5.2^{\circ}C$ in maximum. The maximum, average, and minimum internal averaged relative humidity were 89.3%, 73.7%, and 49.2%, respectively while locally measured were 95.1% and 47.2%, respectively in maximum and minimum. Considering Temperature-humidity index, during summer season, over 97% of totally rearing period was shown that counter plan is needed for thermal stress while it was very dangerous situation for 22% of the rearing period. The ammonia gas and dust concentrations were seriously affected by the broilers activity, growth level, and relative humidity.

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Quality Characteristics of Bread Added with Prickly Pear(Opuntia ficus-indica) Powder (손바닥선인장 열매 분말 첨가 식빵의 품질 특성)

  • Shin Doo-Ho;Lee Yeon-Wha
    • The Korean Journal of Food And Nutrition
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    • v.18 no.4
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    • pp.341-348
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    • 2005
  • The quality characteristics of bread added with the levels of $0\%,\;1.0\%,\;2.0\%\;3.0\%\;and\;4.0\%$ prickly pear powder were investigated. Mixing water absorption capacity was increased by increasing amounts of prickly pear powder. Mixing time of dough was increased compared to that of wheat flour by added prickly pear powder, while it decreased as prickly pear powder content increased. Dough stability of wheat flour added with $1.0\%$ prickly pear powder was similar to wheat flour dough, while decreased by increasing amount of prickly pear powder at the range of $2.0\~4.0\%$. Pasting temperature($66.1\~66.9^{\circ}C$) of wheat flour added with $1.0\%,\;2.0\%\;3.0\%\;and\;4.0\%$ prickly pear powder was lower($67.8^{\circ}C$) than that of wheat flour. Peak viscosity($2,226\~2,375$ cp) of wheat flour added with $1.0\%,\;2.0\%\;3.0\%\;and\;4.0\%$ prickly pear powder were higher(2,288 cp) than control, and increased with increasing amount of prickly pear powder Setback($797\~750$ cp) of wheat flour added with $1.0\%,\;2.0\%\;3.0\%\;and\;4.0\%$ prickly pear powder were lower(803 cp) than that of wheat flour, and decreased with increasing amount of prickly pear powder. The volume of dough added with prickly pear powder decreased compared to that of the wheat dough. The specific loaf volume of bread added with prickly pear powder decreased($8.0\~18.5\%$) compared to that of the wheat bread. Color of crumb was deep red gradually with the increasing amount of substituted prickly pear powder. Hardness, adhesiveness, gumminess and chewiness of bread were increased gradually with the increasing amount of substituted prickly pear powder but the springiness was decreased. The sensory evaluation showed that bread of wheat flour added with $2.0\%$ prickly pew powder was superiority than the wheat flour bread.

Conservation Scientific Diagnosis and Evaluation of Bird Track Sites from the Haman Formation at Yongsanri in Haman, Korea (함안 용산리 함안층 새발자국 화석산지의 보존과학적 진단 및 평가)

  • Lee, Gyu Hye;Park, Jun Hyoung;Lee, Chan Hee
    • Korean Journal of Heritage: History & Science
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    • v.52 no.3
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    • pp.74-93
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    • 2019
  • The Bird Track Site in the Haman Formation in Yongsanri (Natural Monument No. 222) was reported on the named Koreanaornis hamanensis and Jindongornipes kimi sauropod footprint Brontopodus and ichnospecies Ochlichnus formed by Nematoda. This site has outstanding academic value because it is where the second-highest number of bird tracks have been reported in the world. However, only 25% of the site remains after being designated a natural monument in 1969. This is due to artificial damage caused by worldwide fame and quarrying for flat stone used in Korean floor heating systems. The Haman Formation, including this fossil site, has lithofacies showing reddish-grey siltstone and black shale, alternately. The boundary of the two rocks is progressive, and sedimentary structures like ripple marks and sun cracks can clearly be found. This site was divided into seven formations according to sedimentary sequences and structures. The results of a nondestructive deterioration evaluation showed that chemical and biological damage rates were very low for all formations. Also, physical damage displayed low rates with 0.49% on exfoliation, 0.04% on blistering, 0.28% on break-out; however, the joint crack index was high, 6.20. Additionally, efflorescence was observed on outcrops at the backside and the northwestern side. Physical properties measured by an indirect ultrasonic analysis were found to be moderately weathered (MW). Above all, the southeastern side was much fresher, though some areas around the column of protection facility appeared more weathered. Furthermore, five kinds of discontinuity surface can be found at this site, with the bedding plane showing the higher share. There is the possibility of toppling failure occurring at this site but stable on plane and wedge failure by means of stereographic projection. We concluded that the overall level of deterioration and stability were relatively fine. However, continuous monitoring and conservation treatment and management should be performed as situations such as the physicochemical weathering of the fossil layer, and the efflorescence of the mortar adjoining the protection facility's column appear to be challenging to control.

