The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Analyses of Genetic Relationships of Rhizoctonia solani Isolates from Various Crop Species and Rapid Identification of Anastomosis Groups with RAPD Method

각종 작물에서 분리한 R. solani 균주들의 RAPD를 이용한 종내 그룹의 유전적 유연관계 분석 및 AGs 신속 간이동정

  • Lee, Youn-Su (Div. of Applied Plant Sciences, Kangwon National University) ;
  • Choi, Hei-Sun (Div. of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Kyoung-Su (Div. of Applied Plant Sciences, Kangwon National University) ;
  • Woo, Su-Jin (Div. of Applied Plant Sciences, Kangwon National University) ;
  • Kang, Won-Hee (Institute of Agricultural Science, Kangwon National University) ;
  • Kim, Myoung-Jo (Institute of Agricultural Science, Kangwon National University) ;
  • Shim, Jae-Ouk (Department of Applied Biology, Dongguk University) ;
  • Lee, Min-Woong (Department of Applied Biology, Dongguk University)
  • 이윤수 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 최혜선 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 김경수 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 우수진 (강원대학교 농업생명과학대학 식물응용과학부) ;
  • 강원희 (강원대 농업과학연구소) ;
  • 김명조 (강원대 농업과학연구소) ;
  • 심재욱 (동국대학교 응용생물학과) ;
  • 이민웅 (동국대학교 응용생물학과)
  • Published : 1998.09.30
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Abstract

Rhizoctonia solani [Thanatephorus cucumeris (frank) Donk], one of the major soil-borne plant pathogens with world-wide distribution, can cause great damages on various crops. In Korea, sheath blight on rice caused by this pathogen is the major concern, and active studies on this pathogen have been performed. However, most of these studies were concerned with pathogenicity of the isolates instead of molecular analyses of different AGs of R. solani. Therefore, in this study, thirty isolates of Rhizoctonia solani collected from various sources were used for the analyses of genetic relationships among themselves and for the rapid anastomosis grouping with RAPD method. As a result, thirty isolates of known and unknown AGs were grouped into five subgroups and each group included AG-1, AG-2, AG-3, AG-4, and AG-5. RS-1 isolate was found to be closely related to AG-5. Isolates RS-4, RS-14, RS-17, and RS-16 were found to be closely related to AG-2-2(III B). Isolate RS-13 was closely related to AG-4, isolates RS-8 and RS-10 were closely related to AG-1(I B), and isolates RS-7 and RS-21 were closely related to AG-2-2(IV). Isolate RS-19 was closely related to AG-1(I C), and isolates RS-3, RS-5, RS-18, RS-6, and RS-15 were found to be closely related to AG-1.

Rhizoctonia solani의 종내 그룹의 분류에는 균사융합군과 배양형태가 많이 이용되고 있으며, 이미 20종 이상의 R. solani가 생태학적, 형태학적, 효소학적, hyphal anastomosis 등에 의해 이미 구분되어졌다. Anastomosis group은 R. solani를 분리하는데 유용하지만 R. solani의 생물학적과 병리학적 연구를 위한 유전적 특성과 동정의 직접적인 방법이 요구되어진다. RAPD는 특별한 DNA 절편을 증폭하고 이를 genetic mapping, identification of isolates에 유용하게 적용될 수 있으며, 또한 genetic variation 조사에도 사용될 수 있다. Dendrogram을 작성한 결과 크게 5 group으로 나뉘어졌고, 5개의 group은 AG group의 subgroup과 동일하게 나뉘어졌다. AG group에 구분되지 않은 species들도 RAPD결과 AG tester들과 grouping 되어졌다. RS-1은 AG-5 group에 속하며, RS-4, RS-14, RS-17, RS-16은 AG-2-2(III B)에 속하였다. RS-13은 AG-4에 속하였으며, RS-8과 RS-10은 AG-1(I B)에, RS-7과 RS-21은 AG-2-2(IV)에 속하였다. RS-19는 AG-1-1(I C)에 속하고, RS-3, RS-5, RS-18, RS-6, RS-15는 AG-1에 속하였다. RAPD 결과 AG group의 subgroup간의 차이를 볼 수 있었고, 이에 AG group 되어 있지 않은 species의 AG grouping이 이루어졌다. 또한 subgroup 간의 유전적 차이를 확인 할 수 있는 marker를 개발하거나 subgroup의 특별한 primer를 제작하는 SCAs기법을 이용하여 식물체 병반 또는 토양에서 분리된 R. solani를 간이 동정에 이용할 수 있을 것으로 기대한다.

Keywords

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