• Molecules and Cells
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• 제42권5호
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• pp.386-396
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• 2019

Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing the subtle behavior of the protein and assessing its cellular function. Over the last decade, many strategies have been developed to achieve selective labeling of proteins in living cells. In particular, the site-specific unnatural amino acid (UAA) incorporation technique has gained increasing attention since it enables attachment of various organic probes to a specific position of a protein in a more precise way. In this review, we describe how the UAA incorporation technique has expanded our ability to achieve site-specific labeling and visualization of target proteins for functional analyses in live cells.

• BMB Reports
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• 제28권5호
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• pp.433-436
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• 1995

In order to assign NMR signals, conditions for the specific labeling of cytochrome $c_3$ of D. vulgaris Miyazaki F through the culture in a minimal medium were established. Phenylalanine residue was specifically deuterated at more than 85% efficiency. Cytochrome $c_3$ has two phenylalanine residues. The signals of one phenylalane were missing and this was tentatively assigned to Phe20.

• Archives of Pharmacal Research
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• 제27권9호
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• pp.961-967
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• 2004

The development of an antibody labeling method with $^{99m}$Tc is important for cancer imaging. Most bifunctional chelate methods for $^{99m}$Tc labeling of antibody incorporate a $^{99m}$Tc chelator through a linkage to lysine residue. In the present study, a novel site-specific $^{99m}$Tc labeling method at carbohydrate side chain in the Fc region of 2 antibodies (T101 and rabbit anti-human serum albumin antibody (RPAb)) using dihydrazinophthalazine (DHZ) which has 2 hydrazino groups was developed. The antibodies were oxidized with sodium periodate to pro-duce aldehyde on the Fc region. Then, one hydrazine group of DHZ was conjugated with an aldehyde group of antibody through the formation of a hydrazone. The other hydrazine group was used for labeling with $^{99m}$Tc. The number of conjugated DHZ was 1.7 per antibody. $^{99m}$Tc labeling efficiency was 46-85％ for T101 and 67∼87％ for RPAb. Indirect labeling with DHZ conjugated antibodies showed higher stability than direct labeling with reduced antibodies. High immunoreactivities were conserved for both indirectly and directly labeled antibodies. A biodistribution study found high blood activity related to directly labeled T1 01 at early time point as well as low liver activity due to indirectly labeled T101 at later time point. However, these findings do not affect practical use. No significantly different biodistribution was observed in the other organs. The research concluded that DHZ can be used as a site-specific bifunctional chelating agent for labeling antibody with $^{99m}$Tc. Moreover, $^{99m}$Tc labeled antibody via DHZ was found to have excellent chemical and biological properties for nuclear medicine imaging.edicine imaging.

• 농업과학연구
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• 제46권2호
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• pp.323-333
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• 2019

In Korea, origin labeling is one of the main issues in the food service sector. Many restaurants presented the incorrect or no origin of the food material, and Korean consumers have kept complaining about it. Even though the origin labeling program was welcomed by consumers, the food service sector has claimed that there is an increasing cost due to the origin labeling program. It is an important issue to determine whether the origin labeling program is good for the social welfare; however, the specific effects of the origin labeling program have rarely been measured. The purpose of this study was to measure the effect of origin labeling of beef in the Korean food service sector. Through survey and model analyses, a few findings are presented. First, Korean consumers showed a positive willingness-to-pay for the origin labeling of beef. Especially, consumers without information on the origin of the beef showed a larger willingness-to-pay for the origin labeling. Second, the origin labeling of beef changed the price and quantity of beef, and this change became larger when the consumers had no information on the origin of the beef. The change in the marketing margin due to the origin labeling program also affected the changes in the price and quantity of the beef. Third, the origin labeling of beef increased the social welfare, which is the sum of the consumer surplus and producer surplus. And this increase of the social welfare became larger when the consumers had no information about the origin.

• 대한인간공학회지
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• 제15권1호
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• pp.105-117
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• 1996

This study was initiated to investigate (1) whether consumers preferred a specific garment size labeling system and (2) whether consumer's demographic characteristics, clothing behavior, and body size affect their preference of size labeling systems. The survey of consumer's preferences for five size labeling systems included 443 women with 20 to 64 years of age. The result of the survey showed that the subjects' preferences of five size labeling sysems were different significantly. Subjects strongly preferred the old system rather than the revised systems. The subjects least preferred the system with pictogram(system D) and the system with description of the measuring methods of te control dimensions (system E). The subjects/ preference for various garment size labeling systems was different depending on their resident area, educational level, and body size. The garment size labeling system with more information on body measurements (system C) was preferred by the subjects living in the metro city area or subjects having difficulties on purchasing ready-to-wear garments giving reasonable fit. The simplest size labeling system (system A) was strongly preferred by the subjects of low educational level, or the subjects living in the small city.

