• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.4
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• pp.368-372
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• 1999

The practicality of utilizing chitosan as a natural antimicrobial compound to reduce soybean sprout rot was tested. Domestic and imported soybean seeds were soaked for 6 hours in solutions containing different levels of chitosan and acetic acid (glacial), and cultured at $25^{\circ}C$ for 6 days. In case of domestic seeds, soaking with 1,000ppm chitosan increased germination percentage, hypocotyl thickness, total length, and fresh weight of sprouts by 4%, 5%, 2%, and 1%, respectively. The total sprout yield was increased by chitosan in a concentration-dependent manner in that 1,000ppm chitosan resulted in 8% increment of total yield (7.47kg sprouts/kg seed). Chitosan significantly reduced sprout rot percentage to 7.0% compared to control (13.8%), and consequently enhanced marketable sprout yield by 39%. Compared to domestic seeds, the imported soybean seeds exhibited very low germination percentage regardless of chitosan treatments. Chitosan, nevertheless, consistently induced yield increment and rot decrement in imported soybean sprouts. Although 100ppm acetic acid was effective in reducing sprout rot percentage down to 11.8%, its yield-increasing effects were not as prominent as chitosan. In conclusion, soaking soybean seeds with chitosan seems to be a practical method to enhance the efficiency of soybean sprout production.

• Korean Journal of Environmental Agriculture
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• v.20 no.1
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• pp.50-56
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• 2001

Thirty-three bacterial and fungal strains were isolated from the rotten soybeans and soybean sprouts to isolate pathogenic microorganisms which cause soybean sprouts rot during soybean sprouts cultivation. In pathogenicity tests of the isolates on soybean sprouts, two isolates(K-17 and K-28) caused soybean sprouts rot and were identified as Erwinia carotovora and Fusarium sp., respectively. To isolate antagonists aganist K-17 and K-28 pathogens, bacteria were isolated from various soybean-cultivated soils and screened by the inhibition zone method. A bacterial isolate(J-232) which inhibited growth of both pathogens was identified as Pseudomonas fluorescens and further examined. The culture filtrate of P. fluorescens J-232 (dilution rate of 500 times) inhibited the growth of Erwinia carotovora K-17 and Fusarium sp. K-28 both on potato dextrose agar medium and on soybean sprouts cultivated in vessel. The development of soybean sprouts rots was observed during cultivation by inoculation of soybean seeds with culture filtrate of both pathogens. The combined inoculation of soybean seeds with culture filtrate of antagonistic bacterium and that of pathogens prevented soybean sprouts rot, and the growth of soybean sprouts was similar to that of control. The soybean sprouts inoculated with antagonists culture filtrate alone did not develop soybean sprouts rot, and the growth of the seedlings was shown to be slightly promoted as compared with that of control.

• Korean Journal of Food Science and Technology
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• v.32 no.5
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• pp.1122-1127
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• 2000

Cultivation methods for clean soybean sprouts were investigated using irrigating solutions with grapefruit seed extract, chitosan and phosphate buffer. Chitosan and phosphate buffer did not inhibit the growth of soybean sprouts and increased the yield. Especially phosphate buffer was effective in yield increase and rot inhibition. As the times of irrigation with phosphate buffer increased, the yield of soybean sprouts increased up to 12.3% compared to that of the control. The high increase of yield and rot inhibition of soybean sprouts were detected even in $4{\sim}8$ times irrigation with phosphate buffer.

• Korean Journal Plant Pathology
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• v.13 no.1
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• pp.13-17
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• 1997

A causal agent of bacterial soft rot occurring in soybean sprout cultivation in Korea was isolated and identified, and its incidence in several sprout-soybean cultivars was examined. Infected soybean seeds became light brown and whitish, and could not germinate until 3 days after seeding, accompanying rotting of soybean seeds and sprouts. The causal organism isolated from the rotten seeds and sprouts was identified as Erwinia carotovora subsp. carotovora on the basis of its pathogenicity, morphological and physiological characteristics and the results of the Biolog GN microplate test program. The bacterial soft rot by E. c. subsp. carotovora was firstly described in soybean sprout in Korea, and we name it“the bacterial soft rot of soybean sprout”. The disease occurred more frequently in Nam-hae and Fu-reun sprout-soybean cultivars than in Eun-ha, So-baek, and Ik-san cultivars.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.584-589
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• 2000

