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Paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) of Korean pufferfish

  • Lee, Ka Jeong;Ha, Kwang Soo;Jung, Yeoun Joong;Mok, Jong Soo;Son, Kwang Tae;Lee, Hee Chung;Kim, Ji Hoe
    • Fisheries and Aquatic Sciences
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    • v.24 no.11
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    • pp.360-369
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    • 2021
  • Paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) are neurotoxins that display pharmacological activity that is similar to that of specific sodium channel blockers; they are the principle toxins involved in shellfish and puffer fish poisoning. In Korea, puffer fish is a very popular seafood, and several cases of accidental poisoning by TTX have been reported. Therefore, it is necessary to determine whether puffer fish poisoning incidents are caused by PSTs or by TTX. In this study, we used mouse bioassay (MBA) and liquid chromatograph-tandem mass spectrometry (LC-MS/MS) to determine the presence of PSTs and TTX in puffer fish from an area near Mireuk-do, Tong-Yeong on the southern coast of Korea from January through March, 2014. The toxicity of PSTs and TTX extracts prepared from three organs of each specimen was analyzed by MBA. Most of the extracts killed mice with typical signs of TTX and PSTs. The LC-MS/MS analysis of seven specimens of Takifugu pardalis and Takifugu niphobles, each divided into muscles, intestines, and liver, were examined for TTX. In T. pardalis, the TTX levels were within the range of 1.3-1.6 ㎍/g in the muscles, 18.8-49.8 ㎍/g in the intestines, and 23.3-96.8 ㎍/g in the liver. In T. niphobles, the TTX levels were within the range of 2.0-4.5 ㎍/g in the muscles, 23.9-71.5 ㎍/g in the intestines, and 28.1-114.8 ㎍/g in the liver. Additionally, the toxicity profile of the detected PSTs revealed that dcGTX3 was the major component in T. pardalis and T. niphobles. When PSTs were calculated as saxitoxin equivalents the levels were all less than 0.5 ㎍/g, which is below the permitted maximum standard of 0.8 ㎍/g. These findings indicate that the toxicity of T. pardalis and T. niphobles from the southern coast of Korea is due mainly to TTX and that PSTs do not exert an effect.

An Experimental Study on the Performance of Expandable Steel Pipe Pile (확장형 강관말뚝의 성능에 대한 실험적 연구)

  • Kim, Junghoon;Kim, Uiseok;Kim, Jiyoon;Kang, Minkyu;Choi, Hangseok
    • Journal of the Korean GEO-environmental Society
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    • v.23 no.1
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    • pp.39-49
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    • 2022
  • Expandable steel pipe piles are installed by inserting expansion equipment to increase the cross-sectional area of steel pipes, which can improve the pile performance compared to micro-piles. In this paper, a hydraulic expansion device was developed to expand steel pipe piles in practice. A series of laboratory and field tests were conducted to verify the performance of the developed expansion device to expand steel pipes. The expansion capability and expandable range was evaluated by measuring the strain and expansion time at the maximum pressure of the hydraulic expansion device. The thinner steel pipe, the larger strain but longer expansion time required in the test. For example, the 4.0-mm-thick steel pipe showed strain reduction by 30% and a decrease in the required expansion time by 40% compared to the 2.9-mm-thick steel pipe. In addition, in-situ expansion tests were performed to verify the expandability of steel pipes under the ground, and the exhumed specimen showed clear expanded sections. The structural integrity was determined by comparing the material performance the original and expanded specimens.

Evaluating rheological properties of excavated soil for EPB shield TBM with foam and polymer (폼과 폴리머를 활용한 EPB 쉴드 TBM 굴착토의 유동학적 특성 평가)

