• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.246-254
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• 2022

Current studies have revealed the capacity of mesenchymal stem cells (MSCs) in term of immunomodulatory properties, and this distinct potential is downgraded according to the disease duration of patients-derived MSCs. In order to enhance the immunomodulatory and anti-tumorigenic properties of the rheumatoid arthritis (RA) joints-derived MSCs, we aggregate synovial fluid-derived MSCs from RA joints (RA-hMSCs) into 3D-spheroids by the use of hanging drop culture method. Cells were isolated from synovial fluids of RA joints with longstanding active status over 13 years. For aggregation of RA-hMSCs into 3D-spheroids, cells were plated in hanging drops in 30 μL of advanced DMEM (ADMEM) containing 25,000-30,000 cells/drop and cultured for 48 h. To analyze the comparative immunomodulatory effects of 3D-spheroid and 2D monolayer cultured RA-hMSCs and then cells were cultured in ADMEM supplemented with 20% of synovial fluids of RA patients for 48 h and were evaluated by qRT-PCR for their expression of mRNA levels of inflammatory and anti-inflammatory markers. Cellular aggregation of RA-hMSCs was observed and cells were aggregate into a single sphere. Following treatment of RA patient's synovial fluids into the RA-hMSCs, spheroids formed RA-hMSCs showed significantly (p < 0.05) higher expression of TNFα stimulated gene/protein 6 (TSG-6) than the monolayer cultured RA-hMSCs. Therefore, the 3D-spheroid culture methods of RA-hMSCs were more effective than 2D monolayer cultures in suppressing inflammatory response treated with 20% of RA-synovial fluids by expression of TNFα (TSG-6) according to the immune response and enhanced secretion of inflammatory factors.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.297-299
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• 2015

In this paper we have examined the linear stability of triangular equilibrium points in the photogravitational restricted three body problem when both primaries are triaxial rigid bodies, the bigger one an oblate spheroid and source of radiation. The orbits about the Lagrangian equilibrium points are important for scientific investigation. A number of space missions have been completed and some are being proposed by various space agencies. We analyze the periodic motion in the neighbourhood of the Lagrangian equilibrium points as a function of the value of the mass parameter. Periodic orbits of an infinitesimal mass in the vicinity of the equilibrium points are studied analytically and numerically. The linear stability of triangular equilibrium points in the photogravitational restricted three body problem with Poynting-Robertson drag when both primaries are oblate spheroids has been examined by A. Kumar (2007). We have found the equations of motion and triangular equilibrium points for our problem. With the help of the characteristic equation we have discussed stability conditions. Finally, triangular equilibrium points are stable in the linear sense. It is further seen that the triangular points have long or short periodic elliptical orbits in the same range of ${\mu}$.

• Proceedings of the Korea Information Processing Society Conference
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• 2004.11a
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• pp.829-832
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• 2004

실제영상으로 가상환경을 구축해서 사용자가 가상환경을 돌아다님으로써 보다 큰 몰입감과 현실감을 제공하는 영상기반 가상현실 기술은 최근 들어 웹 기반 가상현실시스템을 구축하기 위해서 많이 사용된다. 이 기술은 가상환경 구축에 있어 항해를 쉽게 하기 위한 한 방법으로 넓은 시각 영역(field of view)을 얻을 수 있는 wide-angle 렌즈를 흔히 사용한다. 어안렌즈(fish-eye lens)는 전형적인 넓은 시각 영역을 가진 렌즈로서, 매우 큰 radial distortion 을 가진다. 왜곡을 없앤 영상을 얻기 위해 본 논문에서는 구면기하(spherical geometry) 및 사영기하(projective geometry)를 사용하여 어안영상을 보정하는 non-metric기법을 제안한다. 제안한 이 방법은, 기존의 방법들 보다는 쉽고 빠른 속도로 왜곡을 보정할 수 있으므로 어안영상의 왜곡을 보정하는 하드웨어를 효율적으로 구현할 수 있다. 그리고 spheroid 를 이용해 좀 더 왜곡을 정확히 보정방법과 별도의 서보 모터 없이 pan/title 를 가능케 하는 시점이동에 따른 왜곡 보정 방법을 제시한다.

