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Four New Species of the Genus Hyattella (Dictyoceratida: Spongiidae) from Korea

  • Sim, Chung Ja;Lee, Kyung Jin
    • Animal Systematics, Evolution and Diversity
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    • v.30 no.1
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    • pp.16-21
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    • 2014
  • Four new species of the genus Hyattella (Dictyoceratida: Spongiidae); H. sinchangensis n. sp., H. mara n. sp., H. chuja n. sp. and H. lendenfeldi n. sp., were collected from Jeollanam-do and Jeju-do, Korea. Hyattella sinchangensis n. sp., H. mara n. sp., H. chuja n. sp. and H. grobosa Lendenfeld, 1889 are very similar to each other in some characters. However, Hyattella sinchangensis n. sp. characterized by no special dermal lamella and no tertiary fibres, and more thin secondary fibres than H. globosa. Hyattella mara n. sp. is differs in having well developed dermal lamella, slightly fasciculated primary fibres, and thick tertiary fibres. Hyattella chuja n. sp. differs in surface characters with a slightly elevated part, skeleton with abundant spongine, and very complex meshes. Hyattella lendenfeldi n. sp. is similar to H. tenella (Lendenfeld, 1889) in skeletal structure, thickness of primary and secondary fibres, but differs in growth form.

The distribution of protozoans according to soil pollution around Ulsan industrial complexes, Korea (울산공단 주변의 토양오염에 따른 원생동물의 분포)

  • 신만균;최기룡
    • Journal of Environmental Science International
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    • v.5 no.2
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    • pp.187-194
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    • 1996
  • The present study was performed to elucidate the distribution of protozoans according to the actual conditions of soil pollution around Ulsan industrial complexes, Korea. Samples were collected from the top-soils of 13 localities in eight times during the period from 16 April 1994 to 14 January 1995. As a result of this study, total 11 species of hypotrichous ciliated protozoa were identified and analyzed. These hypotrichs are 6 species of stichotrichine hyporichs ( Keronopsis sp., Pseudourostyla sp., Hoiriiticha syivatica, Hipiosticha multistylata, Hoios-ticha sp. and ParuroleFitus sp.) and 5 species of sporadotrichine hypotrichs (Oxyricha sp., Steinla sp., Histriculus cavicola, Hemisincirra sp. and Gonostomum auiili). Of these 11 species, 4 species (Keronopsis sp., Pseudourostyla sp., Holosticha sp. and Hemisincirra sp.) are reriorted for 1%e first time from Korea. All the sampling localities could be grouped in three zones by the cluster analysis with the abundance and distribution of protozoans. This result is approximately coincide with the ionation by the concentration of heavy metals.

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Bacteriocin Production by Streptococcus sp. J-C1 Isolated from Kimchi (김치에서 분리한 Streptococcus sp. J-C1의 bacteriocin 생산)

  • Jo, Young-Bae;Cho, Young-Im;Baik, Hyung-Suk;Jun, Hong-Ki
    • Journal of Life Science
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    • v.6 no.4
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    • pp.270-277
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    • 1996
  • Streptococcus sp J-C1 producing bacteriocin was isolated from Kimchi. The optimum conditions for bacteriocin production by Streptococcus sp. J-C1 were evaluated. For the maximum yield of bacteriocin production by Streptococcus sp. J-C1, the cell should be harvested at the late stationary phase and the temperature, pH and NaCl concentration should be 25$\circ$C, pH 8 and without the addition of NaCl, respectively. Sucrose should be used as a carbon source and organic nitrogen such as peptone should be used as a nitorgen source for the best yield. The production of bacteriocin is related to the cell growth of Streptococcus sp. J-C1. The bacteriocin from Streptococcus sp. J-C1 was active for gram positive microorganisms such as Lactobacillus sp., Leuconoctoc sp., Lactococcus sp., Streptococcus mutans, Staphylococcus aureus amd Bacillus subtilis and also active for gram negetive bacteria such as Acetobacter aceti. Antibacterial activity of the bacteriocin was completely disappeared by protease treatment.

