• 제목/요약/키워드: solid carbon sources

검색결과 73건 처리시간 0.025초

Glucoamylase Production in Batch and Fed-Batch Solid State Fermentation: Effect of Maltose or Starch Addition

  • Bertolin, Telma Elita;Jorge Alberto Vieira Costa;Gean Delise Leal Pasquali
    • Journal of Microbiology and Biotechnology
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    • 제11권1호
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    • pp.13-16
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    • 2001
  • Maltose and soluble starch were used as secondary sources of carbon for glucoamylase production by Aspergillus awamori in solid state fermentation. During batch cultivation, maltose above 2.5%(w/w) repressed glucoamylase production, but, by adding either 2.5% (w/w) maltose or 1.25% (w/w) soluble starch to fed-batch cultivations, glucoamylase activity was increased by 15% and 170% over standard medium, respectively. The data showed that maltose is a weak inducer of glucoamylase production in solid stat fermentation.

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Production of Red Pigments by Monascus purpureus in Solid-state Culture

  • Lee Bum-Kyu;Piao Hai Yan;Chung Wook-Jin
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제7권1호
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    • pp.21-25
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    • 2002
  • To maximize and sustain the productivity of Monascus pigments, various environmental and nutritional parameters, such as the initial moisture content, pH, inoculum size, sample size, and nutrient supplement, that influence pigment production were evaluated in solid-state cultures as follows: initial moisture content, $50\%;$ pH, 6.0; inoculum size $1\;\times\;10^4$ spore cells $(grams\;of\;dry\;solid\;substrate)^{-1};$ sample size, 300 g. All supplementary nutrients (carbon, nitrogen, and mineral sources) added has inhibitory effects on the cell growth and red pigment production. In open tray culture the maximum biomass yield and specific productivity of red pigments were 223 mg DCW $(grams\;of\;initial\;dry\;substrate)^{-1}$ and, $47.6\;OD_{500}\;(DCW\;grams)^{-1}h^h{-1}$ respectively.

외부 유입 가스 조절을 통한 연료전지 구동 성능 안정화 (An Experimental Guide to Predictable Fuel Cell Operations by Controlling External Gas Supply)

  • 장한샘;박영은;이재영
    • 공업화학
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    • 제29권5호
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    • pp.626-629
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    • 2018
  • 연료전지는 높은 연료유연성을 갖고 있어, 탄소 및 탄화수소 등을 통해서도 전기를 생산할 수 있다. 하지만 이러한 연료원을 사용한 경우, 불안정한 장기 구동성능이 종종 관측된다. 본 연구에서는 반응기 내부에 존재하는 탄화수소가 장기 구동성능을 불안정하게 함을 밝힌다. 본 연구진은 비활성기체인 아르곤을 이용하여 산화극 반응기 내부의 가스화경로를 예측하고, 이를 통해 탄화수소의 영향을 억제하여 불안정한 장기 구동성능을 극복한다. 더 나아가, 산화극 반응기 내부에 이산화탄소를 공급하여 역부드아반응을 유도한다. 역부드아반응을 통해 탄소연료전지에서 연료로 사용될 수 있는 일산화탄소를 만들어낸다. 과도한 이산화탄소의 주입은 오히려 연료손실 등의 문제를 야기함을 확인한다. 따라서, 본 연구에서는 이산화탄소 공급량의 최적화가 중요함을 밝히고, 이를 통해 연료전지 구동 성능을 안정화한다.

Aspergillus sp.의 Glucoamylase 생산에 미치는 요인 (Some Factors Affecting Glucoamylase Production from Aspergillus sp.)

  • Park, Inshik;Youngho Chung
    • 한국미생물·생명공학회지
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    • 제17권5호
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    • pp.519-523
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    • 1989
  • Aspergillus sp.로부터 glucoamylase를 생성키 위한 조건을 검토하였다. 곰팡이는 탄소원으로 soluble starch, 질소원으로 yeast extract를 사용했을 때 최대 효소생성을 얻을 수 있었다. 그리고, 탄소원 및 질소원의 농도에 따른 효소생성은 soluble starch를 5%, yeast extract를 1% 수준으로 사용했을 때 효소생성은 극대화하였다. 또한 배지의 초기 pH를 6.0, 그리고 배양온도를 28$^{\circ}C$로 유지했을 때 효소생성이 높았다. 고체배지를 사용했을 때에는, 밀기울 배지가 glucoamylase 생성을 위해서 가장 효과가 좋았다.

