• Animal cells and systems
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• 제4권1호
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• pp.1-7
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• 2000

The genus Dendronephthya, generally known as soft corals, is reported as an abundant and variable taxon. They mostly distribute in warmer waters of the Undo-Pacific Ocean region including Korea. In spite of their abundance and ecological importance as habitats of marine organisms, there are difficulties in the study of their identification and systematics because they have morphological variabilities and limited taxonomec characters. To resolve the problems, we attempted to elucidate the genetic relationships in the genus Dendronephthya by using random amplified polymorphic DNA (RAPD) analysis. This study was based on eight dendronephthian species and one Alcyoniidae species, Alcyonium gracillimum, as an outgroup. The results from all analysis suggest that they could be classified into four groups by the growth form and the anthocodial grades as follows: the first one,D. putteri and D. suensoni with the divaricate form and VI grade; the second one,D. sp.1 and D. sp.2 with the divaricate form and III or IV grade; the third one, D. gigantea and D. aurea being closer than D. spinifera with the glomerate form and III grade; the last one, D. castanea related to D. gigantea rather than D. putteri with the umbellate form and IV grade. Moreover, the divaricate form was separated from the group of the glomerate and umbellate form. At the intraspecies level, the types of the D. castanea, D. gigantea and D. spinifera were separated depending on the feature of spicules in the polyp head, and the coloration could not influence genetic variation. From this study, we can confirm that their morphological characters are compatible with the genetic variation, also RAPD analysis is a very useful method for resolving the systematic relationships of den-deonephthians.

• The Korean Journal of Ecology
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• 제21권1호
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• pp.105-112
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• 1998

Characteristics of species distribution in the Baekdoo Great Mountain Chain of Kangwon Province were discussed. Resulting from Detrending Correspondence Analysis based on the floristic composition of 48 mountains, the mountains of the Baekdoo chain tended to aggregate on DCA axis. it represented that there was the peculiar floristic composition of the Baekdoo chain separated from the other mountains. High altitude, longitude near the East Sea and large number of vascular species were main factors for the distribution pattern. And exponential relationships between endemic rare species and number of species were clarified. Even though the regions of the Baekdoo Great Mountain Chain had been conserved relatively well, many regions have been faced to be disturbed for tourism, agriculture, forestry, livestock industry and mining since the local autonomy. Thusm, the necessity for the establishment of large area network through the connection of scattered protected areas in the Baekdoo Great Mountain Chain was suggested. it would play a role as a core of nature conservation to sustain species diversity in Korea.

• 수산경영론집
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• 제44권1호
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• pp.71-79
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• 2013

This study is aimed to estimate market integration of wild caught fish species on the Korean market, using both multivariate and bivariate cointegration analysis. For the analysis of market integration between wild caught fish species, major four fish species those are most popular fish in the market and caught by the large purse seine fishery-chub mackerel, jack mackerel, hairtail and spanish mackerel-were selected as analytical target fish species. And their real monthly price data from January 2000 to December 2011 were used in the analysis. The results of the multivariate cointegration test for four wild caught fish species showed that there would be long-term equilibrium relationships among prices of four wild caught fish species, and consequently, the markets for wild caught fish species were estimated to be integrated. The results of exclusion test and bivariate cointegration test also supported that there would be a clear evidence to suggest that all target wild caught fish species were cointegrated each other.

• Journal of Microbiology
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• 제38권2호
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• pp.62-65
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• 2000

Partial sequence comparisons of mitochondrial small subunit rDNA (mt SSU rDNA) were used to examine taxonomic and evolutionary relationships among seven Penicillium species : two monoverticillate species, two biverticillate species, and three terverticillate species. Amplified fragments of mt SSU rDNA highly varied among seven species in size, suggesting the existence of multiple insertions or deletions in the region. A phylogengtic tree was constructed by exhaustive search of parsimony analysis. The phylogenetic tree distinguished two statistically supported monophyletic groups, one for two monoverticillate species and the other for three terverticillate species and ont biverticillate species, P. vulpinum. The phylogenetic relationship of P. waksmanii, the biverticillate species, was not clear.

• 한국양식학회지
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• 제21권4호
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• pp.346-350
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• 2008

Haliotis속 전복류 내에서 종간의 유전적 차이 및 유연관계를 나타내는 유전표식으로 RAPD를 사용하여 그 유용성 정도를 파악하기 위하여 북반구 2종(H. discus hannai와 H. rufescens)과 남반구 2종(H. rubra와 H. midae)을 대상으로 RAPD 분석을 행하였다. 그 결과 RAPD는 Haliotis 종간의 구분은 명확히 구분할 수 있는 유용한 유전표식으로 평가되었으나, 유전적 유연관계에 있어서는 형태학적 및 지리학적 분포에 따른 유연관계와는 상이하게 나타났다.

