• 제목/요약/키워드: split peaks

검색결과 40건 처리시간 0.02초

Application study of PCR additives to improve the split peaks in direct PCR

  • Kim, Joo-Young;Kim, Da-Hye;Park, Hyun-Chul;Jung, Ju Yeon;Jin, Gang-Nam;Hwang, In-Kwan;Kang, Pil-Won
    • 분석과학
    • /
    • 제32권4호
    • /
    • pp.155-162
    • /
    • 2019
  • Analysis techniques using DNA profiling are widely used in various fields including forensic science and new technologies such as the Direct PCR amplification method are being developed continuously in order to acquire the DNA profiles efficiently. However, it has a limits such as non-specific amplification according to the quality of crime scene evidence samples. Especially, split peaks caused by excessive DNA samples are one of the important factors that could cause the debate to allow researchers to interpret the DNA profile results. In this study, we confirmed the occurrence rate of split peaks in each STR (short tandem repeats) locus of the $GlobalFiler^{TM}$ kit and investigated the possibility of improving the split peaks using several PCR additives such as DMSO (dimethylsulfoxide), $MgCl_2$, Betaine and Tween-20. As a result, we could make three groups according to the occurrence rate of split peaks in Direct PCR and it was confirmed that the ratio of split peaks could be reduced by DMSO (87.4 %), $MgCl_2$ (84.5 %) and Betaine (86.1 %), respectively. These results indicate that PCR additives such as DMSO, $MgCl_2$ and Betaine can be improve the split peaks in Direct PCR and thereby facilitate subsequently a successful DNA profile results.

Co 불순물을 첨가한 HgGa2S2 단결정의 광흡수 특성

  • 김남오;김형곤;김병철;민완기;전형석;신석두;방태환
    • 대한전기학회:학술대회논문집
    • /
    • 대한전기학회 2004년도 학술대회 논문집 전문대학교육위원
    • /
    • pp.55-57
    • /
    • 2004
  • $HgGa_{2}S_{4}Co^{2+}$ single crystal were grown by the chemical transport reaction(CTR) method. In the optical absorption spectrum of the $HgGa_{2}S_{4}Co^{2+}$ single crystal measured at 298K, three groups of impurity optical absorption peaks consisting of three peaks, respectively, were observed at 673nm, 734nm, and 760nm, 1621nm, 1654nm, and 1734nm, and 2544nm, 2650nm, and 2678nm. At 10K, the three peaks(673nm, 734nm, and 760nm) of the first group were split to be twelve peaks. These impurity optical absorption peaks are assigned to be due to the electronic transitions between the split energy levels of $Co^{2+}$ sited in the $S_4$ symmetry point.

  • PDF

$HgGa_2S_4:CO^{2+}$ 단결정의 불순물 광흡수 (Impurity optical absorption of $HgGa_2S_4:CO^{2+}$ single crystals)

  • 김형곤;김남오;김병철;최영일;김덕태;현승철;방태환;이경섭;구할본
    • 한국전기전자재료학회:학술대회논문집
    • /
    • 한국전기전자재료학회 2004년도 춘계학술대회 논문집
    • /
    • pp.3-7
    • /
    • 2004
  • $HgGa_2S_4:CO^{2+}$ single crystal were grown by the chemical transport reaction(CTR) method. 1n the optical absorption spectrum of the $HgGa_2S_4:CO^{2+}$ single crystal measured at 298K, three groups of impurity optical absorption peaks consisting of three peaks, respectively, were observed at 673nm, 734nm, and 760nm, 1621nm, 1654nm, and 1734nm, and 2544nm, 2650nm, and 2678nm. At 10K, the three peaks(673nm, 734nm, and 760nm) of the first group were split to be twelve peaks. These impurity optical absorption peaks are assigned to be due to the electronic transitions between the split energy levels of $Co^{2+}$ sited in the $S_4$ symmetry point.

  • PDF

HgGa2S4:Co2+ 단결정에서 Co2+ 이온에 의한 광흡수 특성에 관한 연구 (Impurity Optical Absorption of Co2+ Ion in HgGa2S4:Co2+ Single Crystals)

  • 이상열;강종욱
    • 한국전기전자재료학회논문지
    • /
    • 제16권7호
    • /
    • pp.579-583
    • /
    • 2003
  • HgGa$_2$S$_4$: Co$^{2+}$ single crystal were grown by the chemical transport reaction(CTR) method. In the optical absorption spectrum of the HgGa$_2$S$_4$: Co$^{2+}$ single crystal measured at 298K, three groups of impurity optical absorption peaks consisting of three peaks, respectively, were observed at 673nm, 734nm, and 760nm, 1621nm, 1654nm, and 1734nm, and 2544nm, 2650nm, and 2678nm. At 10K, the three peaks(673nm, 734nm, and 760nm) of the first group were split to be twelve peaks. These impurity optical absolution peaks are assigned to be due to the electronic transitions between the split energy levels of Co$^{2+}$ sited in the S$_4$ symmetry point.

