• Journal of Bio-Environment Control
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• v.9 no.4
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• pp.230-236
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• 2000

For extension of storability, onion bulbs produced in Cheju Island were exposed to ${\gamma}$-ray irradiation at a dosage of 0, 3, or 6Krad and stored in a natural cavern, or in low (2$^{\circ}C$) or ambient temperature storage condition. Sprouting began from approximately five months in storage, regardless of storage condition, and increased as the storage period increased. From early August, sprouting as high as 50% occurred in the natural storage cavern, and 20% in ambient temperature storage when bulbs were treated with 0 krad ${\gamma}$-ray. However, bulbs treated with 3 or 6krad ${\gamma}$-ray did not any sprouting until six months in storage. No significant weight loss was observed in all treatments until sprouting occurred from 40 days after the initiation of storage. Weight loss, however, increased remarkably after 40 days in storage, indicating that it was closely related to sprouting during storage. Bulbs stored at 2$^{\circ}C$ showed 5~10% rot, but were still edible. Percent rot reached to as high as 50% in bulbs unirradiated and stored at ambient temperatures after seven months, while it in irradiated bulbs reached to 5, 33, and 30% at 2$^{\circ}C$, in a natural cavern, and ambient temperatures, respectively. Sugar contents slightly increased in irradiated bulbs in the later storage period under ambient temperatures, while sugar contents in irradiated bulbs in the later storage period under ambient temperatures, while sugar contents in Allium vegetables generally decrease in later storage if unirradiated. Sulfur content slightly increased in irradiated bulbs in the early storage period and decreased gradually in all treatments in the later storage stage.

• KSBB Journal
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• v.21 no.4
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• pp.292-297
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• 2006

One of the steps in angiogenesis is the degradation of the underlying basement membrane via proteases. Endothelial cells release proteinases to degrade the extracellular matrix for their sprouting in vivo. In this study, we examined the effect of water extracts of Phellinus linteusis(Phellinus extracts) and combination of Phellinus extracts and fibroblast growth factor(FGF-2) on cultured porcine pulmonary artery endothelial cells(PPAECs). Phellinus extracts induced sprouting of PPAECs, which was inhibited by MMPs and plasmin inhibitors, and induced the secretion of matrix metalloproteinase-3(MMP-3) and plasmin. At high concentration of Phellinus extracts($200{\sim}400{\mu}g/mL$), the active MMP-2 secretion was induced. It is therefore, suggested that Phellinus extracts induces the sprouting of cultured endothelial cells by means of increased active MMP-2 and plasmin secretion. Also, combination with Phellinus extracts and FGF-2 produced an enhanced effect on sprouting and secretion of active MMP-2, and MMP-3 and plasmin from PPAECs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.91-97
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• 1970

It is one of the important subjects in fall crop production of potatoes in korea to find the proper direct planting method as a substitute for transplanting method by sprouting inducement at seed-bed. From this point the author has undertaken this experiments to examine the effects of several handling methods of seed tuber pieces at planting on sprouting of fall grown potatoes at Ansung Agricultural College from August to september in 1969. The results of experiments induced sprouting with Gibberellin treatment using the variety Irish Cobbler were as follows; 1. Sun light exposure treatment to cut surface of potato seed pieces resulted in rotting of seed pieces and delay of sprouting and bud growth. Exposure more than three hours induced severe rotting(more than fifty percent) of seed pieces (Table I). 2. When soil moisture are abundant and the duration of sun light. exposure is short, even direct planting method of treated seed pieces with Gibberellin resulted in good potato stands (Table 2). 3. The direct planting method, as the rotting of seed pieces are avoidable are applicable in fall crop production of potatoes us a substitute for transplanting method because the sprouting and bud growth are promoted more in case of direct planting than transplanting (Table 3). 4. When the humidity in the bottle was low no healing effects was found by holding the seed pieces in large closed bottle under the trees for three days before planting (Table 2). 5. If duration of sunlight exposure is short, no difference of potato stands between clear day planting and cloudy day plant ing (Table 3). 6. As long as soil moisture deficiency. was not induced the irrigation at planting shows no effects to protect the seed piece rotting after planting (Table 3).

