• Journal of Microbiology
• /
• v.45 no.5
• /
• pp.453-459
• /
• 2007

We developed a multiplex PCR (mPCR) assay to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Corynebacterium spp. and seudomona aeruginosa. This method employs a single tube and multiple specific primers which yield 200, 281, 346, 423, 542, and 1,427 bp PCR products, respectively. All the PCR products were easily detected by agarose gel electrophoresis and were sequenced to confirm the specificity of the reactions. To test this method, DNA extracted from urine samples was collected from 96 sexually transmitted disease or prostatitis patients at a local hospital clinical center, and were subjected to the mPCR assay. The resulting amplicons were cloned and sequenced to exactly match the sequences of known pathogenic isolates. N. gonorrhoeae and Corynebacterium spp. were the most frequently observed pathogens found in the STDs and prostatitis patients, respectively. Unexpectedly, P. aeruginosa was also detected in some of the STD and prostatitis samples. More than one pathogen species was found in 10% and 80.7% of STD and prostatitis samples, respectively, indicating that STD and prostatitis patients may have other undiagnosed and associates. The sensitivity of the assay was determined by sing purified DNA from six pathogenic laboratory strains and revealed that this technique could detect pathogenic DNA at concentrations ranging from 0.018 to $1.899\;pg/{\mu}l$. Moreover, the specificities of this assay were found to be highly efficient. Thus, this mPCR assay may be useful for the rapid diagnosis of causative infectious STDs and prostatitis. useful for the infectious STDs and prostatitis.

• Journal of Korean Society of Archives and Records Management
• /
• v.17 no.4
• /
• pp.109-126
• /
• 2017

This study deals with the theoretical analysis of important audiovisual archives, specifically regarding the historical context of the given theme. The study will feature a historical review of the concept, which is the basis for audiovisual archive research, followed by the specificities of audiovisual archives in sharp contrast to public archives, libraries, and museums. Audiovisual archives will also be covered primarily regarding documentaries as well as places and institutions. The study will conclude with various issues of audiovisual archives in today's digital age. The study hopes to contribute to the growth of audiovisual archive research in Korea and the establishment of various audiovisual archives that will be addressed on a national level both in policy and in practice.

• Journal of the Economic Geographical Society of Korea
• /
• v.13 no.4
• /
• pp.601-622
• /
• 2010

The purpose of this paper is to analyze the value chain reorganization of cultural contents industry cluster in Daegu city and its impact on the regional economy. To achieve this goal, this research examined the change of value added structure, institutional milieu and networks among regional innovators and their effect on value chain reorganization of cluster. The result shows that the scope of the value chain generating value added have been extended and the networks among regional innovators have been increased. Such value chain reorganization has a positive effect on the regional economy. In the context of public policy, however, the focus of industrial policies needs to generate cultural and institutional specificities in a given region and to strength Triple helix innovation system.

• Korean journal of applied entomology
• /
• v.31 no.4
• /
• pp.509-515
• /
• 1992

Phytophagous insects were surveyed from field populations of Compositae weeds in the order Campanulales. Forty five insect species including unidentified ones of 43 genera in 5 orders and 17 families were recorded from 16 species in 12 genera of Compositae weeds. The hemipterans and coleopterans were the most abundant insects representing 13 and 13 species, repectively. Aphids and leaf beetles were important phytophagous insects on Compositae weeds. The potential biological control insects were Uromelan gobonis and Aethes cnicana on Cirsium japonicum var. ussuriensis and Cryptosiphum artemisiae on Artemisia lavandulaefolia. The leaf beetles, Chrysolina aurichalcea, Longitarsus succineus, Basilepta fulvipes, and Hamushia konishii were often collected from A. lavandulaefolia. Aphids and leaf beetles showed host specificities. The roles of hemipterans collected from the flowers of A. lavandulaefolia and Erigeron canadensis were questionable.

• KSBB Journal
• /
• v.19 no.6 s.89
• /
• pp.489-493
• /
• 2004

The hydrolysis characteristics of various fatty acids composing the fish oil was investigated for function of acyl chain specificities using Lipase-OF 360,000 from Candida cylindracea. The hydrolysis of fatty acids decreased with the increase of the number of carbon and double bond in the fatty acids, in case that the number of double bond and the position of the first double bond from the methyl group of fatty acids were the same. The position of the first double bond was found to be an acyl chain specificity of Lipase-OF 360,000 for the hydrolysis of fish oil. Lipase-OF 360,000 also showed the another acyl chain specificity that the increase of double bond of fatty acids, having the same number of carbon and the position of double bond, brought about the decrease of hydrolysis.

• Journal of the Korean BIBLIA Society for library and Information Science
• /
• v.31 no.1
• /
• pp.163-192
• /
• 2020

Old maps of Dokdo are records with the physical specificities of a map while also holding historical meanings and evidencial values as ancient writings. Therefore more detail and meaningful descriptions are needed than other general records. However, in comparison to the high interest in these maps, there is a lack of systematic and integrative descriptions. Hence, this study aimed to propose the description elements of the old maps of Dokdo holding evidential values. For this purpose, first, literature reviews and the characteristics of the old maps of Dokdo were analyzed. Next, for determining description elements using institutions that possess old maps of Dokdo, case studies were conducted in 12 domestic and foreign institutions and compared the rules of ISAD(G). The final description elements were suggested by reflecting on the evaluation of five experts in old maps. Fifty description elements in a total of 8 areas - identity areas, context areas, content and structure areas, conditions of access and use areas, allied materials areas, note areas, description control areas, and other areas were proposed.