  • Byeonghyun Hwang;Minkyu Kang;Kibeom Kwon;Jeonghun Yang;Hangseok Choi
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.25 no.5
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    • pp.387-401
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    • 2023
  • The Earth Pressure Balanced (EPB) Shield Tunnel Boring Machine (TBM) is widely employed for constructing urban underground spaces due to its minimal vibration and low noise levels. The injection of additives offers several advantages, including maintaining shield chamber pressure, reducing shear strength, minimizing cutter wear, and decreasing the permeability of the excavated soil. This technique is known as soil conditioning and involves the application of additives such as foam, polymer, and bentonite slurry. In this study, weathered granite soil commonly encountered at domestic tunnel sites was used as a soil specimen. Foam and polymer were applied as additives to assess the rheological properties of conditioned soils. The workability was evaluated through slump tests, while the rheological properties were assessed through laboratory pressurized vane shear tests conducted under the same conditions. Specially, the polymer was applied under specific conditions with low workability with high slump values, with the aim of evaluating the impact of polymer application. The test results revealed that with an increase in the Foam Injection Ratio (FIR), the slump value also increased, while the torque, peak strength, yield stress, apparent viscosity, and thixotropic area decreased. Conversely, an increase in the Polymer Injection Ratio (PIR) led to results opposite to those of FIR. Additionally, a correlation between the slump value and yield stress was proposed. When comparing conditions with only foam applied to those with both foam and polymer applied, even with similar slump values, the yield stress was found to be lower in the latter conditions.

A Study on the Combustion Characteristics of the Crown of Pine Trees in the Drying Season (건조기 소나무 수관부 부위별 연소특성에 관한 연구)

  • Hyuk Kwon;Jong Ho Lee
    • Journal of the Korean Society of Safety
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    • v.38 no.4
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    • pp.39-46
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    • 2023
  • Pine trees, which account for 23% of the forested area of the Republic of Korea, are highly vulnerable to fire in comparison to broad-leaved trees due to the presence of consistent water tube sections throughout the year and resin that is composed of approximately 20% oil. In addition, the pattern of forest fires is determined by weather, topographic conditions, and fluctuation in moisture content. Therefore, when fire breaks out in pine tree forests during the dry season (January to March), it is difficult to extinguish, and it quickly spreads. In this study, the combustion characteristics of pine needles, pine cones, and pine branches in the water tube sections of living pine trees were compared and analyzed in accordance with the moisture content as per the ISO 5660-1. The monthly moisture content was analyzed from January to March, and it was found to be the lowest in March, with 53.6% for pine needles, 51.9% for pine branches, and 10.9% for pine cones. In particular, pine cones were more vulnerable to fire as compared to pine needles and pine branches because their moisture content was more than five times lower than that of pine needles and branches. The ignition time, which affects the speed of flame propagation, was the most rapid in March, and the fastest ignition time was for pine cones, at 19 seconds, followed by 34 seconds for pine needles, and 256 seconds for pine branches. The pine branches were the last to be ignited due to the effect of density, according to the thickness and specific gravity of the specimen. The peak heat release rate, which is a measurable index of fire intensity, was analyzed for pine cones and found to be 184.28 kW/m2 , while the mean effective heat of combustion was 19.79 MJ/kg, and the total heat release rate was 39.7 MJ/m2 , and these values were higher than those of pine branches and pine needles. Thus, we determined that the flame propagation speed and fire intensity according to the moisture content can be used to evaluate the risk of fire to the water tube section of pine trees. It is suggested that because of the combustion characteristics of the pine cone in March, that is when the forest is most vulnerable to fires.

Structural Static Test for Validation of Structural Integrity of Fuel Pylon under Flight Load Conditions (비행하중조건에서 연료 파일런의 구조 건전성 검증을 위한 구조 정적시험)

  • Kim, Hyun-gi;Kim, Sungchan;Choi, Hyun-kyung;Hong, Seung-ho;Kim, Sang-Hyuck
    • Journal of Aerospace System Engineering
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    • v.16 no.1
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    • pp.97-103
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    • 2022
  • An aircraft component can only be mounted on an aircraft if it has been certified to have a structural robustness under flight load conditions. Among the major components of the aircraft, a pylon is a structure that connects external equipment such as an engine, and external attachments with the main wing of an aircraft and transmits the loads acting on it to the main structure of the aircraft. In civil aircraft, when there is an incident of fire in the engine area, the pylon prevents the fire from spreading to the wings. This study presents the results of structural static tests performed to verify the structural robustness of a fuel pylon used to mount external fuel tank in an aircraft. In the main text, we present the test set-up diagram consisting of test fixture, hydraulic pressure unit, load control system, and data acquisition equipment used in the structure static test of the fuel pylon. In addition, we introduce the software that controls the load actuator, and provide a test profile for each test load condition. As a result of the structural static test, it was found that the load actuator was properly controlled within the allowable error range in each test, and the reliability of the numerical analysis was verified by comparing the numerical analysis results and the strain obtained from the structural test at the main positions of the test specimen. In conclusion, it was proved that the fuel pylon covered in this study has sufficient structural strength for the required load conditions through structural static tests.

