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Amperometric Enzyme Electrode for the Determination of $NH_4^+$ ($NH_4^+$ 정량을 위한 Amperometric Enzyme Electrode)

  • Moo Lyong Seo;Jae Sang Kim;Shim Sung Lee;Zun Ung Bae;Heung Lark Lee;Tae Myung Park
    • Journal of the Korean Chemical Society
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    • v.37 no.11
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    • pp.937-942
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    • 1993
  • Enzyme electrodes for amperometric measurement of ammonia was prepared by immobilization of L-glutamate dehydrogenase on an Immobilon-AV Affinity membrane and attachment to a glassy carbon electrode. Reduced nicotinamide adenine dinucleotide (NADH) was used as the electroactive species. The electrochemical oxidation of NADH was monitored at +1.0 volt vs. Ag/AgCl. Response was linear from $4.0\;{\times}\;10^{-5}\;to\;4.0\;{\times}\;10^{-4}$ M. The detection limit was 2.0 ${\times}\;10^{-6}$ M. Response time, the optimum pH and life time of enzyme immobilized membrane were 2 min, pH 7.3∼7.6 (Dulbecco's buffer solution) and about 25 days respectively. When the enzyme electrode was applied to the $NH_4^+$ determination with amperometric method, other physiological materials had no interference.

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Anti-oxidative Activity of Ethyl acetate Fraction of the Dried Ginger in Caenorhabditis elegans (건강 에틸아세테이트 분획의 예쁜꼬마선충 내의 항산화 효과)

  • Lee, Eun Byeol;Kim, Jun Hyeong;Leem, Jae-Yoon;Kim, Hye-Soo;Kim, Dae-Sung;Eun, Jae Soon;Han, Sooncheon;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.47 no.2
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    • pp.179-185
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    • 2016
  • Ginger (Zingiber officnale Roscoe, Zingiberaceae), which is a well-known food seasoning, has been used as a traditional medicine for the treatment of gastrointestinal disorder, vomiting and cough in Korea, China and Japan. Ethanol extract from the dried ginger (DG) was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions the ethyl acetate soluble fraction (EDG) showed the most potent DPPH radical scavenging and superoxide quenching activities. To know the effect of antioxidant activities of EDG, we tested the activities of superoxide dismutase (SOD) and catalase together with oxidative stress tolerance and intracellular ROS level in Caenorhabditis elegans. To investigate whether EDG-mediated increased stress tolerance was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain including CF1553. Consequently, EDG elevated SOD and catalase activities of C. elegans, reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, EDG-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

Electrochemical Behaviors and Analytical Application of Copper-1,5,9,13-Tetrathiacyclohexadecane Complex in Acetonitrile (아세토니트릴 용매 중에서 Copper-1,5,9,13-Tetrathiacyclohexadecane착물의 전기화학적 거동과 그 분석적 응용)

  • Moo-Lyong Seo;Bu-Yong Lee;Myung-Ja Choi;Bae Jun Ung;Park Tae Myeong
    • Journal of the Korean Chemical Society
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    • v.36 no.3
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    • pp.412-418
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    • 1992
  • The electrochemical behaviors and analytical application of copper-1,5,9,13-tetrathiacyclohexadecane[16-ane-$S_4$] complex in acetonitrile(AN) solution have been investigated by the use of DC polarography and differential pulse polarography. Thus the formation constant of copper complex was $10^{3.51}$. Copper (Ⅱ) ion was found to form complex of 1-to-1 composition with [16-ane-$S_4$]. In addition, reduction step was irreversible and the reduction current was diffusion controlled. And the effect of concentration of the salting-out reagent and chelating agent and pH of aqueous phase on the determination of copper (Ⅱ) was investigated and diverse ion effect was discussed. By salting-out extraction technique, we can be determined until the concentration of copper (Ⅱ) of 60 ppb.

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Validation of an Unsteady Two-dimensional Hydrodynamic and Transport Model with Experiments (비정상상태 하천흐름에서 오염물질 혼합 수치모형의 신뢰성 평가)

  • Moon, Hyoung-Bu
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.11
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    • pp.1168-1179
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    • 2006
  • The validity of an unsteady two-dimensional(2-D) numerical hydrodynamic and pollutant dispersion model(2DNHPDM) was evaluated using the data obtained from I-sa streams in Sooncheon, Chonnam, during rain-fall run-off. Field observations was conducted for 35 hours during the 10 hours rainfall event on 7th May 2005. The water level, 2-D velocity, flow field, and COD at seven points selected along the river were measured at intervals of one hour. The model was applied to describe two-dimensional movement of dissolved pollutants in meandering non-uniform river. Major physical processes affecting the lateral and horizontal mixing of the river flow were simulated. The model was proved effective in describing the hydrodynamics and dispersion of the river pollutants from its major tributaries as well as non-point sources.

