• Title/Summary/Keyword: sseA

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Mutation of the invF Gene Encoding a Salmonella Pathogenicity Island 1 (SPI1) Activator Increases Expression of the SPI2 Gene, sseA (Salmonella Pathogenicity Island 1(SPI1)의 발현조절 유전자 invF의 변이가 SPI2 유전자(sseA)의 발현에 미치는 영향)

  • Han, Ah-Reum;Joe, Min-Ho;Kim, Dong-Ho;Baik, Sang-Ho;Lim, Sang-Yong
    • Microbiology and Biotechnology Letters
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    • v.40 no.1
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    • pp.70-75
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    • 2012
  • In Salmonella enterica, many genes encoded within Salmonella pathogenicity islands (SPI) 1 and 2 are required to cause a range of diseases in a variety of hosts. The SPI1-encoded regulator HilD activates both the SPI1 and 2 genes at different times during growth in Luria-Bertani (LB) media. In this study, the expression levels of hilD during growth in LB were investigated. The data suggest that hilD expression is induced in the early stationary phase and decreases in the late stationary phase, when sseA, an SPI2 gene, is maximally expressed. However, HilD could act as an activator of sseA expression in the late stationary phase despite being present at low levels. SseA expression was investigated in SPI1 regulator mutant strains, hilA, hilD and invF mutants. As expected, hilD mutation decreased sseA expression. However, we found that invF mutation caused a 1.5-fold increase in sseA expression in not only LB but also M9 minimal media, which is thought to resemble an intracellular environment. InvF overexpression restored sseA expression to wild-type levels in an invF mutant but did not cause an additional reduction in sseA expression. These results suggest that SPI1 controls SPI2 expression either positively or negatively.

Propagation of Structural Waves along Waveguides with Non-Uniformities Using Wavenumber Domain Finite Elements (국부적 불연속을 갖는 도파관을 따라 전파되는 파동에 대한 파수 영역 유한 요소 해석)

  • Ryue, Jungsoo
    • The Journal of the Acoustical Society of Korea
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    • v.33 no.3
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    • pp.191-199
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    • 2014
  • Wave reflection and transmission characteristics in waveguides are an important issue in many engineering applications. A combined spectral element and finite element (SE/FE) method is used to investigate the effects of local non-uniformities but limited at relatively low frequencies because the SE is formulated by using a beam theory. For higher frequency applications, a method named a combined spectral super element and finite element (SSE/FE) method was presented recently, replacing spectral elements with spectral super elements. This SSE/FE approach requires a long computing time due to the coupling of SSE and FE matrices. If a local non-uniformity has a uniform cross-section along its short length, the FE part could be further replaced by SSE, which improves performance of the combined SSE/FE method in terms of the modeling effort and computing time. In this paper SSEs are combined to investigate the characteristics of waves propagating along waveguides possessing geometric non-uniformities. Two models are regarded: a rail with a local defect and a periodically ribbed plate. In the case of the rail example, firstly, the results predicted by a combined SSE/FE method are compared with those from the combined SSEs in order to justify that the combined SSEs work properly. Then the SSEs are applied to a ribbed plate which has periodically repeated non-uniformities along its length. For the ribbed plate, the propagation characteristics are investigated in terms of the propagation constant.

Antioxidant Activities of Steamed Extract from Squid (Todarodes pacificus) Muscle

  • Lee, Woo-Shin;Kim, Yong-Tae;Byun, Hee-Guk
    • Preventive Nutrition and Food Science
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    • v.16 no.2
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    • pp.127-134
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    • 2011
  • The purpose of this study was to purify antioxidant substances from steamed squid extract (SSE). The yield of SSE was 8% by dry weight. The approximate compositions of SSE proteins, lipids, moisture, carbohydrate and ash were 64.95%, 1.69%, 7.23%, 4.44% and 21.69%, respectively. The major amino acids in SSE were taurine (29.17%), glycine (20.33%), alanine (12.51%), and glutamic acid (9.83%). Antioxidant activities were evaluated using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity, which was measured as 24.7% at 1.0 mg/mL. Four SSE fractions were isolated by Sephadex G-25 gel chromatography; the F2 fraction showed the highest DPPH radical scavenging activity. The F2 fraction was separated by reverse-phase high performance liquid chromatography (HPLC) using an octadecylsilane (ODS) column, yielding a purified antioxidant substance with a DPPH radical scavenging activity of 64.41% at 1.0 mg/mL, representing a 2.64-fold increase in the scavenging activity of SSE purified by the 3-step procedure. The amino acid compositions showed that purified SSE was rich in taurine, glycine, glutamic acid and alanine. The purified SSE significantly elevated 2',7'-dichlorodihydrofluororescein diacetate (DCFH-DA) fluorescence probe, which confirms its effective radical scavenging potential in cellular ROS. In addition, the SSE significantly inhibited oxidative damage of purified genomic DNA. These results suggest that a purified antioxidant substance from SSE can be used as a potential natural compound-based antioxidant in the functional food and pharmaceutical industries.