광섬유 센서를 이용한 복합재료 고압탱크 변형률 측정 (Strain monitoring of the composite high pressure tanks using the FBG sensors)

  • 박재성;윤종훈;공철원;장영순;이원복;노태호
    • 한국복합재료학회:학술대회논문집
    • /
    • 한국복합재료학회 2003년도 추계학술발표대회 논문집
    • /
    • pp.141-145
    • /
    • 2003
  • The FBG sensors are inserted on the liners of the filament wound pressure tanks. The strains near the welding region of the liners are monitored in the hydro-pressurizing tests. The hydro-pressurizing tests consist of the proof tests at 4500 or 3300 psi and repeated test at the operating pressure, 3000 psi. The FBG sensors work well under $3000\mu\varepsilon$, but the strains calculated from the reflected signals are instable at the high strain level. The transverse compression on the sensor head results in the split of the reflected peaks, and the calculating algorism from the split peaks is not robust under the various signal condition. The FBG sensors fracture near $7500\mu\varepsilon$ level and lose their function permanently.

  • PDF

정상 위 조직과 위암 조직의 시험관 내 수소자기공명분광 (Ex Vivo ${1}^H$ MR Spectroscopy: Normal gastric and cancer tissue)

  • 조지연;신운재;최기승;김수현;은충기;양영일;이정희;문치웅
    • Journal of Gastric Cancer
    • /
    • 제3권3호
    • /
    • pp.151-157
    • /
    • 2003
  • Purpose: In this study, we attempted to ascertain the proton magnetic resonance spectroscopy (${1}^H$ MRS) peak characteristics of human gastric tissue layers and finally to use the metabolic peaks of MRS to distinguish between normal and abnormal gastric specimens. Materials and Methods: Ex-vivo ${1}^H$ MRS examinations of thirty-five gastric specimens were performed to distinguish abnormal gastric tissues invaded by carcinoma cells from normal stomach-wall tissues. High-resolution 400-MHz (9.4-T) ${1}^H$ nuclear magnetic resonance (NMR) spectra of two gastric layers, a proper muscle layer, and a composite mucosasubmucosa layer were compared with those of clinical 64- MHz (1.5-T) MR spectra. Three-dimensional spoiled gradient recalled (SPGR) images were used to determine the size and the position of a voxel for MRS data collection. Results: For normal gastric tissue layers, the metabolite peaks of 400-MHz ${1}^H$ MRS were primarily found to be as follows: lipids at 0.9 ppm and 1.3 ppm; alanine at 1.58 ppm; N-acetyl neuraminic acid (sialic acid) at 2.03 ppm; and glutathione at 2.25 ppm in common. The broad and featureless featureless spectral peaks of the 64-MHz MRS were bunched near 0.9, 1.3, and 2.0, and 2.2 ppm in human specimens without respect to layers. In a specimen (Borrmmann type III) with a tubular adenocarcinoma, the resonance peaks were measured at 1.26, 1.36 and 3.22 ppm. All the peak intensities of the spectrum of the normal gastric tissue were reduced, but for gastric tumor tissue layers, the lactate peak split into 1.26 and 1.39 ppm, and the peak intensity of choline at 3.21 ppm was increased. Conclusion: We found that decreasing lipids, an increasing lactate peak that split into two peaks, 1.26 ppm and 1.36 ppm, and an increasing choline peak at 3.22 ppm were markers of tumor invasion into the gastric tissue layers. This study implies that MR spectroscopy can be a useful diagnostic tool for gastric cancer.

