• Journal of Plant Biology
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• 제25권4호
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• pp.181-188
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• 1982

Chloroplasts isolated from tobacco (Nicotiana tabacum L. cv. Virginia 115) leaves have been transferred into protoplasts of soybean (Glycine max Merr. cv. Jangyeop) suspension-cultured cells with the help of polyethylene glycol (PEG). The increased yield in protoplasts of chloroplast uptake was depended upon the concentration of both PEG 4,000 and PEG 6,000. The highest yield(36%) occurred at 50% of both PEG, and the yield was decreased above this concentration. The rate of uptake with the incubation time was highest at one hour, then decreased. The process of the chloroplast uptake into the protoplasts was similar with that of a protoplast fusion, except forming invagination during uptake.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.145-145
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• 2017

Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The objective of this study was to identify the putative genes to foxglove aphid resistance in wild soybean, PI 366121 (Glycine soja Sieb. and Zucc.). One hundred and forty-one F4:8 recombinant inbred lines developed from a cross between susceptible variety, Williams 82 and foxglove aphid resistance wild soybean, PI 366121 were used. The two type of resistance response, antibiosis and antixenosis resistance were evaluated through choice and no-choice test, graded by the degree of total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 29,898 single-nucleotide polymorphism markers utilizing a Axiom(R) 180K soyaSNP array. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 was identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. The newly identified major QTL was consistent with previously reported QTL, Raso2, which showed around 5 times narrow down interval range with 8 candidate genes. Furthermore, total 1,115 soybean varieties including Glycine soja and Glycine max were exposed to germplasm screening, and 31 varieties, which showed significant antibiosis type foxglove aphid resistance were identified. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars and developing novel insecticides.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.106-106
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• 2017

The soybean [Glycine max (L.) Merr.] is one of the most important crop resources worldwide as food and forage. It is also important and valuable that to hold crop resources to have high genetic diversities. Recently, a core collection has been constructed in many plants to preserve the genetic resources of various plants. A core collection is small population to represent the genetic diversity of the total collection, and is of strategic importance as they allow the use of a small part of a germplasm collection that is representative of the total collection. Here, we developed the core collection consisting of 816 accessions by using approximately 180,000 (180K) single nucleotide polymorphisms (SNPs) developed in previous study. In addition, we performed genetic diversity and population structure analysis to construct the core collection from entire 4,392 collections. there were excluded sample call rates less than 93% and duplicated samples more than 99.9% according to genotype analysis using 180K SNPs from entire collections. Furthermore, we were also excluded natural hybrid resources which Glycine max and Glycine soja are mixed in half through population structure analysis. As a result, we are constructed the core collection of genetic diversity that reflects 99% of the entire collections, including 430 cultivated soybeans (Glycine max) and 386 wild soybeans (Glycine soja). The core collection developed in this study should be to provide useful materials for both soybean breeding programs and genome-wide association studies.

• 한국환경농학회지
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• 제7권1호
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• pp.52-57
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• 1988

acifluorfen이 대두(大豆)와 바랭이의 경엽(莖葉)에서 peroxidase의 활성(活性)에 어떻게 영향(影響)을 미치는가를 구명(究明)하여 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. Peroxidase 활성(活性)은 처리농도(處理濃度)가 높을수록 높았으며 처리시기(處理時期)가 늦을수록 낮았다. 2. 1.5부엽기(復葉期)에 처리(處理)했을 때는 신생엽(新生葉)인 2부엽(復葉)의 활성(活性)이 높았으나, 2.5부엽기(復葉期)에 처리(處理)했을 경우는 신생엽(新生葉)인 3부엽(復葉)의 활성(活性)이 오히려 낮았다. 3. 1.5부엽 (復葉) 처리(處理)의 신생엽(新生葉)인 2부엽(復葉)에서의 peroxidase 활성(活性)이 2.5부엽(復葉)의 처리(處理)의 2부엽(復葉)보다 높았다. 4. 모든 처리엽(處理葉)이 necrosis와 leaf burning현상이 나타났지만, 처리(處理) 14日(일) 후(後)에는 거의 정상으로 회부(回復)되었다. 5. 바랭이는 처리(處理) $1{\sim}2$일후(日後) peroxidase 활성(活性)이 현저히 증가하였고, 5 일후(日後)는 급격히 감소하였다. 이는 바랭이가 acifluorfen 처리후(處理後) $3{\sim}7$ 일내(日內)에 모두 고사(枯死)하였기 때문이었다.