Development of ZnSSe:Te/ZnMgSSe DH structure Blue~Green tight Emitting Diodes (ZnSSe:Te/ZnMgSSe DH 구조 청색~녹색발광다이오드의 개발)

  • 이홍찬
    • Journal of Advanced Marine Engineering and Technology
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    • v.27 no.1
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    • pp.33-41
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    • 2003
  • The optical properties of $ZnS_ySe_{1-\chi-y}:Te_{\chi}(\chi<0.08,y~0.11)$ alloys grown by molecular beam epitaxy (MBE) have been investigated by photoluminescence (PL) and PL-excitation (PLE) spectroscopy. Good optical properties and high crystal quality were established with lattice match condition to GaAs substrate. At room temperature, emission in the visible spectrum region from blue to green was obtained by varying the Te content of the ZnSSe:Te alloy. The efficient blue and green emission were assigned to $Te_1 and Te_n(n\geq2)$cluster bound excitons, respectively. Bright green (535 nm) and blue (462 nm) light emitting diodes (LEDs) have been developed using ZnSSe:Te system as an active layer. The turn-on voltage of 2.1 V in current-voltage characteristics is very small compared to that of commercial InGaN-based LEDs (>3.4 V), indicating the formation of a good ohmic contact due to the optimized p-ZnSe/p-ZnTe multi-quantum well (MQW) superlattice electrode layers.

Size-of-source Effect and Self-radiation Effect of an Infrared Radiation Thermometer (적외선 복사온도계의 복사원 크기효과 및 자기복사효과)

  • Yoo, Yong-Shim;Kim, Bong-Hwak;Park, Chul-Woung;Park, Seung-Nam
    • Korean Journal of Optics and Photonics
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    • v.21 no.4
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    • pp.133-138
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    • 2010
  • All radiation thermometers have a size-of-source effect (SSE) and a self-radiation effect (SRE). The SSE,defined as dependence of the detector signal of a radiation thermometer on the diameter of a source, is critically dependent on the wavelength since diffraction is the main cause. In this paper, we have measured the SSE and the SRE of TRT2 (Transfer Radiation Thermometer 2, HEITRONICS) widely used as a transfer standard in low and middle temperature range. At $300^{\circ}C$, The radiation temperature difference between the 60 mm diameter blackbody and 10 mm diameter blackbody due to the SSE was estimated to be $3.5^{\circ}C$ in low temperature mode ($8-14\;{\mu}m$) and $0.5^{\circ}C$ in middle temperature mode ($3.9\;{\mu}m$). In addition, the measured radiation temperature difference of the blackbody due to the SRE was found to be 110 mK when the body temperature change of TRT2 was set at $2.6^{\circ}C$.

Enabling Dynamic Multi-Client and Boolean Query in Searchable Symmetric Encryption Scheme for Cloud Storage System

  • Xu, Wanshan;Zhang, Jianbiao;Yuan, Yilin
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.16 no.4
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    • pp.1286-1306
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    • 2022
  • Searchable symmetric encryption (SSE) provides a safe and effective solution for retrieving encrypted data on cloud servers. However, the existing SSE schemes mainly focus on single keyword search in single client, which is inefficient for multiple keywords and cannot meet the needs for multiple clients. Considering the above drawbacks, we propose a scheme enabling dynamic multi-client and Boolean query in searchable symmetric encryption for cloud storage system (DMC-SSE). DMC-SSE realizes the fine-grained access control of multi-client in SSE by attribute-based encryption (ABE) and novel access control list (ACL), and supports Boolean query of multiple keywords. In addition, DMC-SSE realizes the full dynamic update of client and file. Compared with the existing multi-client schemes, our scheme has the following advantages: 1) Dynamic. DMC-SSE not only supports the dynamic addition or deletion of multiple clients, but also realizes the dynamic update of files. 2) Non-interactivity. After being authorized, the client can query keywords without the help of the data owner and the data owner can dynamically update client's permissions without requiring the client to stay online. At last, the security analysis and experiments results demonstrate that our scheme is safe and efficient.

