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http://dx.doi.org/10.9721/KJFST.2012.44.5.600

Identification of Novel Bacillus subtilis IDCC 9204 Producing a High-Level Fibrinolytic Enzyme and Properties of NK-IL9204  

Lee, Seung-Hun (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
An, Gwangmin (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Kim, Heu-Hang (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Kang, Jae-Hoon (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Kang, Dae-Jung (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Publication Information
Korean Journal of Food Science and Technology / v.44, no.5, 2012 , pp. 600-606 More about this Journal
Abstract
A Bacillus sp. that produces fibrinolytic enzyme was isolated from Cheonggukjang, a traditional Korean soybean-fermented food. According to 16S rRNA gene base sequencing, the bacillus was identified as a variety of Bacillus subtilis, and named Bacillus subtilis IDCC 9204. Fibrinolytic enzyme NK-IL9204 was stable up to $60^{\circ}C$ and within pH range of 5-10. Purified NK-IL9204 was detected through fibrin zymography. The molecular weight and isoelectric point of the enzyme were estimated to be 27.7 kDa and 6.7 by SDS-PAGE and 2D electrophoresis, respectively. Its amino acid sequence was similar to that of nattokinase (identities 99.5%) and different from that of nattokinase BPN (identities 86.4%). The plasma fibrinolytic activity of NK-IL9204 was measured by euglobulin clot lysis times (ECLT). The NK-IL9204 was orally administered to SD rats for 3 weeks (1,000 FU/rat/day). The ECLT was significantly shortened by supplementation of NK-IL9204.
Keywords
Bacillus subtilis IDCC 9204; NK-IL9204; fibrinolytic enzyme;
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