• Title/Summary/Keyword: spectrophotometer

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Assay of Glucose-6-phosphate Dehydrogenase in E. coli Cells Ruptured by Phage Ghost (Phage Ghost로 破裂시킨 E. coli 에서 Glucose-6-phosphate Dehydrogenase의 活性度 測定)

  • Yun, Se-Joong
    • Journal of the Korean Chemical Society
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    • v.12 no.4
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    • pp.142-145
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    • 1968
  • The relative activity of glucose-6-phosphate dehydrogenase in E. coli was measured at 340 $m\mu$ with a spectrophotometer. The synchronized E. coli cells in exponential phase were treated with Phage($T_2$) ghost, and used as a enzyme solution directly. This assay method supposed to be useful for the continuous determination of enzyme activity in E. coli.

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Reconstitution of Membrane Proteins from Corn Seedlings with Phosphatidylcholine (옥수수 유식물 조직에서 분리한 막 단백질과 Phosphatidylcholine의 재조합)

  • 오승은
    • Journal of Plant Biology
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    • v.33 no.4
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    • pp.321-323
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    • 1990
  • Membrane proteins isolated from the coleoptile and mesocotyl tissues of corn seedlings were solubilized with Triton X100 and reconstituted with phosphatidylcholine at 2$0^{\circ}C$. The proteoliposomes were incubated and proton uptake into the vesicles was measured with a spectrophotometer. Addition of ATP to the reaction mixture was found to result in an active accumulation of proton into the vesicles. These results indicate that the preparation contains tightly bound phosphatidylcholine vesicles with reconstituted H+ -ATPase from the plant cell membranes.

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Vertical Distribution of the Heavy Metals Content in Soils (토양중 중금속의 수직분포도 조사)

  • 엄석원;최한영
    • Journal of environmental and Sanitary engineering
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    • v.7 no.1
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    • pp.87-94
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    • 1992
  • In order to investigate the content of heavy metals in soil according to vertical profiles, 72 soil samples were collected from 4 sampling sites : Ulchiro-2 ga, Dugdo, Sungnaedong, and Amsa- dong. The content of mercury was measured by a mercury analyzer and those of lead, copper and zinc were measured by an atomic absorption spectrophotometer. From the results of this study, it was shown that the content of heavy metals decreased gradually from the surface soil to the 2 m- depth soil. But, there was no difference in the contents of the heavy metals in the 3m, the 4m and the 5m-depth soil.

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Bioconversion of Furfural into 2-Furoic acid by Zooglea sp. (Zooglea sp.에 의한 furfural에서 2-furoic acid로의 생물학적 전환)

  • 변규희;한홍의;홍순우;하영칠
    • Korean Journal of Microbiology
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    • v.17 no.4
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    • pp.193-197
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    • 1979
  • Attempts were made to elucidate the process of biodegradation of furfural. Zoogloea sp. isolated from soil could convert furfural into 2-furoic acid by a certain enzyme (s) and also accumulated it extracellularly. This substance was extracted with diethyl ether and identified with U.V. spectrophotometry, high pressure liquid chromatography (HPLC) and IR spectrophotometry.

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Spectrophotometric Determination of N-3-Pyridylmethyl-N'-p-nitrophenyl Urea in Rodenticide (흡광광도법(吸光光度法)에 의(依)한 살서제(殺鼠劑) N-3-Pyridylmethyl-N'-p-nitrophenyl Urea(RH-28787)의 정량(定量))

  • Kim, Bak-Kwang
    • Journal of Pharmaceutical Investigation
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    • v.6 no.2
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    • pp.83-87
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    • 1976
  • Microanalysis of rodenticide by visible absorption method was attempted. The following procedure was found to be very simple and to give accurate results. RH-28787 was added to dimethylformamide (DMF), and extracted after stirring for about 20 minutes in extractor $(30^{\circ}C)$. After extraction, the absorbance of DMF solution, was measured at 346 nm by using spectrophotometer. The result agrees closely with the experimentally determined value by means of the gas chromatograph.

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A Stability on the Solvents of Spectral Sensitizer for Photographic Emulsion (사진유제용 분광증감색소의 용매에 대한 안정성)

  • Kim, Yeoung-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.14 no.2
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    • pp.65-70
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    • 1997
  • The symmetric naphthothiazolo carbocyanine is of industrial importance as red-sensitizing dye in the spectral sensitization of emulsion microcrystals in negative film-making. The stability on the solvents of naphthothiazolo carbocyanine dye was measured by UV-Vis spectrophotometer, and then all of solvents were stabilized sensitizer. The maximum absorption peak range in various solvents was $600nm{\sim}612nm$. But it was identified that only methanol can be used to photographic emulsion.

Isolation and Purification of Antimicrobial Active Substances from Aloe vera peel(II) (Aloe vera peel에서 항균활성 물질의 분리.정제(II))

  • 박정순;신용서;류일환;이갑상
    • The Korean Journal of Food And Nutrition
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    • v.13 no.2
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    • pp.146-151
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    • 2000
  • To evaluate anticaries and antiinflammation of Aloe vera peel, antimicrobial substances were extracted from Aloe vera peel and identified. The antimicrobial active substances of water extract were successfully purified with solvent fractionation, silica gel column chromatography, preparative thin layer chromatography and UV spectrophotometer. Two purified active substances were identified as aloe-emodin and barbaloin by Mass Spectrometer, 1H-NMR and FT-IR.

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Characterization of $ZrO_2$ thin films fabricated by glancing angle deposition

  • Sobahan, K.M.A;Park, Yong-Jun;HwangBo, Chang-Kwon
    • Proceedings of the Optical Society of Korea Conference
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    • 2008.02a
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    • pp.281-282
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    • 2008
  • The glancing angle deposition (GLAD) technique was used to fabricate $ZrO_2$ thin films by electron-beam evaporation. The crystal structure, cross-sectional structure, surface morphology and optical properties are characterized by X-ray diffraction meter (XRD, Rigaku, Cu $K{\alpha}$ - radiation), scanning electron microscope (SEM), and spectrophotometer, respectively.

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Synthesis of 2-(Nitroimino)imidazoline (2-(Nitroimino)imidazoline의 합성)

  • Kim, Yeoung-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.27 no.4
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    • pp.482-486
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    • 2010
  • In this study, 2-(Nitroimino)imidazoline was prepared by the reaction of nitroguanidine with ethylenediamine in the presence of water at $65^{\circ}C$. The product was identified by using various analytical tools such as melting point, elemental analyzer, IR spectrophotometer, mass spectrometer and TLC. This compound can be used as intermediate for prepareing pesticide-imidacloprid packing and cosmetics preservative.

Hydrolysis Reaction of Ti Double Alkoxide (Ti Double Alkoxide의 수화반응)

  • ;W.C. LaCourse
    • Journal of the Korean Ceramic Society
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    • v.27 no.5
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    • pp.693-697
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    • 1990
  • TIACA has two different side groups(ligands) as iso-propoxy groups and acetylacetonate groups. These two side groups have different hydrolysis rates. The two different rates of hydrolysis confer unique properties on TIACA, which are stability in aqueous solution and easy preparation of TiO2 and its multi components gels without precipitation. Their hydrolysis rates were monitored with I.R. and U.V. spectrophotometer.

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