• Title/Summary/Keyword: soluble stability

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Functional properties of protein from defatted sesame meal using the enzyme from Bacillus sp. CW-1121 (Bacillus sp. CW-1121이 생성하는 효소를 처리한 참깨박 단백질의 기능성)

  • Choi, C.;Chun, S.S.;Cho, Y.J.
    • Applied Biological Chemistry
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    • v.36 no.3
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    • pp.172-177
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    • 1993
  • To extract insoluble proteins from sesame meal residue by microorganism, the sesame meal residue was treated with crude enzyme solution of Bacillus sp. CW-1121. The foaming capacity of salt soluble protein was quite lower than that of water soluble protein and the foaming stability of salt soluble protein decreased abruptly in 10 min., while it sustained for 30 min in case of water soluble protein. Emulsion capacities of all the protein fractions showed minimum value near isoelectric point of protein and salt soluble protein had lower emulsion capacities than that of water soluble protein. The emulsion stability of the protein was relatively stable for 30 min at $80^{\circ}C$. Oil and water absorption capacities of salt soluble protein were higher than those of water soluble protein.

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Effect of Size Distribution of Oil Particles with Emulsifiers and Stabilizers on the Emulsion Stability of Mayonnaise (유화.안정제의 종류에 따른 지방구의 입도 분포가 Mayonnaise의 유화안정성에 미치는 영향)

  • 이영엽
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.2
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    • pp.204-209
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    • 2001
  • The effects of size distribution of oil particles on the emulsion stability of mayonnaise were studied as follows; The stability of mayonnaise has concerned closely with the viscosity and the size distribution of oil particles. Mostly, if the viscosity was increased, the stability was improved, and the distribution of oil particles was uniform and the less the variation, the more the stability. 75% of oil concentration of sample showed the highest viscosity, also the size of sample was the most uniform, compared to other concentration. Mayonnaise prepared with whole egg was unstable, and the size of oil particles was double larger than the case prepared with only the yolk. Addition of xanthan gum increased, the viscosity and the stability by centrifuge so that the more stable mayonnaise could be prepared. The result of using log-normal density function by Heldmann represented that the normal size of sample adding 0.6%-soluble starch and sample N in non-adding control was increased, while those of sample adding xanthan gum and soluble starch at the same time didn't change.

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Microencapsulation of Water-Soluble Isoflavone and Physico-Chemical Property in Milk

  • Seok, Jin-Seok;Kim, Jae-Soon;Kwak, Hae-Soo
    • Archives of Pharmacal Research
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    • v.26 no.5
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    • pp.426-431
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    • 2003
  • This study was carried out to investigate the addition of water-soluble isoflavone into milk by means of microencapsulation technique. The yield of microencapsulation, sensory attributes, and capsule stability of water-soluble isoflavone microcapsules in milk were measured. Coating materials used was polyglycerol monostearate (PGMS), and core material was water-soluble isoflavone. The encapsulation yield of water-soluble isoflavone with PGMS was 67.2% when the ratio of coating material to core material was 15 : 1. The rate of water-soluble isoflavone release from capsules was 18, 19, and 25% when stored at 4,20, and $30^{\circ}C$ for 12 days in milk, respectively. In sensory evaluation, beany flavor and color of microencapsuled water-soluble isoflavone added milk were significantly different from uncapsuled water-soluble isoflavone added milk, however, bitterness was not significantly different. In vitro study, micro-capsules of water-soluble isoflavone in simulated gastric fluid with the range of 3 to 6 pHs were released 3.0∼15.0%, however, the capsules in simulated intestinal fluid with pH 7 were released 95.7% for 40 min incubation time. In conclusion, this study provided that PGMS as coating materials was suitable for the microencapsulation of water-soluble isoflavone, and the capsule containing milk was almost not affected with sensory attribute.

