• 한국해양바이오학회지
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• 제14권1호
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• pp.43-50
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• 2022

Squid is a sustainable source of long-chain omega 3 fatty acids. This study aims to assess the safety and triglyceride-lowering efficacy of refined oil derived from the squid(Todarodes pacificus) viscera. Male and female participants with elevated fasting serum lipids (i.e., total cholesterol of ≥5.2 mmol/L or fasting serum triglyceride of ≥1.65 mmol/L) were randomly allocated to the control (n = 52) or squid oil group (n = 52), and participants in the latter group were instructed to consume 3 g of squid oil daily for 60 days. None of the subjects reported adverse events associated with the consumption of squid oil. Baseline clinical chemistry and hematological parameter values and those toward the end of the treatment period were similar, and all values were within the normal range. Fasting cholesterol and triglyceride levels in the control and squid oil groups were similar; however, toward the end of the 60 day study period, these levels significantly reduced in the squid oil group relative to those in the control group (P< 0.01). However, high-density lipoprotein-cholesterol remained unchanged in both groups. Thus, it can be inferred that squid oil is a safe source of long-chain omega-3 fatty acids and has beneficial effects on the blood lipid levels. This is the first clinical study on squid oil usage, and suggests that it could be a sustainable source of omega 3 fatty acids.

• 한국해양바이오학회지
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• 제1권4호
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• pp.311-316
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• 2006

Enzymatic ethanolysis of fish oil with immobilized lipase was investigated for reducing the free fatty acid contents and enhancing the function of fish oil. Ethanolysis reactions were carried out in erlenmeyer flask (25ml) containing a mixture of squid viscera oil and 99.9% ethanol using 1% (based on w/w squid viscera oil) immobilized lipase. The reaction mixtures were incubated at $50^{\circ}C$ and shaken at 100rpm. Ethanol was added into the mixture by stepwise addition method of Shinmada[9]. Measurement of free fatty acid molar amounts was studied by Acid Value. Tendency of oil variation during transesterification was studied by TLC method. Enzymatic ethanolysis composed diglyceride, monoglyceride and fatty acid ethyl ester with reducing free fatty acid contents. Also, selective ethanolysis by Lipozyme TL-IM and Lipozyme RM-IM mostly did not react at the sn-2 position of squid viscera oil. Lipozyme RM-IM was more suitable enzyme to reduce the free fatty acid contents by ethanolysis than Lipozyme TL-IM. Squid viscera oil was transformed into suitable properties (5 in Acid Value) for functional fish oil production.

• 한국해양바이오학회지
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• 제2권3호
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• pp.155-159
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• 2007

Ethanolysis of squid viscera oil with immobilized lipase was investigated for reducing the free fatty acid contents and enhancing the function of the oil by stepwise addition method of Shinmada[1]. Tendency of oil variation during Ethanolysis showed increased content of diglyceride, monoglyceride and fatty acid ethyl ester with reduced free fatty acid contents. The oil composition was analyzed using GC-FID and compared before and after ethanolysis. Structural analysis of the lipid was performed by HPLC-UV spectrophotometer during ethanolysis. The transformed oil was thought to has suitable properties for functional oil production.

• 한국식품과학회지
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• 제40권6호
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• pp.621-625
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• 2008

오징어 간유 미세캡슐화 분말을 HPMC-FCC, zein-DP로 코팅하여 유동층 코팅 특성을 살펴본 결과, 유동층 코팅 효율은 HPMC-FCC, zein-DP 모두 90%의 효율을 나타내었다. 겉보기 밀도는 zein-DP 코팅분말이 0.6 g/mL로 높게 나타나 코팅 분말의 비중이 커져 흐름성이 개선되었음을 확인할 수 있었다. 인공위액과 인공장액에 대한 용해성을 살펴본 결과, HPMC-FCC는 각각 59.9%와 0%를 나타내었고 zein-DP는 각각 0%와 31.0%를 나타내어 코팅재료에 따라 용해성을 조절할 수 있었다. 저장 안정성은 zein-DP나 HPMC-FCC로 코팅한 분말이 미세캡슐화 분말보다 높은 PUFA/SFA 잔존율을 보여, 유동층 코팅 기술이 유지식품의 안정성을 확보할 수 있는 새로운 기술이 될 수 있음을 확인할 수 있었다.

• 한국해양바이오학회지
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• 제2권1호
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• pp.55-59
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• 2007

The oil and concentrated protein powder from squid viscera was extracted and recovered by a semi-batch supercritical carbon dioxide ($SCO_2$) extraction system and the degree of oxidation in the extracted oil was measured in order to compare with extracted oils using organic solvents. The degree of storage in treated squid viscera by $SCO_2$ extraction was measured in order to compare with untreated squid viscera. As results obtained, it was found that the auto-oxidation of the oils using $SCO_2$ extraction occurred very slowly compared to the oils by organic solvent extraction. And the treated squid viscera by $SCO_2$ extraction was reached the point of initial rottenness slowly than untreated squid viscera.

• 한국가금학회지
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• 제31권1호
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• pp.17-24
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• 2004

본 연구는 산란노계를 이용해 훈제닭을 만들기 위해서 실행되었다. 70∼72주령된 산란노계를 이용하여 시판용 일반사료 (대조구)에 100 IU의 $\alpha$-토코페롤과 10%오징어 간유를 썩은 사료 (처리구)를 15일간 급여시킨 후 도계하였다. 그리고 소우스액 침지, 삶기 및 시판용 훈연 소우스를 살포하는 훈연 과정을 거쳐서 새로운 제품을 만들었다. 훈제닭 (처리구) 중의 수분 및 조회분 함량은 대조구에 비해서 각각 증가하는 경향 및 유의하게 증가하였다 (P＜0.05). 훈제닭의 근육중의 EPA 및 DHA 함량은 100 IU $\alpha$-토코페롤과 10% 오징어 간유를 섭취 할 때 유의하게 높았다 (P＜0.01). 훈연닭 중의 아미노산의 함량은 100 IU $\alpha$-토코페롤과 10% 오징어 간유를 섭취하는 것에 의한 영향은 인정되지 않았다. 훈제닭에 대해서 500명 이상의 설문조사도 받았다. 이상의 결과를 고려할 때 산란노계를 이용한 훈제닭을 만드는 것이 가능해진 것으로 사료된다.