Study on the Fiber Alignment using Vacuum Filtration Method (Vacuum Filtration method를 이용한 단섬유(short fiber) 배열 영향성 분석)

  • Sung-Kwon Lee;Moo-Sun Kim;Ho-Yong Lee;Sung-Woong Choi
    • Composites Research
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    • v.36 no.3
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    • pp.162-166
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    • 2023
  • Although composite materials are increasingly utilized in general high-strength structures, the demand of performance characteristics as the multifunctional materials has been increased especially in the area of complex electronic devices. While the heat dissipation properties of devices are typically required properties, control of thermal property of composite material especially in the vertical direction is one of the problems to be solved due to its lamination process. In this study, CFRP was manufactured using the Vacuum filtration method for three types of solvent and CFs. In the composite material manufacturing process, the effect of solvent was examined using three solvents where solvents are most frequently used for the dispersion of fibers. Morphology of fiber was observed through a microscope to confirm the arrangement of CFs in the vertical direction. The alignment of fiber was examined through the measurement of the thermal conductivity of the manufactured specimen. For the thermal conductivity measurement, the higher thermal conductivity was obtained with the lower aspect ratio of CF. For the thermal conductivity in the through-plane direction, 8.687 W/m·K, 10.322 W/m·K, and 13.005 W/m·K of thermal conductivity was measured in the DMF, NMP and Acetone, respectively.

Radioactive Concentrations in Chemical Fertilizers

  • Gwang-Ho Kim;Jae-Hwan Cho
    • Journal of Radiation Protection and Research
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    • v.47 no.4
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    • pp.195-203
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    • 2022
  • Background: The aim of the present study was to determine radioactive concentrations in fertilizers known to contain essential nutrients. Results of this study could be used as basic data to monitor the impact of chemical fertilizers on the environment and public health. Nitrogen fertilizers, calcium fertilizers, sulfur fertilizers, phosphate acid fertilizers, and potassium chloride fertilizers were used in this study. Materials and Methods: Five chemical fertilizers were pulverized, placed in polyethylene containers, and weighed. The time to measure each specimen was set to be 3,600 seconds for a scintillator-based gamma-ray spectroscopy system. Concentration of gamma radionuclide was analyzed based on obtained spectra. At the end of the measurement, the spectrum file was stored and used to calculate radioactive concentrations using a gamma-ray spectrometer software. Results and Discussion: In the nitrogen fertilizer, 3.49 ± 5.71 Bq/kg of 137Cs, 34.43 ± 7.61 Bq/kg of 134Cs, and 569.16 ± 91.15 of 40K were detected whereas 131I was not detected. In the calcium fertilizer, 5.74 ± 4.40 Bq/kg of 137Cs (the highest concentration among all fertilizers), 22.37 ± 5.39 Bq/kg of 134Cs, and 433.67 ± 64.24 Bq/kg of 40K were detected whereas 131I was not detected. In the sulfur fertilizer, 347.31 ± 55.73 Bq/kg of 40K, 19.42 ± 4.53 Bq/kg of 134Cs, 2.21 ± 3.49 of 137Cs, and 0.04 ± 0.22 Bq/Kg of 131I were detected. In the phosphoric acid fertilizer, 70,007.34 ± 844.18 Bq/kg of 40K (the highest concentration among all fertilizers) and 46.07 ± 70.40 Bq/kg of 134Cs were detected whereas neither 137Cs nor 131I was detected. In the potassium chloride fertilizer, 12,827.92 ± 1542.19 Bq/kg of 40K was and 94.76 ± 128.79 Bq/kg of 134Cs were detected whereas neither 137Cs nor 131I was detected. The present study examined inorganic fertilizers produced by a single manufacturer. There might be different results according to the country and area from which fertilizers are imported. Further studies about inorganic fertilizers in more detail are needed to create measures to reduce 40K. Conclusion: Measures are needed to reduce radiation exposure to 40K contained in fertilizers including phosphoric acid and potassium chloride fertilizers.

