• Proceedings of the Botanical Society of Korea Conference
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• 2002.04a
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• pp.73-82
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• 2002

The committing step in Met and S-adenosyl-L-methionine (SAM) synthesis is catalyzed by cystathionine ${\gamma}$ -synthase (CGS). Transgenic Arabidopsis thaliana overexpressing CGS under control of 35S promoter show increased soluble Met and its metabolite S-methylmethionine, but only at specific stages of development. CGS-overexpressing seedlings are resistant to ethionine. Similar results were obtained with transgenic potato plants overexpressing Arabidopsis CGS. Several of the transgenic lines show silencing of CGS resulting in deformed p]ants with a reduced capacity for reproductive growth similar as transgenic plants by antisense RNA (CGS[-]). Exogenous feeding of Met to the CGS[-] and CGS[+] silenced plants partially restores their growth. Similar morphological deformities are observed in plants cosuppressed for SAM synthetase, even though such plants accumulate 250 fold more soluble Met than wild type and they overexpress CGS. The results suggest that the abnormalities associated with CGS and SAM synthetase silencing are due in part to a reduced ability to produce SAM, and that SAM may be a regulator of CGS expression.

• Asian Journal of Turfgrass Science
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• v.18 no.4
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• pp.211-219
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• 2004

Transgenic zoysiagrass (Zoysia japonica cv. Zenith) plants have been obtained by particle bombardment of embryogenic callus with the plasmid pSMABuba, which contains hygromycin resistance (hpt) and bialaphos resistance (bar) genes. Parameters on DNA delivery efficiency of the particle bombardment were partially optimized using transient expression assay of a chimeric $\beta-glucuronidase$(gusA) gene driven by the CaMV 35S promoter. Stably transfarmed zoysiagrass plants were recovered with a selection scheme using hygromycin. Transgenic zoysiagrass plants were confirmed by PCR analysis with specific primer for bar gene. Expression of the transgene in transformed zoysiagrass plants was demonstrated by Reverse transcriptase (RT)-PCR analysis. All the tested transgenic plants showed herbicide BastaR resistance at the field application rate of $0.1\%-0.3\%$.

• Korean Journal of Environmental Biology
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• v.28 no.3
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• pp.158-171
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• 2010

The flora of Deokjeok Island was investigated 35 times from September 2007 to September 2008. Based on voucher specimens, the study indicated that this area consisted of 535 taxa in total; 108 families, 320 genera, 480 species, 2 subspecies, 45 varieties and 8 forms were present. Compositae and Graminae were the first and second most common families surveyed in the region, respectively. Among the more remarkable examples found were four taxa of Korean endemic plants, six taxa of rare and endangered plants designated by National Arboretum, and 55 taxa of specific plants that have been designated by the Ministry of Environment. Furthermore, 50 taxa of naturalized plants were observed and they occupied 17.9% of the total in the surveyed area. Although some parts of the investigated region were disturbed by human impact, the ecological status was comparatively well preserved. However, the area where the remarkable plants were discovered should be more systematically protected. Since the proportion of naturalized plants in the human habitation area was considerably high and the spreading of these plants seems consistent, it is expected that the ecosystem of this area may become critically unstable in near future.

• Molecules and Cells
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• v.28 no.5
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• pp.463-472
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• 2009

