• Journal of Applied Biological Chemistry
• /
• v.54 no.4
• /
• pp.296-302
• /
• 2011

Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-$EC_{50}$ values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.9 no.4 s.44
• /
• pp.29-42
• /
• 2005

Total 350 borehole profiles were selected from the database of borehole logs in Seoul, for the site-specific seismic evaluation at two 4km${\times}$4km plain areas. Equivalent-linear site response analyses for the selected 350 sites were conducted based on shear wave velocity (Vs) Profiles, which were determined from the N-Vs correlation established using borehole seismic testing results in the inland areas of Korea. Most sites were categorized as site classes C and D based on the mean Vs to 30 m in depth (Vs30) ranging from 250 to 550 m/s. The she periods of the plains in Seoul ranging between 0.1 and 0.4 sec were significantly lower than those of the western US, from which the site coefficients in Korea were derived. For plains in Seoul, the site coefficients, Fa's and Fv's specified in the Korean seismic design guide, underestimate the ground motion in short-period (0.1-0.5 sec) band and overestimate the ground motion in mid-period (0.4-2.0 sec) band, respectively, because ol the differences in the geotechnical conditions between Seoul and the western US, although the Fa's in several sites overestimate the motion due to the base Isolation effect resulted from the soft layer in soil deposit.

• Journal of the Korean Geotechnical Society
• /
• v.32 no.8
• /
• pp.5-14
• /
• 2016

The leakage of water under sewer pipelines is one of main sources of sinkholes in urban areas. We performed laboratory model tests to investigate the presence of cavities using impact-echo method, which is a nondestructive test method. To simulate a concrete sewer pipe, a thin concrete plate was built and placed over container filled with sand. The cavity was modeled as an extruded polystyrene foam box. Two sets of tests were performed, one over sand and the other on cavity. A new impact device was developed to apply a consistent high frequency impact load on the concrete plate, thereby increasing the reliability of the test procedure. The frequency and transient characteristics of the measured reflected waveforms were analyzed via fast Fourier transform and short time Fourier spectrum. It was shown that the shapes of Fourier spectra are very similar to one another, and therefore cannot be used to predict the presence of cavity. A new index, termed resonance duration, is defined to record the time of vibration exceeding a prescribed intensity. The results showed that the resonance duration is a more effective parameter for predicting the presence of a cavity. A value of the resonance period was proposed to estimate the presence of cavity. Further studies using various soil types and field tests are warranted to validate the proposed approach.

• Geotechnical Engineering
• /
• v.14 no.5
• /
• pp.163-172
• /
• 1998

To study the carrying capacity behavior of pile, dynamic pile testis and static load tests were carried out on two instrumented piles during and some time after pile driving. Cone Penetration Test( CPT) and Standard Penetration Test(SPT) were also performed at the test site before pile tests to investigate the relationship between unit skin friction of piles and cone tip resistance values and SPT N values. Total static capacity of pile reached the ultimate stage at the pile head settlement of about 0.055D (D : Pile diameter), at which skin friction of Pile already Passed the maximum value, but the end bearing was still increasing with the pile head settlement. The carrying capacity of pile increased in the form of natural logarithmic function with the time after pile driving. The increase in skin friction with time was very substantial the increase in skin friction 40 days after pile driving was 4.6 times of that determined during pile driving. The contribution of skin friction to the total capacity twas insignificant in the beginning, but became substantial 40 days after pile driving. This implies that the tested pile initially responded as an end bearing pile and later behaved as a friction pile. It was also noted that unit skin friction of pile might be ielated to cone tip resistance values(q.) and SPT N values, though the coefficient of this relationship might differ from one soil group to another and was somewhat greater than the value used in the design practice of Korea.

• Journal of the Korean GEO-environmental Society
• /
• v.10 no.7
• /
• pp.7-14
• /
• 2009

The correlation equation empirically proposed to obtain compression indexes has been proposed to conveniently obtain the value using the soil parameter that can be obtained through simple tests when the number of time of consolidation testing is low or the distribution is large but most of the analyzed regions are limited to certain regions abroad or in the country and multiple data were integrated for use in many cases, thus it is not very reasonable to apply it. Therefore, to establish a new design method considering the uncertainty of the ground, it was selected the Kwangyang port area of which the data have been collected recently thus are relatively more reliable as the subject region of the study in order to maximally reduce the uncertainty of test data. After performing the verification of the normality of the consolidation test data obtained from the selected region and the transformation of variables, a prediction formula was proposed through the regression model with the transformed variables and the proposed regression model with transformed variables was compared with existing empirical equations to verify the suitability of the proposed model formula. After analyzing, it was confirmed that the coefficient of determination was increased after the Box-Cox variable transformation, thus the explanatory power was being enhanced and through the root-mean-square-error method, it was confirmed that the proposed model formula showed the most closed value to the test value.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.4
• /
• pp.218-225
• /
• 2020

This study analyzed the priorities of the cross-compliance items of public-benefit direct payment using an Analytic Hierarchy Process and Fuzzy Decision Making Analysis. The valuation criteria are policy efficiency, farm acceptability, and feasibility, and the valuation targets are the basic and additional cross-compliance items. The survey was performed by targeting 50 experts from each class, and conducted for about a month starting from the beginning of July 2019. The results show that the weight of the valuation criteria is higher in the order of farm acceptability, feasibility, and policy efficiency. Compliance with PLS standards, compliance with disposal standards of waste vinyl and pesticides, soil testing, compliance with toxic substance standards, education, etc. are comparatively evaluated to be higher cross-compliance items in basic cross-compliance. Disposing of an abandoned well, jointly collecting and disposing of agricultural by-products, common area care and cleaning, maintenance of empty houses and poor facilities, growing green manure crops during the fallow period, etc. are comparatively evaluated to be higher cross-compliance items for the additional cross-compliance. The results of this study are expected to contribute to the government's policy related to the cross-compliance of public-benefit direct payment.

