• Ecology and Resilient Infrastructure
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• v.6 no.1
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• pp.58-68
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• 2019

This present study conducted the impact of the restoration of low-flow channel on fish habitat in a reach of the Wonju Stream, Korea. The target species were Zacco koreanus, a dominant species in the middle- and upper-stream of Wonju Stream. The River2D model was used for the computation of the flow and the habitat suitability index model was used to estimate the quantity and quality of fish habitat using habitat suitability curves. In order to restore the study area, the low-flow channel was identified through the bankfull discharge of the study reach, and the best hydraulic section was designed. In addition, various restoration techniques were applied to the low-flow channel through rocks, pool/riffle structures, and the change of the channel width. Simulation results indicated that the restoration of the best hydraulic section effects decreased habitat suitability for the target species. However, when various restoration techniques were applied, the WUA (Weighted Usable Area) was improved by about 11%.

• International Journal of Oral Biology
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• v.35 no.3
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• pp.129-135
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• 2010

Recent studies have implicated reactive oxygen species (ROS) as determinants of the pathological pain caused by the activation of peripheral neurons. It has not been elucidated, however, how ROS activate the primary sensory neurons in the pain pathway. In this study, calcium imaging was performed to investigate the effects of NaOCl, a ROS donor, on the intracellular calcium concentration ($[Ca^{2+}]i$) in acutely dissociated dorsal root ganglion (DRG) neurons. DRG was sequentially treated with 0.2 mg/ml of both protease and thermolysin, and single neurons were then obtained by mechanical dissociation. The administration of NaOCl then caused a reversible increase in the $[Ca^{2+}]i$, which was inhibited by pretreatment with phenyl-N-tertbuthylnitrone (PBN) and isoascorbate, both ROS scavengers. The NaOCl-induced $[Ca^{2+}]i$ increase was suppressed both in a calcium free solution and after depletion of the intracellular $Ca^{2+}$ pool by thapsigargin. Additionally, this increase was predominantly blocked by pretreatment with the transient receptor potential (TRP) antagonists, ruthenium red ($50\;{\mu}M$) and capsazepine ($10\;{\mu}M$). Collectively, these results suggest that an increase in the intracellular calcium concentration is produced from both extracellular fluid and the intracellular calcium store, and that TRP might be involved in the sensation of pain induced by ROS.

• Korean Journal of Ichthyology
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• v.28 no.1
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• pp.47-51
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• 2016

Three specimens of Abudefduf septemfasciatus were collected firstly from tidal pools on the southern and northern coasts of Jeju Island, Korea in August 2015. This species is characterized by black blotches on the upper origin of the pectoral fin, upper caudal peduncle and a dorsal fin between the second and fourth spines, predorsal scales beyond to above the middle of the eye, 12~13 soft dorsal and anal fin rays, and 20 lateral-line scales. This species is distinguishable from Abudefduf sordidus by soft dorsal and anal fin rays, location of caudal peduncle blotch, and location of the dorsal fin blotch. The newly proposed Korean name is "Il-gop-jul-ja-dom".

• Animal cells and systems
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• v.16 no.1
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• pp.69-76
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• 2012

This study was conducted to examine how a low-head dam removal (partial removal) could affect benthic macroinvertebrate communities in a stream. Benthic macroinvertebrates and substrates were seasonally sampled before and after dam removal (March 2006-April 2007). Benthic macroinvertebrates and substrates were quantitatively sampled from immediately upstream (upper: pool) and downstream (lower: riffle) sites, the location of the dam itself (middle), and immediately above the impoundment (control: riffle). After the removal, species richness and density of benthic macroinvertebrates as well as the EPT group (Ephemeroptera, Plecoptera and Trichoptera) increased to higher levels than those before the removal, while functional feeding groups and habitat orientation groups changed more heterogeneously at the upper site. At the lower site, species richness and density decreased somewhat immediately after dam removal, which was associated with an increase of silt and sand, but recovered after monsoon floods which helped to enhance substrate diversity at the upper site. Decreased dominance index and increased diversity index in both the upper and lower sites are evidence of positive effects from the dam removal. In conclusion, we suggest that even a partial removal of a dam, resulting in increased substrate diversity in the upper site, could sufficiently help rehabilitate lost ecological integrity of streams without major habitat changes.

• The Korean Journal of Ecology
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• v.19 no.3
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• pp.217-222
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• 1996

For the ecological study on the gobioid (Favonigobius gymnauchen), samples were collected in the Korean coasts from 1983 to 1995, and the process of ovarian maturation, spawning season, settling period of young individuals and growth were investigated with the specimens collected from Kunsan coast. The ovarian egg development of this species underwent three stages; growth stage from March to April, maturity stage from May to June and spawning stage in July. All the adults died after spawning in late July. Young individuals of total length 10 mm began to live a bottom life in the tide pool of shallow waters in early and middle August. The total length of these individuals reached about 42.1 mm (mean 36.7 mm) in late November. The largest specimen examined in this study was 85.0mm of male. After that time, individuals of this species inhabited in subtidal zone from December to May of the next year. The Favonigobius gymnauchen is distributed at 17 areas of shallow waters and estuaries in the western and southern coasts of Korea.

