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Arthrospira platensis Mutants Containing High Lipid Content by Electron Beam Irradiation and Analysis of Its Fatty Acid Composition (전자빔 조사에 의해 지질 함량이 증대된 Arthrospira platensis 변이주 분리 및 지방산 분석)

  • Choi, Soo-Jeong;Kim, Young-Hwa;Kim, Andre;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
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    • v.24 no.6
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    • pp.628-632
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    • 2013
  • Arthrospira platensis (A. platensis) is an economically important microalgae because it has carbohydrates, lipids, proteins and a number of phytochemicals. It is also a valuable source used in the production of biodiesel and functional foods. In this study, A. platensis was exposed to electron beam irradation (240 kGy) and induced random mutagenesis for strain improvement. Several mutants were obtained, and the resulting mutant was designated as EB29. The growth rate and chlorophyll content of EB29 was similar to those of wild type. However, the lipid content of EB29 was increased seven-fold compared to that of wild type when comparing the nile red fluorescent intensity. Semi-quantitative analysis of EB29 using the calibration plot of standard lipid, triolein, represented $78.6{\mu}g/mL$, which increased 2 times compared to wild type ($41.4{\mu}g/mL$). When analyzing the fatty acid profile of EB29, polyunsaturated fatty acids (PUFAs), such as gamma-linolenic acid (GLA) in EB29 increased about six-fold. Moreover, fatty acids affecting the quality of biodiesel increased compared to that of wild type. Thus, electron beam could be used for the strain improvement of microalgae in order to accumulate PUFAs and alteration of fatty acid profile for biodiesel.

Evaluation of barley to replace milk by-product in weaning pig's diet

  • Jin, Kyung Young;Hong, Jin Su;Sin, Dong Wook;Kang, Hyo Kon;Jo, Yun Young;Lee, Geon Il;Jin, Xing Hao;Jang, Jae Cheol;Jeong, Jae Hark;Kim, Yoo Yong
    • Journal of Animal Science and Technology
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    • v.61 no.2
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    • pp.77-86
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    • 2019
  • The supplementation level of barley was limited because of high contents of fiber in monogastric animals. Barley contained high soluble fiber, thus it could prevent to diarrhea of weaning pigs. Moreover, as the barley break down by enzymes, free sugars come out from the barley, which could be used as an energy source in weaning pigs and replace milk by-products in weaning pig's diet. Therefore, present study was conducted to investigate the influence of barley to replace milk by-product in weaning pig's diet on growth performance, blood profile, nutrient digestibility, diarrhea incidence, and economic analysis in weaning pigs. A total of 112 crossbred ($[Yorkshire{\times}Landrace]{\times}Duroc$, weaned at 28 days of age) piglets were allotted to 4 treatments in a randomized complete block (RCB) design. Each treatment has 7 replications with 4 pigs per pen. Pigs were fed each treatment diet which containing different levels of barley (0%, 10%, 20%, and 30%) at the expense of whey powder and lactose. Three phase feeding programs were used for 6 weeks of growth trial (phase 1: 0-2 weeks; phase 2: 3-4 weeks; phase 3: 5-6 weeks). During 0-2 week, body weight (BW), average daily gain (ADG) and G:F ratio were decreased as barley level increased in the diet (linear response, p < 0.01). In blood profile, blood urea nitrogen was decreased as the barley level increased in the diet (linear, p < 0.01). However, no significant differences were observed in blood glucose level. In nutrient digestibility, crude fat digestibility was linearly increased as barley increased (linear, p < 0.01). The incidence of diarrhea was improved as increasing barley contents in all phases (linear, p < 0.01). These results demonstrated that supplementation of barley to replace milk by-product influenced negatively on growth performance during 0-2 week. However, the incidence of diarrhea and later growth performance from 3 week postweaning were improved as dietary barley level increased.