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Biocontrol of Cabbage Clubroot by the Organic Fertilizer Using Streptomyces sp. AC-3. (Streptomyces sp. AC-3을 이용한 배추 무사마귀병의 생물학적 방제)

  • 주길재;김영목;김정웅;김원찬;이인구;최용화;김진호
    • Microbiology and Biotechnology Letters
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    • v.32 no.2
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    • pp.172-178
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    • 2004
  • This research is performed for a biological control of Chinese cabbage clubroot, we isolated an antagonistic bacterium AC-3 against Plasmodiophora sp., causal pathogens of cabbage clubroot. The isolated strain was identified as Streptomyces sp. by culture morphology, biochemical reactions, and homology research based on l6S rDNA sequences. Streptomyces sp. AC-3 produced chitinase (9.3 units/$m\ell$) in culture broth. So Plasmodiophora sp. mycelia changed abnonnal swelling, curling and branching mycelia by Streptomyces sp. AC-3 culture. In a field infected by Plasmodiophora sp., the treatment of a organic fertilizer added 2% Streptomyces sp. AC-3 microbial inoculant, it resulted in about 50% reducing the severity of cabbage clubroot significantly on cabbage plants compared with treated organic fertilizer plants. Additional disease such as sclerotinia rot, fusarium wilt and pythium rot were also significantly reduced by the treatment of the organic fertilizer added Streptomyces sp. AC-3 microbial inoculant.

Sphingobacterium sp. SW-09 Effectively Degrades Phenanthrene, a Polycyclic Aromatic Hydrocarbon, in a Soil Microcosm (Sphingobacterium sp. SW-09에 의한 토양환경에서의 다환 방향족탄화수소인 페난스렌의 분해)

  • Son, Seung-Woo;Chang, Hey-Won;Kim, Sung-Kuk;Chang, Jong-Soo
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1511-1517
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    • 2011
  • We isolated a potent phenanthrene-degrading bacterium from oil-contaminated soils of Suzhou, China, and assessed the potential use of these bacteria for bioremediation of soils contaminated by polycyclic aromatic hydrocarbons (PAHs) in a microcosm. Based on 16S rDNA sequencing, we identified this bacteria as Sphigobacterium sp. SW-09. By PCR amplification, we also identified catechol 2,3-dioxygenase genes (nahH genes) mediating PAH degradation. Staphylococcus sp. KW-07, which has been identified in our previous study, showed potential for use in bioremediation of oil-contaminated soils. In this experiment, we compared the rate of phenanthrene-degradation between Staphylococcus sp. KW-07 and Sphingobacterium sp. SW-09 in a microcosm condition. Newly isolated Sphingobacterium sp. SW-09 showed a higher phenanthrene-degradation rate than that of Staphylococcus sp. KW-07 in soil microcosms. Together, our results suggest that the Sphingobacterim sp. SW-09 strain isolated from the Suzhou area may also be useful in bioremediation of PAH-contaminated soils.

Improvement of 4-chlorobiphenyl degradation bya recombinant strain, pseudomonas sp. DJ12-C

  • Kim, Ji-Young;Kim, Young-Chang;You, Lim-Jai;Lee, Ki-Sung;Ok, Ka-Jong;Hee, Min-Kyung;Kim, Chi-Kyung
    • Journal of Microbiology
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    • v.35 no.1
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    • pp.53-60
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    • 1997
  • Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 isolated from the polluted environment are capable of degrading biphenyl and 4-chlorobiphenyl (4CB) to produce benzoic acid and 4-chlorobenzoic acid (4CBA) respectively, by pcbABCD-encoded enzymes. 4CBA can be further degraded by Pseudomonas sp. DJ-12, but not by Pseudomonas sp P20. However, the meta-cleavage activities of 2, 3-dihydroxybiphenyl (2, 3-DHBP) and 4-chloro-2, 3-DHBP dioxygenases (2, 3-DHBD) encoded by pcbC in Pseudomonas sp. P20 were stronger than Pseudomonas sp. DJ-12. In this study, the pcbC gene encoding 2, 3-DHBD was cloned from the genomic DNA of Pseudomonas sp. P20 by using pKT230. A hybrid plasmid pKK1 was constructed and E. coli KK1 transformant was selected by transforming the pKK1 hybrid plasmid carrying pcbC into E. coli XL1-Blue. By transferring the pKK1 plasmide of E. coli KK1 into Pseudomonas sp. DJ-12 by conjugation, a recombinant strain Pseudomonas sp. P20, Pseudomonas sp. DJ-12, and the recombinant cell assay methods. Pseudomonas sp. DJ12-C readily degraded 4CB and 2, 3-DHBP to produce 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA), and the resulting 4CBA and benzoic acid were continuously catabolized. Pseudomonas sp. DJ12-C degraded 1 mM 4CB completely after incubation for 20 h, but Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 showed only 90% and Pseudomonas sp. DJ-12 had, but its degradation activity to 2, 3-DHBP, 3-methylcatechol, and catechol was improved.