• 분석과학
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• 제12권6호
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• pp.534-539
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• 1999

Penicillium, Aspergillus 및 Fusarium에 오염된 밀(Triticum aestivum L.), 콩(Glycine max Merr), 옥수수(Zea mays L.)에서 HPLC를 이용하여 주요 독소들을 검정하였다. Penicillium 독소인 brefeldin A는 밀에서 3.1~270 ppm, 콩에서 45~230 ppm, 옥수수에서 1030~1240 ppm 다량 검출되었다. Citreoviridin과 griseofulvin은 밀, 콩, 옥수수에서 각각 40~80 ppm과 3.6~26.0 ppm이 검출되었다. 그리고 citrinin과 patulin은 밀, 콩, 옥수수에서 각각 0.3~4.0 ppm과 420~3800 ppm로 검출되었고, 특히 옥수수에서 다량 검출되었다. Aspergillus 독소로 ochratoxin A는 밀에서 730 ppm, 콩에서 12.4 ppm, 옥수수는 310 ppm이 검출되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.16-16
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• 2020

• Plant Resources
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• 제5권1호
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• pp.1-6
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• 2002

Genetic improvement of the cultivated soybean [Glycine max (L.) Merr] may be possible through hybridization with its wild progenitor, G. soja Sieb. & Zucc. Interspecific cross between G. max (Hwangkeumkong) and G. soja (IT.182932) was made in the summer of 1997. In F$_2$ the percentage of plant height, nodes per plant, and pods per plant were high but gradually reduced from F$_2$ to F$_4$. In contrast pod length, seeds per pod, and 100-seeds weight were increased gradually through generations advanced. Wild variation as evident in F$_2$ in plant height, number of branches, pods per plant, and 100-seeds weight. Twenty six percent of the F$_2$, 44 % of the F$_3$ and 60% of the F$_4$ segregants showed more G. max traits. The combination of useful traits from both species is possible through interspecific hybridization. The characters that could be transferred from wild species to cultivated species are more pod number, better capacity, and resistance to disease and insects. The interspecific derivatives offer scope for selection for high grain yield. Therefore, introducing genes from G. soja to G. max could be contribute to greater genetic diversity of future cultivars. And semicultivated soybean had some desired characteristics including tolerance to adverse environments and multi-seed characters. It means the infusing of semicultivated germplasm to the cultivated soybean could increase number of seeds and pods per plant significantly, and consequently could enhance selecting potential on yield.

• Journal of Applied Biological Chemistry
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• 제59권3호
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• pp.261-264
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• 2016

In soybean (Glycine max (L.) Merr.) seeds, phosphorus (P) is primarily stored in the form of phytate, which is generally indigestible by monogastric animals such as human, pig, poultry, and fish. Thus, this study was conducted to isolate soybean mutants with high available P. Inorganic P content was assessed in a total of 1,266 ethyl methanesulfonate (EMS) $M_4$ lines from the Pungsannamul cultivar. Among the tested lines, four EMS lines (PE379, PE432, PE2205, and PE2503) showed higher mean inorganic P ($1.21-1.56gkg^{-1}$) than did the Pungsannamul cultivar ($0.90gkg^{-1}$). Additionally, six EMS lines (PE718, PE828, PE1466, PE1552, PE3378, and PE3386) showed lower mean inorganic P ($0.38-0.60gkg^{-1}$). The high inorganic P mutants isolated in this study will be further investigated for phytate and total P levels. Moreover, the high and low inorganic P lines will be utilized in a future study of the biochemical pathway of phytate.

• 한국육종학회지
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• 제41권4호
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• pp.603-606
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• 2009

Lipoxygenase and Kunitz trypsin inhibitor protein of mature soybean [Glycine max (L.) Merr.] seed are main anti-nutritional factors in soybean seed. A new soybean cultivar, "Gaechuck#1" with the traits of black seed coat, green cotyledon, lipoxygenase2,3 and Kunitz trypsin inhibitor protein free was developed. It was selected from the population derived the cross of "Gyeongsang#1" and C242. Plants of "Gaechuck#1" have a determinate growth habit with purple flowers, brown pubescence, black seed coat, black hilum, oval leaflet shape and brown pods at maturity. Seed protein and oil content on dry weight basis have averaged 39.1% and 16.2%, respectively. It has shown resistant reaction to soybean necrosis, soybean mosaic virus, Cercospora leaf spot and blight, black root rot, pod and stem blight, and soybean pod borer. "Gaechuck#1" matured on 5-10 October with a plant height of 50 cm. The 100-seed weight of "Gaechuck#1" was 23.2g. Yield of "Gaechuck#1" was averaged 2.2 ton/ha from 2005 to 2007.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.166-167
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• 2006