A Simple Design of an Imaging System for Accurate Spatial Mapping of Blood Oxygen Saturation Using a Single Element of Multi-wavelength LED (혈중 산소 포화도의 정확한 공간 매핑을 위한 다중 파장 LED 단일소자를 활용한 이미징 시스템 설계)

  • Jun Hwan Kim;Gi Yeon Yu;Ye Eun Song;Chan Yeong Yu;Yun Chae Jang;Riaz Muhammad;Kay Thwe Htun;Ahmed Ali;Seung Ho Choi
    • Journal of Biomedical Engineering Research
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    • v.44 no.6
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    • pp.450-464
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    • 2023
  • Pulse oximetry, a non-invasive technique for evaluating blood oxygen saturation, conventionally depends on isolated measurements, rendering it vulnerable to factors like illumination profile, spatial blood flow fluctuations, and skin pigmentation. Previous efforts to address these issues through imaging systems often employed red and near-infrared illuminations with distinct profiles, leading to inconsistent ratios of transmitted light and the potential for errors in calculating spatial oxygen saturation distributions. While an integrating sphere was recently utilized as an illumination source to achieve uniform red and near-infrared illumination profiles on the sample surface, its bulkiness presented practical challenges. In this work, we have enhanced the pulse oximetry imaging system by transitioning illumination from an integrating sphere to a multi-wavelength LED configuration. This adjustment ensures simultaneous emission of red and near-infrared light from the same position, creating a homogeneous illumination profile on the sample surface. This approach guarantees consistent patterns of red and near-infrared illuminations that are spatially uniform. The sustained ratio between transmitted red and near-infrared light across space enables precise calculation of the spatial distribution of oxygen saturation, making our pulse oximetry imaging system more compact and portable without compromising accuracy. Our work significantly contributes to obtaining spatial information on blood oxygen saturation, providing valuable insights into tissue oxygenation in peripheral regions.

Effects of Dietary Copper Source and Level on Performance, Carcass Characteristics and Lipid Metabolism in Lambs

  • Cheng, Jianbo;Fan, Caiyun;Zhang, Wei;Zhu, Xiaoping;Yan, Xiaogang;Wang, Runlian;Jia, Zhihai
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.5
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    • pp.685-691
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    • 2008
  • An experiment was conducted to determine the effect of dietary copper (Cu) on performance, carcass characteristics and lipid metabolism in lambs. Fifty DorperMongolia wether lambs (approximately 3 month of age; 23.80.6 kg of body weight) were housed in individual pens and were assigned randomly to one of five treatments. Treatments consisted of 1) control (no supplemental Cu), 2) 10 mg Cu/kg DM from Cu-lysine, 3) 20 mg Cu/kg DM from Cu-lysine, 4) 10 mg Cu/kg DM from tribasic copper chloride (Cu2(OH)3Cl; TBCC), 5) 20 mg Cu/kg DM from tribasic copper chloride. The Cu concentration was 6.74 mg/kg DM in the basal diet. Body weight was measured on two consecutive days at the start and the end of the 60-day experimental period. Blood samples were collected and then the lambs were slaughtered on d 60. Performance was not affected (p>0.05) by dietary Cu treatment. Cu-supplemented and control lambs had similar hot carcass weight, dressing percentage and longissimus muscle area, but Cu supplementation, regardless of source and level, reduced (p<0.01) 12th rib backfat and kidney fat in lambs. Plasma tumor necrosis factor-alpha (TNF-) and serum triglyceride concentrations were increased (p<0.05), total cholesterol concentrations were decreased (p<0.05) and nonesterified fatty acids (NEFA) concentrations tended to be increased (p<0.07) by Cu supplementation. However, Serum concentrations of HDL-cholesterol and LDL-cholesterol were not affected (p>0.05) by dietary treatment. Fatty acid profile of longissimus muscle was similar across treatments. These results indicate that Cu-lysine and TBCC are of similar availability in lambs. Cu supplementation given to DorperMongolia wether lambs altered lipid metabolism. The reduction in backfat depth may be due to copper altering TNF- metabolism in lambs. Supplementation of 10 or 20 mg Cu/kg DM showed similar effects on lipid metabolism in lambs.

