• Journal of Microbiology
• /
• v.34 no.3
• /
• pp.229-235
• /
• 1996

Microgram quantities of DNA per gram soil were recovered with SDS- based and freeze-and thaw procedures. The average DNA fragment size was > 23 Kb. This method generated minimal shearing of extracted DNA. However, the DNA extracts still contained considerable amounts of humic impurities sufficient to inhibit PCR. Several approaches were used to reduce the interferences with the PCR (use of CTAF in extraction step, Elutip-d column purification, addition of BSA to PCR buffer) to accomplish PCR with DNA extract as a template. Most of the DNA extracts were not digested completely by restriction endonuclease, and CTAB-TREATED ane Elutip-d column purified DNA extracts were partially digested. Regarding as restriction enzyme digestion, all PCRs failed to amplify 16S rRNA gene fragments in the DNA extracts. In the case of DNA extracts only where BSA was added to PCR buffer, PCR was successfully conducted whether the DNA extracts were treated with CTAB or purified with columns. However, these two treatments were indispensable for humic impurity-rich DNA extracts to generate the PCR-compatible DNA samples. Direct extraction of DNA, coupled with these procedures to remove and relieve interferences by humic impurities and followed by the PCR, can be rapid and simple method for molecular microbiological study on soil microorganisms.

• Proceedings of the Korean Radioactive Waste Society Conference
• /
• 2003.11a
• /
• pp.696-700
• /
• 2003

$H_2SO_4$ and citric acid had higher extraction efficiency of $^{137}Cs$ from soil than the other chemicals. Thus, $H_2SO_4$and citric acid were used as additives on remediation experiment by electrokinetic method to increase removal efficiency of $^{137}Cs$ from the radioactive soil being stored during a long time. An average velocity of effluent discharged from experimental column $2.0{\times}10^{-2}$/cm/min and a volume of the discharged soil wastewater for 10 days is 3.6 Pore Volume. The 54% of a total of $^{137}Cs$ in the column was decontaminated for 10 days. Furthermore, the predicted values of residual concentration by the developed model were quite similar to those obtained from experiments.

• Journal of the Korean GEO-environmental Society
• /
• v.5 no.1
• /
• pp.35-46
• /
• 2004

In Korea, around one - third of the country is occupied by granite, and granite weathered soils are widely distributed. Most of the research on this soil has been performed using reconstituted specimens because of the extreme difficulty of undisturbed sampling due to the sensitive particle structures. Therefore, the comparisons of deformational characteristics, which is expressed in terms of shear and Young's moduli and damping ratio, obtained from the undisturbed and reconstituted specimens are important for the reliable understanding of soil behavior. In this study, the resonant column and torsional shear tests were performed on granite weathered soils in Korea, and the deformation characteristics of undisturbed and reconstituted soil on granite weathered soils were evaluated and compared.

• Steel and Composite Structures
• /
• v.30 no.2
• /
• pp.97-108
• /
• 2019

This paper presents a new model for the strap combined footings to obtain the most economical contact surface on the soil (optimal dimensioning) to support an axial load and moment in two directions to each column. The new model considers the soil real pressure, i.e., the pressure varies linearly. Research presented in this paper shows that can be applied to the T-shaped combined footings and the rectangular combined footings. The classical model uses the technique of test and error, i.e., a dimension is proposed, and subsequently, the equation of the biaxial bending is used to obtain the stresses acting on each vertex of the strap combined footing, which must meet the conditions following: The minimum stress should be equal or greater than zero, and maximum stress must be equal or less than the allowable capacity that can withstand the soil. Numerical examples are presented to obtain the optimal area of the contact surface on the soil for the strap combined footings subjected to an axial load and moments in two directions applied to each column. Appendix shows the Tables 4 and 5 for the strap combined footings, the Table 6 for the T-shaped combined footings, and the Table 7 for the rectangular combined footings.

• Journal of the Korean Geotechnical Society
• /
• v.33 no.3
• /
• pp.37-47
• /
• 2017

The geotechnical characteristics of frozen ground is one of the key design issues for the construction of infrastructure in cold region. In this study, the dynamic properties (shear modulus and damping ratio) of frozen and unfrozen soils sampled from Terra Nova Bay located in eastern Antarctica, where Jang Bogo station was built, were investigated using Stokoe-type resonant column test (RC). In order to freeze the reconstituted soil specimen, the RC testing equipment was modified by adding a cooling system. A series of resonant column tests were performed in frozen and unfrozen soils with various soil densities and temperatures. The shear modulus (G) and damping ratio (D) of soil frozen at $-7^{\circ}C$ were compared with those of unfrozen soil. In addition, the effect of temperature rise on the maximum shear modulus ($G_{max}$) and damping ratio was experimentally investigated. This study has significance in that the difference of dynamic soil properties between frozen and unfrozen soils and the effect of temperature rise on frozen soil were identified.

