Some Factors Affecting Glucoamylase Production from Aspergillus sp.

Aspergillus sp.의 Glucoamylase 생산에 미치는 요인

The effects of carbon, nitrogen sources and culture conditions on glucoamylase production from Aspergillus sp. were investigated. Among tested carbon sources, soluble starch was most effective for the production of the enzyme, and the level of concentration for the optimal enzyme production was found to be 5%. For nitrogen sources, yeast extract was best for the enzyme production, with the level of 0.1%. The enzyme was maximally produced by cultivating the organism at medium of initial pH 6.0, and temperature of 28$^{\circ}C$. Wheat bran was most suitable for the enzyme production from the organism in solid state culture.

Aspergillus sp.로부터 glucoamylase를 생성키 위한 조건을 검토하였다. 곰팡이는 탄소원으로 soluble starch, 질소원으로 yeast extract를 사용했을 때 최대 효소생성을 얻을 수 있었다. 그리고, 탄소원 및 질소원의 농도에 따른 효소생성은 soluble starch를 5%, yeast extract를 1% 수준으로 사용했을 때 효소생성은 극대화하였다. 또한 배지의 초기 pH를 6.0, 그리고 배양온도를 28$^{\circ}C$로 유지했을 때 효소생성이 높았다. 고체배지를 사용했을 때에는, 밀기울 배지가 glucoamylase 생성을 위해서 가장 효과가 좋았다.