Effect of Water-Soluble Polymer on the Properties of High Strength Hardened Cement Paste (고강도 시멘트 경화체의 특성에 미치는 수용성 폴리머의 영향)

  • 김정환;최상흘;한기성
    • Journal of the Korean Ceramic Society
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    • v.26 no.5
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    • pp.698-704
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    • 1989
  • Basic investigation for the flexural strength and water stability of hardened cement pastes using ordinary portland cement with water-soluble polyer (hydroxypropyl methyl cellulose ; HPMC) was carried out with 0.2 of water cement ratio. For molding of the specimen, the paste was mixed by twin roll mill. According to increase in the content of HPMC, the setting time of cement paste was delayed and the flexural strength was increased. The maximum flexural strength of hardened cement paste with 5.0wt% of HPMC was about 330 kg/$\textrm{cm}^2$. The expansion of the hardened cement paste immersed in water was increased with the content of water soluble polymer(HPMC). Consequently, the strength and the water stability of the hardened cement pastes were remarkably reduced by the expansion of them.

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Development of Water Soluble Tocopherol Emulsion Using Surfactants (계면활성제를 이용한 수분산 Tocopherol 유화물의 개발)

  • Lee, Eun-Hyun;Chang, Kyu-Seob;Lee, Kyong-Haeng
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.11
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    • pp.1465-1471
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    • 2008
  • This study was carried out to make water-soluble tocopherol emulsion which can be applicable directly in water. The molecular weight of tocopherol was 340 to 360 and tocopherol emulsion model was decided as O/O/W/W type. In correlation between stability of emulsion and surface tension, the stability in surface tension of emulsion was from 40 to 46 dyne/cm. In the case of lower than 40 dyne/cm of surface tension, the stability of the emulsion was lower. Lipophilic surfactants, especially for a polyglycerine polyricinoleate in 20%, 30% and 40% tocopherol emulsion, was the most effective in emulsion stability. A higher stability of the emulsion among hydrophilic surfactants in the tocopherol emulsion was obtained in the following order; polyglycerine monostearate> polyglycerine monooleate> polyoxyethylene (20) sorbitan monooleate$\geq$ polyoxyethylene (20) sorbitan monolaurate.

Vacuum Pressure Treatment of Water-Soluble Melamine Resin Impregnation for Improvement of Dimensional Stability on Softwoods (목재의 치수안정성 개선을 위한 진공가압 멜라민 수지함침처리)

  • Oh, Seung-Won;Park, Hee-Jun
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.3
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    • pp.327-333
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    • 2015
  • To measure the dimensional stability of three softwoods by vacuum pressurization of water soluble melamine-formaldehyde (MF) resin impregnation process, properties of resin treated compressed woods (compregs) were measured after impregnating the water soluble MF resin into three coniferous wood species for different impregnation times and resin concentrations. As the resin concentration was higher and impregnation time was longer, coefficients of volumetric shrinkage and anti-volumetric swelling efficiency increased, but coefficients of volumetric swelling, anti-volumetric swelling efficiency and absorption decreased. Also, weight percent gain increased remarkably as the resin concentration was high, but there was no uniform relationship with impregnation time.

A study on the Physico-chemical Properties of CB-ph. a New Anti-cancer drug

  • Kim, Su-Yoen;Kim, Dae-Duk;Lee, Chi-Ho
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.413.1-413.1
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    • 2002
  • Purpose To investigate the physico-chemical properties of CB-ph [2-benzoyloxycinnamaldehyde], an anticancer drug obtained from Cinnamomum cassia using methylenechloride. and its stability in various aqueous solutions. Results CB-ph was rarely soluble in water but soluble in methanol and very soluble in ether. Kinetic salt effect on degradation of CB-ph in buffer solutions at pH 4.0 and 6$0^{\circ}C$ showed a linear relationship having a positive slope that means reactions between hydronium ions and protonated substrates. (omitted)

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Synthesis of Water Soluble Acrylic Modified Epoxyester Resin and Physical Properties of Coatings (수용성 아크릴 변성 에폭시에스테르 수지의 합성 및 도막물성)

