• Restorative Dentistry and Endodontics
• /
• v.27 no.4
• /
• pp.421-431
• /
• 2002

C-shaped canal configuration is very difficult to treat because that clues about preoperative canal anatomy cannot be ascertained from clinical crown morphology and limited information can be derived from radiographic examination. This study was done to get more informations about the root and canal configuration of C-shape root by 3-dimensionally reconstructing for the purpose of enhancing success rate of endodontic treatment. 30 mandibular molars with C-shaped root were selected. Six photo images from occlusal, apical, mesial, distal, buccal, lingual directions and radiographic view were taken as preoperative ones to compare them with 3-D image. After crown reduction to the level of 1-2mm over pulpal floor was performed, teeth were stored in 5.25% sodium hypochlorite solution for the removal of pulp tissue and debris. They were cleaned under running water, allowed to bench dry and embedded in a self-curing resin. This resin block was serially ground with a microtome (Accutom-50, Struers, Denmark) and the image of each level was recorded by digital camera (FinePix S1-pro, Fuji Co., Japan). The thickness of each section was 0.25mm. Photographs of serial sections through all root canal were digitized using Adobe Photoshop 5.0 and then minimum thickness of open and closed sites were measured (open site is the surface containing occluso-apical groove closed site is oppsite). After dizitization using 3-D Doctor (Able software Corp, USA). 3D reconstruction of the outer surface of tooth and the inner surface of pulp space was made. Canal classsification of C-shaped roots was performed from this 3-D reconstructed image. The results were as follows : 1. Most C-shape rooted teeth showed lingual groove (28/30). 2 According to Vertuccis' calssification, type I, II, III, IV, VII were observed. but also new canal types suck as 2-3-2, 1-2-3-2. 2-3-2-1, 2-3-2-3 were shown. 3 There was little difference in minimum thickness on coronal and apical portions, but open site were thinner than closed site on mid portion. Conclusively, 3D reconstruction method could make the exact configurations of C-shape root possible to be visualized and analyzed from multi-directions. Data from minimum thickness recommend cleaning and shaping be more carefully done on dangerous mid portion.

• Korean Journal of Ecology and Environment
• /
• v.43 no.3
• /
• pp.409-417
• /
• 2010

Present study aimed to investigate morphological and physiological change in the tissues of Carassius auratus after exposure against Pb (lead) with various rearing condition. We measured the level of accumulated heavy metal and analyzed ultrastructure with transmission electron microscopy. The heavy metal, Pb, was accumulated in the gill, bone and integument increased drastically for exposure periods, the 40 days. The accumulation of Pb in the gill showed relatively higher than that in other tissues. And accumulated Pb amounts also were increased with exposure time dependent manner in the gill, bone and integument tissues. Also, specific activities of antioxidation enzymes in all tissue after exposure to Pb were increased in the course of exposure. And the activities of SOD from tissues exposed to Pb were increased 2 folds than those from the unexposed but GPX activities were maintained constant. The increased numbers of mucous cells in gill tissues exposed to Pb were determined and morphological changes, such as clubbing and fusion, were shown secondary lamella. Also, exposure of Pb for 40 days on gills tissues cause membrane damage in mitochondria and nucleus. In kidney tissues, the atrophied glomerulus was observed, and the empty space in Bowman's capsule was expanded. Based on the all results, it is suggested that the exposure to the high level of Pb for long period affect on the morphology of tissues, and change the enzymatic balance in C. auratus.

• Tuberculosis and Respiratory Diseases
• /
• v.53 no.5
• /
• pp.569-576
• /
• 2002

Background : Chlamydia pneumoniae is one of common causes in upper and lower respiratory infections. Isolating C. pneumoniae from clinical specimens is very difficult due to the characteristics of the organism. Recently, we succeeded in isolating C. pneumoniae from a Korean patient, who suffered from acute pharyngitis. This is the first isolate from a clinical specimen in Korea. Methods : We attained a nasopharyngeal swab from a 22-year-old female patient, and inoculated it on a monolayer of the Hep-2 cell line. After 8 passages, we found the inclusion bodies of C. pneumoniae by an immunofluorescence(IF) test. The species-specific monoclonal antibody IF staining and species-specific PCR were done to confirm the species of the isolate, and electron microscopy was used to characterize the morphology. Results : The isolated was confirmed to be C. pneumoniae by species-specific IF and PCR, and the strain was named LKK-1. The shape of the elementary body was round and with a narrow periplasmic space, as shown by electron microscopy, which is similar to the Japanese strain, but not the Western strain. Conclusion : We succeeded in isolating C. pneumoniae from a 22-year-old patient with acute pharyngitis, which is the first isolate in Korea. In the future, this Korean strain will be useful to the study of C. pneumoniae.

