• 한국식품과학회지
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• 제44권5호
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• pp.600-606
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• 2012

콩을 소재로한 전통 발효식품으로부터 혈전용해능이 뛰어난 균주를 분리하였으며, B. subtilis로 동정되었다. 따라서 이를 B. subtilis IDCC 9204(특허균주기탁: KCTC-11471 BP), 그 혈전용해 효소는 NK-IL9204로 명명하였다. B. subtilis IDCC 9204가 생산하는 고역가의 NK-IL9204를 단백질 분석법에 기초하여 분석한 결과, 분자량은 27.7 kDa의 homogenous enzyme으로 확인되었다. 또한 기존에 알려진 일본의 발효식품인 낫도 유래의 B. subtilis var. natto가 생산하는 nattokinase 와의 sequence 분석을 진행한 결과, 99.5% homology가 일치하는 serine protease계열의 nattokinase로 확인되었다. 그러나 NK-IL9204는 물리 화학적인 조건에서 B. subtilis var. natto가 생산하는 nattokinase와 다소 차이를 나타내었으며 본 실험에서는 B. subtilis var. natto가 생산하는 nattokinase보다 상대적으로 높은 열 안정성과 pH 안정성을 나타내었다. In vitro 실험에서 NK-IL9204는 최적 반응온도 $40^{\circ}C$, 열 안정성은 $90^{\circ}C$까지 효소활성을 유지하였으며, 최적 반응 pH는 pH 8로 알칼리-혈전용해효소의 특성을 나타내었으며, 약산성에서 강알칼리 영역까지 넓은 pH 구간 안정성을 갖는 것이 특징이다. NKIL9204의 in vivo에서의 효능과 생체 내 안정성을 동물실험을 통해 확인한 결과, 생체 내에서도 혈전용해효소의 활성이 소실되지 않고 유지되며, 혈전분해와 관련된 생체 내 인자들을 활성화시키는 역할을 하는 특징을 갖는다. NK-IL9204는 30,000 FU/g 이상의 고역가를 달성하여 산업적 측면에서 생산성도 확보함으로써 수입의존적 원료를 국산화할 수 있을 것으로 예상된다.

• 생명과학회지
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• 제20권12호
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• pp.1872-1881
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• 2010

본 연구에서는 대두(FS)와 아가콩의 발효추출물(FYA)의 항암활성 기전을 확인하기 위해 AGS 인체위암세포의 증식에 미치는 영향을 조사하였다. AGS 세포에서 FS 및 FYA 처리로 인하여 암세포의 증식이 처리 농도 의존적으로 강하게 억제하였고, apoptosis 유발을 의미하는 세포의 전반적인 형태 및 핵의 변형 또한 동반하였다. 또한 세포주기 분석을 통하여 이 현상이 apoptosis 유도에 의한 것임을 확인하였다. AGS 세포에 처리된 FS 및 FYA는 pro-apoptotic factor인 Bax의 발현 증가를 통한 intrinsic pathway나, death receptor 관련 유전자의 발현 증가를 통한 extrinsic pathway를 활성화시키며, 더 나아가서 IAP family인자의 발현 억제 및 caspases의 활성 증가를 일으켜 apoptosis를 유발시키는 것을 유추할 수 있었는데, 이러한 효과들은 FS보다 FYA에서 더욱더 탁월하였다. 이는 향후 아가콩 발효추출물이 항암치료를 위한 적용 가능성이 매우 우수함을 제시하여 주는 결과이다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1149-1156
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• 2001