An Investigation for Driving Behavior on the Exit-ramp Terminal in Urban Underground Roads Using a Driving Simulator (주행 시뮬레이터를 활용한 도심 지하도로 유출연결로 접속부 주행행태 분석)

  • Jeong, Seungwon;Song, Minsoo;Hwang, Sooncheon;Lee, Dongmin;Kwon, Wantaeg
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.21 no.1
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    • pp.123-140
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    • 2022
  • Even though driving behaviors in underground roads can be significantly different from ground roads, existing underground roads follow the design guidelines of ground roads. In this context, this study investigates the driving behaviors of the exit-ramp terminal of urban underground roads using a driving simulator. Virtual driving experiments were performed by analyzing scenarios between the underground and ground road environments. The experiments' driving behavior data for each geometry section are compared and validated through a statistical significance test. This test showed that the speed in the underground road environment is relatively low, and the LPM tends to move away from the adjacent tunnel wall. Based on these findings, this study suggests implications and feasible solutions for improving driver's safety in the exit-ramp terminal of the underground roads.

A Study on User Satisfaction Evaluation of Acceleration-Based Automated Driving Patterns (가속도 기반 자율주행 패턴에 대한 이용자 만족도 평가 연구)

  • Sooncheon Hwang;Dongmin Lee
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.22 no.6
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    • pp.284-298
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    • 2023
  • With the rapid advances in automated driving technology, opportunities to experience automated driving directly or indirectly are being provided to the public. On the other hand, research on the preferred automated driving patterns from the user's perspective has not been conducted in Korea. This study used a driving simulator and an experimental vehicle capable of automated driving to evaluate the user satisfaction regarding longitudinal and lateral accelerations. Automated driving patterns were implemented in a virtual environment simulation using five values of longitudinal and lateral accelerations derived from driving experiments. Among these values, three were implemented through experimental vehicle-based automated driving to evaluate satisfaction and anxiety. The participants evaluated lateral acceleration more sensitively than longitudinal acceleration and showed higher levels of anxiety. Based on these results, the necessity of user-oriented evaluation research for automated driving patterns and the suitability of simulator-based evaluation methods were presented.

Estimation of Driving Behavior Characteristics through Self-Reported-Based Driving Propensity (자기보고 기반 운전성향을 통한 주행행태 특성 추정 연구)

  • Sooncheon Hwang;Dongmin Lee
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.23 no.1
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    • pp.26-41
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    • 2024
  • To ensure safer road conditions, understanding the human factors influencing driving behavior is crucial. However, there are many difficulties in deriving the characteristics of individual human factors that affect actual driving behaviors. Therefore, this study analyzes self-reported dangerous-driving propensities in order to explore potential correlations with drivers' behaviors. The goal is to propose a method for assessing driving tendencies based on varying traffic scenarios. The study employed a questionnaire to gauge participants' propensity to drive dangerously, utilizing a simulator to analyze their driving behaviors. The aim is to determine any notable connections between dangerous-driving propensity and specific driving behaviors. Results indicate that individuals exhibiting a high propensity for reckless driving, as identified by the Korean DBQ, tend to drive at higher speeds and display more aggressive acceleration patterns. These findings contribute to a potential method for assessing reckless driving drivers.

Changes of Surface Temperature and Electromyography Activities by Local Heat and Cold (온열과 냉의 국소적용에 의한 체표면 온도와 근전도 활동의 변화)

