• Reproductive and Developmental Biology
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• 제31권1호
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• pp.1-6
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• 2007

NOD/SCID 마우스는 선천성 면역결핍을 지닌 마우스로서 이종 세포 및 조직 이식을 위한 실험동물로서 가장 많이 활용되고 있다. 본 연구는 돼지의 골수조직에서 채취한 조혈줄기세포를 면역결핍마우스의 정맥 주입을 통하여 생체 내 주입을 실시한 결과, 마우스의 조혈조직에서 대단히 높은 돼지 T면역세포의 증식이 관찰되었다. 유세포 분석기를 이용해 돼지 골수 조혈세포 생체 이식 6주의 마우스에서의 돼지 T면역세포의 증식과 분화 특성을 분석한 결과, 마우스 조혈조직인 골수($5.4{\pm}1.9%$), 비장($15.4{\pm}7.3%$), 간($21.3{\pm}1.4%$), 림프절($33.5{\pm}32.8%$)에서 돼지 조혈줄기세포 유래 T 세포의 증식과 분화가 관찰되었고, 돼지 helper T 세포와 cytotoxic T 세포의 발달도 확인되었다. 또한 조직 면역염색을 통하여 마우스의 비장조직에 이식한 돼지 면역세포의 중식을 관찰하였다. 본 연구는 NOD/SCID 마우스를 이용해 돼지 조혈줄기세포로부터 T 면역세포로의 분화 및 발달과정을 생체 내에서 분석할 수 있는 유용한 동물모델로서 이용할 수 있음을 보여준다.

• 대한한의학원전학회지
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• 제20권2호
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• pp.175-193
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• 2007

A theory of the school which attach great importance to moxibustion therapy was more developed from 'Ge Hong(葛洪)', 'Wang Tao(王燾)' up to "BianQueXinShu(扁鵲心書)" of 'Dou Cai(竇材)' in Song Dynasty. The first volume of "BianQueXinShu" was described about the principles of health preserving method, diagnosis, treatment related with meridian system, support Yang theory, moxibustion therapy over the 10 chapters and in the continued 3 chapters, explained the symptoms and related moxibustion therapies. The summary is as follows. The Yang energy is the essence of the human body and it is minutely explained in "Hwangdineijing(黃帝內經)". However, the younger scholars after 'Zhang Zhongjing(張仲景)' held different views with "Hwangdineijing" so they didn't control serious diseases. Supporting the Yang energy, it will be possible to human body in good health and long life and perennial youth and longevity. To do like this, the first important thing is a moxibustion, the second is a Taoist hermit medication(丹藥) and the third is well usage of Radix Aconiti Lateralis Preparata. According to the sequence of Yang energy deficiency, the stages of diseases are classified as Ordinary Gi(平氣), Latter deficient state(微虛), The more deficient state(甚虛), Exhausting state(將脫), Exhausted state(已脫) and in the consideration of each stage, it is used gradually with warm-natured berbs, warm-acrid herbs, warm-heated herbs and moxibustion therapy. If it comes to the stage of Exhausted state, the Yang energy is too weak to treat a disease. Therefore it is easy to harm human body with usage the treatment of the Purgationist school theory or the Cold and cool medical school theory, so it is needed a great attention to use these therapies. To summarized the keynote of 'Du Jae''s moxibustion therapy, the one is a minority of selection of points(1$^{\sim}$2 acupuncture points), the second is a majority of moxibustion units(50$^{\sim}$500 units), and the other is a focused selection of points on spleen and kidney(especially Gwanwon, CC4). And in this book, it was explained concretely about the size of moxibustion, according to the experiment with mentioned size, the burning time of moxibustion was almost 4 min 40 sec, so the big size moxibustion was one of the characteristic of moxibustion therapy revealed in this book. Also it was used 'Suseongsan(睡聖散)' - a kind of analgesic herb complexes - to reduce a pain during the usage of moxibustion therapy in this book. To develop the moxibustion theory, it is more investigated in the future that there wasn't significant relation between Gwanwon(CC4) and spleen and kidney meridian in theory, compared to many used Gwanwon(CC4) in the prescription, where as mentioned the importance of spleen and kidney in treatment, that considering the burning time(1 unit - 4 min 40 sec, 12 units an hour, maximum 288 units a day) there were no guidances about meals, sleeping, stool and urine, and that there was no concrete study about the toxicity of 'Suseongsan' as analgesic moxibustion therapy.

