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http://dx.doi.org/10.4014/mbl.1511.11016

Identification of a Newly Isolated Protease-producing Bacterium, Bacillus subtilis FBL-1, from Soil  

Kim, Mina (Department of Food Science and Technology, Yeungnam University)
Si, Jin-Beom (Department of Food Science and Technology, Yeungnam University)
Wee, Young-Jung (Department of Food Science and Technology, Yeungnam University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.2, 2016 , pp. 185-193 More about this Journal
Abstract
A novel proteolytic bacterium was isolated from soil at Yeungnam University, South Korea. The strain, named FBL-1, was rod-shaped with a smooth surface. Biolog and API 50CHB test results revealed that strain FBL-1 was a Bacillus species. Based on 16S rDNA sequencing and chemotaxonomic characterization, the strain was identified as Bacillus subtilis because it had the highest homology with Bacillus subtilis subsp. subtilis NCIB 3610 (99.5%). In liquid culture at 37℃ with shaking at 200 rpm, fructose and yeast extract were found to be the best carbon and nitrogen sources, respectively, for cell growth and protease production. The highest protease activity (451.640 U/ml) was obtained when the strain was cultured in medium containing 20 g/l of fructose and 5 g/l of yeast extract. Although further studies are needed to characterize the protease and enhance its activity, the newly isolated protein-degrading B. subtilis FBL-1 can be applicable for the production of peptides and for the degradation of proteins in various industries.
Keywords
Bacillus subtilis; identification; isolation; protease; soil bacterium;
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