• Analytical Science and Technology
• /
• v.15 no.4
• /
• pp.381-387
• /
• 2002

In this study, we introduced experience participated in ELPAT (Environmental Lead for Proficiency Analytical Testing) program that is administered by the American Industrial Hygiene Association and EPA. The 126 sample results of total 128 samples met reference value, as a result accepted 'proficient'. The concentration of Pb in air, paint chips, soils and dust wipes are $0.0089{\sim}0.3956mg/m^3$, 0.0500~8.9149%, 29.0${\sim}$1697 mg/kg and $18.00{\sim}900.3{\mu}g/sample$, respectively. The lead in environmental matrix prepared adequate techniques and analysed by flame atomic absorption spectrometry. The lead in air was extracted hydrochloric acid, in paint chips and soils were digested with microwave and hotplate and in dust wipes were prepared microwave digestion. No differences of each preparation techniques was found and all methods were to be proper.

• Research in Plant Disease
• /
• v.22 no.3
• /
• pp.152-157
• /
• 2016

Root-dipping inoculation method has been used to investigate resistance of radish plants to Fusarium wilt. However, the method requires a lot of labor and time because of complicate procedure. This study was conducted to establish a simple and effective mass-screening method for resistant radish to Fusarium wilt. Radish seedlings of susceptible and resistant cultivars were used to investigate wounding method by scalpel, inoculum concentration, and pathogen-inoculated growth stage of seedlings. We established an efficient mass-screening method based on our results as following: Roots of 14-day-old seedlings of radish are cut with a scalpel at a $90^{\circ}$ angle to a 2 cm-depth at a 1 cm-distance from main stem and then inoculated by pouring with a 10 ml-aliquot of a fungal spore suspension ($1.0{\times}10^7conidia/ml$) on soil. The inoculated plants are cultivated in a growth room at $25^{\circ}C$ for about 4 weeks with 12-hour light a day. The proposed screening method enables to effectively select resistant from mass radish plants cultivars to Fusarium wilt.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.6 no.4
• /
• pp.245-255
• /
• 2008

Korea Hydro and Nuclear Power Company(KHNP) conducted site investigations for a low and intermediate-level nuclear waste repository in the Gyeong Ju site. The site characterization work constitutes a description of the site, its regional setting and the current state of the geosphere and biosphere. The main objectives of hydogeological investigation aimed to understand the hydrogeological setting and conditions of the site, and to provide the input parameters for safety evaluation. The hydogeological characterization of the site was performed from the results of surface based investigations, i.e geological mapping and analysis, drilling works and hydraulic testing, and geophysical survey and interpretation. The hydro-structural model based on the hydrogeological characterization consists of one-Hydraulic Soil Domain, three-Hydraulic Rock Domains and five-Hydraulic Conductor Domains. The hydrogeological framework and the hydraulic values provided for each hydraulic unit over a relevant scale were used as the baseline for the conceptualization and interpretation of flow modeling. The current hydrogeological characteristics based on the surface based investigation include some uncertainties resulted from the basic assumption of investigation methods and field data. Therefore, the reassessment of hydrostructure model and hydraulic properties based on the field data obtained during the construction is necessitated for a final hydrogeological characterization.

• Journal of Food Hygiene and Safety
• /
• v.32 no.4
• /
• pp.306-313
• /
• 2017

This study systemically analyzed the data on the microbial levels in fresh vegetables in Korea to identify the points to control. We scanned the studies published between 2001 and 2015 in peer-reviewed research papers on the microbial levels in fresh vegetables produced in Korea. Plant products were categorized by using the US IFSAC (Interagency Food Safety Analytics Collaboration) category. The most consumed, the non-heat treated, the epidemiological foodborne diseases sources of fresh vegetable in foodservice (KCDC data) were identified by literature review. Articles were screened using National Digital Science Library (NDSL) search engine regarding to microbial hazards in plant products. Based on the total plate count number and coliforms on the 89 data cases from 26 published articles, the total plate count number was high in the order of sprouts, leaf and stem, bulbs and roots, vine-grown, solanaceous, melons, and pome. Escherichia coli was frequently detected in leaf and stem and sprouts products. Focused on the microbial data of leek, lettuce and cabbage, the levels of total plate count, coliforms and Bacillus cereus showed the levels of 4.15~7.69 log CFU/g, 1~6.99 log CFU/g, and 0.51~3.9 log CFU/g, respectively, by 33 published papers. The levels of environmental factors affecting the microbial safety of lettuce and leek before harvest were investigated. Manure, soil, hands, scale, gloves were the major potential microbial contamination points to control. In addition, GAP (good agricultural practice), microbial testing, and improvement of irrigation methods are required to provide the safer fresh produce.