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Effects of Deodeok Contents on the Qualities of Quick Fermented Doenjang Type Product (더덕을 첨가하여 속성시킨 된장형 제품의 품질에 미치는 영향)

  • Hong, Seong-Cheol;Choi, Ki-Soon;Lee, Ho-Joon;Kwon, Dong-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.5
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    • pp.757-763
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    • 2010
  • The qualities of Deodeok Doenjang including physicochemical characteristics and sensory properties were investigated over the following range of Deodeok levels: 5, 10, 15 and 20% (all w/w). The strains used in the manufacturing of Doenjang were Bacillus sp. B-3 and Aspergillus sp. M-9 with the highest enzyme activities like amylase and protease. In case of Deodeok Doenjangs prepared with Bacillus sp. B-3, the amino-type nitrogen of Doenjang containing 10% (w/w) Deodeok was produced more than others during fermentation for 40 days. Amino-type nitrogen produced in Deodeok Doenjang prepared with Aspergillus sp. M-9 was more than one of Deodeok Doenjangs with Bacillus sp. B-3 and a commercial Doenjang. When Deodeok content exceeded 15% (w/w), higher content resulted in lesser amino-type nitrogen production. The results showed that Deodeok had influenced growth of Bacillus sp. and Aspergillus sp. Sensory evaluation showed that Deodeok Doenjangs containing 10% (w/w) Deodeok and 1% (w/w) Bacillus sp. B-3 and containing 20% (w/w) Deodeok and 1% (w/w) Aspergillus sp. M-9 were superior to the other Doenjangs tested.

A Study to Establish the Core Evaluation Items of SP Authentication using ANP(Analytic Network Process) (ANP 모형을 이용한 SP 인증 핵심 평가항목 도출 연구)

  • Huh, Sang Moo;Kim, Woo Je
    • Journal of Information Technology Services
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    • v.17 no.1
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    • pp.59-77
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    • 2018
  • Software process (SP) authentication is a certification system designed in order to improve software process and ensure software reliability of the Korean small and medium enterprises (SMEs). It is composed of 3 grades, 17 evaluation items and 70 detailed evaluation items (software process). In order to certificate grade 2, 42 detailed evaluation items must be satisfied. In order to certificate grade 3, all 70 detailed evaluation items must be satisfied. SMEs are more vulnerable to manpower and material resources than the major company. Therefore, it is difficult for SMEs to invest in the long term to improve the software process. If we can provide the core evaluation items to SMEs, SMEs will be able to improve software quality more efficiently and effectively. It can be used as a short way to acquire SP authentication. The evaluation items of SP are defined as influencing each other. Therefore, the core evaluation items might be established using related techniques. Although we searched for previous researches and related informations, the associated study was insufficient. In this study, ANP (Analytic Network Process) technique was applied, and the core evaluation items of SP authentication were established. SMEs will be able to acquire SP authentication more easily using the core evaluation items. Finally, we expect that this study will be used as a basis material for creating the SP 2.0 version.

Isolation and Characterization of White Rot Fungi for Decolorization of Several Synthetic Dyes (염료의 색도 제거능력이 우수한 백색부후균 분리 및 특성연구)

  • 오광근;김현수;조무환;채영규;전영중
    • Microbiology and Biotechnology Letters
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    • v.27 no.6
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    • pp.500-508
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    • 1999
  • Several white-rot fungi collected from the mountains of Korea were evaluated for their ability to decolorize azo, polymeric, and reactive dyes. Strains CJ-105, CJ-212 and CJ-315, identified as Trametes sp., Pleurotus sp. and Fomes sp., respectively, showed higher potential for decolorization of those dyes in either solid or liquid media. For Trametes sp. CJ-105, 100ppm of Remazol Brilliant blue R and 500ppm of Acid Red 264 were completely decolorized after 2 days under liquid culture. The dominating ligninolytic enzyme existing in the culture broth was laccase (E.C. 1.10.3.2). Also, Pleurotus sp. CJ-212 and Fomes sp. CJ-315 showed similar patterns in decolorization of Remazol Brilliant Blue R and Acid Red 264. The extent of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic enzymes produced by each strain. In addition to that Trametes sp. CJ-105 was highly effective in degradation of polycyclic aromatic hydrocarbons and pentachlorophenol by the activity of the ligninolytic enzymes produced. In this study, we found that white-rot fungi, Trametes sp. CJ-105(KFCC 10941), Pleurotus sp. CJ-212(KFCC 10943) and Fomes sp. CJ-315(KFCC 10942), were effective in decolorizing a wide range of structurally different synthetic dyes, as well as some chemical compounds which are known to be hardly degradable.

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