• 한국육종학회지
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• 제51권3호
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• pp.214-221
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• 2019

'아람'은 보석(IT213209)과 Camp (IT267356)를 모본으로 2007년 인공교배 하여 F₁, F₂ 분리집단을 전개하고 F₃-F₄ 세대는 세계 채소 센터(AVRDC, Asian Vegetable Research and Development Center)에서 세대촉진을 수행하고 F₅ 세대에서 계통을 선발하였다. 2012년~2013년 생산력검정시험을 통해 수량과 농업적 형질을 평가하고, 2014~2016년 수원, 나주, 달성, 제주에서 지역적응시험을 수행하여 지역별 적응성과 재배 안정성을 평가하였다. 아람은 유한신육형이며 엽형이 삼각형, 화색이 백색, 모용색이 회색, 협색은 황색이며 종실은 소립 구형으로 백립중이 9.9 g으로 풍산나물콩보다 1.0 g 가볍고 종피색은 황색, 배꼽색은 담갈색의 질적 특성을 가지고 있다. 개화기는 8월 5일, 성숙기는 10월 15일로 만숙종이며 풍산나물콩 보다 7일 느리다. 경장은 65 cm, 착협고는 13 cm, 마디수는 16개, 분지수는 4.5개이며 분지가 길고 각도가 작은 도원추형 초형이다. 내병성 검정결과 불마름병, 종자 이병립율, 검은뿌리 썩음병, 콩나방 저항성 등이 모두 강하였으며, 콩모자이크바이러스는 G5, G6H, G7H 접종 시 모자이크 증상이 나타났으나 3년간의 자연 이병 검정결과에서는 병징이 조사되지 않았다. 발아특성은 발아세와 발아율 모두 풍산나물콩보다 우수하였으며 콩나물 특성은 풍산나물콩과 비슷한 수준이었다. 수량성은 지역적응시험 결과 남부지역 평균 3.59 ton/ha로 풍산나물콩 대비 12% 증수되었다.

• Journal of Applied Biological Chemistry
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• 제50권2호
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• pp.63-69
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• 2007

Compositional components such as isoflavone, protein, oil, fatty acid, and free sugar in Korean vegetable soybeans were examined with four cultivars including Hwaeomputkong, Keunolkong, Mirang, and Danmi 2. In the isoflavone, Mirang cultivar showed the highest content ($967.1{\mu}g/g$), whereas Keunolkong was the lowest content ($535.9{\mu}g/g$). The malonylglucosides were the predominant isoflavone type followed by the glucoside, aglycone, and acetyl glucoside forms, respectively. In the protein content, Hwaeomputkong was the lowest (41.7%) and Danmi 2 was the highest (45.9%). The oil contents were 11.5 to 21.2% and Mirang cultivar was the lowest. The fatty acid compositions of the oil extracts exhibited that linoleic acid was the highest (33.6-42.5%), followed by oleic, palmitic, linolenic, and stearic acids. Whereas, oleic acid ($46.7{\pm}2.0%$) was more than linoleic acid ($33.6{\pm}1.3%$) in Mirang cultivar. In the free sugar contents, Hwaeomputkong cultivar showed the highest level and sucrose ($5.52{\pm}0.49%$) appeared to be most prevalent in vegetable soybeans.

• 농업과학연구
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• 제43권5호
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• pp.723-733
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• 2016

Soybeans [Glycine max (L.) Merill] are a rich source of antioxidants and other phytonutrients. Soybean sprouts contain many biologically active secondary metabolites and are rich in polyphenols, flavonoids, and phenolic compounds. In the present study, two soybean cultivars, Hosim, with high oleic acid (- 80% in total seed oil), and Pungsannamul, with normal oleic acid (- 23%) in seed, were examined for changes in the content of crude protein, crude oil, total flavonoids, total phenolics, and DPPH (1,1-diphenyl-2-picryl-hydrazyl) during the sprouting duration of 5 days. The protein content in both the varieties was found to increase by the days of sprouting. The crude oil content of Pungsannamul sprouts was found to be maximum on day 1 (16.9%, w/w) and decreased thereafter to reach to the level of 14.8% on day 5. No significant differences in the crude oil content of Hosim sprouts from day 1 to 5 were observed. Flavonoid content was found to increase up to day 4 and then dropped on day 5, in both the cultivars. Total polyphenol content showed a tendency to increase up to day 3 and started to decrease significantly from day 4. DPPH activity was found to increase up to day 5 in both the varieties. All the components studied in the high oleic acid soybean sprouts showed a change in content during the sprouting process similar to the change that would occur in normal oleic acid soybeans. The study showed that the contents of antioxidant, flavonoid, and polyphenol significantly increase during the sprouting.