Effects of Dietary Supplementation of Shrimp Soluble Extract on Growth Performance, Innate Immunity and Digestibility of Pacific White Shrimp Litopenaeus vannamei (사료 내 새우가용성추출물(shrimp soluble extract) 첨가가 흰다리새우(Litopenaeus vannamei)의 성장, 면역력 및 소화율에 미치는 영향)

  • Ko, Daehyun;Shin, Jaehyeong;Kim, Min-Gi;Lee, Chorong;Kim, Sung-Sam;Park, Gunhyun;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.3
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    • pp.287-292
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    • 2021
  • This study was conducted to evaluate the effects of dietary supplementation with shrimp soluble extract (SSE) on growth performance, feed utilization, innate immunity and digestibility of Pacific white shrimp Litopenaeus vannamei. A basal diet (Con) was formulated and three other diets were prepared with SSE supplementation at different levels of 1, 2 and 4% (designated as SSE1, SSE2 and SSE4, respectively). Triplicate groups of shrimp (1.20±0.01 g) were fed one of the experimental diets for 6 weeks. At the end of the feeding trial, growth performance and feed utilization of the shrimp were significantly improved by dietary supplementation of SSE4 compared to those of shrimp fed the Con diet. Shrimp fed SSE4 diet had significantly higher phagocytic activity than shrimp fed the Con diet. Dietary supplementation of SSE improved the protein and dry matter digestibility of shrimp. These results indicate that SSE can be used as a functional additive in the diets for Pacific white shrimp.

Study of High-efficiency and Long-lived Blue - Green Light Emitting Diodes Using ZnSSe:Te System Grown by MBE (ZnSSe:Te계 청 -녹색 발광다이오드의 고효율화 및 장수명화에 관한 연구)

  • 이홍찬;이상태;이성근;김윤식
    • Proceedings of the Korean Society of Marine Engineers Conference
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    • 2002.05a
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    • pp.167-171
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    • 2002
  • We have investigated the optical properties of Te-doped ZnSSe:Te epitaxial layers grown on (100) GaAs substrates by molecular beam epitaxy. The Te-doped ternary specimen shows strong blue or green emission (at 300k) which is assigned to Te$_{1}$ or Te$_{n}$( n$\geq$2) cluster bound exciton. Bright green and blue light-emitting diodes (LEDs) have been developed using ZnSSe:Te system as an active layer. The green LEDs exhibit a fairly long device lifetime (>2000 h) when operated at 3 A/cm$^{2}$ under CW condition at room temperature. It is confirmed that the Te-doping induced "crystal-hardening effect" plays a significant role in both efficient and strong suppression of the optical device degradation.gradation.

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Hydroalcoholic Extract of Scrophularia Striata Attenuates Hypertrophic Scar, Suppresses Collagen Synthesis, and Stimulates MMP2 and 9 Gene Expression in Rabbit Ear Model

  • Zarei, Hatam;Tamri, Pari;Asl, Sara Soleimani;Soleimani, Meysam;Moradkhani, Shirin
    • Journal of Pharmacopuncture
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    • v.25 no.3
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    • pp.258-267
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    • 2022
  • Objectives: Hypertrophic scars (HSs) are caused by abnormal wound healing. To date, no standard treatment has been made available for HSs. Scrophularia striata has been reported to accelerate wound healing and has the potential to prevent HS formation. In this study, we investigated the anti-scarring effects of S. striata extract (SSE) in a rabbit ear model of scarring. Methods: In this study, New Zealand white rabbit (weight: 2.3-2.5 kg) were used. In the prevention phase of the study, three test groups received 5%, 10%, and 15% ointments of SSE in the Eucerin base, the fourth group received Eucerin, and the fifth group received no treatment. The samples were obtained on day 35 after wounding. In the treatment phase of the study, the test groups received an intralesional injection of SSE (5%, 10%, and 15%), the fourth group received an intralesional injection of triamcinolone, the fifth group received a solvent (injection vehicle), and the sixth group received no treatment. To evaluate the anti-scarring effects of SSE, the scar elevation index (SEI), epidermis thickness index (ETI), collagen deposition, and MMP2 and MMP9 gene expression were evaluated. Results: A significant reduction in SEI, ETI, and collagen deposition was noted in animals treated with SSE compared with the control groups. In addition, topical SSE stimulated MMP2 and MMP9 gene expression. Conclusion: The findings of this study demonstrate the potential for SSE in the prevention and treatment of HS. SSE could be prepared as an appropriate formulation to treat wounds and prevent abnormal scarring.

Design of the Successive Selection Encoder by the Logical Effort for High Flash Speed ADC's (고속 플래시 AD 변환기를 위한 Successive Selection Encoder의 Logical Effort에 의한 설계)

  • Lee Kijun;Choi Kyusun;Kim Byung-soo
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.42 no.4 s.334
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    • pp.37-44
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    • 2005
  • In this paper, a new type of the TC-to-BC encoder for high speed flash ADC's, called the Successive Selection Encoder (SSE), is proposed. In contrast to the conventional fat tree encoder based on OR operations, the W- outputs, in the new design, are obtained directly from TC inputs through simple MUX operations. The detailed structure of the SSE has been determined systematically by the method of the logical effort and the simulation oil Hynix 0.25um process. The theoretical and experimental results show that (1) it is not required to generate one-out-of-n signals, (2) the number of gates is reduced by the factor of 1/3, and (3) the speed is improved more than 2-times, compared to the fat tree encoder. It is speculated that the SSE proposed in this study is an effective solution for bottleneck problems in high speed ADCs.