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Characterization of SiC/C Nanocomposite Powders Synthesized by Arc-Discharge

  • Zhou, Lei;Yu, Jie Yi;Gao, Jian;Wang, Dong Xing;Gan, Xiao Rong;Xue, Fang Hong;Huang, Hao;Dong, Xing Long
    • Applied Microscopy
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    • 제45권4호
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    • pp.242-248
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    • 2015
  • In this paper, three carbon sources, i.e., solid graphite, gaseous CH4 and liquid ethanol, and one solid silicon source were employed to synthesize SiC/C nanocomposite powders by arc-discharge plasma. The processing conditions such as the component ratios of raw materials, atmospheric gases, etc. were adjusted for controllable synthesis of the nanopowders. It is indicated that both of solid graphite and silicon can be co-evaporated and reacted to form nanophases of cubic ${\beta}$-SiC with ~50 nm in mean size and a little free graphite; the carbon atoms decomposed from gaseous $CH_4$ favor to combine with the evaporated silicon atoms to form the dominant SiC nanophase; liquid carbon source of ethanol can also be used to harvest the main ${\beta}$-SiC and minor 6H-SiC phases in the assembly of nanoparticles. The as-prepared SiC/C nanocomposite powders were further purified by a heat-treatment in air and their photocatalytic performances were then greatly improved.

Effects of Temperature and Culture Media Composition on Sporulation, Mycelial Growth, and Antifungal Activity of Isaria javanica pf185

  • Lee, Jang Hoon;Lee, Yong Seong;Kim, Young Cheol
    • 식물병연구
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    • 제27권3호
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    • pp.99-106
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    • 2021
  • The fungal isolate Isaria javanica pf185 has potential as a mycopesticide because it demonstrates insecticidal activity against the green peach aphid and antifungal activity against Colletotrichum gloeosporioides. For commercialization of this isolate, determination of the optimal and least expensive culture conditions is required; however, these data are not currently available. This study describes the conditions for optimal development of conidia and production of metabolites for the biocontrol of the fungal pathogen. The optimal culture conditions were examined using cultures on solid agar and liquid media. High growth temperature enhanced spore formation but reduced antifungal activity in both solid and liquid media. The highest spore yield was obtained in a medium containing glucose as a carbon source and yeast extract as a nitrogen source. Soybean powder and wheat bran were effective nitrogen sources that promoted spore production and antifungal activity of the isolate. These results revealed the basic, cost-effective growth media for commercial production of a biopesticide with insecticidal and antifungal properties for use in integrated pest management.

대구시의 지역별 생활쓰레기 성상 및 특성 (The Physico-chemical Characteristics of Municipal Solid Wastes from Four Different Sources in Daegu City)

  • 노숙현;이동훈;최충렬;박만;박병윤;최정
    • 한국환경과학회지
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    • 제12권4호
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    • pp.461-467
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    • 2003
  • This study was conducted to provide the basic information to know reasonable method f3r the removal and treatment of municipal solid wastes(MSWs). MSWs four different sources(apartment residence, commerce, office/industry) in Daegu city were collected, and bulk density, physical composition, moisture, combustible, ash, elements and heating values were investigated. The values of bulk density were at range of 0.30∼0.41ton/m$^3$. The percentages of foods, papers, vinyls/plastics, textiles, rubbers/leathers, metals and glasses/ceramics of wet muncipal solid wastes from three sources except office/industry were at range of 27.3∼46.4%, 21.2∼38.4%, 10.3∼11.9%, 3.8∼5.2%, 0.5∼1.7%, 6.9∼8.7% and 1.9∼2.6% respectively. The percentages of papers, foods, vinyls/plastics, textiles, rubbers/leathers, metals and glasses/ceramics of dry muncipal solid wastes from three sources except office/industry were at range of 21.1∼40.4%, 14.2∼30.1%, 15.2∼17.8%, 4.0∼6.0%, 0.9∼2.8%, 12.2∼14.1% and 3.1∼3.7% respectively. The moisture contents of foods, woods, textiles and papers in wastes were at range of 65.7∼76.2%, 34.6∼50.0%, 22.2∼44.8 and 30.6∼44.4% respectively. The carbon contents of vinyls/plastics, rubbers/leathers, textiles, woods, papers and foods in wastes were at range of 75.88∼79.87%, 58.24∼70.74%, 45.23∼51.63%, 42.29∼45.58%, 40.19∼48.4% and 25.86∼36.25% respectively. The low heating values of foods, papers, woods, textiles, rubbers/leathers and vinyls/plastics by Dulong's law were at range of 178∼1,353kcal/kg, 770∼1,660kcal/kg, 995∼1,629kcal/kg, 2,133∼2,432kca1/kg, 4,200∼7,275 kcal/kg and 6,384∼8,722kcal/kg respectively, and the high heating values of them were at range of 782∼2,056 kcal/kg, 2,459∼3,314kca1/kg, 3,056∼3,592kcal/kg, 4,381∼5,087kca1/kg, 5,005∼8,066kca1/kg and 10,032∼10,739 kcal/kg respectively.

