• Journal of Periodontal and Implant Science
• /
• 제34권4호
• /
• pp.747-757
• /
• 2004

The effect of chitosan, a carbohydrate biopolymer extracted from chitin, on periodontal regeneration is of particular interest. The purpose of this study was to evaluate the effect of chitosan on primary rat calvarial cells in vitro, with special focus on their proliferative properties by cell activity and the amount of total protein synthesis. The experimental groups were cultured with chitosan in concentration of 0.01, 0.1, 1.0, 2.0 and 5.0 mg/ml for MTT assay. In the experimental groups, cells were cultured with chitosan in concentration of 0.01, 0.1, 1.0 and 2.0 mg/ml. Each group was characterized by examining alkaline phosphatase activity at 3 and 7 days and the ability to produce mineralized nodules of rat calvarial cells at 14 and 21 days. The results were as follows: 1. The cell activity was not reduced in the concentration of $0.01{\sim}1.0$ mg/ml whereas the cell activity was reduced in the concentration of 5.0 mg/ml than the control at day 1 and 3 (p<0.05). 2. Primary rat calvarial cells treated with chitosan in the concentration 0.01 mg/ml and 0.1 mg/ml showed more protein synthesis than the control at day 3 (p<0.01), But primary rat calvarial cells treated with chitosan showed more protein synthesis than in control but they didn't have statistically difference among groups at day 7. 3. At 3 and 7 days, alkaline phosphatase activity was significantly increased in the concentration of 0.01 mg/ml. 0.1 mg/ml and 1.0 mg/ml (p<0.05). 4. The percentage of mineralized bone nodule was more in the concentration of chitosan 0.1 mg/ml and 1.0 mg/ml than the control. These results suggested that chitosan has a positive effect on the bone formation of primary rat calvarial cells in the concentration of 0.1 mg/ml and 1.0 mg/ml.

• 한국자원식물학회지
• /
• 제28권2호
• /
• pp.243-252
• /
• 2015

본 연구는 제주특산식물인 좀향유의 현지 내･외 보존을 위한 기초 연구로서 증식체계를 마련하고 전처리제 처리에 따른 발근 특성과 시비에 따른 유묘의 생장 효과를 등을 알아보았다. 온실과 밀폐상의 온습도 조사결과 평균 온도는 비슷하였으나 공중 습도는 온실보다 밀폐상이 높은 습도를 유지하였으며, 좀향유 삽목 후 온실은 10일, 밀폐상은 7일만에 최초 발근을 확인하여 밀폐상의 빠른 발근율을 보였으며 온실과 밀폐상에서의 생존율, 발근율, 뿌리수 등은 근소하게 밀폐상이 높게 조사되어 온실보다 밀폐상이 효과적이었다. 발근억제물질 제거를 위한 전처리제 처리는 AgNO₃ 77.5%를 제외한 다른 처리구에서 95% 이상의 높은 발근율로 조사되어 좀향유의 경우 발근억제 물질은 적은 것으로 판단된다. 유묘의 용기별 질소 시비량 및 Osmocote 시비시험 결과 시비량과 용기의 조건에 따라 생존율과 생장 등에 차이를 보였다. 배양판에서 완효성 비료인 Osmocote를 시비한 결과 생존율, 지상부, 지하부 생장 등이 가장 양호하여 좀향유의 유묘 증식을 위해서는 Osmocote 시비가 바람직 할 것으로 판단된다.

