• Herbal Formula Science
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• v.7 no.1
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• pp.183-188
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• 1999

Though development of diverse and highly value-added commodities in Artemisia Iwayomogi, we can expect such effects as procurement of secure farm's production bases and promotion of consumer's demands. Thus, as a first step for development of funtional foods of Artemisia Iwayomogi, solids yield, physical properties and scopoletin contents which is main component of Artemisia Iwayomogi, were investigated according to extration solvents and temperatures. The main result of this experiment were as following: solid yield in 50% ethanol extracts showed higher than those of water extracts at the same temperature. In condition of 50% ethanol extracts, solid yield, degree of browing and scopoletin contents showed increasing, but turbidities which mean transmittance(%T) showed decreasing sa temperature rise. In water extracts, pH values showed increasing as temperature rise.

• Korean Journal of Agricultural Science
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• v.21 no.1
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• pp.45-53
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• 1994

This study was conducted to estimate the genetic and environmental effects on lactation yield and milk compositions in Holstein cows. The data analysis were the records of 159 cows rearing at Nation Animal Breeding Institute from 1990 to 1991. The least square means were estimated on milk and fat yield in lactation yield, and the percent of fat, protein, solids-not-fat and total solid in milk composition. The results obtained are summarized as follows: 1. The average yield of milk and fat in 305 days were $7,26.56{\pm}3,57.24$ and $254.65{\pm}44.94Kg$ and the percent of fat, protein, solids-not-fat and total solid were $3.69{\pm}0.43$, $3.32{\pm}0.41$, $9.15{\pm}0.49$ and $12.75{\pm}0.96$ and the coefficients of variation were 18.68, 17.64, 11.88 and 12.34% for milk yield, fat yield, fat percent and protein percent, respectively. 2. The effect of sires was highly significant at 1% level in milk and fat yield and fat percent, and significant at 5% level in protein and total solid percent. Among the sires, B, L and O sire were superior in milk yield with 7,571.22, 7,499.11 and 7,420.58 Kg, and A, F and K sire were superior in protein percent with 3.75, 3.64 and 3.65, respectively. 3. The effect of parity was highly significant at 1% level in milk yield, and significant at 5% level in fat yield. Among the parities, the 3rd parity was superior in milk and fat yield with 7,634.54 and 274.98 Kg, and the 4th and over was superior in fat and protein percent with 3.90 and 3.50, respectively. 4. The effect of calving seasons was highly significant at 1% level in milk yield, and significant at 5% level in fat yield, and the percent of fat, protein and total solid. Among the calving seasons, spring and winter were superior in milk yield with 7,310.31 and 7,364.57 Kg, also spring and winter were superior in protein percent with 3.68 and 3.52, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.1
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• pp.78-84
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• 2005

The purpose of this study was to optimize the solid state cultivation of Monascus ruber on sterile rice. A single-level-multiple-factor and a single-factor-multiple-level experimental design were employed to determine the optimal medium constituents and to optimize carbon and nitrogen source concentrations for lovastatin production. Simultaneous quantitative analyses of the ${\beta}$-hydroxyacid form and ${\beta}$-hydroxylactone for of lovastatin were performed by the high performance liquid chromatography (HPLC) method with a UV photodiode-array (PDA) detector. The total lovastatin yield ($4{\sim}6\;mg/g$, average of five repeats) was achieved by adding soybean powder, glycerol, sodium nitrate, and acetic acid at optimized levels after 14 days of fermentation. The maximal yield of lovastatin under the optimal composition of the medium increased by almost 2 times the yield observed prior to optimization. The experimental results also indicated that the ${\beta}$-hydroxylactone form of lovastatin (LFL) and the ${\beta}$-hydroxyacid form of lovastatin (AFL) simultaneously existed in solid state cultures of Monascus ruber. while the latter was the dominant form in the middle-late stage of continued fermentation. These results indicate that optimized culture conditions can be used for industrial production of lovastatin to obtain high yields.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.1
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• pp.1-5
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• 2002

A fusion protein, consisting of a human epidermal growth factor (hEGF) as the recognition domain and human angiogenin as the toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as inclusion body in recombinant E. coli, and when the conventional, solution-phase refolding process was used the refolding yield was very low due to severe aggregation. It was probably because of the opposite electric charge at a neutral pH resulting from the vastly different pI values of each domain. The solid-phase refolding process that exploited the ionic interactions between ionic exchanger surface and the fusion protein was tried, but the adsorption yield was also very low, below $ 30\%$, regardless of the resins and pH conditions used. Therefore, to provide a higher ionic affinity toward the solid matrix, six lysine residues were tagged to the N-terminus of the hEGF domain. When heparin-Sepharose was used as the matrix, the adsorption capacity increased 2.5-3 times to about $88\%$. Besides the intrinsic affinity of angiogenin to heparin, the poly-lysine tag provided additional ionic affinity. And the subsequent refolding yield increased nearly 13-fold, from ca. $4.8\%$ in the conventional refolding of the untagged fusion protein to $63.6\%$. The process was highly reproducible. The refolded protein in the column eluate retained RNase bioactivity of angiogenin.