A Study on the Horizontal and Vertical Distribution of Heavy Metal Elements in Slime Dump from Dukum Mines, Korea (덕음광산 선광광미와 주변토양의 중금속에 대한 수평.수직적인 분산에 관한 연구)

  • 박영석
    • Economic and Environmental Geology
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    • v.33 no.2
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    • pp.91-100
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    • 2000
  • It has been more than ten years since Dukun mine was abandoned. Tailings of waste deposits and slime dumps in the abandoned Dukum mine have been left to be deserted for fifty years. The results of fifty years of neglecting are nothing short of major environmental problems. Slime dumps have been exposed to air and water in the mine over ten years and then soil profile has been formed well. Soil in the upper layer (A horizon) is the light gray color due to the leaching of cations. Soil in the lower layer (A2 horizon, 0.2∼0.3m)is tinted with reddish brown and yellowish brown color due to the development of iron oxides and iron hydroxides. Soil in the lower part of B horizon of (1.0∼3.0m) with the growth of copper and zinc oxides exposes to the bluish green, light blue, and dark gray. Ranging from 3m to 8m in depth, 85 samples were taken from 22 sampling sites with 50m intervals located on the slime dump area with hand auger and trench (open cut). As tailings was distributed, heavy metal elements extracted by the process of surface water and ground water move and disperse in to the hydrosphere. Waste dumps were distributed in and around the mine and water draining from those dumps be a potential source of contamination. Soils, thus, can be dispersed into downslope and downstream through wind and water by clastic movement. These materials may be deposited in another horizon if the water is withdrawn, or if the materials are precipitated as a result of differences in pH, or other conditions in deeper horizons. These were primarily associated with acid mine drainage. The characteristics and rate of release of acid mine drainage are influenced by various chemical and biological reactions at the source of acid generations. Prolonged extration of heavy metal elements has a detrimental effect on the agricultural land and residental area. Twenty soil samples were collected from the agricultural land in the area (0∼30 cm). Seventeen samples were also taken from the sediment in the stream running alongside the dumps. The dispersion patterns of heavy metal elements are as follows: The content of As ranged 2∼6 ppm in a horizon, 20∼125 ppm in B horizon with large amount of clay mineral is concentrated and the content of Cd ranged 1∼2 ppm in A horizon, 4∼22 ppm in B horizon. Like Cd, the content of As, Cu, Zn, Pb in B horizon is higher than that in A horizon (approximately 5∼100 times). When soil formation proceeds in stages, it is necessary to investicate the B horizon with the concentration of heavy metal and preventive measures will have to established.

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High Loading for Air Pollution in the Byunsan Peninsula of Korea by an Interplay of the Saemangeum Project and Winter Monsoon

  • Ma, Chang-Jin;Kang, Gong-Unn;Kim, Ki-Hyun
    • Asian Journal of Atmospheric Environment
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    • v.6 no.3
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    • pp.234-243
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    • 2012
  • The wintertime high loading for atmospheric pollutants is certainly expected in the Byunsan Peninsula of Korea because of a great-scale reclamation project having construction of 33 km tidal sea dike impounding an area of over 40,000 ha and long-range transport. The goal of this study is to trace the origin of this wintertime burden for ambient particulate matter (hereafter called "PM") in the Byunsan Peninsula of Korea. The size-segregated (i.e., cutoff size from 0.01 ${\mu}m$ to 4.7 ${\mu}m$) PM sampling was conducted at a ground-based site of Byunsan Peninsula located in the west coast of Korean Peninsula during the height of dike constructing. Data archived in this study are the mass concentrations of ionic, elemental, and carbonic components in size-fractioned PM. The elemental mass of individual submicrometer particles was also analyzed. The sum of 5-source (i.e., elemental carbon, organic materials, inorganic secondary pollutants, crustal matter, and sea-salts) concentrations shows the bimodal distribution (major and minor peaks formed around $D_p$, 0.65 ${\mu}m$ and $D_p$, 4.7 ${\mu}m$, respectively) by border with 0.19 ${\mu}m$ of cutoff size. The concentrations of EC in $PM_{1.1-0.01}$ in winter and spring times were 4.62 ${\mu}g\;m^{-3}$ and 3.74 ${\mu}g\;m^{-3}$, respectively. Elemental masses of submicron individual particles are classified into two groups, i.e., the major elements (Cl, Al, Si, S, and P) and the minor trace elements. Cluster analysis differentiated the elements in submicron individual particles into 4-cluster. Among them, three clusters are in agreement with the major (Al, Si, S, and P), minor (Fe, Ca, and K), and trace compositions of coal burning. Meanwhile, Cl classified as an independent cluster has different source profile which was mainly due to the Saemangeum seawall project. Some highly toxic elements (e.g., Cr, Mn, and As (and/or Pb)) were also detected in some part of submicron individual PM. As a consequence, the combination of the Saemangeum project and winter monsoon played a considerable part in the double aggravation of wintertime air pollution in the Byunsan Peninsular.