• 응용통계연구
• /
• 제32권1호
• /
• pp.161-171
• /
• 2019

일별 최대전력 수요 예측은 국가의 전력 수급운영에 중요한 과제로서 과거부터 다양한 방법들이 끊임없이 연구되어 왔다. 일별 최대전력 수요를 정확히 예측함으로써 발전설비에 대한 일일 운용계획을 작성하고 효율적인 설비 운용을 통해 불필요한 에너지 자원의 소비를 감소하는데 기여할 수 있으며 여름 겨울철 냉난방수요로 인해 발생하는 전력소비 과다로 인한 전력예비율 감소 문제 등에 선제적으로 대비할 수 있는 장점을 가진다. 이러한 일별 최대전력수요 예측을 위하여 본 논문에서는 Seasonal ARIMA, TBATS, Seasonal Reg-ARIMA, NNETAR 모형에 평일, 주말, 특수일에 대한 효과와 온도에 대한 영향을 함께 고려하여 다음날의 일별 최대전력을 예측하는 모형을 연구하였다. 본 논문을 통한 모형들의 예측 성능 평가 결과 요일, 온도를 고려할 수 있는 Seasonal Reg-ARIMA 모형과 NNETAR 모형이 이를 고려할 수 없는 다른 시계열 모형보다 우수한 예측 성능을 나타내었고 그 중 인공신경망을 활용한 NNETAR 모형의 예측 성능이 가장 우수하였다.

• 한국방사선학회논문지
• /
• 제18권2호
• /
• pp.119-125
• /
• 2024

본 연구의 목적은 방사능 테러 상황에서 경찰특공대 대테러요원의 방사선 손상을 최소화할 수 있는 방사선 방호제 개발을 위하여 셀레늄과 아르기닌 혼합물의 방사선 방호효과를 규명하는 것이다. 본 연구에서는 72 마리의 수컷 SD rat을 일반군(NC Group), 셀레늄과 아르기닌 혼합물 투여군(SeAr Group), 방사선 노출군(IR Group), 셀레늄과 아르기닌 혼합물 투여 후 방사선 노출군(SeAr+IR Group)의 4개 군으로 분류하였다. 방사선은 7 Gy 엑스선을 전신에 노출하였고, 셀레늄과 아르기닌은 각각 1일 1회 3 mg/kg, 150 mg/kg이 흡수되도록 혼합하여 14일간 경구 투여였다. 방사선 노출 후 1일, 7일, 21일차에 혈액학적 분석과 조직학적 분석을 수행하였다. 혈액학적 분석에서는 IR Group에 비하여 SeAr+IR Group에서 방사선 노출 후 1일차에 림프구(p<0.05)에서, 7일차에 혈소판(p<0.01)에서, 21일차에 적혈구(p<0.01)에서 유의한 방사선 방호가 나타났다. 조직학적 분석에는 IR Group에 비하여 SeAr+IR Group에서 방사선 노출 7일차에 소장에서 소장움 세포의 경계가 적게 무너지고 소장움의 길이가 비교적 회복된 것이 관찰되었고, 21일차에 전립선에서 세포수와 세포벽의 두께가 더 양호한 것으로 관찰되었다. 따라서 셀레늄과 아르기닌 혼합물은 방사선 노출에 따른 혈액 및 조직에 대한 방사선 방호효과가 있는 것으로 판단되며 경찰특공대 대테러요원의 방사선 손상 감소를 위한 방사선 방호제 연구에 유용하게 활용될 것으로 기대된다.

• 한국작물학회지
• /
• 제62권1호
• /
• pp.60-65
• /
• 2017

이 시험은 '솔라라' 품종의 종자생산을 위해 분무경 수경재배에 적합한 묘를 구명하고자 2015년 3월 16일 배양순화묘 등 8처리의 묘를 분무경 베드에 정식하여 정식 후 70, 78일에 각각 생육과 수량특성을 조사하였다. 경장은 경삽 15일묘와 배양순화묘가 작은 편이었고 경삽묘의 육묘일수가 늘어남에 따라 길어지는 경향이었다. 생체중도 경삽 40일묘가 주당 122 g으로 가장 무거웠고 무발근묘와 배양순화묘는 가장 가벼웠다. 1차 복지는 경삽 35일묘에서 주당 12.9개로 가장 많았고 2차 복지는 경삽 35일묘, 배양순화묘, 경삽 30일묘에서 많이 발생하였다. 10주당 총괴경수는 배양순화묘가 145개로 많았으나 3~50 g(상품) 괴경수는 경삽 35일묘가 108개로 가장 많았고, 괴경 무게는 경삽 35일묘가 1,947 g으로 가장 무거웠고 다음으로 경삽 30일묘였다. '솔라라' 품종 수경재배 시에는 경삽한 후 30일~35일(경장 15 cm, 마디 12절) 묘로 정식하는 것이 수량성을 높일 수 있었다.