  • Choi, Seok-Ju;Lim, Sang-Wan;Kim, Su-Hyon;Mun, Dal-Ju
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.5 no.2
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    • pp.61-72
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    • 2007
  • INTRODUCTION: Local heat and cold application has been frequently used as means of muscle relaxation and blood circulation or reinforcing muscle strength, relaxing muscle tension in clinical situation. In particular, it has been known that long-term heat and cold application for relaxing muscle tension inhibits muscle spasticity or tension. But, it has been rarely reported that what influences of heat and cold application on activation of muscle action potential. Therefore, this study aims to analyze surface temperature and electromyography activities according to the heat and cold application. METHODE: Subjects of this research were 10 normal men and women (5 men, 5 women). Hot pack and cold pack was applied to vastus medialis muscle of thigh and rectus femoris muscle for 20 min. Surface temperature of vastus medialis muscle and rectus femoris muscle was measured, knee joint of subjects was in $45^{\circ}$ flexion, sitting on a chair, maximal isometric contraction was induced, surface electromyography (sEMG) signals were collected and root mean square (RMS) and median frequency (MOF) were analyzed. All measurements were conducted before and immediately after experiment, 10 min., 20 min. and 30 min. after experiment. Data were analyzed with SPSS 12.0 program, comparison of changes in superficial temperature and sEMG signals through repeated measurement was conducted with repeated measures ANOVA and significance level $\alpha$ was 0.05. RESULTS: Changes of surface temperature of vastus medialis muscle according to cold application were radically decreased immediately after application, but it was recovered after 30 min. of application and it showed significant difference (F4. 36=72.216, P<0.001). Surface temperature of rectus femoris also showed radical decrease immediately after application, but it was recovered after 30 min. of application and showed significant difference (F4. 36=88.930, P<0.001). Changes of surface temperature of vastus medialis muscle according to heat application were radically increased immediately after application, but it was recovered after 30 min. of application and it showed significant difference (F4. 36=27.267, P<0.001). Surface temperature of rectus femoris also showed radical decrease immediately after application, but it was recovered after 30 min. of application and showed significant difference (F4. 36=19.774, P<0.001). Changes of sEMG by heat and cold application were no statistical difference. Surface temperature of skeletal muscle after heat and cold application showed significant change for 30 min., but it was found that increase or decrease of surface temperature had not great influence on sEMG activities.

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Synthesis of Li1.6[MnM]1.6O4 (M=Cu, Ni, Co, Fe) and Their Physicochemical Properties as a New Precursor for Lithium Adsorbent (Li1.6[MnM]1.6O4(M=Cu, Ni, Co, Fe)의 합성 및 리튬 흡착제용 신규 전구체로서의 물리화학적 성질)

  • Kim, Yang-Soo;Moon, Won-Jin;Jeong, Soon-Ki;Won, Dae-Hee;Lee, Sang-Ro;Kim, Byoung-Gyu;Chung, Kang-Sup
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.10
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    • pp.4660-4665
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    • 2011
  • New precursors as a Li adsorbent, $Li_{1.6}(MnM)_{1.6}O_4$ (M=Cu, Ni, Co, Fe), were synthesized by hydrothermal method and their physicochemical properties were discussed. XRD and HRTEM results revealed that the original spinel structure was stabilized by cobalt-doping while Cu-, Ni- and Fe-doping led to structural changes. Such a structural stabilization by Cobalt-doping was maintained after lithium leaching by acid treatment. Li absorption efficiency from seawater was significantly enhanced by using the Cobalt-doped spinel manganese oxide, $Li_{1.6}[MnCo]_{1.6}O_4$, compared to the commercially available $Li_{1.33}Mn_{1.67}O_4$; the adsorbed amount of Li from 1g-adsorbent was 35 and 16 mg by $Li_{1.6}[MnCo]_{1.6}O_4$, and $Li_{1.33}Mn_{1.67}O_4$, respectively.

Measurement of the Environmental Value of the Seomjin-River Estuary (섬진강 하구의 환경가치 추정)

  • Yoo, Seung-Hoon
    • Journal of Environmental Policy
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    • v.6 no.2
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    • pp.1-25
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    • 2007
  • This paper attempts to measure the environmental value of the Seomjin-River estuary that has been comparatively well conserved but is confronted with the threat of its development. Especially, in order to elicit the environmental values of its four attributes, contingent valuation method(CVM) based on multi-attribute utility theory is applied and the CVM survey was rigorously designed to comply with the guidelines. for best-practiced CVM studies. We surveyed a randomly selected sample of 300 and 350 households in the Seomjin-River estuary-neighboring area(Gwangyang, Sooncheon, Yeosu, Hadong, Namhae) and seven large cities(Seoul, Busan, Incheon, Daegu, Daejeon, Gwangju, Ulsan), respectively and asked respondents questions in person-to-person interviews about what they would willing to pay for the estuary conservation and management program. Respondents overall accepted the contingent market and were willing to contribute a significant amount(5,763 won in the Seomjin-River estuary-neighboring area and 1,883 won in seven large cities), on average, per household per year, which implies that there is a large difference between the two. The aggregate values of the Seomjin-River estuary in the estuary-neighboring area and seven large cities amount to 1.52 and 14.05 billion won, respectively, per year. The quantitative values can be utilized in planning and decision-making about development versus conservation of the estuary.

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