• 동의생리병리학회지
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• 제21권1호
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• pp.308-317
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• 2007

This study was investigated to know the correlation of complete blood count(CBC), biochemical examination and DSOM fluents(Diagnosis System of Oriental Medicine, (C)2005-01-122-004154). There are 5 fluents in DSOM such as DSOM score(病機點數), mean of the index for pathogenic factor(病機指標 平均), 5-division of DSOM score(病機點數 五點尺度), 5-division of the index for pathogenic factor(病機點數 五點尺度), weighted pathogenic factor(病機加重値).We have carried out clinical trials who volunteered for Sasang constitutional medicine and Oriental OB & GY, Oriental Medical hospital of Dong-Eui University, from May 2005 to June 2006. Volunteers were 245 persons. Because 7 persons didn't checkup DSOM, so we analyzed the results statistically for 238 persons. In the relation of CBC and DSOM, the scores(病機點數, zp) and mean of the index for Pathogenic factor(病機指標 平均, zps) showed correlation more frequently, and correlation with results of RBC, hemoglobin, hematocrit was more significant. Correlation with fluents of pathogenic factor(病機) were more significant and high in deficiency of blood(血虛), insufficiency of Yang(陽虛), coldness(寒), damp(濕), dryness(燥), kidney(腎), phlegm(痰), heat syndrom(熱), lung(肺), and was very low in spleen(脾). There was no correlation with deficiency of Yin(陰虛). If volunteers have DSOM fluents, results of RBC and RBC index was decreased(- derection), and results of RDW, ESR was increased(+ direction). But increase and decrease direction in heat syndrom(熱), lung(肺) was contrary to the others. Correlation with fluents of Pathogenic factor(病機) of WBC, platlet, PDW, MPV was not many. In the relation of biochemical examination and DSOM fluents, correlation with results of albumin, uric acid, triglyceride was more significant. If volunteers have fluents of pathogenic factor(病機), result of examination was usually decreased. Especially result of examination was decreased(- derection) highly in deficiency of blood(血虛), stagnation of (氣滯) coldness(寒), dryness(燥), and was increased(+ direction) highly in heat syndrom(熱), lung(肺). But there was a tendency to show high correlation with specific pathogenic factor (病機) and specific examination in biochemical examination.

• 동의신경정신과학회지
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• 제27권3호
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• pp.131-146
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• 2016

Objectives: This study was performed to determine the correlation between Instrument on Pattern Identification for Depression and Psychological tests by Pearson Correlation Coefficient and Regression analysis.Methods: Two assessors carried out the evaluation using the instrument on pattern identification for depression. They also performed the following psychological tests: Beck Depression Inventory (BDI), State-Trait Anxiety Inventory (STAI), State-Trait Anger Expression Inventory (STAXI), the Temperament and Character Inventory (TCI), Insomnia Severity Index (ISI_Total), Self-disclosure Inventory, subjective well-being Inventory, Health perception Inventory, and Personality Assessment Inventory (PAI). A total of 167 participants who got HAM-D score over 12 were targeted for the evaluation. Our research team carried out Pearson correlation coefficient analyses and regression analysis between pattern identification for depression and Psychological tests. We listed the results by descending order and interpreted the results.Results: Pearson correlation analysis revealed the following results: 1) Stagnation of Liver Gi was associated with BDI (0.60) and STAI (0.55); 2) Dual Deficiency of the Heart and Spleen was associated with BDI (0.60), ISI_Total (0.52), and STAI (0.42); 3) Relieving stagnation of Phlegm-Gi was associated with BDI (0.65), STAI (0.54), and Subjective well-being inventory (−0.52); 4) Gi-deficiency Mingled with sputum was associated with BDI (0.50), ISI_Total (0.40), and STAI (0.395); 5) Stagnant Gi transforming into fire was associated with BDI (0.56), STAI_TR (0.51), and Health perception Inventory (−0.458); 6) Yin-Deficiency with Effulgent Fire was associated with BDI (0.55), ISI_total (0.54), and STAI (0.41).Conclusions: Through correlation analysis between Instrument on Pattern Identification for Depression and Psychological tests, we could suggest a System for Oriental Medical Diagnosis for Depression.