Concrete Crack Detection Inside Finishing Materials Using Lock-in Thermography (위상 잠금 열화상 기법을 이용한 콘크리트 마감재 내부 균열 검출)

  • Myung-Hun Lee;Ukyong Woo;Hajin Choi;Jong-Chan Kim
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.27 no.6
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    • pp.30-38
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    • 2023
  • As the number of old buildings subject to safety inspection increases, the burden on designated institutions and management entities that are responsible for safety management is increasing. Accordingly, when selecting buildings subject to safety inspection, appropriate safety inspection standards and appropriate technology are essential. The current safety inspection standards for old buildings give low scores when it is difficult to confirm damage such as cracks in structural members due to finishing materials. This causes the evaluation results to be underestimated regardless of the actual safety status of the structure, resulting in an increase in the number of aging buildings subject to safety inspection. Accordingly, this study proposed a thermal imaging technique, a non-destructive and non-contact inspection, to detect cracks inside finishing materials. A concrete specimen was produced to observe cracks inside the finishing material using a thermal imaging camera, and thermal image data was measured by exciting a heat source on the concrete surface and cracked area. As a result of the measurement, it was confirmed that it was possible to observe cracks inside the finishing material with a width of 0.3mm, 0.5mm, and 0.7mm, but it was difficult to determine the cracks due to uneven temperature distribution due to surface peeling and peeling of the wallpaper. Accordingly, as a result of performing data analysis by deriving the amplitude and phase difference of the thermal image data, clear crack measurement was possible for 0.5mm and 0.7mm cracks. Based on this study, we hope to increase the efficiency of field application and analysis through the development of technology using big data-based deep learning in the diagnosis of internal crack damage in finishing materials.

Effects Of Cultured Bone Cell On The Regeneration Of Alveolar Bone (배양골세포 이식이 치조골재생에 미치는 영향)

  • Jeong, Soon-Joon;Herr, Yeek;Park, Joon-Bong;Lee, Man-Sup;Kwon, Young-Hyuk
    • Journal of Periodontal and Implant Science
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    • v.26 no.1
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    • pp.1-26
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    • 1996
  • This study was performed to estimate the effects of cultured bone cell inoculated on porous type hydroxyaptite for the regeneration of the artificial alveolar bone defect. In this experiment 3 beagle dogs were used, and each of them were divided into right and left mandible. Every surgical intervention were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium(30mg/Kg). To reduce the gingival bleeding during surgery, operative site was injected with Lidocaine hydrochloride(l:80,000 Epinephrine) as local anesthesia. After surgery experimental animal were feeded with soft dietl Mighty dog, Frisies Co., U.S.A.) for 1 weeks to avoid irritaion to soft tissue by food. 2 months before surgery both side of mandibular 1st premolar were extracted and bone chips from mandibular body were obtained from all animals. Bone cells were cultured from bone chips obtained from mandible with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Porous type hydroxyapatite were immerse into the high concentrated cell suspension solution, and put 4 hours for attachin the cells on the surface of hydroxyapatite. Graft material were inserted on the artificial bone defect after 3 days of culture. Before insertion of cellinoculated graft material, scanning electronic microscopic observation were performed to confirm the attachment and spreading of cell on the hydroxyapatite surface. 3 artificial bone defects were made with bone trephine drill on the both side of mandible of the experimental animal. First defect was designed without insertion of graft material as negative control, second was filled with porous replamineform hydroxyapatite inoculated with cultured bone marrow cells as expermiental site, and third was filled with graft materials only as positive control. The size of every artificial bone defect was 3mm in diameter and 3mm in depth. After the every surgical intervention of animals, oral hygiene program were performed with 1.0% chlorhexidine digluconate. All of the animals were sacrificed at 2, 4, 6 weeks after surgery. For obtaining histological section, tissus were fixed in 10% Buffered formalin and decalcified with Planko - Rycho Solution for 72hr. Tissue embeding was performed in paraffin and cut parallel to the surface of mandibular body. Section in 8um thickness of tissue was done and stained with Hematoxylin - Eosin. All the specimens were observed under the light microscopy. The following results were obtained : 1. In the case of control site which has no graft material, less inflammatory cell infiltration and rapid new bone forming tendency were revealed compared with experimental groups. But bone surface were observed depression pattern on defect area because of soft tissue invasion into the artificial bone defect during the experimental period. 2. In the porous hydroxyapatite only group, inflammatory cell infiltration was prominet and dense connective tissue were encapsulated around grafted materials. osteoblastic activity in the early stage after surgery was low to compared with grafted with bone cells. 3. In the case of porous hydroxyapatite inoculated with bone cell, less inflammatory cell infiltration and rapid new bone formation activity was revealed than hydroxyapatite only group. Active new bone formation were observed in the early stage of control group. 4. The origin of new bone forming was revealed not from the center of defected area but from the surface of preexisting bony wall on every specimen. 5. In this experiment, osteoclastic cell was not found around grafted materials, and fibrovascular invasion into regions with no noticeable foreign body reaction. Conclusively, the cultured bone cell inoculated onto the porous hydroxyapatite may have an important role of regeneration of artificial bone defects of alveolar bone.