• 한국약용작물학회지
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• 제12권3호
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• pp.214-218
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• 2004

우리 나라 전역에서 수집한 7종의 자생 둥굴레로부터 genomic DNA을 추출한 후 Operon random primer(10-mer)를 이용하여 PCR을 수행하여 재현성이 있으면서 polymorphism을 보이는 19개의 primer를 선발하였다. 선발한 primer로부터 PCR에 의해 증폭된 DNA의 크기는 $3000{\sim}300\;bp$이었고, 19개의 random primer에서 복제된 전체 band의 수는 114개에서 157개로 공시종간에 많은 차이를 보였으며, band의 유무에 근거한 유연관계 분석에서 7개의 공시종은 각시둥굴레와 층층둥굴레가 1개군으로, 여타 종들을 1개군으로 크게 2개군으로 분리되어 유전적으로 상당한 차이가 있는 것으로 나타났다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 공동 춘계학술대회
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• pp.45-45
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• 1997

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 공동 춘계학술대회
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• pp.65.2-66
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• 1997

No Abstract, See Full Text

• Animal Systematics, Evolution and Diversity
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• 제11권2호
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• pp.207-221
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• 1995

남한해역에서 채집된 Raja속 어류를 Ishihara(1987)의 분류기준에 따라 동정한 결과 Raja(Okamejei) meerdervoortii Bleeker(오동가오리), R.(O.) acutispina Ishiyama(국명신칭:무늬홍어), R.(O.) kenojei Muller et Henle(홍어), R.(Dipturus) kwangtungensis Chu(국명신칭: 참홍어), R.(D.)pulchra Liu(눈가오리)등 5종이 확인되었다. 5종 10집단 165개체에 대한 전기영동법을 이용한 동위효소 분석 결과 13개의 효소 및 비효소성 단백질에서 24개의 유전자를 검출하고 대립인자 빈도를 산출하여 종간 비교를 실기하였다. 5종 중 R.(O.) kenojei 집단은 4집단 간 평균 S = 0.966으로 매우 가까운 유전적 근연치를 나타냈으며, R.(O.) meerdervoortii와 R.(O.) acutispina는 유전적 근연치가 S = 0.749였으며, R.(O.) kenojei와 R.(D.) kwangtungensis는 S = 0.829였으나, R.(D.) pulchra는 다른 4종과 S = 0.597의 값을 나타내어 유전적 근연관계가 제일 멀게 나타났다. R.(D.) kwangtungensis의 경우 동일 아속인 Dipturus 아속에 속하는 R.(D.) pulchra와는 유전적 근연치가 S = 0.648로 뚜렷한 차이가 있었으나, Okamejei 아속에 속하는 R.(O.) kenojei와는 S = 0.829의 가까운 유전적 근연치를 나타내어 주목된다. R.(O.) acutispina와 R.(D.) kwangtungensis는 한국 미기록종으로 동정되었다.

• Molecules and Cells
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• 제25권4호
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• pp.510-522
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• 2008

To construct the molecular systematics of the genus Megoura (Hemiptera: Aphididae), DNA based-identification was performed using four mitochondrial and three nuclear DNA regions: partial cytochrome c oxidase I (COI), partial tRNA-leucine + cytochrome c oxidase II (tRNA/COII), cytochrome b (CytB), partial 12S rRNA + tRNA-valine + 16S rRNA (12S/16S), elongation factor-1 alpha ($EF1{\alpha}$), and the internal transcribed spacers 1 and 2 (ITS1, ITS2). Pairwise sequence divergences between taxa were compared, and phylogenetic analyses were performed based on each DNA region separately, and the combined datasets. COI, CytB, $EF1{\alpha}$, ITS1, and ITS2 were relatively effective in determining species and resolving their relationships. By contrast, the sequences of tRNA/COII and 12S/16S were not able to separate the closely related species. CytB and $EF1{\alpha}$ gave better resolution with higher average sequence divergences (4.7% for CytB, 5.2% for $EF1{\alpha}$). The sequence divergence of COI (3.0%) was moderate, and those of the two ITS regions (1.8% for ITS1, 2.0% for ITS2) were very low. Phylogenetic trees were constructed by minimum evolution, maximum parsimony, maximum likelihood, and Bayesian phylogenetic analyses. The results indicated that the phylogenetic relationships between Megoura species were associated with their host preferences. Megoura brevipilosa and M. lespedezae living on Lespedeza were closely related, and M. nigra, monophagous on Vicia venosa, was rather different from M. crassicauda, M. litoralis, and M. viciae, which are oligophagous on Lathyrus and Vicia. The three populations of M. crassicauda formed a clade separated from M. litoralis and M. viciae. Nevertheless M. litoralis and M. viciae, which are morphologically similar, were not separated due to negligible sequence divergence. We discuss the phylogenetic relationships of the Megoura, and the usefulness of the seven DNA regions for determining the species level phylogeny of aphids.