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.43-49
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• 1985

This study was conducted to investigate the existance of dormancy in newly collected tubers of Eleocharis kuroguwai Ohwi from paddy fields and to determine the effect of various growth regulators on the bud sprouting and tuberization of E. kuroguwai. The maximum percent sprouting of E. kuroguwai collected at every month during winter was less than 60% regardless of varied collection tunes until 50 days after incubation, suggesting the presence of dormancy in E. kuroguwai tubers. This dormancy was markedly broken as the time went by, probably due to the exposure of tubers the extremly low temperature. The treatment of $BA10^{-3}M$ and $BA10^{-3}+GA10^{-6}M$ increased bud sprouting about 10 and 11 times, respectively, than that of the untreated control indicating that BA seems to be one of the most effective agents among the growth regulators used on the bud sprouting of E. kuroguwai. Foliar application of BA increased tuberization of E. kuroguwai by an average of 34.4% as compared with the untreated control. The highest increase, 39.8% was obtained with BA treatment at $10^{-5}M$.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.43-45
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• 2003

This study was carried out to investigate sprouting and cold hardiness during wintering and thawing Periods in 7-year-old mulberry(Morus) three varieties of Kaeryangppong, Shinilppong and Yongcheonppong. The killing points of the mulberry twigs were investigated using DTA(Differential Thermal Analysis). (omitted)

• Korean Journal of Agricultural Science
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• v.43 no.5
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• pp.723-733
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• 2016

Soybeans [Glycine max (L.) Merill] are a rich source of antioxidants and other phytonutrients. Soybean sprouts contain many biologically active secondary metabolites and are rich in polyphenols, flavonoids, and phenolic compounds. In the present study, two soybean cultivars, Hosim, with high oleic acid (- 80% in total seed oil), and Pungsannamul, with normal oleic acid (- 23%) in seed, were examined for changes in the content of crude protein, crude oil, total flavonoids, total phenolics, and DPPH (1,1-diphenyl-2-picryl-hydrazyl) during the sprouting duration of 5 days. The protein content in both the varieties was found to increase by the days of sprouting. The crude oil content of Pungsannamul sprouts was found to be maximum on day 1 (16.9%, w/w) and decreased thereafter to reach to the level of 14.8% on day 5. No significant differences in the crude oil content of Hosim sprouts from day 1 to 5 were observed. Flavonoid content was found to increase up to day 4 and then dropped on day 5, in both the cultivars. Total polyphenol content showed a tendency to increase up to day 3 and started to decrease significantly from day 4. DPPH activity was found to increase up to day 5 in both the varieties. All the components studied in the high oleic acid soybean sprouts showed a change in content during the sprouting process similar to the change that would occur in normal oleic acid soybeans. The study showed that the contents of antioxidant, flavonoid, and polyphenol significantly increase during the sprouting.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.5
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• pp.695-699
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• 2003

Traditional soybean sprouting bucket has some problems which are putrefaction and growing inhibition by the high temperature and carbon dioxide in the bucket during culturing. To solve this problems we developed the new soybean sprouting bucket. The new bucket consisted a square shaped bottom which has 5 draining holes (each 10 mm in dia, 2 mm in height) and four side wall which has two rectangular shaped holes (10 cm long, 0.5 cm wide), and the support vessel which 592 tiny draining holes whose center attached a pipe with 36 small holes (each 2 mm in dia). The new one showed lower temperature and carbon dioxide content during culturing, and the putrefaction was lower, whereas growing degree was higher during culturing at 25$\pm$1$^{\circ}C$ than the traditional bucket.