• 동아시아식생활학회지
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• 제15권1호
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• pp.71-77
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• 2005

Proximate analysis, mineral and fatty acid composition of three conventional domestic soybean cultivars and two imported ones including glyphosate-tolerant HS2906 were evaluated by AOAC method, ICP-AES and gas chromatography. There were several differences in the proximate analysis among three conventional domestic soybean cultivars ; higher crude fat in the cultivar Hwanggumkong, higher crude protein in Pungsankong, and higher carbohydrate and crude ash in Duyukong. The ranges of contents of proximate components of domestic cultivars were similar to the data previously reported. There were no significant differences in proximate analysis between conventional soybean WS82 and glyphosate-tolerant HS2906 ; 23.55-23.90% of crude fat, 34.22-35.55% of crude protein, 6.25-6.45% of crude ash, and 25.35-26.47% of carbohydrate. The mineral and fatty acid compositions of HS2906 were similar to those of conventional soybeans previously reported.

• 한국작물학회지
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• 제49권5호
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• pp.419-422
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• 2004

Soybean is a major source of protein meal in the world. Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to identify RAPD markers linked to KTI protein allele using bulked segregant analysis. Cultivar Jinpumkong2 (TiTi) was crossed with C242 (titi, absence of KTI protein) and F. seeds were planted. The $F_1$. plants were grown in the greenhouse to produce $F_2$ seeds. Each $F_2$ seed from $F_1$. plants was analysed electrophoretically to determine the presence of the KTI protein band. The present and absent bulks contained twenty individuals each, which were selected on the basis of the KTI protein electrophoresis, respectively. Total 94 $F_2$ individuals were constructed and 1,000 Operon random primers were used to identify RAPD primers linked to the Ti locus. The presence of KTI protein is dominant to the lack of a KTI protein and Kunitz trypsin inhibit protein band is controlled by a single locus. Four RAPD primers (OPAC12, OPAR15, OPO12, and OPC08) were linked to the Ti locus. RAPD primer OPO12 was linked to Ti locus, controlling kunitz trypsin inhibitor protein at a distance of 16.0 cM. This results may assist in study of developing fine map including Ti locus in soybean.

• 한국작물학회지
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• 제35권2호
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• pp.171-175
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• 1990

1. Polyacrylamide gel electrophoresis를 이용하여 국내외 재래종과 야생종 대두 계통들의 trypsin inhibitor의 변이를 규명하기 위하여 본 시험이 시도되었으며 1706계통의 한국산 재래종과 103계통의 한국 야생종콩, 그리고 167계통의 외래 재배종과 71계통의 외국 야생종 대두가 공시되었다. 2. Trypsin inhibitor를 함유하지 않은 ti/ti형과 Ti c/c형은 한국 재래종에서만 발견되었으며, Ti$^{*}$ c형을 Hymowitz도 일본 대두품종에서 보고한 바 있으나 그도 이 계통은 한국 도래종일 가능성이 크다고 보고 한 바 있다. 3. 한국기원의 콩에서 trypsin inhibitor에 관한 이형접합형의 출현빈도가 외국 기원 콩 계통에서 보다 비교적 높았으며, 재래종에서 3.6%(N=61)와 야생종에서 9.7%(N=10)이었으며 종합적으로 보아 중국, 일본 등의 대두에 비해 한국 기원의 콩이 가장 큰 변이를 나타내고 있음을 확인하였다.

• 한국작물학회지
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• 제39권5호
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• pp.420-425
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• 1994

올콩의 수확시기에 따른 품질변화를 밝히고자, 대립종인 Yuhsuzumi와 소립종인 담양재내를 공시하여 수확시기별 종실의 수분함량, 입색, 발아율, 지방산 조성 등을 조사하여 다음과 같은 결과를 얻었다. 1. 협과 종실의 수분함량은 R7단계 전후까지는 협의 수분함량이 뚜렷이 높았으나, R8단계 이후에는 종실의 수분함량이 협보다 다소 높은 경향을 보였으며, 종실의 수분함량이 14%(종실 수분함량으로 본 수확적기) 내외가 되는 시점은 Yuhsuzumi에서는 생리적 성숙기(R7단계)로부터 24일, 담양재내는 8일이 소요되었다. 2. R8단계(고유 성숙협색기)에서 종실의 수분함량은 대립종인 Yuhsuzumi는 25.3%, 소립종인 담양재내는 14.5%를 나타내어 R8단계에 수확시 Yuhsuzumi는 철저한 건조과정이 필요하였다. 3. 수확기 지연에 의하여 Yuhsuzumi는 청색의 종실이 황변하고 입질이 저하되어 발아력이 낮아졌으나, 담양재내는 입질과 발아력의 차이가 없었다. 4. 수확기 지연에 의하여 Yuhsuzumi는 지방산 함량이 낮아졌고, 지방산 조성에 있어서는 Lino-lenic 산은 낮아지고 Oleic산은 증가되었다.