Previous studies conducted with swine have reported that the mobile nylon bag technique (MNBT) does not always accurately predict in vivo nutrient digestibilities. Therefore, in this study, the MNBT was modified so that nutrient digestibilities would more closely resemble those from conventional (Con) digestibility studies obtained using the indicator method. A total of 19 feeds were tested including five cereal grains, five legumes, three high protein sources and six mixed diets. The principle changes to the MNBT included the use of a fecal collection harness which minimized the number of bags lost. In addition, previous protocols involved pooling of bags within pig while in the present experiment all bags were analyzed separately to increase the precision of the test. Finally, chemical analyses were done using the entire nylon bag plus residue rather than opening.the bags and scraping out the contents. With the exception of the barley sample (p=0.01), dry matter digestibility (DMD) coefficients obtained with the MNBT were not significantly different from those obtained with the indicator method. The linear regression equation relating the MNBT to the indicator method was Con DMD=-O.77+1.02 MNBT DMD ($r^2=0.93$: p<0.0001). There was no significant (p>0.05) difference in gross energy digestibility (GED) coefficients determined using the MNBT or the indicator method for any of the 19 feeds. The regression line equation relating the MNBT to the indicator method was Con GED=-5.68+1.06 MNBT GED ($r^2=0.94$: p<0.0001). The MNBT was less effective in predicting in vivo crude protein digestibility (CPD) than it was in predicting dry matter and energy digestibility. Differences greater than five percentage units were observed for two of the legumes, Kabuli chickpeas (p=0.02) and the extruded pea-canola seed mixture (p=0.01) as well as for three of the mixed diets including the unheated hulled barley-based diet (p=0.01), the unheated hulless-barley based diet (p=0.08) and the barley-soybean meal based diet (p=0.008). The regression equation relating the MNBT to the indicator method was Con CPD=5.75 + 0.90 MNBT CPO ($r^2=0.76$; p<0.0001). This study indicates that the modified MNBT can be used for the rapid determination of dry matter and energy digestibility in a wide variety of ingredients. For the measurement of crude protein digestibility, the technique produces results similar to conventional digestibility studies for cereal grains and high protein feeds but tends to overestimate protein digestibility for legumes and mixed diets.

• 한국지역사회생활과학회지
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• 제17권1호
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• pp.141-154
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• 2006

The purpose of this study is to provide the information of the aged olds for which basic data are almost no available. The subjects were divided on the base of age 75. The elderly under 75 were named 'the young olds' and over 75 were 'the aged olds'. The aim of this research is to promote health and to improve nutrition, and the survey was made for health promotion behaviors, habits against health risk, dietary management status and diet intake. And it was conducted by 24 hr-recall method and analyzed by nutrients intake. 242 subjects were collected in 12 cities or counties in Gyeonggi Province and 20 elderly people (10 male and 10 female) were selected out of 1 village in each district. Questionnaire about health behavior and dietary management was carried out by interview method through regional home extension workers. Statistical analyses were made by SAS (version 8.1) and Chi-square tests and General Linear Models were used. Out of the subjects the aged olds over 75 was 31.9%, elementary school educated (93.5%), with spouse (40.3%), with adult children(28.6%), monthly living cost of 500-1,000 thousand won(40.3%). Mean age was 78.82 years compared with 69.75 years of the young olds. 46.8% of the aged olds used monthly pocket money over 1000 won and it was lower than 63.3% of the young olds. Only half of the aged olds had regular exercise of walking (77.8%) or with athletic equipment (17.8%). However, the young olds did more frequent walking (82.1 %) and less exercise with athletic equipment (4.8 %), which was significantly different. Kinds of disease were different with the young or the aged olds, as more proportion of cardiovascular disease(37.9%) for the young olds and joint lumbago neuralgia(41.6%) for the aged olds. Dietary management was good (3 meals per day: 93.4%, fixed mealtime: 72.4%, and regular amount: 79.9%). But there was significant difference in side dish varieties and kinds of snacks; for the aged olds only 8% had over 5 sorts (compared with 18.8% of the young olds) and the kinds of snacks were cookie, candy, juice, carbonated beverage for the aged olds (compared with noodle, milk, soybean-milk for young olds). The ratio of nutrients intake (energy, riboflavin and niacin) with RDA was significantly higher for the aged olds than that of the young olds. The surveyed subjects had no difficulties in Activities of Daily Living (ADL), but some of the aged olds had difficulties in Instrumental Activities of Daily Living (IADL) like housekeeping, using transportation, going shopping and making phone calls. These results suggest that low quality of life is linked with low economic status of the rural elderly and congregate meal at village hall would be required because of the lack of side dishes variety for the aged olds. And nutrition education program about good snacks and exercise practice would be needed for the aged olds. By operating nutrition education program the aged olds would enjoy better quality life maintaining or ameliorating IADL abilities.