Evaluation of Quality of Life in Patient with Temporomandibular Disorders (측두하악관절장애를 가진 환자에서의 삶의 질의 평가)

  • Jung, Jin-Suk;Hur, Yun-Kyung;Choi, Jae-Kap
    • Journal of Oral Medicine and Pain
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    • v.31 no.2
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    • pp.127-139
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    • 2006
  • Objectives: To explore the quality of life in patients with temporomandibular disorders and to evaluate it in terms of source and duration of the pain. Methods: A total of 61 patients with temporomandibular disorders participated in this study. According to pain source, they were divided into 2 groups, masticatory muscle pain (MMP) group and intracapsular pain (ICP) group. And each group was divided into acute phase group (pain duration <6 months) and chronic phase group (pain duration=6 months). The Medical Outcomes Study 36-Item Short-Form Health Survey (SF-36) was used to measure patients' quality of life. The scores for eight-scale profile and the physical component summary (PCS) and mental component summary (MCS) of the SF-36 were compared between groups (MMP vs. ICP and acute vs. chronic). Student t-test was used to analyze the difference of the scores of the SF-36 between MMP and ICP groups. Results: MMP group showed significantly lower score in the 3 scales of the SF-36 (Role limitations due to emotional problems, Vitality, Bodily pain) when compared to ICP group. In acute phase there was no significant difference between MMP and ICP group in PCS as well as MCS scores, but in chronic phase MMP group showed significantly lower MCS score than ICP group. Conclusions: The masticatory muscle pain in patients with temporomandibular disorders,negatively influences the quality of life especially in chronic phase, and the mental components of quality of life are significantly interfered in the TMD patients with chronic masticatory muscle pain.

Enhancement of Ionospheric Correction Method Based on Multiple Aperture Interferometry (멀티간섭기법에 기반한 이온왜곡 보정기법의 보완)

  • Lee, Won-Jin;Jung, Hyung-Sup;Chae, Sung-Ho;Baek, Wonkyung
    • Korean Journal of Remote Sensing
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    • v.31 no.2
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    • pp.101-110
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    • 2015
  • Synthetic Aperture Radar Interferometry (InSAR) is affected by various noise source such as atmospheric artifact, orbital error, processing noise etc.. Especially, one of the dominant noise source for long-wave SAR system, such as ALOS PALSAR (L-band SAR satellite) is the ionosphere effect because phase delays on radar pulse through the ionosphere are proportional to the radar wavelength. To avoid misinterpret of phase signal in the interferogram, it is necessary to detect and correct ionospheric errors. Recently, a MAI (Multipler Aperture SAR Interferometry) based ionospheric correction method has been proposed and considered one of the effective method to reduce phase errors by ionospheric effect. In this paper, we introduce the MAI-based method for ionospheric correction. Moreover we propose an efficient method that apply the method over non-coherent area using directional filter. Finally, we apply the proposed method to the ALOS PALSAR pairs, which include the west sea coast region in Korea. A polynomial fitting method, which is frequently adopted in InSAR processing, has been applied for the mitigation of phase distortion by the orbital error. However, the interferogram still has low frequency of Sin pattern along the azimuth direction. In contrast, after we applied the proposed method for ionospheric correction, the low frequency pattern is mitigated and the profile results has stable phase variation values within ${\pm}1rad$. Our results show that this method provides a promising way to correct orbital and ionospheric artifact and would be important technique to improve the accuracy and the availability for L-band or P-band systems.