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.367-379
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• 2013

The distribution of Coreoperca kawamebari was known to be restricted to the Tamjin River and several small streams flowing into the southern part of the West Sea, while C. herzi was widely distributed throughout South Korea except the areas inhabited by C. kawamebari: the two species were known to be allopatric. However, we found that both species were sympatric in the Boseong River, a tributary of the Seomjin River, and the Jiseok Stream, a tributary of the Yeongsan River. Local-scale distribution of the two Coreoperca species, fish assemblages, and environmental variables were surveyed to investigate effects of environmental factors on fish community structures in the Boseong River. Rank abundance distribution of fish community at the study sites indicated that fish species diversity and distribution pattern of the two Coreoperca species were closely related to habitat diversity. The result of canonical correspondence analysis showed that C. kawamebari was distributed in pool areas while C. herzi was found in the areas with higher water velocity and boulder substrate. These results suggested that species diversity of fish community decreased and only one of the two Coreoperca species inhabited at the sites with less diverse habitat, but on the other hand, high habitat diversity increased species diversity and allowed the two species to coexist.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.43-49
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• 1999

To develope wild flower collected from Chollabuk-do area resource plant species were investigated from May 1996 to April 1997. This study was focussed to make the basic data for better quality of breeding species from wild flower species and to keep proper gene pool for development with competitive power internationally in future. There was 131 species blooming in spring season(34.7%), 219 species in summer(58.1%), 25 species in autumn(6.6%) and 2 species in winter(0.5%), respectively. Flower color of all the plants was 26 kinds in diverse, among them, white flowers were occupied dominantly of 28.9%, yellow flowers, 20.7%, violet flowers, 16.4% and red purple flowers, 7.9%, respectively. Transplanted wild flower plants at the garden in the study area frequently were Chelidonium majus var. asiaticum, Hemerocallis fulva, Iris nertschinskia, Rhododendron yedoense var. poukhanense, Rhododendron mucronulatum, Pulsatilla koreana, Chrysanthemum zawadskii var. latilobum, Taraxacum platycarpum, Chrysanthemum boreale, Liriope platyphylla and Lycoris radiata. Twenty-six species of wild flowers such as Cymbidium goeringii, H. fulva, L. radiata, C. zawadskii var. latilobum, etc. were collected from their habitat and being selling at the market or road side. Total 343 taxa of wild flowers to prevent carrying out to foreign countries and to improve better quality were selected and recommended in this study, for example, they were 41 taxa of Compositae plant, 25 of Rosaceae, 20 of Liliaceae and 18 of Ranunculaceae in order. Acorus calamus var. angustatus, Cypripedium japonicum, C. macranthum, Calanthe discolor, Dicentra spectabilis, Ilex cornuta, Stewartia koreana, Abeliophyllum distichum, Forsythia saxatilis and Campanula glomerata var. dahurica should be protected as wild flowers because their numbers are decreasing remarkably, belong to rare species, being at a crisis to extinct species and important plants for research materials.

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.81-88
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• 2014

Salt cress (Thellungiella halophila or Thellungiella parvula), species closely related to Arabidopsis thaliana, represents an extremophile adapted to harsh saline environments. To isolate salt-tolerance genes from this species, we constructed a cDNA library from roots and leaves of salt cress plants treated with 200 mM NaCl. This cDNA library was subsequently shuttled into the destination binary vector [driven by the cauliflower mosaic virus (CaMV) 35S promoter] designed for plant transformation and expression via recombination- assisted cloning. In total, 305,400 pools of transgenic BASTA-resistant lines were generated in Arabidopsis using either T. halophila or T. parvula cDNA libraries. These were used for functional screening of genes involved in salt tolerance. Among these pools, 168,500 pools were used for primary screening to date from which 7,157 lines showed apparent salt tolerant-phenotypes in the initial screen. A secondary screen has now identified 165 salt tolerant transgenic lines using 1,551 (10.6%) lines that emerged in the first screen. The prevalent phenotype in these lines includes accelerated seed germination often accompanied by faster root growth compared to WT Arabidopsis under salt stress condition. In addition, other lines showed non-typical development of stems and flowers compared to WT Arabidopsis. Based on the close relationship of the tolerant species to the target species we suggest this approach as an appropriate method for the large-scale identification of salt tolerance genes from salt cress.

• Biomedical Science Letters
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• v.18 no.3
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• pp.324-327
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• 2012

Acanthamoeba is a free-living amoeba that causes human infections, and recently the incidence of amoebic keratitis has increased among contact lens wearers. In order to investigate Acanthamoeba contamination of contact lens storage cases, a short survey was performed on 57 contact lens wearers, and Acanthamoeba was found in one contact lens storage case. To diagnose Acanthamoeba, the 18s small subunit ribosomal DNA (18s rDNA) gene was amplified by polymerase chain reaction (PCR), and subsequently, the isolate was identified as A. lugdunensis. This species was originally isolated from a freshwater pool in France, and was reported recently to be a cause of amoebic keratitis. This observation indicates the need for a large survey to investigate the extent of Acanthamoeba contamination, and suggests that contact lens wearers be aware of the importance of hygiene and of the implications of Acanthamoeba infection.

• Journal of Industrial and Engineering Chemistry
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• v.67
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• pp.231-235
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• 2018

In this study, we demonstrate the conversion of starch to (R,R)-2,3-butanediol (2,3-BD) in a hybrid cell-free synthesis system containing a mixture of lysates derived from Escherichia coli (E. coli) and cyanobacteria. A sufficient pool of pyruvate required for the synthesis of 2,3-BD was generated by combining metabolic pathways of cyanobacteria and E. coli. Successful synthesis of 2,3-BD was achieved by additional modifications of the hybrid cell-free system with the enzymes required to convert pyruvate to 2,3-BD. The results demonstrate a new approach to harness biological pathways to expand the scope of cell-free metabolic engineering by cross-species combinations of cell lysates.