• Korean Journal of Environmental Agriculture
• /
• v.33 no.3
• /
• pp.164-168
• /
• 2014

BACKGROUND: Increase in application of liquid pig manure(LPM) in agriculture as nutritional source has become a social issue due to its influence on water quality. Also, proper application methods have not been developed with respect to indigenous properties of LPM and soil physical properties. Therefore, we conducted this experiment to observe the infiltration characteristics and distribution of dissolved organic compounds of LPM in soils having different soil textures. METHODS AND RESULTS: To do this experiment, we collected three different soils and LPM. We analyzed the physical and chemical properties of both soils and LPM to determine the dilution ratios of LPM. The LPM diluted to 4 different ratios with distilled water was applied to the top of soil column. Infiltration rates were observed by time and depth until the amount of effluent collected from the bottom of the soil columns were stabilized while maintaining the hydraulic head 3 cm above the soil column. The results showed that infiltration rates increased with increasing dilution ratios in the order of sandy, loamy sand, and sandy loam. The time required to reach steady state was increased with decreasing sand contents clay. CONCLUSION: The size and amount of the dissolved organic compounds in LPM that can determine the efficiency as fertilizer and environmental problems as nonpoint pollution source in water quality have not been investigated with respect to behavior and transport of them in soil. Therefore, it requires further research how we can properly apply LPM as valuable fertilizer substitute for inorganic fertilizers.

• Journal of Soil and Groundwater Environment
• /
• v.7 no.4
• /
• pp.87-91
• /
• 2002

Lab scale batch and column tests were performed to investigate the treatability of petroleum contaminated soil using the in-situ soil flushing method. The pyrex column (4.5$\times$25 cm) was used to investigate optimal washing agent, surfactant concentration, mixing ratio, and inlet velocity. The miked surfactant of $POE_{14}$ and SDS were determined as ideal systems for the batch tests. From the results of preliminary tests, mixed surfactant was found to be more harmful for microorganisms. So $POE_{5}$ and $POE_{14}$ were chosen as the surfactant system for the batch study. The washing efficiency for the diesel contaminated soil was increased until 1 %, and decreased after l %. When applied as selected mixed surfactant, the ideal mixed ratio was recognized as 1:1. Therefore we selected miked surfactant $POE_{5}$ and $POE_{14}$, surfactant concentration 1%, and mixed ratio 1:1 for the remediation of diesel contaminated soil. In column tests, the total removal efficiency was improved as the flux of washing agent was increased. At the same pore volume, small flux showed better removal efficiency.

• Geomechanics and Engineering
• /
• v.7 no.5
• /
• pp.539-552
• /
• 2014

A simplified probability-based design charts for stone column-improved ground have been presented based on the unit cell approach. The undrained cohesion ($c_u$) and coefficient of radial consolidation ($c_r$) of the soft soil are taken as the most predominant random variables. The design charts are developed to estimate the diameter of the stone column or the spacing between the stone columns by employing a factored design value of $c_r$ and $c_u$ so as to satisfy a specific probability level of the target degree of consolidation and/or a target safe load that needs to be achieved in a specified timeframe. The design charts can be used by the practicing engineers to design the stone column-improved ground by considering consolidation and /or bearing capacity of the improved ground.

• Journal of the Korean Geosynthetics Society
• /
• v.20 no.2
• /
• pp.13-22
• /
• 2021

Pile or column supported embankments have been increasingly employed to construct highway or railway embankments over soft soils. Piles or columns of stiffer material installed in the soft ground can provide the necessary support by transferring the embankment load to a firm stratum using a soil arching. However, there has been reported to occur a relatively large differential settlement between the piles and the untreated soils. Geosynthetic reinforced pile or column supported embankment (GRPS) is often used to minimize the differential settlement. Two dimensional finite element anlyses have been performed on both the column supported embankments and the geogrid reinforced column supported embankments by using a PLAXIS 2D to evaluate the soil arching effect. Based on the results obtained from finite element analyses, the stress reduction ratio decreases as the area replacement ratio increases in the column supported embankments. For the geogrid reinforced column supported embankments, the geogrid reinforcemnt can reduce differential settlements effectively. In additon, the use of stiffer geogrid is appeared to be more effective in reducing the differential settlements.

• Journal of Soil and Groundwater Environment
• /
• v.16 no.5
• /
• pp.42-52
• /
• 2011

In this laboratory study, the changes in gas-exposed perchloroethene (PCE) saturation in sand during a PCE removal process were measured using gaseous tracers. The flux of fresh air through a glass column packed with PCEcontaminated, partially water-saturated sand drove the removal of PCE from the column. During the removal of PCE, methane, n-pentane, difluoromethane and chloroform were used as the non-reactive, PCE-partitioning, water-partitioning, and PCE and water-partitioning tracers, respectively. N-pentane was used to detect the PCE fraction exposed to the mobile gas. At water saturation of 0.11, only 65% of the PCE was found to be exposed to the mobile gas prior to the removal of PCE, as calculated from the n-pentane retardation factor. More PCE than that detected by n-pentane was depleted from the column due to volatilization through the aqueous phase. However, the ratio of gas-exposed to total PCE decreased on the removal of PCE, implying gas-exposed PCE was preferentially removed by vaporization. These results suggest that the water-insoluble, PCE-partitioning tracer (n-pentane in this study), along with other tracers, can be used to investigate the changes in fluid (including nonaqueous phase liquid) saturation and the removal mechanism during the remediation process.