• 한국식품영양과학회지
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• 제43권9호
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• pp.1317-1324
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• 2014

본 연구에서는 울금 20% 주정 추출물이 LP-BM5 MuLV에 감염된 MAIDS 모델에서 면역조절 효과를 확인하기 위해 cytokines 생산, 혈장 면역글로불린 농도, T 세포 및 B 세포의 증식능, NK 세포의 활성능을 측정하였다. 그 결과 LPBM5 MuLV 감염으로 인하여 감소된 T 세포 및 B 세포의 증식능, NK 세포의 활성능을 울금 20% 주정 추출물 식이 투여가 증가시켰으며, Th1 type cytokines(IL-2, IFN-${\gamma}$)의 생성량을 증가시키고 Th2 type cytokines(IL-4, IL-10)은 억제시킴으로써 Th1/Th2 type cytokine 발현을 조절하여 면역 항상성을 유지하는 효과를 보였다. 따라서 울금은 면역조절 효과를 가진 천연 기능성 소재로서의 가능성을 기대할 수 있다.

• 동의신경정신과학회지
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• 제12궈1호
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• pp.81-95
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• 2001

Though, these days there are increasing many patient of insomnia, there was not considered literatual study on insomnia. So, the result of consideration about cause and process of insomnia from thirty kinds of literatures, are as follows. 1. Early literature like Huangjaenankyung(黃帝內經), Sanghanron(傷寒論) reffered to insomnia as accompanying symptom. on the other hand, Myung(明), Chung(淸)'s literatures reffered to that as chief symptom or distinguished chief symptom from accompanying symptom. 2. There were tendency of deductive expanding of various masters and tendency of induction of simple theory that was based on Huangjaenankyung(黃帝內經). 3. Huangjaenankyung(黃帝內經) showed basic process of the sleep disorder that 'exhausting of Yin and excess of Yang (陰盡陽盛)', protecting energy does not invade Yin portion(陽氣不入於陰). And Huangjaenankyung(黃帝內經) showed cause of insomnia that deficiency of vital energy and blood, imbalanced of spleen and stomach, a fever as a invasion in the outside, lung system's disease. This became a basic cause and process of the sleep disorder in ancient period. 4. Sanghanron(傷寒論) occurred to insomnia as accompanying symptom in progress, remedy of a fever invaded outside, Kumkyeyoriak showed as origination in weakness, fatique, various diseases. Out of that, there is a significance in description of insomnia from some disease like histery or neurosis. 5. Jaebyungwonhuron(諸病源候論), Chunkumbang(千金方), Kukbangseo(局方書.) occured to a fire of heart(心慤) and a deficiency of heart and gall bladder(心膽虛) in defails. Insomnia is caused by agony of seven emotion, delivering of a child, are similar to insomnia is caused by psychologic disorders. Injaesanghanyusu(仁劑傷寒類書) occured to exhausting of Yin and excess of Yang (陰盡陽盛), imbalanced of stomach(胃不和) invasion of coldness(傷寒) are brought a conclusion of assumption of sap(津液耗損) brought about unreturn of yin energy. 6. Manbyunghuechum(萬病回春) in Myung period (明代) made much of portion of phlegm's influence about spiritual function. Kyungakjunsu(景岳全書) valued much of treatment divided according to excess and deficiency. Junginmaekchi(證因脈治) occurred to concept of pyorihesil(表裏虛實), Dongyibokam(東醫寶鑑) synthesised various theories. 7. Hyuljungron(血證論), Byunjungkimun(辨證奇聞), Suksilbirok(石室秘錄) made much of surprisemeni(驚恐) Consideration(思慮), liver's dryness(肝燥) is caused by liver's weakness(肝虛), imbalance of haert -kidney(心腎不交), seven emotion(七情). Especially, ftyujungchijae(類證治載) said that heart, liver, gall-bladder, kidney, surprisment, consideration baought to a conclusion of inbalance of Yang and Yin (陽不交陰). There is a tendency in literature mostly that literature showed separation of insomnia as a chief symptom. 8. These days there are increasing many patient of insomnia. So, it is needed to study about insomnia as a psychologic disease. Saying in conclusion, it is needed that we have to recognize in modern style based on ancient style of cause and process of insomnia. It is regarded to study about insomnia definitely and experimently.