Vietnam in 2017: The Situations and Prospects of Economics, Politics, and International Relations (베트남 2017: 경제, 정치, 대외관계의 현황과 전망)

  • CHAE, Su Hong;LEE, Han Woo
    • The Southeast Asian review
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    • v.28 no.1
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    • pp.21-51
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    • 2018
  • This article takes several approaches in explaining recent developments in Vietnam. First, it draws upon an array of sources that idealize Vietnam's embrace of capitalism and integration into the global market in order to sketch out its economy's progress in 2017. Second, it observes, evaluates, and diagnoses recent changes in the Vietnamese economy in the medium to long term by incorporating conflicting perspectives on Vietnam's performance as a capitalist country. Third, this article traces the power shifts that have risen from internal struggles in the Communist Party over political and social issues. Fourth, it elaborates on the aforementioned impact that foreign relations have had on socio-political developments in Vietnam, as well as the government's response. In so doing, it also attempts to evaluate, however briefly, the significance of the 25th anniversary of South Korea-Vietnam relations. Finally, it examines the public's reaction to the post-reform transitions in light of recent sociocultural changes. 2017 was a memorable year for Vietnam: a continuous march toward capitalism; the resulting expansion of the Vietnamese people's demands; political controversies and government control; the looming instability of United States-China relations and various attempts to address the situation. These events will inevitably replicate themselves in the future as the ostensibly socialist Vietnam adopts a capitalist model. The problem is that it is unclear whether these experiences will continue with the consent of the people of socialist Vietnam or engender resistance. It is difficult to achieve meaningful consent in the status quo of worsening inequalities, widespread corruption, monopoly on power, and sustained use of unskilled low-wage workers. In other words, when concerns such as welfare, public health, and the environment are set aside in favor of economic development and commercialization as they have been, discontent, rather than consent, will prevail. It is thus important to keep a watchful eye on the viability of the nominal economic growth, surface-level political stability, and strategic responses to foreign relations that took place in 2017.

The Development of Coin Circulation Institutes and their Regional Impact during the Reign of King Hyojong(孝宗) (효종조(孝宗朝) 행전사목(行錢事目)과 행전책(行錢策), 성과와 한계)

  • JUNG, Suhwan
    • (The)Study of the Eastern Classic
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    • no.73
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    • pp.153-184
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    • 2018
  • The aim of this thesis was to examine the circumstances that led up to successful coin use across the entire nation in 1678 (the $4^{th}$ year of King Sukjong's reign), during the Joseon Dynasty. To this end, this thesis analysed the Sa-Mouk(事目, Provisions) that contained the institutional protocol for coin circulation, implemented by King Hyojong and the statesman Kim Youk(金堉) who had practical experience in these matters over the ten years of King Hyojong's reign(1649-1659). To regulate the problematic wide circulation of coarse cotton cloth as currency in the market of 1650 (the $1^{st}$ year of King Hyojong's reign), prohibition measures were implemented. Besides the superficial justification given for these measures(i.e., that the market price was disturbed by the use of coarse cotton cloth), there was another purpose to prohibiting the circulation of cotton cloth as money, following the standard ruled by the government: the state aimed to ensure momentum for the upcoming coin circulation policy, by strengthening its control of the current economy. In 1651 (the $2^{nd}$ year of King Hyojong's reign), the government fully cracked down on the use of coarse cotton cloth as currency, and simultaneously implemented its coin circulation policy in the Pyeongan(平安) region. The pretext for this policy was to raise finances to support people who were starving as a result of poor harvests and famine. People who received coins from government officials could purchase food in the market, and the coin circulation policy was judged to be successful. Subsequently, to extend coin circulation further throughout the region, the Sa-Mouk for Seoul was established. The Sa-Mouk included stipulations regarding the use of coin in transactions and for government expenditure; it aimed thereby to enhance the national policy's market credit. The hasty implementation of the policy for the expansion of coin circulation caused some problems that required its modification. In 1652 (the $3^{rd}$ year of King Hyojong's reign), coin circulation was increased to encompass the Gyeonggi(京畿) region, and some of the tax that had been paid in rice was now paid in coin. However, coins were in short supply, since there was insufficient copper, the main material used in coin production, and the policy faced a significant limitation. Therefore, in 1655(the $6^{th}$ year of King Hyojong's reign), a new Sa-Mouk for coin circulation was established. This Sa-Mouk included specifications regarding the determination of coin values based on rice and silver, and mandated the wide spread installation of stores for exchanging spot goods for coins throughout the region in which coins were circulating. This policy's objective was to secure stability for the national economy by further regulating coin circulation. The sustained implementation of the coin circulation policy for ten years by King Hyojong and the statesman Kim Youk offered the government an opportunity to accumulate experience in coin circulation in the market, and also to learn from institutional trial and error. This may have been one of the contributing factors to the nation-wide coin circulation that was established in 1678. The objective of the policy implemented during King Hyojong's reign was not to meet the market's requirements, but rather to ensure the preservation of the national economy, and this misjudgement constituted the policy's key limitation. At this time, the government urgently needed to secure finances to cope with the war against China's Qing Dynasty.