• Journal of Microbiology and Biotechnology
• /
• v.16 no.2
• /
• pp.193-199
• /
• 2006

Halotolerant spore-forming Gram-positive bacteria were isolated from the root, rhizosphere, and non-rhizosphere soil of Blutaparon portulacoides. The different isolates were characterized genetically using an amplified ribosomal DNA restriction analysis (ARDRA), and phenotypically based on their colonial morphology, physiology, and nutritional requirements. Three different 16S rRNA gene-based genotypes were observed at a 100% similarity using the enzymes HinfI, MspI, and RsaI, and the phenotypic results also followed the ARDRA groupings. Selected strains, representing the different ARDRA groups, were analyzed by 16S rDNA sequencing, and members of the genera Halobaeillus, Virgibacillus, and Oceanobacillus were found. Two isolates showed low 16S rDNA sequence similarities with the closest related species of Halobacillus, indicating the presence of new species among the isolates. The majority of the strains isolated in this study seemed to belong to the species O. iheyensis and were compared using an AP-PCR to determine whether they had a clonal origin or not. Different patterns allowed the grouping of the strains according to Pearson's coefficient, and the resulting dendrogram revealed the formation of two main clusters, denoted as A and B. All the strains isolated from the soil were grouped into cluster A, whereas cluster B was exclusively composed of the strains associated with the B. portulacoides roots. This is the first report on the isolation and characterization of halotolerant spore-forming Gram-positive bacteria that coexist with B. portulacoides. As such, these new strains may be a potential source for the discovery of bioactive compounds with industrial value.

• Journal of Environmental Impact Assessment
• /
• v.13 no.3
• /
• pp.137-151
• /
• 2004

Many of today's environmental problems are regional in scope and their effects overlap and interact. The purpose of this paper is to developed a simple method for an integrated assessment of environmental conditions across the Mid-Nakdong River Region, by combining data on land use, impervious cover, roads, streams, riparian areas, forest patches, population, pollutant loadings, soil erosion and topography. A cluster analysis was used to identify groups of sub-watersheds with similar environmental characteristics. The mean value for each group was used to find watershed that may be more vulnerable to future environmental degradation. Watersheds in cluster I and II had high amount of forest, but the amount of riparian vegetation was low. Watersheds in cluster III, which located in the middle Geumho River and the main course of Nakdong River, had a greater proportion of their agriculture, a greater proportion of agriculture on steep slopes, and less forest adjacent to streams. Watersheds in cluster IV and V were in the most urbanized areas of the region. The principal adverse impacts for watersheds in this group were high scores for urban area, impervious cover, pollutant loadings, population density, forest fragmentation, and low amounts of forest and riparian forest cover. Notwithstanding the exploratory nature of cluster analysis, it appears to be a useful tool for grouping watersheds with similar environmental characteristics.

• The Plant Pathology Journal
• /
• v.36 no.1
• /
• pp.29-42
• /
• 2020

Acidovorax citrulli causes bacterial fruit blotch in Cucurbitaceae, including watermelon. Although A. citrulli is a seed-borne pathogen, it can cause diverse symptoms in other plant organs like leaves, stems and fruits. To determine the infection routes of A. citrulli, we examined the virulence of six isolates (Ac0, Ac1, Ac2, Ac4, Ac8, and Ac11) on watermelon using several inoculation methods. Among six isolates, DNA polymorphism reveals that three isolates Ac0, Ac1, and Ac4 belong to Clonal Complex (CC) group II and the others do CC group I. Ac0, Ac4, and Ac8 isolates efficiently infected seeds during germination in soil, and Ac0 and Ac4 also infected the roots of watermelon seedlings wounded prior to inoculation. Infection through leaves was successful only by three isolates belonging to CC group II, and two of these also infected the mature watermelon fruits. Ac2 did not cause the disease in all assays. Interestingly, three putative type III effectors (Aave_2166, Aave_2708, and Aave_3062) with intact forms were only found in CC group II. Overall, our results indicate that A. citrulli can infect watermelons through diverse routes, and the CC grouping of A. citrulli was only correlated with virulence in leaf infection assays.