• Journal of the Institute of Electronics Engineers of Korea CI
• /
• v.43 no.4 s.310
• /
• pp.58-66
• /
• 2006

In this paper, we apply DCM(Dilation of Color and Motion information) mask and Active Contour Models(Snakes) to extract facial outline in moving pictures with complex background. First, we propose DCM mask which is made by applying morphology dilation and AND operation to combine facial color and motion information, and use this mask to detect facial region without complex background and to remove noise in image energy. Also, initial curves are automatically set according to rotational degree estimated with geometric ratio of facial elements to overcome the demerit of Active Contour Models which is sensitive to initial curves. And edge intensity and brightness are both used as image energy of snakes to extract contour at parts with weak edges. For experiments, we acquired total 480 frames with various head-poses of sixteen persons with both eyes shown by taking pictures in inner space and also by capturing broadcasting images. As a result, it showed that more elaborate facial contour is extracted at average processing time of 0.28 seconds when using interpolated initial curves according to facial rotation degree and using combined image energy of edge intensity and brightness.

• Journal of the Korean Electrochemical Society
• /
• v.11 no.4
• /
• pp.268-272
• /
• 2008

$LiFePO_4$ material was synthesized by electrospinning method to obtain optimal particle size($50{\sim}100\;nm$) without carbon coating or ball milling. This material showed an orthorthombic structure with Pnma space group without any impurities, such as FeP or $Fe_2P$, in the XRD pattern. The particle morphology and particle shape were observed by SEM analysis. Li/$LiFePO_4$ cell showed a high initial discharge capacity of 135 mAh/g, at current density of $0.1\;mA/cm^2$ with a cut-off voltage of 2.8 to 4.0V. This cell exhibited a perfect cycle performance over 99.9% cycle retention rate up to 50 cycles.

• Spatial Information Research
• /
• v.18 no.4
• /
• pp.1-11
• /
• 2010

Unlike the previous studies which have focused on 2-dimensional urban characteristics, this paper presents statistical models explaining urban heat island(UHI) effect by 3-dimensional urban morphologic information and addresses its policy implications. 3~dimensional informations of Columbus, Ohio arc captured from LiDAR data and building boundary informations are extracted from a building digital map, Finally NDV[ and temperature data are calculated by manipulating band 3, band 4, and thermal hand of LandSat images. Through complicated data processing, 6 independent variables(building surface area, building volume, height to width ratio, porosity, plan surface area) are introduced in simple and multiple linear regression models. The regression models are specified by Box-Tidwell method, finding the power to which the independent variable needs to raised to be in a linearity. Porosity, NDVI, and building surface area are carefully chosen as explanatory variables in the final multiple regression model, which explaining about 57% of the variability in temperatures. On reducing UHI, various implications of the results give guidelines to policy-making in open space, roof garden, and vertical garden management.

• Journal of the Korea Computer Graphics Society
• /
• v.24 no.1
• /
• pp.9-16
• /
• 2018

In this paper, we introduce a visualization framework for cell image data obtained from optical diffraction tomography (ODT), including a method for representing cell morphology in 3D virtual environment and a color mapping protocol. Unlike commonly known volume data sets, such as CT images of human organ or industrial machinery, that have solid structural information, the cell image data have rather vague information with much morphological variations on the boundaries. Therefore, it is difficult to come up with consistent representation of cell structure for visualization results. To obtain desired visual representation of cellular structures, we propose an interactive visualization technique for the ODT data. In visualization of 3D shape of the cell, we adopt a volume rendering technique which is generally applied to volume data visualization and improve the quality of volume rendering result by using empty space jittering method. Furthermore, we provide a layer-based independent rendering method for multiple transfer functions to represent two or more cellular structures in unified render window. In the experiment, we examined effectiveness of proposed method by visualizing various type of the cell obtained from the microscope which can capture ODT image and fluorescence image together.