• 한국식품영양과학회지
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• 제36권1호
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• pp.100-104
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• 2007

피복물질과 오징어 간유의 혼합 비율에 따른 미세캡슐화 특성을 알아보았다. 유화액의 점도와 안정성은 피복물질의 함량이 높을수록 증가하는 경향을 나타내었고, 미세캡슐화 효율은 43.86$\sim$29.49%로 7:3>4:6>3:7>5:5>6:4의 순으로 나타났으나 7:3의 경우 분말내 oil 함량이 낮아 적절하지 않을 것으로 판단된다. 수분결합지수는 피복물질 함량이 낮을수록 낮은 수분결합력을 보였으며, 입자의 크기는 모두 10 ${\mu}m$ 이하로 나타났다. 지방산 조성은 모든 실험구에서 다가불포화지방산의 함량이 50% 이상이었으며, 또한 다가불포화지방산에 대한 포화지방산의 비율은 모든 혼합비가 2.10 이상으로 높게 나타났다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.769-772
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• 2000

강원도 동해안에서 다량 어획되어 가공되는 오징어 및 명태 내장으로부터 추출되는 내장유 용도 확대 및 고부가가치화를 위한 미세 캡슐화를 제조를 위한 조건을 시험하였다. 미세 캡슐 소재로 wall material은 gum arabic과 유화제로 gelatin이 적절하고, gum arabic과 gelatin을 소재로하여 배합비를 달리하였을 때, 전체적인 경향을 유화제가 wall material 보다 많은 것이 양호한 미세 캡슐이 된다는 것을 확인하였다. 상기한 소재로 제조된 미세 캡슐의 캡슐화율은 25-45.5% 정도의 범위로 전체적으로 미세캡슐화율은 낮았다. 향후 CMC, maltodextrin 그리고 키토산 등을 소재로 미세 캡슐화 조건을 좀 더 검색할 필요가 있을 것으로 판단되었다.

• 한국해양바이오학회지
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• 제2권4호
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• pp.257-262
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• 2007

Squid viscera oil was investigated by pretreatment method for enhancing the commercial value. Transeterification was performed to reduce rancidity of the oil, off-flavor was removed by using activated carbon adsorption. Analysis using ATD (Automatic Thermal Desorber) and GC/MG shows the efficacy of off-flavor removement. The rates of Transesterification employing inorganic catalyst and biocatalyst were tested, respectively. With stepwise addition of ethanol, the most efficiency of the reaction was achieved by inorganic catalyst. The efficiency of the reaction was estimated by acid value corresponding to rancidity of reaction product.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.869-879
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• 2012

This study was conducted to investigate the effects of dietary lipid source and level on growth performance, blood parameters, fatty acid composition and flesh quality of sub-adult olive flounder Paralichthys olivaceus. Eight experimental diets were formulated to contain 5% squid liver oil (SLO), 5% linseed oil (LO), 5% soybean oil (SO), a mixture of 1% squid liver oil, 2% linseed oil and 2% soybean oil (MIX), no lipid supplementation with high protein level (LL-HP), 10% squid liver oil (HL-SLO), a mixture of 1% squid liver oil, 4.5% linseed oil and 4.5% soybean oil (HL-VO), and 1% squid liver oil with high starch level (LL-HC), respectively. Two replicate groups of fish (average initial weight of 296 g) were fed the diets for 17 wks. After 5 wks, 11 wks and the end of the feeding trial, five fish from each tank were randomly sampled for analysis of body composition. At the end of the feeding trial, final mean weight of fish fed the LL-HP diet was significantly (p<0.05) higher than that of fish fed the HL-VO diet, but did not differ significantly from those of fish fed the SLO, LO, SO, MIX, HL-SLO and LL-HC diets. Fish fed the LL-HP diet showed significantly higher feed efficiency than fish fed the LO, HL-SLO and HL-VO diets. Feed efficiency of fish fed the LO, SO and MIX diets were similar to those of fish fed the SLO and HL-SLO diets. Fish fed the HL-SLO diet showed significantly higher total cholesterol content in plasma compared with other diets. Fatty acid composition of tissues was reflected by dietary fatty acid composition. The highest linoleic (LA) and linolenic acid (LNA) contents in the dorsal muscle were observed in fish fed the SO and LO diets, respectively, regardless of feeding period. The highest eicosapentaenoic acid (EPA) content in the dorsal muscle was observed in fish fed the LL-HP and LL-HC diets after 11 and 17 weeks of feeding, respectively. Fish fed the SLO and HL-SLO diets showed higher docosahexaenoic acid (DHA) content than that of other treatments after 11 and 17 weeks of feeding, respectively. Dietary inclusion of vegetable oils reduced n-3 HUFA contents in the dorsal muscle and liver of fish. The n-3 HUFA contents in tissues of fish fed the SLO and HL-SLO diets were higher than those of fish fed other diets, except for the LL-HP and LL-HC diets. Hardness, gel strength, chewiness and cohesiveness values of dorsal muscle in fish were significantly affected by dietary lipid source. The results of this study indicate that fish oil in fish meal based diets for sub-adult olive flounder could be replaced by soybean oil and linseed oil without negative effects on growth and feed utilization.