• Korean Journal of Soil Science and Fertilizer
• /
• v.19 no.3
• /
• pp.211-216
• /
• 1986

To establish a suitability grouping system of paddy soils for double or multiple cropping with rice which is intensively practiced in the southern parts of Korea, a few basic experiments were carried out for two years. The results are summarized as follows; 1. The potential productivities of the paddy soils which were tested without any fertilizer in the pots of subsoil samples by the double cropping of rice and other upland crops were resulted that the soils of "Moderately well drained" fine silty textured were the highest while the soils of "Poorly drained" sandy were the lowest, and the productivities could be clearly comparable according to the differences of soil conditions. 2. The decomposability of organic matter also was higher in the soils of "Moderately well drained" than the "Imperfectly drained". The coarse loamy and coarse silty textured soils were high in the upland condition and in the early stages of submerging while the fine loamy and fine silty textured soils were high at the late stage of submerging in the rates of organic matter decomposition. 3. The days to be reached to tillable condition after rainfall in fine loamy textured soils were about 5 days earlier than the clayey soils. The period of tillable condition of fine clayey soils with "Moderately well drained" was the longest and that of the fine loamy textured soils was the shortest. But the soils with "Imperfectly drained" were not clear among soil textural classes. 4. The lower the ground water table the higher was the productivity indices. The variation of ground water table in the medium textured soils was higher than the both of coarse and fine textured soils among "Moderately well drained". But it was observed the opposite in the soils of "Imperfectly drained".

• Journal of the Korean Geotechnical Society
• /
• v.23 no.12
• /
• pp.33-42
• /
• 2007

The assessment of shear wave velocity($V_s$) in soft soils is extremely difficult due to the soil disturbances during sampling and field access. After a ring type field $V_s$ probe(FVP) has been developed, it has been applied at the southern coastal area of the Korean peninsular. This study presents the upgraded FVP "blade type FVP", which minimizes soil disturbance during penetration. Design concerns of the blade type FVP include the tip shape, soil disturbance, transducers, protection of the cables, and the electromagnetic coupling between transducers and cables. The cross-talking between cables is removed by grouping and extra grounding of the cables. The shear wave velocity of the FVP is simply calculated by using the travel distance and the first arrival time. The large calibration chamber tests are carried out to investigate the disturbance effect due to the penetration of FVP blade and the validity of the shear waves measured by the FVP. The blade type FVP is tested in soils up to 30m in depth. The shear wave velocity is measured every 10cm. This study suggests that the upgraded blade type FVP may be an effective device for measuring the shear wave velocity with minimized soil disturbance in the field.

• Korean Journal of Soil Science and Fertilizer
• /
• v.17 no.4
• /
• pp.330-336
• /
• 1984

These studies were aimed to find out the representative values of physico-chemical properties in Korean soils and to serve the basic informations to improve the soil properties for increasing the soil productivity. By the results of detailed soil survey, 14 physico-chemical properties (soil texture, moisture characteristics, organic matter content etc) were collected from 315 series of soils except Cheju soils, computerized the simple mean and the distributed area by weighted mean values with grouping of land use, drainage class and soil type. The results were as follows; 1. Simple mean values within the efficient soil depth in whole country soils were clay 20.0%, organic matter 2.03% and CEC 10.3me/100g. But weighted mean values with land area belonged to the same soil series were clay 18.0%, OM 1.85% and CEC 8.6me/100g. 2. Mean values of paddy soils weighted by area were clay 19.7%, OM 2.0% and CEC 9.1me/100g but those of upland soils were 17.9%, 1.8% and 8.1me/100g and forest soils were 16.7%, 1.8% and 8.6me/100g respectively. 3. Weighted mean values of moderately well soils with covered area were clay 18.9%, organic matter 1.7%, CEC 8.4me/100g but those of imperfectly drained soils were 21.3%, 2.2% and 9.5me/100g, and those of poorly drained soils were 15.1%, 1.8% and 9.9me/100g respectivcly. 4. Simple mean and area weighted mean values of clay content, pH, organic matter contents, cation exchange capacity and base saturation were calculated by the types of paddy and upland.

• Journal of Korean Society of Forest Science
• /
• v.90 no.4
• /
• pp.535-542
• /
• 2001

The objective of this study is to develop a spatial analysis modeling technique to evaluate the functional suitability of forest lands for land slide prevention. The functional suitability is classified into 3 categories of high, medium and low according to the potential of land slide on forest lands. The potential of land slide hazards is estimated using the measurements of 7 major site factors : slope, bed rock, soil depth, shape of slope, forest type and D.B.H. class of trees. The analytic hierarchical process is applied to determining the relative weight of site factors in estimating the potential of land slides. The spatial analysis modeling starts building base layers for the 7 major site factors by $25m{\times}25m$ grid analysis or TIN analysis, reclassifies them and produces new layers containing standardized attribute values, needed in estimating land slide potential. To these attributes, applied is the weight for the corresponding site factor to build the suitability classification map by map algebra analysis. Then, finally, cell-grouping operations convert the suitability classification map to the land unit function map. The whole procedures of the spatial analysis modeling are presented in this paper.