• BMB Reports
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• 제37권2호
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• pp.260-267
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• 2004

Transducin (T), the heterotrimeric guanine nucleotide binding protein in rod outer segments, serves as an intermediary between the receptor protein, rhodopsin, and the effector protein, cGMP phosphodiesterase. Labeling of T with dansyl chloride (DnsCl) inhibited its light-dependent guanine nucleotide binding activity. Conversely, DnsCl had no effect on the functionality of rhodopsin. Approximately 2-3 mol of DnsCl were incorporated per mole of T. Since fluoroaluminate was capable of activating DnsCl-modified T, this lysine-specific labeling compound did not affect the guanine nucleotide-binding pocket of T. However, the labeling of T with DnsCl hindered its binding to photoexcited rhodopsin, as shown by sedimentation experiments. Additionally, rhodopsin completely protected against the DnsCl inactivation of T. These results demonstrated the existence of functional lysines on T that are located in the proximity of the interaction site with the photoreceptor protein.

• 무역학회지
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• 제44권2호
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• pp.143-157
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• 2019

The purpose of this study is to review domestic and foreign origin labeling system in order to implement origin labeling system in the perspective of protecting the interests of consumers, and to suggest governmental improvements by analyzing problems embedded in current labeling system. The results analysis show complexity of related legal system, lack of expertise at the stage of labeling, and inefficiency of crackdown authority. The improvement could be suggested in two ways: supporting plans for the ones who have duty of labeling and improvement plans in origin management system. As supporting plans, we suggest the need for an automatic origin determination system, appropriate education on origin stakeholders, and introduction of origin certification system. For improvement plans, there are unification of country of origin labeling related laws, utilization of FTA product specific rules, and QR code, expert confirmation system. Since the origin labeling issue has become important, proactive and quick responses must follow with thorough examination the effect of the origin labeling on consumer welfare.

• 한국식생활문화학회지
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• 제10권3호
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• pp.155-166
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• 1995

Consumer awareness of current food labeling system and new nutrition labeling system which the government considers to adopt widely was assessed, from May to June in 1994, for 423 adults living in Seoul. Being highly conscious of food safety, they thought the current food label did not provide sufficient information. Dissatisfaction with the current system was shown higher in the female, young, or unmarried strata. The need for nutrition labeling system was widely recognized by 82% of total respondents regardless of sex, level of education, monthly income, and marital status. Although some expressed worries about proper management of the system, most of the respondents answered that the system would benefit them after all. In this connection, 59% of the respondents showed willingness to accept a price increase to be entailed by nutrition labeling. Nutrition informations that, they thought, should be covered were: calorie>minerals>cholesterol>protein>vitamins>fat>sodium. Additional labeling informations they called for were nutrient contents>RDA percentage>specific statement on reinforced or eliminated nutrients.

• 한국동물학회지
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• 제33권2호
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• pp.141-151
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• 1990

Raf protein kinases and protein kinase C는 세포질내 serine/threonine-specific protein에 속한다. 그리고 기능적인 구조와 세포내의 분포 양상은 서로 비슷하다. Raf family oncogene를 발현시키는 a-raf와 c-raf protein kinase에 대한 antibodies로써 남생이 대뇌의 raf protein kinase의 분포를 조사하였다. 일반적으로 raf protein kinase는 제한된 지역에서 즉,general pallium,hippocampal formation, pdmordiuin hippocampi,nucleus of lateral olfactory tract, basal amygdaloid nucleus와bed of stria terminalis에 나타났으며, c-raf protein kinase의 면역학적 labeling은 a-raf보다 그 범위가 넓었다. 그렇지만 labeling되는 intensity는 오히려 a-raf보다 낮았다. 그런데 a-raf에서 가장 명확한 좋은 예는 basal amygdaloid nucleus내의 구형모양의 세포인데, 이 세포는 세포질이 매우 강하게 labeling되어 지므로 ring모양과 같이 나타났다. 특히 c-raf는 protein kinase C 가 많이 나타나는 pyramidal 세포나 Purkinje세포에 많이 존재하는 것을 볼 때 protein kinase에 의하여 활성화되는 myc와 서로 상협작용을 유도한다고 제안하는 바이다.

• Fisheries and Aquatic Sciences
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• 제26권11호
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• pp.678-688
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• 2023

Flatfish are one of the largest families in the order Pleuronectiformes and are economically important edible marine fish species. However, they have similar morphological characteristics leading to challenges in classifying correctly, which may result in mislabeling and illegal sales, such as fraudulent labeling of processed food. Therefore, accurate identification is important to ensure the quality and safety of domestic markets in Korea. Species-specific primers were prepared from the mainly consumed eleven species of the order Pleuronectiformes. To rapidly identify the 11 flatfish species, a highly efficient, rapid, multiplex polymerase chain reaction (PCR) with species-specific primers was developed. Species-specific primer sets were designed for the mitochondrial DNA cytochrome c oxidase subunit I gene. Species-specific multiplex PCR (MSS-PCR) either specifically amplified a PCR product of a unique size or failed. This MSS-PCR analysis is easy to perform and yields reliable results in less time than the previous Sanger sequencing methods. This technique could be a powerful tool for the identification of the 11 species b the family Pleuronectidae and can contribute to the prevention of falsified labeling and protection of consumer rights.