• Korean Journal of Materials Research
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• v.24 no.4
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• pp.201-206
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• 2014

TiC-21 mol% Mo solid solution (${\delta}$-phase) and TiC-99 mol% Mo solid solution (${\beta}$-phase), and TiC-(80~90) mol% Mo hypo-eutectic composite were deformed by compression in a temperature range from room to 2300 K and in a strain rate range from $4.9{\times}10^{-5}$ to $6.9{\times}10^{-3}/s$. The deformation behaviors of the composites were analyzed from the strengths of the ${\delta}$- and ${\beta}$-phases. It was found that the high strength of the eutectic composite is due primarily to solution hardening of TiC by Mo, and that the ${\delta}$-phase undergoes an appreciable plastic deformation at and above 1420 K even at 0.2% plastic strain of the composite. The yield strength of the three kinds of phase up to 1420 K is quantitatively explained by the rule of mixture, where internal stresses introduced by plastic deformation are taken into account. Above 1420 K, however, the calculated yield strength was considerably larger than the measured strength. The yield stress of ${\beta}$-phase was much larger than that of pure TiC. A good linear relationship was held between the yield stress and the plastic strain rate in a double-logarithmic plot. The deformation behavior in ${\delta}$-phase was different among the three temperature ranges tested, i.e., low, intermediate and high. At an intermediate temperature, no yield drop occurred, and from the beginning the work hardening level was high. At the tested temperature, a good linear relationship was held in the double logarithmic plot of the yield stress against the plastic strain rate. The strain rate dependence of the yield stress was very weak up to 1273 K in the hypo-eutectic composite, but it became stronger as the temperature rose.

• Korean Journal of Food Science and Technology
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• v.31 no.5
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• pp.1211-1215
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• 1999

The solid yields and color changes of garlic aqueous extracts were determined to study the effects of enzymatic hydrolysis, pH treatment, and addition of organic acids and some organic or inorganic salts. Hydrolysis with commercial polysaccharases resulted in an $15{\sim}20%$ increase in the solid yields, with a greenish color change in the garlic extracts. pH treatment gave it similar effect at the pH range of $4.4{\sim}5.0$. Among the salts and acidulants used, $CaCl_2$, sodium oxalate, tartaric and phosphoric acids were effective for increase in the yield and $K_{4}P_{2}O_{7}$ was effective for both yield increase and control of greening.

• Journal of Applied Biological Chemistry
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• v.55 no.3
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• pp.149-156
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• 2012

The effects of solvent type and concentration, solid/liquid ratio, extraction time and repeated extraction on recovery of keratinase from solid-state fermentation (SSF) of chicken feather by a local Streptomyces sp. NRC 13S were investigated in order to establish the experimental conditions for keratinase yield. Among solvents tested, 0.5% (v/v) glycerol was the best. Box-Behnken design was used to investigate the effect of relevant variables on keratinase recovery. The factors investigated were solid/liquid ratio (1:1.66-1:6.66 g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The results showed that the maximum recovery of keratinase (6933.3 U/gfs) was obtained using 0.5 (v/v) glycerol as extracting solvent, in a solid/liquid ratio of 1:5 and three extraction cycles.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1997.06a
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• pp.5-15
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• 1997

It is the objective of this study that by conducting the serni-solid extrusion using A12024, the effect of various process variables on the quality of extruded product and extrusion force is understood. The results of experiment are compared with those of finite element simulation in order to verify the effectiveness of the developed FE-simulation code. In order to simulate densification in the deformation of serni-solid material, the semi-solid material is assumed to be composed of solid region as porous skeleton following compressible visco-plastic model and liquid region following Darcy's equation for the liquid flow saturated in the interstitial space. Then the flow and deformation of the semi-solid alloy are analyzed by coupling the deformation of the porous skeleton and the flow of the eutectic liquid. It is assumed that initial solid fraction is homogeneous. Yield and plastic potential function presented by Kuhn and constitutive model developed by Gunasekera are used for solid skeleton.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.392-396
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• 2010

This research focused on determining the effectiveness of cluster and/or flower thinning in highbush 'Jersey' blueberry on the yield and fruit quality. The total yield and quality of fruit were compared between thinning methods of control (no thinning), 1/3 cluster thinning, 1/3 cluster thinning + 1/2 flower thinning, 2/3 cluster thinning, and 2/3 cluster thinning + 1/2 flower thinning per each bearing shoot, respectively. Thinning strength significantly affected the yield of bearing shoot and, thus, total yield of control and 1/3 cluster thinning was higher than others. Both cluster and flower thinning, however, significantly affected the fruit growth resulting in the increase of fruit weight, length and width. Even total yield was similar between control and 1/3 cluster thinning, more larger fruit (> 1.6 g) were produced by 1/3 cluster thinning treatment, indicating that flower and/or cluster thinning contributed to the increase of individual fruit growth. Unlike fruit growth, fruit quality was less affected except total soluble solid contents. Total soluble solid level significantly increased in treatments (2/3 cluster thinning, 2/3 cluster thinning + 1/2 flower thinning) with yield decrease. No significant difference in levels of acidity and firmness of fruit was found. Results indicated that fruit growth and total yield of highbush blueberry was more influenced by the thinning strength regardless of cluster or flower than internal quality of fruit such as solid and acid levels.

• Journal of Bio-Environment Control
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• v.25 no.4
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• pp.283-287
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• 2016

This study was conducted to examine the effects of defoliation levels during winter season on the yield and soluble solid contents of fruits. To achieve this strawberry 'Seolhyang' cultivated forcingly in the year of 2013 to 2014 as well as 2014 to 2015. The three treatments include periodical removing of leaves remaining 6 fully expanded leaves, those remaining 9 leaves, and only removing of old and infected leaves during the period from early December to end of February. In the early marketable fruit yield until the end of February and total marketable fruit yield, the treatment of non-defoliation was significantly higher than those of the treatment of 6 and 9 leaves in the year of 2014 to 2015 cropping season. Soluble solid contents of fruits among January and March increased significantly as the number of leaves get higher. Soluble solid contents were higher and stable around $12^{\circ}Brix$ in the treatment of non-defoliation. Assimilation of photosynthetic products into plant tissue are insufficient due to the lack of irradiation and short day in winter season forcing cultivation of strawberry. Thus, it is considered to manage the leaves with defoliation for only old and infected leaves from December to February for increasing of fruit yield and improvement of fruit quality.