• 한국식생활문화학회지
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• 제16권4호
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• pp.341-353
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• 2001

This study was conducted to investigate dietary patterns and assimilation of dietary culture of Korean descendants in Yanbian, China compared to Korean. A dietary survey was conducted using one 24-hour recall method from 730 adults over 30 years of age in Yanbian, China and 695 adults over 25 years of age in Kuri, Korea. The average number of food items consumed per day was 14 in Yanbian and 20 in Kuri and there was a significant difference between the two regions(p<0.001). The foods consumed most frequently were rice, salt, the M.S.G. in Yanbian and rice, green onion and garlic in Kuri. The foods consumed most by amount were rice, cucumber, Soju in Yanbian and rice, Kimchi and grape in Kuri. The average number of dishes consumed per day was 6.4 in Yanbian, 9.4 in Kuri and the difference between the two regions was significant(p<0.001). Total number of dishes appeared in the survey was 253 in Yanbian and 494 in Kuri. The dishes consumed most frequently were cooked-rice, beverages, Kimchi, stir-fried eggplant in Yanbian and cooked-rice, Kimchi, coffee, soybean paste stew, in Kuri. The dish groups appeared most frequently were rice, stir-fried foods, vegetables and Kimchi in Yanbian and rice, Kimchi, beverages and fruits in Kuri. Male subjects in Yanbian Consumed alcohol frequently while adults in Kuri consumed coffee more frequently than Yanbian. The major meal patterns were rice+side dish, rice+soup, rice+side dish+Kimchi in Yanbian and rice+soup+side dish+Kimchi, rice+side dish+Kimchi, rice+soup+Kimchi in Kuri. Stir-fried foods, which were consumed 46.6% of all the meals, were dominant side dish in Yanbian, but stir-fried food, seasoned vegetables and grilled food appeared aver 10% in Kuri. There results show that variety of diet of Korean-Chinese in Yanbian was lower than Kuri. Korean-Chinese tend to keep traditional Korean dietary patterns of consuming rice as staple but were adapted to Chinese dietary patterns of consuming stir-fried side dishes. Efforts should be directed toward preserving Korean traditional patterns of dietary culture among Korean-Chinese population.

• 대한지역사회영양학회지
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• 제5권2호
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• pp.297-306
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• 2000

In order to examine the relationship between the number of different foods consumed and nutrient intake, one-day food consumption were surveyed by 24-hour recall from a sample of 287 individuals(20-49 years) living in Daejon City. The number of consumed food items did not include seasonings except red pepper power, sugar, oil, and soybean paste when used in large amounts(DVS). The number, including all seasonings except salt and vinegar(DVSS), was also counted. Of the total subjects, 43.6%(DVS) or 39.0%(DVSS) consumed 18-23 daily different foods with an average of 20.2 or 22.9, respectively. As the DVS increased, daily intakes of total foods and most food groups were elevated. Cereals however were not changed and eggs were decreased with increasing DVS. Energy and nutrient intakes and their mean adequacy ratio(MAR) and index of nutritional quality(INQ) also show positive correlations with DVS. MAR equal to or greater than($\geq$) 0.75 was taken as a cut-off point for nutritional adequacy. In that case, the mean INQ was shown to be $\geq$1. MRI(10) for energy and 9 nutrients and MAR(3) for Ca, vitamin A, and riboflavin were estimated. Form a regression analysis, when MAR(10) was 0.75, the DVS and DVSS were assumed to be 19.6 and 22.2, respectively. And when MAR(3) was 0.75, the DVS and DVSS were assumed to be 31.6 and 34.6, respectively. However the subjects whose MAR(3) was 0.75(0.7-0.8) 23.8(DVS) or 26.6(DVSS) different foods in average, and their mean intakes of energy and all nutrients, except vitamin A, were ranged at 85-100% of the RDA. Of the 74 subjects who consumed DVS$\geq$24, 24 to 42 numbers took 〈75% RDA of Fe, Ca, riboflavin, and vitamin A. And five of 7 who consumed DVS$\geq$32 took $\geq$125%RDA of protein, which showed concerns of overnutrition in case of DVS$\geq$32. Form the above results it could be suggested that a daily intake of 24-32(or 28) of DVS or 27-35(or 31) of DVSS was recommendable for an optimal nutritional of all nutrients if the variety of food groups and sufficient intake of vitamin A and calcium were emphasized together.

