• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2002년도 춘계 국제 학술대회
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• pp.9-12
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• 2002

Diffusive transport of volatile organic compounds(VOCs) and their degradation by bacteria in unsaturated soils are couple by poorly understood mass transfer kinetics at the gas/water interface. Determination of the fate of VOCs in unsaturated soil is necessary to evaluate the feasibility of natural attenuation as a VOC remediation strategy. The objective of this study was to develop a mechanistically based mathematical model that would consider the interdependence of VOC transport, microbial activity, and sorptive interaction in a moist, unsaturated soil. Because the focus of the model was on description of natural attenuation, the advective VOC transport that is induced in engineered remediation processes such as vapor extraction was not considered. The utility of the model was assessed through its ability to describe experimental observations form diffusion experiments using toluene as a representative VOC in well-defined soil columns that contained a toluene degrading bacterium, Pseudomonas Putida, as the sole active microbial species. The coefficient for gas-liquid mass-transfer, K$\sub$LA/, was found to be a key parameter controlling the ability of bacteria to degrade VOCs. This finding indicates that soil size and geometry are likely to be important parameters in assessing the possible success of natural attenuation of VOCs in contaminated unsaturated soils.

• 환경위생공학
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• 제19권4호
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• pp.19-24
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• 2004

The objective of this study was to develop a mechanistically based mathematical model that would consider the interdependence of VOCs transport, microbial activity, and sorptive interactions in a moist, unsaturated soil. Because the focus of the model was on description of natural attenuation, the advective VOCs transport that is induced in engineered remediation processes such as vapor extraction was not considered. The utility of the model was assessed through its ability to describe experimental observations from diffusion experiments using toluene as a representative VOCs in well-defined soil columns that contained a toluene degrading bacterium, Pseudomonas putida G7 md Fl, as the sole active microbial species. The gas-liquid mass-transfer was found to be a key parameter controlling the ability of bacteria to degrade VOCs. This finding indicates that soil size and geometry are likely to be important parameters in assessing the possible success of natural attenuation of VOCs in contaminated unsaturated soils. Therefore we found that Pseudomonas putida G7 and Fl were very effective to remove of refractory pollutants such as toluene in soil by Bio-filter

• 한국미생물·생명공학회지
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• 제44권2호
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• pp.185-193
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• 2016

경상북도 경산시에 위치한 영남대학교 내의 토양으로부터 protease를 생산하는 신규 미생물 FBL-1을 분리하였다. 본 균주는 Biolog test 및 API 50CHB test 결과, Bacillus 속 미생물 중 하나인 것으로 확인되었다. 16S rDNA 염기서열 분석결과로부터 FBL-1 균주는 B. subtilis subsp. subtilis NCIB 3610 (99.5%), B. subtilis subsp. inaquosorum KCTC 13429 (99.4%), B. subtilis subsp. spizizenii NRRL B-23049 (99.3%), B. methylotrophicus KACC 13105 (99.3%) 등과 높은 상동성을 보였다. 이러한 생리적, 형태학적, 계통학적 특성에 따라 균주 FBL-1은 B. subtilis에 속하는 균으로 최종 동정하여 B. subtilis FBL-1으로 명명하였다. B. subtilis FBL-1을 이용한 protease 생산시 탄소원으로는 fructose, 질소원으로는 yeast extract가 균체 성장 및 protease 활성을 위하여 가장 적합한 것으로 나타났다. B. subtilis FBL-1 균주는 protease를 생산하는데 활용할 수 있으며, 향후 배지 및 발효조건 최적화와 효소의 특성 규명 등의 연구를 통하여 식품, 세제 등의 다양한 산업에 유용하게 사용될 수 있을 것으로 기대된다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.218.2-219
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• 2003

2-Hydroxymuconic semialdehyde dehydrogenase catalyzes the conversion of 2-hydroxymuconic semialdehyde (HMS) to an enol form of 4-oxalocrotonate which is a step in the catechol-meta cleavage pathway. A tomC gene encoding 2-HMS dehydrogenase of Burkholderia cepacia G4, a soil bacterium that can grow on toluene, cresol, phenol or tricholoro ethylene, is identified in between catechol 2,3-dioxygenase gene and HMS hydrolase gene, its sequence is analysed and the enzyme is characterised. (omitted)

• 미생물학회지
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• 제11권2호
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• pp.59-62
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• 1973

Bacterium strain, K-173-10, which was isolated from waste soil of Korean brewing factories, could grow on acetate as the sole carbone source and accumulate a considerable amount of L-glutamic acid (24g/l) in the liguid culture medium. This strain was named by Brevibacterium ammoniagenes sp. by the standard method of taxonomy procedures given in the Manual of Microbiogical Methods.