• Journal of the Korean Society of Safety
• /
• v.28 no.1
• /
• pp.95-101
• /
• 2013

This study tried to develop a basis for quantitative index of working postures associated with WMSDs(Work-related Musculoskeletal Disorders) that could overcome realistic restriction during application of typical checklists for WMSDs evaluation. The baseline data for this study was obtained from automobile manufacturing company(A total of 603 jobs were observed). Specifically, data for shoulder postures was analyzed to have a better and more objective method in terms of job relevance than typical methods such as OWAS, RULA, and REBA. Major statistical tools were Clustering, Logistic regression and so on. The main results in this study could be summarized as follows; 1) The relationships between working postures and WMSDs symptoms at shoulder were statistically significant based on the results from logistic regression. 2) Based on clustering analysis, three levels for WMSDs risk at shoulder were produced for both flexion and abduction were statistically significant. Specific results were as follows; Shoulder flexion: low risk(< $37.7^{\circ}$), medium risk($37.7^{\circ}{\sim}70.0^{\circ}$), high risk(> $70.0^{\circ}$) Shoulder abduction: low risk(< $26.5^{\circ}$), medium risk($26.5^{\circ}{\sim}56.8^{\circ}$), high risk(> $56.8^{\circ}$). 3) The sensitivities on risk levels of shoulder flexion and abduction were 64.0% and 20.6% respectively while the specificities on risk levels of shoulder flexion and abduction were 99.1% and 99.3% respectively. The results showed that the data associated with shoulder postures in this study could provide a good basis for job evaluation of WMSDs at shoulder. Specifically, this evaluation methodology was different from the methods usually used at WMSDs study since it tried to be based on direct job relevance from real working situation. Further evaluation for other body parts as well as shoulder would provide more stability and reliability in WMSDs evaluation study.

• Applied Biological Chemistry
• /
• v.28 no.3
• /
• pp.149-155
• /
• 1985

590 strains of Rhizobia were isolated from root nodules of the legumes collected at 223 sites in Korea. According to their host specificities they were classified into R. japonicum(218 strains), R. phaseoli(101 strains), R. trifolii(97 strains), R. meliloti(4 strains), R. leguminosarium(1 strain), Rhizobium species(101 strains), and unidentified species(159 strains). 3 potent strains R-138, R-168, and R-214 of R. japonicum have been selected based on the infectivity to soybean cultivar and effeciency of nitrogen fixation. It was observed that the fast-growing strains of R. japonicum contained 1 to 4 plasmids of M.W. of 35-300 Md. However, plasmids were hardly detected for the slow-growing strains.

• Research in Plant Disease
• /
• v.21 no.2
• /
• pp.103-106
• /
• 2015

Isolates of the rice blast fungus show a range of tissue-specificities infecting leaves, nodes, neck and panicles. Although neck and panicle blast cause significantly greater yield losses than the leaf blast, virulence tests of the blast isolates have been performed only rice leaves instead of neck and panicles. In this study, we have developed a virulence test method for neck and panicle blast. We selected three representative isolates from each of leaf, neck, and panicle blast. We observed that severe disease lesions developed on the neck and the panicles when the infected rice plants were incubated in a dew chamber for 48 h instead of 24 h when tested on leaves. Unlike the leaf blast, a typical lesion on the neck and panicles appeared after 14 days post-infection as opposed to 7 days with leaf blast. This method will be applied to examine tissue-specificity of the rice blast fungus isolates.

• Archives of Plastic Surgery
• /
• v.37 no.4
• /
• pp.346-350
• /
• 2010

Purpose: Diagnosis of diabetic foot infection is sometimes difficult, since the classical inflammatory signs and leukocytosis may be absent due to the decreased host immune response in diabetics. Therefore inflammatory blood markers, such as white blood cell (WBC) count, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) have been commonly needed to confirm the diagnosis of infection. The purpose of this study is to evaluate the diagnostic usefulness of WBC, ESR and CRP for detection of diabetic foot infection. Methods: Peripheral blood samples were taken from 113 patients with diabetic foot ulcers admitted from June 2007 to April 2009. Diabetic foot infection was diagnosed according to the microbiological culture from soft tissue and bone specimens. Reference values of tests were 4500-11000 /${\mu}L$ for WBC count, 0-20 mm/hr for ESR, and 0-5 mg/L for $CRP^{13,14}$. Sensitivities, specificities, positive and negative predictive values of laboratory tests were calculated and analysed. Receiver-operator characteristic (ROC) curve was also created. Results: There was a significant difference in WBC, ESR, and CRP between infectious group and noninfectious group (p<0.05). The sensitivity of WBC>11,000 /${\mu}L$ ESR > 20 mm/hr, and CRP > 5 mg/L was 30%, 96%, and 84%. The specificity was 86%, 14%, and 50% for WBC, ESR, and CRP, respectively. Positive predictive value was 88%, 78%, and 84%, and negative predictive value was 28%, 50%, and 50% respectively. The areas under the ROC curve for WBC, ESR and CRP were 0.72, 0.75, and 0.78 respectively. Conclusion: Based on the results of this study, we conclude that CRP is more useful method in predicting and diagnosing infection than WBC, ESR in diabetic foot ulcer patients.