Although the possible cellular roles of several ubiquitin-specific proteases (UBPs) were identified in Arabidopsis, almost nothing is known about UBP homologs in rice, a monocot model plant. In this report, we searched the rice genome database (http://signal.salk.edu/cgi-bin/RiceGE) and identified 21 putative UBP family members (OsUBPs) in the rice genome. These OsUBP genes each contain a ubiquitin carboxyl-terminal hydrolase (UCH) domain with highly conserved Cys and His boxes and were subdivided into 9 groups based on their sequence identities and domain structures. RT-PCR analysis indicated that rice OsUBP genes are expressed at varying degrees in different rice tissues. We isolated a full-length cDNA clone for OsUBP6, which possesses not only a UCH domain, but also an N-terminal ubiquitin motif. Bacterially expressed OsUBP6 was capable of dismantling K48-linked tetra-ubiquitin chains in vitro. Quantitative real-time RT-PCR indicated that OsUBP6 is constitutively expressed in different tissues of rice plants. An in vivo targeting experiment showed that OsUBP6 is predominantly localized to the nucleus in onion epidermal cells. We also examined how knock-out of OsUBP6 affects developmental growth of rice plants. Although homozygous T3 osubp6 T-DNA insertion mutant seedlings displayed slower growth relative to wild type seedlings, mature mutant plants appeared to be normal. These results raise the possibility that loss of OsUBP6 is functionally compensated for by an as-yet unknown OsUBP homolog during later stages of development in rice plants.

• The Plant Pathology Journal
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• v.38 no.3
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• pp.229-238
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• 2022

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) is the most serious soil-borne disease in the world and has become the main limiting factor of watermelon production. Reliable and quick detection and quantification of Fon are essential in the early stages of infection for control of watermelon Fusarium wilt. Traditional detection and identification tests are laborious and cannot efficiently quantify Fon isolates. In this work, a real-time polymerase chain reaction (PCR) assay has been described to accurately identify and quantify Fon in watermelon plants and soil. The FONRT-18 specific primer set which was designed based on identified specific sequence amplified a specific 172 bp band from Fon and no amplification from the other formae speciales of Fusarium oxysporum tested. The detection limits with primers were 1.26 pg/µl genomic DNA of Fon, 0.2 pg/ng total plant DNA in inoculated plant, and 50 conidia/g soil. The PCR assay could also evaluate the relationships between the disease index and Fon DNA quantity in watermelon plants and soil. The assay was further used to estimate the Fon content in soil after disinfection with CaCN₂. The real-time PCR method is rapid, accurate and reliable for monitoring and quantification analysis of Fon in watermelon plants and soil. It can be applied to the study of disease diagnosis, plant-pathogen interactions, and effective management.

• Journal of architectural history
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• v.26 no.6
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• pp.29-40
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• 2017

Soswaewon as a typical villa garden of Korea is a symbolic garden that its diverse compositions imply a specific meaning. This study reviewed the purpose of introduction, meaning, and location of the landscape plants as one of the compositions and covering quite a large part of Soswaewon through 48 Yeong (meaning 48 poems of Soswaewon) and Soswaewondo (meaning Map of Soswaewon). In 48 Yeong, 27 verses describe landscape plants as their key poetic matters. The most frequently mentioned top 3 plants are bamboos, pine trees, and Japanese apricots. The three plants are called Sehansamwoo(歲寒三友) showing constant fidelity and loyalty in any changing situations, which was regarded to represent the nature of scholars. And also the study examined the frequency and planting location of the plants in Soswaewon by comparing 48 Yeong and Soswaewondo, and the result showed some differences between them. That seems to be caused by the limitations in the comparison, because in case of 48 Yeong, the location and frequency of landscape plants can be examined only through the context of the verses, and Soswaewondo showed different production time from 48 Yeong and expressed them only in a form of drawing. The plants have symbolic meanings multi-layered and ambiguous. With their symbolic meanings, the landscape plants reviewed through 48 Yeong consistently represent fidelity and loyalty, man of virtue, and hermit. That is, Soswaewon is the garden granting some significance to its compositions by interacting with the things.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.511-525
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• 2015