In order to inhibit rot of soybean sprouts, presoaking treatments of soybeans with various solutions were studied. Optimal soaking time was 20min and citric acid, chitosan, GFSE(Grapefruit seed extract) were more effective. Most of presoaking treatments decreased the rot ratio of soybeans considerably, and didn't decrease germination ratio of them compared to control. Particularly GFSE, chitosan and phosphate buffer were effective. Presoaking treatments besides ethanol showed increased weight and yield of soybean sprouts compared to control, and particularly chitosan treatment increased yield of 67%, weight of 6.9% and length of 9.5% of soybean sprouts compared to control. Presoaking treatments decreased total microbial count of soybeans and rot of soybean sprouts during cultivation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.6
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• pp.994-998
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• 2002

The effect of heat-shock treatment of soybean on the quality characteristics of soybean sprouts was investigated. Six groups of soybeans were heat-treated in 35, 40, 45$^{\circ}C$ for 60 and 120 min respectively and then cultivated by a standard method to measure growth parameters, rot ratio, color, vitamin C content and sensory characteristics. Soybean sprouts of 4$0^{\circ}C$/120 min treatment showed the higher weight, while 4$0^{\circ}C$/60 min sample showed thicker hypocotyl and longer cotyledon than the control. In both rot ratio and viable cell count, the lowest values were obtained in soybean sprouts of control and also in 4$0^{\circ}C$/60 min treated samples, while other heat-treated samples showed higher rot ratio and cell counts than the control. Heat treatment of soybean increased the lightness and decreased the yellowness of cotyledon of sprouts. Vitamin C contents of sprouts were increased by heat treatment. Sensory test showed that the heat treatment reduced benny odor and bitter taste but enhanced the nutty taste. Soybean sprouts of 4$0^{\circ}C$/60 min resulted in significantly higher scores in overall appearance and palatability than other samples.

• Journal of agriculture & life science
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• v.50 no.1
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• pp.57-64
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• 2016

Soybean sprouts have been a considered a nutrient-rich vegetable for hundreds of years. To evaluate the seedlot quality of soybean sprouts grown, and to evaluate a method for reducing the presence of improper seeds in soybean seedlots, microbes associated with soybean sprout rot were isolated from samples collected. Morphological characteristics and gas chromatography profiles of the cultured fungal and bacterial strains were identified. Eight types of improper seeds were identified: purple stain(Ps), black rot(Br), seed coat black spot(Cb), wrinkled seed(Ws), brown hilum(Bh), seed coat fracture(Cf), unripe seed(Us), and brown seed coat(Bc). The improper seeds were also dipped into 15%, 20%, and 25% NaCl solutions, as well as a saturated solution of NaCl, for 1min. As the NaCl concentration increased, the number of floating improper seeds increased as well. The highest floating rates were observed for the Cf seeds.

• Preventive Nutrition and Food Science
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• v.8 no.4
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• pp.336-342
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• 2003

The effects of cultivation methods (cultivation of curled-shaped type, M-1; conventional cultivation, M-2; growing after treatment with a growth regulator, M-3; cultivation by the combination of M-1 and treatment with oak charcoal, M-4) on the quality characteristics of soybean sprouts were studied by the measurement of growth characteristics. This study also investigated the changes in shelf-life stability of the new soybean sprouts (NSB) using M-4, which was cultivated with oak charcoal and treated with antimicrobial Citrus sunki seed extract. Among the soybean sprouts grown for six days at the high temperature and humidity environment (90$\pm$5% RH, 25$\pm$1$^{\circ}C$), M-1 revealed no significant difference in terms of quality, such as the harvest yield, the rot rate and the growth characteristics when compared with M-2. M-3 showed no significant difference in growth characteristics, of hardness, and sensory evaluation scores when compared with the soybean sprouts grown by conventional methods. NSB had a low number of total microorganisms and had a better appearance after five days of storage than did the control group (M-2). These findings demonstrate that chemical-free and clean soybean sprouts can be grown by combining oak charcoal and antimicrobial Citrus sunki seed extract, thereby meeting the consumer demand for safe, chemical free sprouts.

• Journal of Bio-Environment Control
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• v.9 no.2
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• pp.101-106
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• 2000

The practicality of utilizing ventilation pipe to enhance soybean sprout productivity by lowering commodity temperature and resultant sprout rot was tested. The ventilation pipes with holes for aeration were vertically installed inside of a sprout cultivation container prior to adding soaked soybean seeds. The time-series changes in sprout commodity temperature and resultant growth, yield, marketable sprout percentage, and rot of soybean sprouts were measured. The ventilation pipes effectively decreased sprout commodity decreased sprout commodity temperature by 4.7$^{\circ}C$. Ventilation pipes also enhanced sprout yield and marketable sprout percentage by 3.9% and 4.0%, respectively, while reducing sprout rot by 4.1%. In conclusion, ventilation pipe seems a practical and effective non-chemical method to enhance the productivity of soybean sprouts.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2000.11a
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• pp.47-50
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• 2000