• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.5
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• pp.135-142
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• 2022

In this study, lap spliced ultra-high strength reinforced concrete beams were tested and the code criteria for calculating the lap splice length which was affected by the transverse reinforcement and concrete covering performance were reviewed. The main variables for test were set as fiber volume fraction and transverse reinforcing bar arrangement to improve the confining performance of the concrete cover. The change of the confining performance of concrete cover according to the increase in the fiber mixing amount at 1% and 2% volume ratio was examined, and D10 stirrups with a spacing of 100 mm were placed in the lap spliced region. As a result of the test, the specimens confined by the stirrups showed a sudden drop of load bearing capacity with horizontal cracking at the position of tensile longitudinal reinforcement. However, horizontal cracks were not appeared at the location of longitudinal reinforcement for the specimens with steel fiber. And these specimens showed gradual decrease of load bearing capacity after experiencing peak load. In particular, it was found that the strain at the position of the tensile longitudinal reinforcements of the specimens to which the mixing ratio of 2% was applied exceeds the yield strain. As a result of measuring the strain on the concrete surface, it was found that the fiber was more effective in preventing damage to the concrete surface than the stirrups for short lap spliced region.

• Biomedical Science Letters
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• v.15 no.4
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• pp.301-307
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• 2009

Whole-body insulin resistance results largely from impaired insulin-stimulated glucose disposal in skeletal muscle. Our previous studies using differential display and quantitative real-time RT-PCR have shown that a novel cDNA band (DD23) had a higher level of expression in insulin resistant skeletal muscle and it was correlated with whole-body insulin action, independent of age, sex, and percent body fat. In this study, we cloned and characterized DD23. The DD23 sequence is part of the 3'UTR region of the RNA binding motif, single stranded interacting protein (RBMS3). We have cloned the full length cDNA for RBMS3 and identified two splice variants. These variants named DD23-L and DD23-S have 15 and 14 exons respectively and differ from RBMS3 in the 3'UTR significantly. Northern blot analyses showed that an ~8.8 kb mRNA transcript of DD23 was predominantly expressed in skeletal muscle and to a lesser extent in placenta, but not in heart, brain, lung, liver, or kidney, unlike RBMS3. Elevated expression levels of these novel alternatively spliced variants of RBMS3 in skeletal muscle may play a role in whole body insulin resistance.

• BMB Reports
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• v.45 no.1
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• pp.32-37
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• 2012

Alternative splicing generates several interleukin-6 (IL-6) isoforms; for them an antagonistic activity to the wild-type IL-6 has been proposed. In this study we quantified the relative abundance of IL-6 mRNA isoforms in a panel of mouse tissues and in C2C12 cells during myoblast differentiation or after treatment with the $Ca^{2+}$ ionophore A23187, the AMP-mimetic AICAR and TNF-${\alpha}$. The two mouse IL-6 isoforms identified, IL-6${\delta}$5 (deletion of the first 58 bp of exon 5) and IL-6${\delta}$3 (lacking exon 3), were not conserved in rat and human, did not exhibit tissue specific regulation, were expressed at low levels and their abundance closely correlated to that of full-length IL-6. Species-specific features of the IL-6 sequence, such as the presence of competitive 3' acceptor site in exon 5 and insertion of retrotransposable elements in intron 3, could explain the production of IL-6${\delta}$5 and IL-6${\delta}$3. Our results argued against biological significance for mouse IL-6 isoforms.

• Molecules and Cells
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• v.23 no.2
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• pp.228-238
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• 2007

Since molecular structure of hnRNP is not available in foreseeable future, it is best to construct a working model for hnRNP structure. A geometric problem, assembly of $700{\pm}20$ nucleotides with 48 proteins, is visualized by a frame work in which all the proteins participate in primary binding, followed by secondary, tertiary and quaternary binding with neighboring proteins without additional import. Thus, 40S hnRNP contains crown-like secondary structure (48 stemloops) and appearance of 6 petal (octamers) rose-like architectures. The proteins are wrapped by RNA. Co-transcriptional folding for RNP fibril of FMR1 gene can produce 2,571 stem-loops with frequency of 1 stem-loop/15.3 nucleotides and 53 40S hnRNP beaded structure. By spliceosome driven reactions, there occurs removal of 16 separate lariated RNPs, joining 17 separate beaded exonic structures and anchoring EJC on each exon junction. Skipping exon 12 has 5'GU, 3'AG and very compact folding pattern with frequency of 1 stem-loop per 12 nucleotides in short exon length (63 nucleotides). 5' end of exon 12 contains SS (Splicing Silencer) element of UAGGU. In exons 10, 15 and 17 where both regular and alternative splice sites exist, SS (hnRNP A1 binding site) is observed at the regular splicing site. End products are mature FMR-1 mRNP, 4 species of Pri-microRNAs derived from introns 7,9,15 and 3'UTR of exon17, respectively. There may also be some other regulatory RNAs containing ALU/Line elements as well.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.5
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• pp.82-90
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• 2018