Review of Nitrous Oxide Emission by Denitrification in Subsurface Soil Environment (심층토에 있어서 탈질화에 의한 $N_2 O$ 방출의 평가)

  • Chung Doug-Young;Jin Hyun-O;Lee Chaang-Hwan
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.1 no.2
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    • pp.160-164
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    • 1999
  • Subsurface environments, including the intermediate vadose zone and aquifers, may be contributing to increased atmospheric concentrations of $N_2$O. Denitrification appears to be the major source of $N_2$O in the subsurface environment. In the intermediate vadose zone, the level of denitrifying activity is dependent on the soil morphology, particularly stratified layers within the soil profile, which impede water and solute movement and create conditions favorable for denitrification. Movement of organic C from the soil surface appears to support denitrifying activity by providing an energy source and increasing the consumption of $O_2$. Denitrirication and $N_2$O production have been observed in aquifers but appear to be of greatest significance in shallow unconfined aquifers. The lack of organic C, N $O_2$, or anaerobiosis is often a limiting factor for activity but seems to be site specific. The presence of denitrifying bacteria does not appear to be a major limitation, based on published results, but the ubiquity of denitrifiers in subsurface environments needs to be confirmed. The fate of the $N_2$O produced in subsurface environments is unknown. Transport of $N_2$O by up ward diffusion, by outgassing at contacts with surface waters, and by ground water use need to be quantified to determine the contribution to atmospheric $N_2$O. Contamination of subsurface environment with N $O_3$$^{ }$ and organics has the potential for increasing the contribution to atmospheric $N_2$O by enhancing denitrification .

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Dietary Iron Intake of Koreans Estimated using 2 Different Sources of Iron Contents are Comparable: Food & Nutrient Database and Iron Contents of Cooked Foods in the Korean Total Diet Study (우리 국민의 철 섭취량 평가: 식품별 영양성분 함량자료와 한국형 총식이조사 기반 추정량 비교)

  • Lee, Jeeyeon;Kwon, Sung Ok;Yeoh, Yoonjae;Seo, Min Jeong;Lee, Gae Ho;Kim, Cho-il
    • Korean Journal of Community Nutrition
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    • v.27 no.3
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    • pp.245-253
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    • 2022
  • Objectives: This study was conducted to find out if the dietary iron intake of Koreans estimated by 2 different methods (iron content sources) using the food intake data from the Korea National Health and Nutrition Examination Survey (KNHANES) are comparable. One method was based on the KNHANES's Food & Nutrient Database (FND) derived mainly from the Korean Food Composition Table and the other used the iron content (IC) of food samples processed in the Korean Total Diet Study (KTDS). Methods: Dietary intake data from the 2013-2016 KNHANES was used to select representative foods (RFs) in KTDS for iron analysis. Selection of the RFs and cooking methods for each RF (RF × cooking method pair) was performed according to the 'Guidebook for Korean Total Diet Studies' and resulted in a total of 132 RFs and 224 'RF × cooking method' pairs. RFs were collected in 9 metropolitan cities nationwide once or twice (for those with seasonality) in 2018 and made into 6 composites each, based on the origin and season prior to cooking. Then, the RF composites prepared to a 'table ready' state for KTDS were analyzed by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Dietary iron intake of the Korean population was estimated using only RFs' intake data based on the 2 sources of iron content, namely FND-KNHANES and IC-KTDS. Results: RFs in KTDS covered 92.0% of total food intake of Koreans in the 2016-2018 KNHANES. Mean iron intake of Koreans was 7.77 mg/person/day by IC-KTDS vs 9.73 mg/person/day by FND-KNHANES. The major food groups contributing to iron intake were meats (21.7%), vegetables (20.5%), and grains & cereals (13.4%) as per IC-KTDS. On the other hand, the latter source (FND-KNHANES) resulted in a very different profile: grains & cereals (31.1%), vegetables (16.8%), and meats (15.3%). While the top iron source was beef, accounting for 8.6% in the former, it was polished rice (19.2%) in the latter. There was a 10-fold difference in the iron content of polished rice between 2 sources that iron intakes excluding the contribution by polished rice resulted in very similar values: 7.58 mg/person/day by IC-KTDS and 7.86 mg/person/day by FND-KNHANES. Conclusions: This study revealed that the dietary iron intake estimated by 2 different methods were quite comparable, excluding one RF, namely polished rice. KTDS was thus proven to be a useful tool in estimating a 'closer-to-real' dietary intake of nutrients for Koreans and further research on various nutrients is warranted.