• Journal of Microbiology and Biotechnology
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• v.32 no.9
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• pp.1209-1216
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• 2022

To better understand the effects of PEGylation and biotinylation on the delivery efficiency of proteins, the cationic protein lysozyme (LZ) and anionic protein bovine serum albumin (BSA) were chemically conjugated with poly(ethylene glycol) (PEG) and biotin-PEG to primary amine groups of proteins using N-hydroxysuccinimide reactions. Four types of protein conjugates were successfully prepared: PEGylated LZ (PEG-LZ), PEGylated BSA (PEG-BSA), biotin-PEG-conjugated LZ (Bio-PEG-LZ), and biotin-PEG-conjugated BSA (Bio-PEG-BSA). PEG-LZ and Bio-PEG-LZ exhibited a lower intracellular uptake than that of LZ in A549 human lung cancer cells (in a two-dimensional culture). However, Bio-PEG-BSA showed significantly improved intracellular delivery as compared to that of PEG-BSA and BSA, probably because of favorable interactions with cells via biotin receptors. For A549/fibroblast coculture spheroids, PEG-LZ and PEG-BSA exhibited significantly decreased tissue penetration as compared with that of unmodified proteins. However, Bio-PEG-BSA showed tissue penetration comparable to that of unmodified BSA. In addition, citraconlyated LZ (Cit-LZ) showed reduced spheroid penetration as compared to that of LZ, probably owing to a decrease in protein charge. Taken together, chemical conjugation of targeting ligands-PEG to anionic proteins could be a promising strategy to improve intracellular delivery and in vivo activity, whereas modifications of cationic proteins should be more delicately designed.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.35 no.2
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• pp.131-134
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• 2011

We propose a spheroid chip that uses removable cell trapping barriers and that is capable of forming and extracting multicellular spheroids. By using a conventional well plate and flask, it is difficult to form small-sized spheroids, which resemble avascular 3D cell-cell interaction. It was difficult to extract spheroids using conventional microchips and fixed cell trapping barriers. The proposed chip, however, facilitates both formation and extraction of spheroids by using removable cell trapping barriers formed by membrane deflection. The cell trapping barriers, formed at the membrane pressure of 50 kPa, hold the cells in the trapping region at a cell inlet pressure of 145.155 Pa. After incubation for 24 h, the trapped cells form uniform spheroids. We successfully extract the spheroids at a cell inlet pressure of 5 kPa after removing the membrane pressure. The extracted spheroids have a diameter of $197.2{\pm}11.7Bm$ with a viability of $80.3{\pm}7.7%$. Using the proposed chip, uniform spheroids can be formed and these spheroids can be safely extracted for carrying out the post-processing of spheroids.

• Bulletin of the Korean Space Science Society
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• 1999.09a
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• pp.108-108
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• 1999

No Abstract, See Full Text

• Proceedings of the Korean Nuclear Society Conference
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• 2004.05a
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• pp.88.1-88.1
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• 2004

• Journal of Environmental Science International
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• v.23 no.11
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• pp.1801-1805
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• 2014

Longshore current is main transportation mean causing movement of bed load and suspended particle in coastal waters, and effective measurement method and suitable equipment for shallow water coastal environment where is frequently exposed to atmosphere. Measurement equipment for longshore drift was designed and miniature model was applied to Gyeongpo beach in May and June, 2014. The equipment consists of three main elements, spheroid outer casing, spheroid inner casing, observation module equipped with GPS. Gyroscope principle was applied to observation module, and GPS receiver always can be directed upwards. Miniature models were installed along Gyeonpo beach, and it was well to track the flow of longshore current. This research described the design and function of the equipment and results of field experiments.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.137-144
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• 1994

Spherical multicellular aggregates of adult rat hepatocytes (spheroid) which have tissue like structure, were formed and immobilized in the pores of polyurethane foam (PUF) which was used as a culture substratum. These hepatocyte/spheroids, about 100 $\mu\textrm{m}$ in diameter, have maintained higher differentiated functions than those of hepatocyte/monolayer for about 3 weeks in serum-free medium. Then, we designed a prototype module of an artificial liver support system using a PUF/spheroid packed-bed, in which hepatocyte/spheroids were immobilized at high density. The urea synthesis activity of the artificial liver was maintained at least 10 days in 100% rat blood plasma. We start examining the performance of hybrid artificial liver in an ex vivo extracorporeal experiment with an acute hepatic failure rat.

• Journal of Korea Foundry Society
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• v.9 no.6
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• pp.483-489
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• 1989

In the stable eutectoid transformation of austenite in ductile cast iron, ferrite forms around the graphite spheroid. As carbon diffuses through the ferrite ring, the graphite spheroid must eularge and the ferrite ring must deform plastically to accomodate this growth. A model has been proposed to clarify this mechanism. The alloying effects of molybdenum, nickel and copper were studied in a series of heats cast into a range of casting section sizes. Regression analysis was used to illustrate the effects of alloying and cooling rate on the microstructure of ductile cast iron.