The purpose of this study is to offer the flora data for conserving the biodiversity of Gyeongju National Park by objective surveying and analysing the flora distributed in Mt. Gumi District, Gyeongju National Park, South Korea. The flora of Mt. Gumi were surmmarized as 476 taxa including 97 families, 297 genera, 419 species, 3 subspecies, 46 varieties and 8 forms. The rare plants were 6 taxa such as Aristolochia contorta, Paeonia lactiflora var. trichocarpa, Potentilla discolor, Berchemia berchemiaefolia, Trigonotis icumae and Iris odaesanensis. The Korean endemic plants were 9 taxa such as Carpinus laxiflora, Pseudostellaria coreana, Philadelphus schrenkii, Indigofera koreana, Lespedeza maritima, Vicia chosenensis, Galium koreanum, Lonicera subsessilis and Weigela subsessilis. The specific plants by floristic region were 38 taxa such as Salix chaenomeloides(I), Hylomecon vernalis (I), Oxalis obtriangulata (I), Viola orientalis(II), Adoxa moschatellina (II), Cirsium chanroenicum (II), Vitex negundo var. incisa (III) and so forth. The naturalized plants were 36 taxa such as Fallopia dumetorum, Chenopodium album, Lepidium virginicum, Amorpha fruticosa, Euphorbia supina, Carduus crispus, Erigeron strigosus, Festuca myuros and so forth. The invasive aline plant was Aster pilosus. The target plants adaptable to climate change were 8 taxa such as Artemisia rubripes, Carpesium macrocephalum and so forth.

• Korean Journal of Plant Resources
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• v.20 no.1
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• pp.28-37
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• 2007

The flora of vascular plants of these sites were listed 272 taxa; 80 families, 193 genera, 234 species, 32 varieties, 5 forms and 1 hybrid. The naturalized plants were 12 taxa, and naturalization rate was 4.4%. Specific plants by floral region were total 54 taxa; 4 taxa in class IV, 10 taxa in class III, 5 taxa in class II, 35 taxa in class I. There is no taxa in class V. A peculiar point is that we found more than 30 Allium pseudojaponicum in Allium genus which is known as Japanese endemic plant. In Gokdudo, there is large Carex boottiana community, in Daehangdo, Machilus thunbergii community covers the whole island. In Bulgeundo, most of the hundreds of trees including Neolitsea sericea and Machilus thunbergii planted by Korea National Parks in 2003 have been damaged by rabbits.

• Applied Biological Chemistry
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• v.37 no.4
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• pp.248-254
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• 1994

Bromoxynil is an antidicot herbicide widely used on cereal crops and has a short half life in the soil. A bxn gene, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was inserted in plant binary vector pGA482, and then introduced into tobacco and lettuce plants via Agrobacterium mediated leaf-disc transformation method. Transgenic plants with the bxn gene were selected by kanamycin and regenerated to whole plants. The regenerated transgenic plants were determined level of expression of bxn gene by Northern blot analysis. Leaf-disc analysis and pot-assay confirmed that the transgenic tobacco and lettuce plants were resistant to high doses of bromoxynil.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.4
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• pp.47-71
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• 2009

The resource plants of northern region in Gapyeong-gun were investigated 7 times from Apr. 2007 to Oct. 2007. In the result of survey, the flora of this area, depend on voucher specimens, consist of 480 taxa totally; 94 families, 286 genera, 412 species, 4 subspecies, 52 varieties and 12 forms. The Resource plants in this area were categorized by their use into edible 177, pasturing 136, medicinal 124, stainable 69, ornamental 133, afforest 24, timber 10, fiber 6, industrial 2 taxa and unknown use plants 72, respectively. There were remarkable plants such as 14 taxa of Korean endemic plants, 85 taxa of specific plants which were designated by the Ministry of Environment, and 12 taxa of rare and endangered plants of Korea Forest Service. Furthermore, 22 taxa of naturalized plants 4.6% were also observed in this area and Urban Index (UI) was 8.2%. The vegetation status of investigated area was comparatively well conserved, but the degree of UI could be increased rapidly by the climbers, development of forest road. Therefore, the systems for conservation need to prepared for the effective management of forest, and the special plans need to control the number of climbers and visitors.