• Korean Journal of Environmental Biology
• /
• v.17 no.3
• /
• pp.321-330
• /
• 1999

Nematodes were isolated using silkwom trap through the investigation of 100 soil samples from various biotopes in Korea. The 30 nematode strains from soil and dead insects by the pathogenicity aganinst silkworms (Bombyx mori mori) and insect pests of Calliphora vomitoria, Pseufazetia separata, Palomena angulosa, and Melolontha incana. Mortailty of the silkworm larvae and pupae were as high as 100% by nematode infection, those of insect of pests were varied from 20 to 100%. The 30 strains of entemopathogenic nematodes were classified into five groups of Rhabditidae, Diplogatroidae, Heterorhabitidae, Steinernematidae, and Tylenchida by morphological criteria. The genetic relationships among the 30 nematode strains were analyzed by various RAPD bands with twenty primers. The 30 nematode strains were classified into six major subgroups on the basis of the genetic similarity coefficient of 0.853. The grouping by RAPD was agree with those of morphological taxa in discrimination of the higher group, however, was not completely agree in the subgroup. The family Steinernematidae belong to Rhabditida was clarified as closer to the Tylenchida, rather than the other Rhabditida of Heterorhabitidae, Rhabditidae, and Diplogatroidae in genetic distance valule. From the result of the morphological classification and RAPD of the genomic DNA showed that genetic relationship analysis furnish infurmation on phylogenetic classification and relationships of entomopathogenic nematodes. The application of genetic similarity will overcome the limitation of taxonomy and classification of morphologically simple nematode. Several primers were confirmed those utility of identification for individual nematode strains, the methods of molecular genetics secured the simplicity, rapidity and accuracy on the selection of entomopathogenic nematodes.

• Korean Journal of Plant Resources
• /
• v.37 no.2
• /
• pp.214-224
• /
• 2024

In this study, the growth, physical characteristics of fruit and soil chemistry were investigated by grouping S. thea populations. The soil for each group was sandy loam or sandy loam, and it was analyzed to be slightly acidic to neutral, with a pH ranging from 5.6 to 7.0. Additionally, the leaf size was highest in the G7 group, and overall fruit growth was highest in the G1 group, confirming differences between the groups. The sweetness ranged from 16.8° Brix to 12.3° Brix, indicating a higher sweetness compared to Vaccinium oldhamill, blueberry, and blackberry. The correlation analysis between soil chemistry and S. thea growth characteristics revealed a significant negative correlation between calcium (Ca) and fruit growth characteristics. Furthermore, a significant positive correlation was observed between sodium (Na) and fruit acidity, as well as between cation exchange capacity (CEC) and fruit hardness.

• The Korean Journal of Mycology
• /
• v.26 no.3 s.86
• /
• pp.373-379
• /
• 1998

Rhizoctonia solani [Thanatephorus cucumeris (frank) Donk], one of the major soil-borne plant pathogens with world-wide distribution, can cause great damages on various crops. In Korea, sheath blight on rice caused by this pathogen is the major concern, and active studies on this pathogen have been performed. However, most of these studies were concerned with pathogenicity of the isolates instead of molecular analyses of different AGs of R. solani. Therefore, in this study, thirty isolates of Rhizoctonia solani collected from various sources were used for the analyses of genetic relationships among themselves and for the rapid anastomosis grouping with RAPD method. As a result, thirty isolates of known and unknown AGs were grouped into five subgroups and each group included AG-1, AG-2, AG-3, AG-4, and AG-5. RS-1 isolate was found to be closely related to AG-5. Isolates RS-4, RS-14, RS-17, and RS-16 were found to be closely related to AG-2-2(III B). Isolate RS-13 was closely related to AG-4, isolates RS-8 and RS-10 were closely related to AG-1(I B), and isolates RS-7 and RS-21 were closely related to AG-2-2(IV). Isolate RS-19 was closely related to AG-1(I C), and isolates RS-3, RS-5, RS-18, RS-6, and RS-15 were found to be closely related to AG-1.