개암버섯의 균사생장(菌絲生長)에 영향을 미치는 배양조건(培養條件)에 관한 연구(硏究) (Studies of Cultural Condition on the Mycelial Vegetative Growth in Naematoloma sublateritium (Fr.) Karst.)

  • 강안석;차동열;홍인표;장현유;유승헌
    • 한국균학회지
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    • 제22권2호
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    • pp.153-159
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    • 1994
  • 새로운 식용버섯 자원인 개암버섯(N. sublateritium)의 균사생장에 관한 배양적 조건을 구명하고자 시험을 수행한 결과는 다음과 같다. 1. 균사배양에 적합한 온도 및 pH는 각각 $25^{\circ}C$, 5.0-6.0의 범위였다. 2.균사배양시 양호한 탄소원으로서 단당류는 sorbitol, d-ribose 다당류는 starch, inulin, dextrin등이 있다. 3. 질소원으로 양호한 것은 무기태 질소인 질산칼륨, 암모니아태 질소인 asparagine, leucine, phenylalanine 등이었다. 4. 탄소원인 sorbitol 농도를 1-2%로 고정했을 때 적합한 C/N 비는 50이었고 질소원 농도가 높아질수록 균사생장은 부진하였다. 5. 개암버섯 균사생장에 필요한 무기염류는 비교적 다양하였고 $KH_2PO_4,\;MgSO_4{\cdot}7H_2O,\;CaCl_2{\cdot}2H_2O$의 최적농도는 각각 0.08%, 0.03%, 0.01%로 낮은 경향이었다. 6. 유기산으로 양호한 것은 fumaric, maleic, tartaric, gallic acid 등이었다.

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다양한 청록수소 생산 공정에 대한 경제성 분석 (Economic Comparison of Various Turquoise Hydrogen Production Processes)

  • 이수용;잡반티엔;무자히드 나심;김종환;이영덕
    • 한국수소및신에너지학회논문집
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    • 제34권3호
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    • pp.256-266
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    • 2023
  • Hydrogen production can be classified based on the energy source, primary reactor type, and whether or not it emits carbon dioxide. Utilizing color representation proves to be an effective means of expressing these distinctive characteristics. Among the various clean hydrogen production techniques, there has been a growing interest in turquoise hydrogen production, which involves the decomposition of methane or other fossil fuels. This method offers advantages in terms of large-scale production and cost reduction through the sale of solid-carbon byproduct. In this study, an extensive literature review was conducted to select and analyze several promising candidates for turquoise hydrogen production processes. The efficiency and economics of these processes were evaluated using stream data reported in the literature sources. The findings indicate that the levelized cost of hydrogen production (LCOH) is significantly influenced by the sales of byproducts, specifically the solid-carbon and carbon monoxide byproducts.

[ $CO_2$ ] Sequestration on Various Structures of Natural Gas Hydrate Layer for Effective Recovery of $CH_4$ Gas

  • Park, Young-June;Choi, Suk-Jeong;Shin, Kyu-Chul;Seol, Ji-Woong;Lee, Hu-En
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2006년도 춘계학술대회
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    • pp.410-411
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    • 2006
  • On the continental margins and in permafrost regions, natural gas, which has been expected to replace petroleum energy, exists In solid hydrate farm. World hydrate reserves Including natural gas are estimated at about twice as much as the energy contained In total fossil fuel reserves. Because of its vast quantities, the efficient recovery of natural gas from natural gas hydrate becomes the most important factor on evaluating the economic feasibility in the sense of commercialization. It has been noted that carbon dioxide, one of the well-known green house gases, possibly can be stored in the ocean floor as a carbon dioxide hydrate. If the natural gas hydrate could be converted into carbon dioxide hydrate, natural gas hydrate deposits would serve as energy sources as well as carbon dioxide storage sites in the deep ocean sediments. In this study, we first attempted to examine the real swapping phenomenon occurring between guest molecules and various structures of gas hydrate through spectroscopic identification such as NMR spectroscopy.

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