• 혜화의학회지
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• 제16권1호
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• pp.139-146
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• 2007

1. The etiological causes of Pain in Shoulder and Arm based on literatures of Oriental medicine are attack of wind-heat on the lung, wind cold, damp-heat struggle between the vital energy and pathogenic factor and six pathogenic factors And all these causes are the conception of blockage syndrome, $Q_1$ and blood stagnating in meridian system. 2. Symptoms of Pain in Shoulder and Arm based on literatures of Oriental medicine are shoulder pain, restriction of activity and radiating pain. 3. The treatments of Pain in Shoulder and Arm based on literatures of Oriental medicine are mainly composed of both medical therapy for $B_1$ syndrome due to pathogenic wind, deficiency of both $Q_1$ and blood, consumption of the liver and the spleen, and also acupuncture and moxibustion treatment by selection of acupoint. And those treatments are for treating etiology. And also there are treatments using the meridian system and Twelve Muscle Region and Ashihyeol for the purpose of treating the symptoms. 4. Acupoints such as Gyun-u, Gyun-jung, Goi-ji, Ju-ryo and Bi-no are most used in treating shoulder and arm pain based on based on literatures of Oriental medicine.

• 동의생리병리학회지
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• 제18권6호
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• pp.1569-1574
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• 2004

A general review is made on Insamyangwi-tang(인삼양위탕). Following conclusions are drawn from the clinical cases of Insamyangwi-tang in Hyungsang medicine. Insamyangwi-tang is composed of four different prescriptions of Huisaeng-san, Sakoonja-tang, Eajin-tang and Pyungwi-san. Huisaeng-tang is usually prescribed for the intestinal convulsion. Sakoonja-tang for the deficiency of Ki. Eajintang for retention of phlegm, Insamyangwi-tang is effective in strengthening the spleen, drying the dampness, warming the middle-warmer to stop vomiting, regulating the flow of Ki, and eliminating phlegm. Insamyangwi-tang is applicable to malaria caused by cold, intestinal convulsion, abdominal mass, edema, tympanites, Yin syndrome of exogenous febrile disease, distension, lack of appetite, stomachache, and diarrhea. Persons with the following characteristic in Hyungsang are more susceptible to Insamyangwi-tang ; Jung type, Hyul type, fish type, Taium meridian type, white fat damp constitution, person with big mouth, and woman rather than man.

• 대한한방소아과학회지
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• 제18권1호
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• pp.93-104
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• 2004

Objectives: Transverse myelitis is a rare neurological disorder that is part of a spectrum of neuroimmunologic diseases of the central nervous system. A patient was hospitalized with intestinal pseudoobstruction(poor oral feeding, vomiting, abdominal pain, constipation) secondary to Transverse myelitis. We treated her with Oriental medical approach and obtained satisfactory result. Methods: The Herbal medicine(Bihwa-eum), Acupuncture, Moxibustion therapy were applied for improving the deficiency of energy of the spleen and stomach, regulate the flow of vital energy. Results: After treatments, Patient's vomiting frequency is decreased and abdominal pain is improved and oral feeding is available. She gains in weight continuously. Conclusion: The authors thought that Bihwa-eum is effective to vomiting and abdominal pain due to intestinal pseudoobstruction. The more study is needed.

• 대한한의학원전학회지
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• 제31권1호
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• pp.127-138
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• 2018

Objectives : The paper reviews the texts related to Naosuxiaoshuo in Huangdineijing, and investigates its cause, mechanism, prognosis and symptom expressions. Methods : The paper searches for the texts within Huangdineijing that deal with Naosuxiaoshuo, and tries to clarify the significance and the characteristics of Naosuxiaoshuo using the annotators comments regarding this issue. Moreover, the paper tries to search for similarities between the symptoms of Naosuxiaoshuo and the relevant diseases in modern medicine. Results : Naosuxiaoshuo is a serious disease where the diminishing of the brain's parenchyma can even lead to death. The cause is yin-deficiency based on the lack of vital essence and body fluid, and it also can be caused by the external pathogen or other stimulations. Moreover, it shows some similarities with brain atrophy and cerebrospinal fluid diseases. Conclusions : Naosuxiaoshuo should be treated with a focus on yin-tonifying dealing with spleen related to production of body fluid and the kidney related to storage of vital essence. It is also important to prevent external pathogens or stimulations damaging the bone marrow.