• Journal of Korea Water Resources Association
• /
• v.42 no.5
• /
• pp.397-406
• /
• 2009

River is able to change by various environmental factors. In order to conduct restoration design of abandoned river channels, it is necessary to evaluate the river through the analysis of past and present river channels. River evaluation requires various data, such as geometry, hydraulic and hydrology, but there is a lot of difficulty to understand topographical information of river change on time and space due to a lack of past data by domestic conditions. This study analyzes the changes in past and present river channels and examines the applicability of river channel evaluation through image analysis using aerial photographs and 1918 year's map. Aerial photograph analysis was conducted by applying the image analysis method and GIS analysis method on Cheongmicheon. As a result of this analysis, we have quantitatively identified the form and size of abandoned channels, changes in the vertical-section and cross-section length of rivers, and micro-landform changes. More importantly, we verified that morphological changes in sandbars due to artificial straightening are important data in identifying the state of current river channels. In these results, although image analysis technique has limitations in two-dimensional information from aerial photographs, we were able to evaluate the changes in river channel morphology after artificial maintenance of the river.

• Korean Journal of Animal Reproduction
• /
• v.25 no.4
• /
• pp.399-407
• /
• 2001

The microinjection of retroviral vectors into the perivitelline spaces of MII-stage oocytes increased production of transgenic bovine embryos. However, oocytes have various sizes of perivitelline space, and there is the tendency that the oocyte membranes are damageable by micropipettes during the injection of retroviral vectors into perivitelline spaces or oocytes. Thus, it was not always possible to stably inject retroviral vector into perivitelline spaces of oocytes. Here we used sucrose to minimize the damage of the oocyte membrane. When the oocytes were suspended in 0.5% sucrose, poor quality oocytes showed rough cytoplasmic membranes, while good quality oocytes maintained smooth membranes. However, when the tatters were subjected to in vitro fertilization, no significant differences were observed in cleavage rates (82% of control Vs. 84% of sucrose treated oocytes). The Same trends were obtained from the oocytes fertilized after microinjection of LN$\beta$-EGFP and LNC-hGH genes into the perivitelline spares. The rates of cleavage and blastocyst from microinjection of LN$\beta$-EGFP genes were 81 and 25%, and from microinjection of LNC-hGM genes were 53 and 30%, respectively. The result indicated that microinjected oocytes could develop to the blastocyst stages after in vitro fertilization with no significant difference from control group. Moreover, the integration of hGH-gene (by PCR analysis) was detected in 52% of infected cleaved embryos and the expression of EGFP-gene (under a fluoresrence microscope) was also observed in 34% of infected blastocyst. These results indicated that 0.5% sucrose treatment could be an efficient method not only to select good quality embryos but also to inject retroviral vectors into perivitelline spares without any harm and hence improving developmental rates.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.17 no.3
• /
• pp.380-389
• /
• 2007

In Korea, undoubtedly, the cancer is one of the most common reasons of death, and hepatoma is the second highest fatal cancer regardless of the gender only next to the stomach cancer In the middle and prime-aged between 40 and 60 years, the incidence of hepatoma is the highest in the world, and the death rate due to hepatoma is the highest among OECD countries. In this paper, we propose a novel method for automatic identification of hepatoma from a contrast enhanced CT images, which is used in an expert system that helps medical specialists. First, consecutive $40{\sim}50$ contrail enhanced CT images are photographed by every 5mm from the upper part of the chest, and using position information on the rib, we classify the internal area including only internal organs and the external one that consists of the rib, subcutaneous fat layers, and the background from the CT images. Then, the region of the liver is extracted from the classified internal area by using information on the intensity, the distribution of brightness, and using the regions extracted from consecutive images, we restore information on the 5 mm space occurred between the consecutive two slides tty applying a shape-based interpolation method. Lastly, using the characteristics such as the brightness and the morphology, we are able to extract the regions of hepatoma. The expert system based on our method is sufficiently competitive when it is compared with the diagnoses by specialists in the diagnostic radiology.