  • PDF

Modified Oxalate Process에 의한 (Ba,Pb)$TiO_3$ 분말합성 및 특성에 관한 연구 (A Study on the Synthesis and Properties of (Ba,Pb)$TiO_3$Powder by Modified Oxalate Process)

  • 최병현;이미재;박선미
    • 한국세라믹학회지
    • /
    • 제33권7호
    • /
    • pp.743-754
    • /
    • 1996
  • In this study (Ba1-xPbx)TiO3 was synthesized by modified oxalate process in order to prevent vaporization of PbO through low temperature synthesis climinate Cl ion reproducibly substitute Pb for by and uniformly distribute ion (Ba1-xPbx)TiO3 was synthesized by coprecipitation of lead acetate barium acetate and ammonium titanyl oxalate have been used as starting materials. The substitution of Pb for Ba was reproducibly possible synthetic temperature of perovskite structure becomes lowed as the Pb concentration increases and fine partic-les (specific surface are :7.2 cm2/g) were obtained, BaTiO3 powders calcined at 90$0^{\circ}C$ for 3 hours were cubic from in XRD analysis and as Pb content was increases evident split of tetragonal peaks could be observed The optimum conditions to synthesize (Ba,Pb)TiO3 powder are the followings ; synthesis temperature (5$^{\circ}C$)

  • PDF

Syntactic 패턴인식에 의한 심전도 피이크 검출에 관한 연구 (Peak Detection using Syntactic Pattern Recognition in the ECG signal)

  • 신건수;김용만;윤형로;이웅구;이명호
    • 대한의용생체공학회:학술대회논문집
    • /
    • 대한의용생체공학회 1989년도 춘계학술대회
    • /
    • pp.19-22
    • /
    • 1989
  • This paper represents a syntactic peak detection algorithm which detects peaks in the ECG signal. In the algorithm, the input waveform is linearly approximated by "split-and-merge" method, and then each line segment is symbolized with primitive set. The peeks in the symbolized input waveform are recognized by the finite-state automata, which the deterministic finite-state language is parsed by. This proposed algorithm correctly detects peaks in a normal ECG signal as well as in the abnormal ECG signal such as tachycardia and the contaminated signal with noise.

  • PDF

Quantitative and Qualitative Differences according to the Shoe Type for the Grand Jete Landing in Ballet

  • Yi, Kyung-Ok;Park, Hye-Rhee
    • 한국운동역학회지
    • /
    • 제21권1호
    • /
    • pp.25-29
    • /
    • 2011
  • The purpose of this study was to analyze quantitative and qualitative differences according to shoe type for the grand jete landing in ballet. The subjects for this study were 9 female ballet majors with an average of 12 years of experience. Subjects jumped, performing a front split, and landed on 1 foot, a movement called the grand jete. Analysis was performed on the students' landing. Independent variables were 3 shoe types: split sole, traditional out sole, and 5-toed forefoot shoes, with bare feet as a control group. Dependent variables were vertical passive ground reaction force and qualitative elements. Passive ground reaction force variables(maximum passive peak value, number of passive peaks, passive force-time integral, and center of pressure) were measured by the Kistler 9281B Force Platform. Qualitative elements were comfort, cushioning, pain, and fit. Statistical analysis included both 1-way ANOVA and Tukey's test for follow-up. Finalized data demonstrated that the 5-toed forefoot shoe allows the forefoot to expand and the toes to individually press down upon landing, increasing foot contact with the surface. Five-toed forefoot shoes minimize passive peaks and pain, while increasing comfort, cushioning, and fit. Most ballet movements are composed of jumping, balancing, landing, and spinning. Wearing 5-toed forefoot shoes allows for a natural range of movement in each toe, to improve both technique and balance. Pain and injuries from ballet can be minimized by wearing the correct shoe type. According to this analysis, it is possible to customized ballet shoes to increase the efficiency of techniques and movements.

Theoretical Explanation of the Peak Splitting of Tobacco-Specific N-Nitrosamines in HPLC

  • Jiang, Juxing;Li, Liangchun;Wang, Mingfeng;Xia, Jianjun;Wang, Wenyuan;Xie, Xiaoguang
    • Bulletin of the Korean Chemical Society
    • /
    • 제33권5호
    • /
    • pp.1722-1728
    • /
    • 2012
  • During the analyzing processes of the compounds, some researchers are puzzled by the analytical signals for some TSNAs (with or without splitting peaks at various pHs and temperatures). In this work, a detailed theoretical study of structural and thermal properties of the $E/Z$ isomers of TSNAs and the corresponding protonated structures was performed using DFT methods. The calculations showed that the $E$ isomers are almost stable than $Z$ isomers, while the $Z$ isomers would be more stable when in protonation. The calculated results predicted the possibility of separation of their $E$ and $Z$ isomer forms and also showed that protonation affects the dipole moment of molecules significantly (0.1-0.5 to 0.7-2.1 Debye). The calculations agreed well with the experiments that the split-up of the HPLC signal for TSNAs into two peaks are very sensitive to the pH and temperature of the mobile-phase.