• 한국식생활문화학회지
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• 제34권5호
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• pp.508-533
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• 2019

This study investigated the Sachanbalgi, which record the royal feasts given by the royal family of the Joseon Dynasty of Korea. These records are contained within the Gungjung Balgi, which recorded the types and quantity of items used in royal court ceremonies. The Eumsikbalgi is the general name for the records of food found within this document. Using these Eumsikbalgi, and in particular the Sachanbalgi, this study investigated the food eaten and bestowed by the Joseon royal family. The Sachanbalgi describes four categories or occasions of feasts: royal birthdays, childbirth, royal weddings, and funerals. These records allow us to reconstruct who the attendees were and what the table settings and food were for instances not directly indicated in oral records, books, or other documents. The food at these Sachan (feasts) was diverse, being related to the specific event, and its contents varied based on the position of the person who was receiving the food. Usually, Bab (rice) was not found at a Sachanbalgi, and only on two occasions were meals with Bab observed. Specifically, it was served with Gwaktang (seaweed soup) at a childbirth feast. There were seven kinds of soups and stews that appeared in the Sachanbalgi: Gwaktang, Yeonpo (octopus soup), Japtang (mixed food stew), Chogyetang (chilled chicken soup), Sinseonro (royal hot pot), and Yukjang (beef and soybean paste). Nureumjeok (grilled brochette) and Saengchijeok (pheasant), and Ganjeonyueo (pan-fried cow liver fillet) and Saengseonjeonyueo (pan-fried fish fillet) were eaten. Yangjeonyueo, Haejeon, Tigakjeon (pan-fried kelp) and other dishes, known and unknown, were also recorded. Boiled meat slices appeared at high frequency (40 times) in the records; likewise, 22 kinds of rice cake and traditional sweets were frequently served at feasts. Five kinds of non-alcoholic beverages were provided. Seasonal fruits and nuts, such as fresh pear or fresh chestnut, are thought to have been served following the event. In addition, a variety of dishes including salted dry fish, boiled dish, kimchi, fruit preserved in honey, seasoned vegetables, mustard seeds, fish, porridge, fillet, steamed dishes, stir-fried dishes, vegetable wraps, fruit preserved in sugar, and jellied foods were given to guests, and noodles appear 16 times in the records. Courtiers were given Banhap, Tanghap, Myeonhap, wooden bowls, or lunchboxes. The types of food provided at royal events tracked the season. In addition, considering that for feasts food of the royal household was set out for receptions of guests, cooking instructions for the food in the lunchbox-type feasts followed the cooking instructions used in the royal kitchen at the given time. Previous studies on royal cuisine have dealt mostly with the Jineosang presented to the king, but in the Sachanbalgi, the food given by the royal family to its relatives, retainers, and attendants is recorded. The study of this document is important because it extends the knowledge regarding the food of the royal families of the Joseon Dynasty. The analysis of Sachanbalgi and the results of empirical research conducted to reconstruct the precise nature of that food will improve modern knowledge of royal cuisine.

• 한국작물학회지
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• 제35권4호
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• pp.342-351
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• 1990