• 미생물과산업
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• 제26권1호
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• pp.2-7
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• 2000

1. 양조폐수장, 폐수구의 토양에서 분리한 K-173-10은 초산을 자화하여 L굴루타민산을 24g/l생성하였다. 2. 분리된 균주는 행태학적, 생리학적, 분류학적 특성으로 미루어 보면 Brevibacterium ammoniagenes sp.로 간주된다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.108.3-109
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• 2000

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• 제22권5호
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• pp.674-683
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• 2012

A new strain, SD12, was isolated from tannery waste polluted soil and identified as Pseudomonas aeruginosa on the basis of phenotypic traits and by comparison of 16S rRNA sequences. This bacterium exhibited broad-spectrum antagonistic activity against phytopathogenic fungi. The strain produced phosphatases, cellulases, proteases, pectinases, and HCN and also retained its ability to produce hydroxamate-type siderophore. A bioactive metabolite was isolated from P. aeruginosa SD12 and was characterized as 1-hydroxyphenazine ((1-OH-PHZ) by nuclear magnetic resonance (NMR) spectral analysis. The strain was used as a biocontrol agent against root rot and wilt disease of pyrethrum caused by Rhizoctonia solani. The stain is also reported to increase the growth and biomass of Plantago ovata. The purified compound, 1-hydroxyphenazine, also showed broad-spectrum antagonistic activity towards a range of phytopathogenic fungi, which is the first report of its kind.

• 한국지하수토양환경학회지:지하수토양환경
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• 제21권1호
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• pp.40-48
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• 2016

A siderophore-producing bacterium (i.e., Pseudomonas aeruginosa) capable of chelating Fe³⁺ from its mineral form (i.e., iron oxides) was used to enhance As uptake by plants. Since As in soil is mainly associated with iron oxides, siderophore can play an important role in As mobilization through the dissolution of As-bearing iron oxides. A series of pot experiment using Pteris cretica showed that As removal by P. cretica with siderophore-producing bacteria addition increased more than three times compared to that without bacteria addition. Competition between indigenous bacteria and introduced bacteria (i.e., P. aeruginosa) was also observed, but such competition seemed not to be significant. This study suggests that enhanced-phytoremediation by siderophore-producing bacteria addition could be a visible option for longterm As removal in the forest area at the former Janghang smelter site.

• 한국환경농학회지
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• 제32권2호
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• pp.148-154
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• 2013

본 연구는 CVX 바이러스저항성 삼각주 재배가 토양 미생물에 미치는 영향과 수평적 유전자 이동성을 확인하기 위해 수행되었다. 생육시기별 토양 세균과 방선균 군집밀도는 형질전환 삼각주 재배 토양의 미생물 군집밀도와 비형질전환 삼각주 군집밀도가 유사하여 토양 미생물에 미치는 영향은 유사할 것으로 추정되었다. 토양 미생물의 우점종은 Proteobacteria, Uncultured archaeon와 Uncultured bacterium으로 나타났으며, 형질전환 삼각주 재배 토양의 우점종과 비율은 거의 일정하게 유지되었다. 근권 토양 DNA의 DGGE 분석을 통해 형질전환 삼각주와 비형질전환 삼각주 토양 미생물 군집의 profile 변화는 나타나지 않았다. 형질전환 삼각주와 비형질전환 삼각주 재배 토양의 화학성은 차이가 없었다. 형질전환 삼각주에 도입된 유전자로 토양 DNA에 대한 PCR 분석결과, 도입 유전자의 잔존성이 길지 않아 수평적 유전자 이동 가능성은 희박할 것으로 추정되었다.