The nuclear structures are composed of large diameter bars over No.36. If the hooked bars are used for anchorage of large diameter bars, too long length of the tail extension of the hook plus bend create congestion and make an element difficult to construct. To address those problems, headed bars were developed. Provisions of ACI 318-08 specify the development length of headed bars and ignore the effect of transverse reinforcement based on the background researches. However, if headed bars are used at the cut-off or lap splice, longitudinal reinforcements, which are deformed in flexural members, induce tensile stress in cover concrete and increase the tensile force in the transverse reinforcement. The object of this research is to evaluate the effects of transverse reinforcement on the anchorage capacity of headed bar so anchorage test with variable of transverse rebar spacing was conducted. Specimens, which can consider the behavior at the cut-off, were tested. Test results show that failure of specimen without transverse reinforcement was sudden and brittle with concrete cover lifted and developed stress of headed bars was less than half of yield strength of headed bars. On the other hand, in the specimen with transverse reinforcement, transverse rebar directly resist the load of free-end so capacity of specimens highly increased.

• Land and Housing Review
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• v.2 no.1
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• pp.61-68
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• 2011

In order to clarify the structural performances of Welded Deformed Steel Bar Mats (WDSBM), the research stated includes the tests for standard hook of top bars of slab in concrete slab-wall joints, the tests for embedment length of top bar of slab, and the development strength tests for standard hook. The test results are as follows; (1) For slab-wall joints using WDSBM as reinforcement in slab, if the top bars of WDSBM are spliced by ordinary bars with sufficient development length and size, it is enough for the strength and crack control. (2) When WDSBM of slab is spliced in joint, the strength is increased with the embedment of bars of this WDSBM into wall. Beyond peak strength, however, ductility is diminished to that as no splice due to pull-out failure. (3) For slab-wall system, ultimate strain of concrete for flexural compression zone in lower surface of slab seems much greater than that of normal concrete beam. The reason is that normal concrete beam has the joint with $180^{\circ}$, however slab-wall joint has the $90^{\circ}$ of which concrete can be confined.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.27 no.4
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• pp.289-296
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• 2014

The rebar detailing is an important work influencing the final performance and quality of RC structures. But it is one of the most irrational and illogical activity in construction site. Many groups of workers, including main contractors, structural engineers, shop drawers, rebar fabricators, and etc., participate in this activity. A loosely-organized process for this activity is apt to produce a big amount of rebar loss or even degraded structures. A 3-dimensional rebar auto-placing system, called as Rebar Hub, has been designed and implemented in this research. Rebar Hub provides a totally integrated service from 3D structural modeling of buildings to rebar auto-placing considering anchorage, splice, and the length of ordered rebar. In addition, Rebar Hub can recognize the 2D drawing CAD files and then build 3D structural models which are used for the start point of 3D rebar auto-placing. After rebar auto-placing, each members of the 3D structural model have rebar information belonging to them. It means that the rebar information can be used for the afterward works such as quantity-survey, manufacturing and fabrication of rebars. Rebar Hub is showing outstanding performance while applying to practical projects. It has almost five times productivity and reduces the rebar loss up to 3~8% of the initially-surveyed amount of rebar.

• Journal of Bio-Environment Control
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• v.23 no.4
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• pp.383-390
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• 2014

This study was conducted to investigate the growth characteristics of cucumber scion and pumpkin rootstock under different levels of light intensity (photosynthetic photon flux, PPF) and plug cell size in a closed transplant production system with artificial lighting. Cucumber scion and pumpkin rootstock seedlings were grown under the combinations of three levels of PPF (PPF 165, 248, and $313{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and five types of plug tray (50, 72, 105, 128, and 200 cells in the tray) for nine days. The shoot dry weight and relative growth rate increased with increasing PPF and plug cell size. As PPF increased, cucumber scion and pumpkin rootstock seedlings had higher dry matter, lower specific leaf area, and lower hypocotyl length. The first true leaf of cucumber scion and pumpkin rootstock unfolded at eight and seven days after sowing, respectively, except the treatment using 200-cell plug tray. The unfolding of first true leaf of seedlings grown in 200-cell plug tray was delayed by one day. Accordingly, it was considered that the use of small cell size such as 200-cell plug tray would require more time for the production of scion and rootstock. Based on the results, we suggest that cucumber scion and pumpkin rootstock be grown in 105-cell to 128-cell plug tray for eight days and 72-cell to 105-cell plug tray for seven days, respectively, when using splice grafting method with root-removed rootstock. Additionally, higher PPF is suggested to improve the growth and quality of scion and rootstock.