• Wind and Structures
• /
• 제20권6호
• /
• pp.763-791
• /
• 2015

"Wind and Ports" is a European project that has been carried out since 2009 to handle wind forecast in port areas through an integrated system made up of an extensive in-situ wind monitoring network, the numerical simulation of wind fields, the statistical analysis of wind climate, and algorithms for medium-term (1-3 days) and short term (0.5-2 hours) wind forecasting. The in-situ wind monitoring network, currently made up of 22 ultrasonic anemometers, provides a unique opportunity for detecting high resolution thunderstorm records and studying their dominant characteristics relevant to wind engineering with special concern for wind actions on structures. In such a framework, the wind velocity of thunderstorms is firstly decomposed into the sum of a slowly-varying mean part plus a residual fluctuation dealt with as a non-stationary random process. The fluctuation, in turn, is expressed as the product of its slowly-varying standard deviation by a reduced turbulence component dealt with as a rapidly-varying stationary Gaussian random process with zero mean and unit standard deviation. The extraction of the mean part of the wind velocity is carried out through a moving average filter, and the effect of the moving average period on the statistical properties of the decomposed signals is evaluated. Among other aspects, special attention is given to the thunderstorm duration, the turbulence intensity, the power spectral density and the integral length scale. Some noteworthy wind velocity ratios that play a crucial role in the thunderstorm loading and response of structures are also analyzed.

• 대한한의학회지
• /
• 제28권4호
• /
• pp.114-123
• /
• 2007

Osteoarthritis is characterized by cartilage degradation and chondrocytes death. Chondrocyte death is induced by the apotosis through special mechanisms including the activation of caspase-3. On the basis of this background, this study was designed to examine the cartilage protective and anti-apototic effects of Aralia Cordata in in vtro and in collagenase-induced arthritis rabbit model. To conduct in vitro study, chondrocytes culturedfrom rabbit knee joint were treated by 5 ng/ml IL-1a.For in vivo experiment, collagenase-induced arthritis (CIA) rabbit model was made via intraarticular injection with 0.25 ml of collagenase solution. Aralia cordata pharmacopuncture (ACP) was administrated on bilateral Dokbi acupoint (ST35) of rabbits at a dosage of 150 ${\mu}g/kg$ once a day for 28 days after the initiation of the CIA induction. In the study by using CIA rabbit model in vivo, ACP showed the inhibition of cartilage degradation in histological analysis. Aralia cordata also showed anti-apoptotic effect both in vitro and in vivo study. In chondrocytes treated by IL-1a, Aralia cordata inhibited caspase-3 activity and enhanced the proliferation of IL-1a-induced dedifferentiated chondrocytes. ACP showed the inhibition effect on the caspase-3 expression and activity from CIA rabbit model. This study indicates that ACP inhibits the cartilage destruction and the chondrocyte apotosis through downregulation of caspase-3 activity. These data suggest that ACP has a beneficial effect on preventing articular cartilage destruction in osteoarthrtis.

