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http://dx.doi.org/10.5483/BMBRep.2012.45.1.32

The expression analysis of mouse interleukin-6 splice variants argued against their biological relevance  

Annibalini, Giosue (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Guescini, Michele (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Agostini, Deborah (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
De Matteis, Rita (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Sestili, Piero (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Tibollo, Pasquale (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Mantuano, Michela (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Martinelli, Chiara (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Stocchi, Vilberto (Dipartimento di Scienze Biomolecolari, Universita degli Studi di Urbino "Carlo Bo")
Publication Information
BMB Reports / v.45, no.1, 2012 , pp. 32-37 More about this Journal
Abstract
Alternative splicing generates several interleukin-6 (IL-6) isoforms; for them an antagonistic activity to the wild-type IL-6 has been proposed. In this study we quantified the relative abundance of IL-6 mRNA isoforms in a panel of mouse tissues and in C2C12 cells during myoblast differentiation or after treatment with the $Ca^{2+}$ ionophore A23187, the AMP-mimetic AICAR and TNF-${\alpha}$. The two mouse IL-6 isoforms identified, IL-6${\delta}$5 (deletion of the first 58 bp of exon 5) and IL-6${\delta}$3 (lacking exon 3), were not conserved in rat and human, did not exhibit tissue specific regulation, were expressed at low levels and their abundance closely correlated to that of full-length IL-6. Species-specific features of the IL-6 sequence, such as the presence of competitive 3' acceptor site in exon 5 and insertion of retrotransposable elements in intron 3, could explain the production of IL-6${\delta}$5 and IL-6${\delta}$3. Our results argued against biological significance for mouse IL-6 isoforms.
Keywords
Alternative splicing; C2C12; Cytokines; Retrotransposons; Splicing error;
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