• 한국작물학회지
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• 제43권1호
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• pp.23-27
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• 1998

Inhibition of nodule formation and nitrogen fixation by soil nitrogen, primarily nitrate, is well known in legume plants. The present study was undertaken to evaluate the effect of ${NO_3}^-$ on the nodulation, nitrogenase activity, and growth of supernodulating soybean mutant and its wild type. A greenhouse study was conducted to compare two of supernodulating mutants, 'SS2-2' and 'nts 382', with the normal nodulating cultivar 'Sinpaldalkong 2' when grown in a 1-l styroform cup filled with sand, and fertilized with five levels of ${NO_3}^-$ (0, 2, 4, 8, and 12 mM). During the growth period, each plant was supplied two or three times a week with 50 mL of nutrient solution. Supernodulating soybean mutants, SS2-2 and nts 382, showed more nodules and nodule mass, and greater $C_2\;H_2$ activity than the wild type, Sinpaldalkong 2, regardless of the level of exogeneous nitrogen supply. On the other hand, total dry weight of SS2-2 mutant, which was smaller than Sinpaldalkong 2, did not respond to the various ${NO_3}^-$-N levels. This suggested that supernodulating SS2-2 mutant could maintain fairly high total dry weight at the low ${NO_3}^-$-N level, even in the absence of exogeneous ${NO_3}^-$-N in the nutrient solution. From the reduced top growth and high nitrogen fixing ability of supernodulating mutants, it was surmised that supernodulating mutant could potentially protect agricultural environments from pollution through the reduction in nitrogen fertilization as well as maintain fairly high yield with increasing planting density.

• 한국자원식물학회지
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• 제27권6호
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• pp.610-621
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• 2014

Phenolic compounds such as isoflavones contribute to the antioxidant properties of soybean. This study investigated differences in the phenolic content and antioxidant activity of ten soybean cultivars grown in two different locations in Korea. We observed a wide range of variation in total isoflavone and phenolic content by location and cultivar. The total isoflavone content of cultivars grown at Pyeongchang (sub-highland) was significantly higher than that in cultivars grown at Gangneung (lowland). In contrast, the total phenolic content of cultivars grown at Gangneung was greater than that of cultivars grown at Pyeongchang. The radical scavenging activity of DPPH was similar to that of the total phenolic content rather than that of the total isoflavone content. These results suggest that antioxidant activity of soybean was associated with phenolic compounds rather than isoflavones. To identify the individual antioxidant components, we used an on-line HPLC-$ABTS^+$-based assay system, ESI/MS, and NMR. The results showed that the strongest antioxidant activity was linked to epicatechin.

• Plant Resources
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• 제3권3호
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• pp.184-193
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• 2000

Soybean [Glycine max (L.) Merr.] seed weight is a important trait in cultivar development. Objective of this study was to identify and confirm quantitative trait loci (QTLs) for seed weight variation in the F2 and F2:3 generations. QTLs for seed weight were identified in F2 and F2:3 generations using interval mapping (MapMaker/QTL) and single-factor analysis of variance (ANOVA). In the F2 plant generation (i.e., F3 seed), three markers, OPL9a, OPM7a, and OPAC12 were significantly (P<0.01) associated with seed weight QTLs. In the F2:3 plant row generation (i.e., F4 seed), five markers, OPA9a, OPG19, OPL9b, OPP11, and Sat_085 were significantly (P<0.01) associated with seed weight QTLs. Two markers, OPL9a and OPL9b were significantly (P<0.05) associated with seed weight QTLs in both generations. Two QTLs on USDA soybean linkage group C1 and R were identified in both F2 and F2:3 generations using interval mapping. The linkage group C1 QTL explained 16% of the variation in seed weight in both generations, and the linkage group R QTL explained 39% and 41% of the variation for F2 and F2:3 generation, respectively. The linkage group C2 QTL identified in F2:3 generation explained 14.9% of variation. Linkage groups C1, C2 and R had previously been identified as harbouring seed size QTLs. The consistency of QTLs across generations and populations indicates that marker-assisted selection is possible in a soybean breeding program.