• Journal of Forest and Environmental Science
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• v.30 no.2
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• pp.218-225
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• 2014

Studies were carried out to standardize and develop a suitable macro-propagation technology for large scale production of superior clonal stock through stem cuttings in Commiphora wightii Arnott (Bhandari), a data deficient medicinal plant of arid region. For the purpose, three experiments were conducted. The first experiment was tried to elucidate the impact of various cutting diameters (0.50-0.75 cm, 0.75-1.00 cm, 1.00-1.50 cm, and >1.50 cm) in combination with varying growing conditions (sunlight, shade house and mist chamber) on shoot sprouting and rooting without using exogenous plant growth regulators. Cutting diameter (size 0.75-1.00 cm) in mist chamber has shown maximum sprouting (90.00%) and rooting (73.33%), primary root (6.67) and secondary root (16.67) followed by 1.00-1.51 cm in mist chamber. Minimum sprouting (40.00%), rooting (33.33%), number of shoot (1.33), primary root (1.00) and number of secondary root (1.00) was recorded in cutting diameter (size >1.50 cm) in sunlight. Second experiment was performed to find out optimum growth regulator concentration of rooting hormone (100, 200, 500 and 1000 ppm) of Indole-3-acetic acid (IAA) and Indole-3-butyric Acid (IBA) on adventitious root formation on cuttings diameter (size 0.25-0.50 cm) in comparison to control. Maximum rooting percentage (93.33%) was recorded in 200 ppm followed by 500 ppm (86.66%) of IBA as compared to control, which showed only 60 per cent sprouting. Third experiment was performed with newly formed juvenile micro-cuttings treated with varying concentrations of IAA and IBA. The juvenile cuttings (size 6-10 cm, basal dia <0.25 cm) were selected as micro-cuttings. The cuttings treated with IBA (500 ppm) showed 64.30% rooting as compared to other treatments. Results of above experiments indicate that cuttings (size 0.75-1.00 cm dia) may be developed in mist chamber for better performance. While using heavier cuttings, no growth promoting hormones is required however; growth regulator 200 ppm concentration of IBA rooting hormone was observed optimum for promoting macro-propagation in stem cuttings of lower diameter class (0.25-0.50 cm).

• Korean Journal of Plant Resources
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• v.13 no.3
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• pp.208-212
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• 2000

The effect of heat covering materials during cultivation of Shigyoku grape was examined on the effect of protection from freezing damage, labor reduction, and disease resistance during the wintering. This experimentwas accomplished using different combination of heat covering materials from the end of Nov as follows: straw and heat conservation cover B, kilding, color fabric A, and white needle punching fabric. Capacity of heat conservation was the least form white needle punching fabric, and the differences of other materials was slight. Early sprouting occurred by the treatment with heat conservation cover B, and the rate of sprouting was about 70.7% after 9 days of sprouting, showing 2.3-12.5% increase. Growth effect was not differ between heat conservation materials. Disease and insects occurred by the in dice 3.0-3.2 of crown gall from the heat conservation cover B treatment. Other damages were not observed or very little from other treatments. Demand of labor during steps of these treatments was more observed by 6% for kilding than heat conservation cover B, and those of color fabric A and white needle punching fabric were reduced by 6 and 15%, respectively.

• BMB Reports
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• v.57 no.2
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• pp.79-85
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• 2024

Pre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T₁ seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiological properties, and biochemical properties of target genes in the ERF1-hdr line were investigated for three years. The ERF1-hdr line showed significantly enhanced seed dormancy and pre-harvest sprouting resistance. qRT-PCR analysis suggested that enhanced ABA signaling resulted in a stronger phenotype of PHS resistance. These results indicate that efficient HDR can be achieved through SNP/InDel replacement using a single and modular configuration applicable to different rice targets and other crops. This work demonstrates the potential to replace all genes with elite alleles within one generation and greatly expands our ability to improve agriculturally important traits.