• 한국환경과학회지
• /
• 제29권10호
• /
• pp.997-1002
• /
• 2020

In this study, we investigated the effects of the eco-friendly chemical bio-sulfur, on the citrus melanose-causing pathogen, Diaporthe citri, and on the pest, Panonychus citri. In an open field experiment with a plot-scale application of the chemicals: Mancozeb, lime sulfur, lime sulfur + machine oil, and bio-sulfur, the control group showed 70.6% disease severity compared with 10.3% for the Mancozeb-treated group. Among the eco-friendly treatments, disease severity was the lowest for the group treated with lime sulfur + machine oil (32.2%) and was 53.9%, 58.8%, and 58.1% following treatment with lime sulfur, and bio-sulfur diluted 500 and 1000 times, respectively. The proportion of diseased fruit showed similar results, suggesting that bio-sulfur is an effective alternative to lime sulfur. Three days after treatment acaricidal effects on P. citri showed a 197.6% control survival rate whereas the machine oil, and bio-sulfur diluted 500 and 1000 times treatments showed rates of 2.9%, 5.8%, and 9.0%, respectively. After three days, the control value for bio-sulfur diluted 1000 times was 73.2% compared with the values for the machine oil (96.4%) and bio-sulfur diluted 500 times (94.6%) treatments. Therefore, we suggest that additional research is needed on the combined application of bio-sulfur and oils to enhance the additive control effect on citrus melanose and Panonychus citri.

• Journal of Plant Biology
• /
• 제17권1호
• /
• pp.15-21
• /
• 1974

The most commonly grown economical and flue-cured tobacco cultivar Yellow Special A was used in pot-culture tests in order to study Ethrel (2-chloroethyl phosponic acid) effects on accelerating maturity of tobacco leaves in relation to the most adequate level of the chemical useful for field growing, nitrogen level in soil for the most pronounced response, and the most suitable spray period during the growth stages of pre-, post- and topping periods. The following conclusions, thus, were obtained from the present studies; 1. 500ppm Ethrel spray was reconfirmed to be adequate in the practical applications, although the extent of yellow-ripening of tobacco leaves was increased as the Ethrel level increased. The highest leevel treated resulted in causing chemically damaged lesions on leaves and early defoliation. 2. Ethrel-treated leaves showed deeper yellowish tinge to them than those without treatment, while different levels of the chemcial had less influence on the tinge. 3. An adequate level of nitrogen supply to plants favored the Ethrel response, whereas either very low or high level of nitrogen in the soil lowered the chemical effect on accelerating the yellow-ripening. When carbohydrates versus total nitrogen ratio became relatively high, the condition brought out some outstanding Ethrel effects. 4. Chlorophyll level of leaves increased as soil applications of nitrogen level increased, and that also increased carotenoid level of the tobacco leaves. Ethrel-treated leaves showed deeper orange tinge than those without treatment, while the highest level of nitrogen application showed the deepest orange tinge to tobacco leaves. 5. Pre-topping treatment (12 days before topping and flowering) resulted in almost no Ethrel response, and that treatment right on the day of topping, showed response of yellow-leaf ripening at nearly bottom-half leaves of a tobacco plant. The post-topping treatment (12 days after topping) made plants showing full response of Ethrel from bottom to the top leaves of tobacco plant in accelerating the leaf maturity. 6. The extent of Ethrel responses on accelerating yellow-ripening of tobacco leaves was discussed for the modifying influences brought about by certain environmental factors. Discussions were also made about the possible practical applications (particularly for pre-rice planting) and quality difference that may be caused by such growth environments.

• 대한의료기공학회지
• /
• 제20권1호
• /
• pp.104-117
• /
• 2020

Objective : The purpose of this study is to report that Korean Medicine treatment is effective in treating pain in burn patients. Results : We effectively reduced the pain that occurred after the end of the burn treatment through Korean Medicine such as multiple acupuncture, cupping, Sugi therapy, and external salve. VAS(Visual Analog Scale) values were reduced from 8 to 2 after about 23 days of treatment. Conclusions : This report shows that there was no special effect on dermatology treatment for pain that occurred after the end of treatment of burn patient, but it can also be excellent through Korean Medicine alone. It's only one case, so it cannot be determined that Korean Medicine for a burn is excellent. but if more excellent burn treatment of Korean Medicine is reported continuously, it will prove its validity. Therefore, it is hoped that more case reports and studies on burn treatments will be done in the future.