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A Study on the Paleomagnetism of Southern Korea since Permian (페름기(紀) 이후(以後) 한국(韓國)의 고지자기(古地磁氣)에 관(關)한 연구(硏究))

  • Kim, Kwang Ho;Jeong, Bong II
    • Economic and Environmental Geology
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    • v.19 no.1
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    • pp.67-83
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    • 1986
  • Oriented hand samples were collected from Gobangsan Formation and Nogam Formation in the north of Danyang and south of Yeongchun, from Bansong Group in and around Danyang, from Nampo Group in Chungnam Coalfield, from Gyeongsang Supergroup distributed from Waegwan through Daegu to Gyeongsan and from Daegu to Goryong, and from volcanic flows in Jeongog area and Jeju Island to study the paleomagnetism of southern Korea since Permian. Stepwise alternating field and thermal demagnetization experiments were carried out to determine optimum fields and temperatures. Observed mean paleomagnetic directions are as follows: $D=331.5^{\circ}$, $I=25.1^{\circ}$, $a95=12.8^{\circ}$ for Permian, $D=325.6^{\circ}$, $I=46.1^{\circ}$, $a95=11.8^{\circ}$ for Triassic, $D=313.4^{\circ}$, $I=43.1^{\circ}$, $a95=16.0^{\circ}$ for early Jurassic, $D=41.3^{\circ}$, $I=64.6^{\circ}$, $a95=4.5^{\circ}$ for early Cretaceous, $D=28.3^{\circ}$, $I=58.1^{\circ}$, $a95=2.3^{\circ}$ for late Cretaceous, $D=2.0^{\circ}$, $I=55.8^{\circ}$, $a95=6.6^{\circ}$for Quaternary. To describe the tectonic translocation of southern Korean block, northern Eurasian continental block was used as a reference frame. For each age since Permian the expected northern Eurasian field directions in terms of paleolatitude and declination were calculated. The paleolatitudes of Permian ($13.2^{\circ}N$) and early Jurassic ($25.1^{\circ}N$) obtained from the study area are quite different from those of Permian ($66.0^{\circ}N$) and early Jurassic ($68.1^{\circ}N$) which are expected for northern Eurasia. The declinations of Permian ($331.5^{\circ}$) and early Jurassic ($313.4^{\circ}$) are also quite different from those of the Permian ($56.6^{\circ}$) and the early Jurassic ($47.5^{\circ}$) expected for northern Eurasia. The Cretaceous paleolatitude is similar to the expected within error limit, but the declination for the same period is significantly different from that of the expected for the northern Eurasia. From the above evidences it is suggested that the south Korean land mass had moved from low latitude in Permian to north and sutured to northern continental block since early Jurassic. The relative rotations of early Cretaceous($27.4^{\circ}$) and late Cretaceous($10.8^{\circ}$) to northern Eurasian continent reveal that the Korean land mass might be rotated clockwise in two different times, probably in late Early Cretaceous and in Tertiary.

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