  • Kim, Seong-Kil;Shin, Pan-Woo;Lee, Dong-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.28 no.1
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    • pp.35-47
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    • 2011
  • For the synthesis of water soluble acrylic modified epoxyester resin, fatty acid/epoxy ratio of 50/50 was used, and introduced maleic anhydride. Ratio of styrene/acrylic acid of acrylic monomers was fixed 85/15 and ratio of epoxyester/acrylic monomer was controlled 80/20, 75/25, 70/30, 65/35, and degree of neutralization were changed 65%, 80%, to 100%. As a result, 40% solids acrylic modified epoxyester resins were synthesized. Resins were evaluated water soluble stability, drying time, water resistant, storage stability and physical properties. And the white paints were prepared, and were evaluated viscosity, drying time, water resistance, adhesion, sagging, spray workability, gloss, salt spray resistance, skinning, whiteness and flash rust. As a result, the degree of neutralization of 100% and the ratio of epoxyester/acrylic monomer of 75/25 showed the best properties.

Studies on Immobilized Alkaline Protease (고정화 Alkaline Protease 에 관한 연구)

  • 전문진;심상국;정동효
    • Microbiology and Biotechnology Letters
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    • v.6 no.1
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    • pp.33-40
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    • 1978
  • Immobilization of alkaline protease was investigated by absorbing the enzyme on adsorbents. Alkaline protease was adsorbed on silica gel selected as a carrier to immobilize the enzyme. In this study, properties of the immobilized enzyme were compared with those of the soluble enzyme. 1) The optimum pH (10.0) of the enzyme was not changed, but the activity was increased at alkaline pH by immobilization. 2) The optimum temperature of the immobilized enzyme was shifted from 50$^{\circ}C$ to 45$^{\circ}C$, while the temperature-activity Profile became broader than those of the soluble enzyme. 3) The pH stability of the immobilized enzyme was significantely increased at pH 4.0, althouth it did not change in the neutral and alkaline pH region. 4) The heat stability of the enzyme was enhanced in the temperature range of 55$^{\circ}C$∼65$^{\circ}C$ by the immobilization. 5) The immobilized enzyme retained 40% of its original activity after repetitive use for 6 times. 6) The enzyme stability was greately improved for a prolonged storage at 4$^{\circ}C$.

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Stability and Cytotoxicity of Fab-Ricin A Immunotoxins Prepared with Water Soluble Long Chain Heterobifunctional Crosslinking Agents

  • Woo, Byung-Ho;Lee, Jung-Tae;Park, Myung-Ok;Lee, Kang-Ro;Han, Jeung-Whan;Park, Eun-Seok;Yoo, Sun-Dong;Lee, Kang-Choon
    • Archives of Pharmacal Research
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    • v.22 no.5
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    • pp.459-463
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    • 1999
  • The effects of the hindered and non-hindered water soluble long-chain disulfide bonds on the stability and cytotoxicity of the ricin A chain (RTA) immunotoxin were examined. The RTA immunotoxins were prepared with the Fab fragments of anti-common acute lymphoblastic leukemia antigen (CALLA) monoclonal antibody (Fab-RTA) using sulfosuccinimidyl-6-[(-methyl-(-2-pyridyldithio)toluamido]toluamido]hexanoate (S-LC-SMPT) and sulfosuccinimidyl-6-[3-(2-pyridyldithio-propionamido]hexanoate (S-LC-SPDP). The prepared Fab-RTA immunotoxins were evaluated for their conjugation yield, immunoreactivity, thermal and disulfide bond stability and cytotoxicity. The conjugation yield of the Fab-RTA immunotoxin from the water soluble long chain crosslinking agents, S-LC-SMPT and S-LC-SPDP, were comparable. Both Fab-RTA immunotoxins exhibited a similar immunoreactivity and thermal stability in aqueous solution. However, S-LC-SMPT -mediated Fab-RTA, sterically hindered, showed an enhanced disulfide bond stability in vitro over S-LC-SPDP mediated one. In the cytotoxicity against antigenic cell Daudi, the S-LC-SMPT -mediated RTA immunotoxin maintained a comparable cytotoxicity, compared with S-LC-SPDP mediated Fab-RTA immunotoxin.

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