하대두 채종시 문제가 되는 발아율 저하요인을 검토하기 위해 1987년 천안재래를 공시하여 평야지인 춘천(해발 74m)과 고랭지인 진부(해발 600m)에서 4월 25일부터 20일 간격으로 4회 파종하였고, 개화후 35일부터 5일 간격으로 5회의 수확시기를 두어 시험을 실시한 결과를 요약하면 다음과 같다. 1. 영양생장기간은 조기 파종의 경우와 고랭지인 진부에서 길었고 경장도 같은 경향이었다. 2. 본당협수 및 백립중은 고랭지인 진부에서 높은 수치를 보였으며 한파할수록 그리고 수확시기가 늦어질수록 큰 값을 보였다. 3. 종실수량도 진부가 춘천보다 많았고 한파할수록 많아지는 경향이었는데 진부에서 파종기간 수량차이가 뚜렷하였으며 등숙기간이 길수록 수량도 많았다. 4. 한기파종하여 수확시기를 빨리하면 미숙립의 발생이 많았고 늦게 수확하면 미이라병의 발생립율이 높았다. 5. 미이라병의 발생은 기온이 높은 춘천의 경우 항수량과 항수일수가 많은 정의 상관을 보였지만 기온이 낮은 진부에서는 유의성이 없었다. 6. 파종기에 관계없이 한기 수확하여 수분이 많은 종자는 건조과정에서 부패립이 많이 발생하였다. 7. 하대두 종자 생산을 위한 재배는 평야지인 춘천보다는 고랭지인 진부가 유리하였고 진부에서 5월15일부터 6월 5일 사이에 파종하여 개화후 45-50일에 수확하는 것이 발아율이 높고 종자 생산량도 많았다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.92-92
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• 2020

The phenolic contents in soybean seeds are affected by genetic and environmental factors. In the present study, 24 black soybeans and a control variety were grown in Korea and grouped as small (<13 g), medium (13 - 24.0 g), and large (>24 g) based on their seed weight. The total phenolic content (TPC) and antioxidant activities were analyzed and compared with their seed weight. The TPC was in the ranges 2.016 - 5.535, 1.992 - 4.679, and 1.829 - 4.621 mg GAE/g in small, medium, and large seeds with an average of 3.824, 3.108, and 3.626 mg GAE/g, respectively. The FRAP and DPPH activities were in the ranges 4.327 - 6.394 and 0.354 - 0.403 mg AAE/g in small seeds, 2.510 - 6.147 and 0.356 - 0.417 mg AAE/g in medium seeds, and 3.024 - 6.389 and 0.344 - 0.405 mg AAE/g in large seeds, respectively. Besides, ABTS activity was in the ranges 4.833 - 4.967, 3.636 - 4.871, and 4.554 - 4.956 mg TE/g in small, medium, and large seeds, respectively. Small seeds had the highest average FRAP (5.498 mg AAE/g) and ABTS (4.902 mg TE/g) activities while large seeds showed the highest average DPPH (0.387 mg AAE/g) activity although the variations were not significant (p > 0.05). TPC showed positive correlation with every antioxidant activity. However, the TPC correlation was significant (p < 0.05) only with DPPH (r = 0.673). Overall, small seeds presented a relatively high TPC and maximum antioxidant activities. Hence, black soybeans with small seeds could be important sources of high phenolic concentration and provide improved health benefits.

• 현장농수산연구지
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• 제26권1호
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• pp.16-21
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• 2024

본 연구는 해마다 수입을 많이 해오고 있는 콩과 녹비작물 종자의 발아율을 높이기 위해서 연구되었다. 열탕처리는 콩과 식물에서처럼 두꺼운 종피로 인해 휴면상태에 빠지는 종자의 발아율 향상 및 발아속도 증진을 위해 사용하는 방법이다. 클로버류와 자운영은 열탕처리가 무처리 보다 발아일수와 발아속도가 다소 높았다. 하지만 발아율과 발아세의 경우 무처리가 열탕처리보다 다소 높았지만 유의미한 차이를 보이지 않아 열탕처리가 발아율에 효과가 있다고 판단되지는 않는다. 네마장황의 경우 발아율과 발아속도를 높이는데 있어서는 염수선 처리가 가장 효과가 좋았고, 열탕처리는 초기 발아속도를 높일수는 있지만 발아율을 높이는데는 적합하지 않은 것으로 보였다. 헤어리베치의 경우 수입산 헤어리베치와 국내산 헤어리베치(토익과 청풍보라)에서 열탕처리와 염수선처리는 발아율에는 효과가 없었으나 발아세, 발아속도, 평균발아일수에는 다소 효과가 있다고 판단된다. 따라서 콩과 녹비작물과 같이 두꺼운 종피로 인하여 휴면상태에 빠지는 종자의 경우 발아율 향상과 발아속도를 향상시키기 위해 열탕처리나 염수선처리를 하는 것이 좋을것이라 사료된다.