• 한국환경과학회지
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• 제15권3호
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• pp.279-286
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• 2006

Electrokinetic technique was considered in removing arsenic from the abandoned mining tails. In order to estimate the removal characteristics of arsenic, the sequential extraction analysis and desorption experiment were carried out prior to the application of electrokientic process. The result of sequential extraction analysis indicated that the water soluble and exchangeable fraction, easily leachable to ground water, were very low as much as about 2.5% and the fraction except residual (38.3%), possibly extractable under very acidic or alkalic environment, was about 59%. In the result of desorption test using four different kinds of electrolytes, the mixture of citric acid and sodium dodecyl sulfate (SDS) showed the highest desorption efficiency as much as 77.3%. The removal efficiencies of arsenic from mining tailings by electrokinetic process under the different electrolyte environments were slightly low and resulted in the following order: citric acid + SDS (18.6%) > 0.1 $NHNO_3$ (8.1%) > HAc (7.4%) > Distilled water(6.6%). Also, arsenic in soil matrix was moved favorably in the direction of anodic rather than cathodic region, which is opposite trend with cationic metal ions generally existing in soil, because anionic form of arsenic is dominated in acidic soil caused by the movement of acid front form anode.

• 한국염색가공학회지
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• 제15권6호
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• pp.8-17
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• 2003

In order to investigate the possibility of PVA particle as toner, PVA/PVAc particle was manufactured. Fine spherical PVAc particle with emulsifier SDS(sodium Dodecyl Sulfate) and initiator V-50(2,2'- azo bis(2-amidinopropane) dihydrochloride) was manufactured by emulsion polymerization. And then, the PVAc was carried with surface saponification. PVA/PVAc skin core structured particle was obtained under optimum saponification condition. PVA skin side in manufactured PVA/PVAc particles was dyed with 1:2 metal complex type C. I. Acid Yellow 235 and then the dyed PVA particles were observed with a optical microscope. Under given polymerization condition such as SDS concentration, $1.62\times{10}^{-2} \;mol/lH_2O$, V-50 concetration, $3.7\times{10}^{-3}\;mol/lH_2O$ and temperature $50^\circ{C}$ , the high molecular weight of PVAc with Pn 13,900 and PVA with Pn 3,400 was produced. The particle distribution of obtained PVAc microspheres was appeared highly at 60 and $100\mu{m}$, respectively.

• 한국균학회지
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• 제32권2호
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• pp.125-129
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• 2004

섬유소분해효소(CMCase)를 산업적으로 이용하기 위한 기초자료를 제공하고자 섬유소분해능이 우수한 L. japonicus로부터 CMCase를 분리 정제하였다. L. japonicus의 배양액으로부터 4단계를 거쳐 분자량이 42 kDa인 CMCase를 분리 정제하였다. 이 효소는 pH 6.0에서 최적의 활성을 보여주는 acidic CMCase로서 $30^{\circ}C$에서 최대 활성을 나타냈다. EDTA에 의해 활성이 저해되는 것으로 보아 metalloenzyme으로 추정되며, SDS에 의해 저해되는 것으로 보아 S-S기를 갖고 있는 효소로 판단된다. $Al_{2}(SO_{4})_{3}$와 $BaCl_{2}$에서는 효소 활성이 높았으나 그 이외의 금속염에서는 효소 활성이 낮았다.

• 설비공학논문집
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• 제23권1호
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• pp.47-53
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• 2011

In this study, the characteristics of multi-walled carbon nanotubes added nanofluid were investigated. Sodium Dodecyl Sulfate, SDS, and Polyvinylpyrrolidone, PVP, were employed as the dispersant. SDS or PVP was added in pure water. And then, 0.0005, 0.001, 0.002, 0.003. 0.004, 0.005,0.01, and 0.02 vol% of CM-95 and CM-100 were dispersed respectively. The thermal conductivity and the viscosity were measured with a transient hot-wire instrument built for this study and the DV II+ Pro viscometer. The results showed that PVP had good thermal conductivity at 300 wt% and the thermal conductivity of CM-100 was better than that of CM-95. However, the viscosity of CM 100 was higher than that of CM 95.

• 한국어병학회지
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• 제6권2호
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• pp.87-92
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• 1993

1. 1990년 2월 강원도 삼척군에 소재한 송어양식장에서 전염성 조혈기 괴사증 증상으로 산천어 자치어가 대량폐사하였다. 2. 폐사한 산천어 치어로부터 세포배양법에 의해 전염성 조혈기 괴사증 바이러스가 분리되었다. 3. 분리된 바이러스는 구조단백의 크기와 항원성에 있어 미국에서 분리된 RB-76 바이러스주(electro-pherotype 1)와 유사하였다.

• Environmental Engineering Research
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• 제14권1호
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• pp.63-67
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• 2009

Escherichia coli K-12 (E. coli K-12) is a representative indicator globally used for distinguishing and monitoring dynamic fates of pathogenic microorganisms in the environment. This study investigated how to most critically quantify lacZ ($\beta$-galactosidase) gene in E. coli K-12 by two different real-time polymerase chain reaction (real-time PCR) in association with three different DNA extraction practices. Three DNA extractions, i.e., sodium dodecyl sulfate (SDS)/proteinase K, magnetic beads and guanidium thiocyanate (GTC)/silica matrix were each compared for extracting total genomic DNA from E. coli K-12. Among them, GTC/silica matrix and magnetic beads beating similarly worked out to have the highest (22-23 ng/${\mu}L$) concentration of DNA extracted, but employing SDS/proteinase K had the lowest (10 ng/${\mu}L$) concentration of DNA retrieved. There were no significant differences in the quantification of the copy numbers of lacZ gene between SYBR Green I qPCR and QProbe-qPCR. However, SYBR Green I qPCR obtained somewhat higher copy number as $1{\times}10^8$ copies. It was decided that GTC/silica matrix extraction or magnetic beads beating in combination with SYBR Green I qPCR can be preferably applied for more effectively quantifying specific gene from a pure culture of microorganism.

• 한국산학기술학회논문지
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• 제14권8호
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• pp.4106-4110
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• 2013

아포리포포린-III (apoLp-III)를 꿀벌부채명나방 종령 유충 혈림프에서 KBr 농도구배 초원심분리와 겔크로마토그래피 (Sephadex G-100)를 이용하여 분리, 정제하였다. 본 연구에서는 아포리포포린-III가 꿀벌부채명나방의 종령 유충 지방체에 의해 흡수되는 지를 조사하였다. 종령 유충 지방체 조직을 FITC로 표지한 아포리포포린-III(FITC-apoLp-III)와 상온에서 30분간 배양하였다. 배양 후 형광현미경과 전기영동을 이용하여 FITC-apoLp-III가 지방체 조직으로 들어가는 지의 여부를 확인하였다. 그 결과 FITC-apoLp-III가 유충 지방체로 흡수된다는 사실을 알 수 있었다.

• Journal of Plant Biology
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• 제38권3호
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• pp.251-258
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• 1995

The soluble acid invertase ($\beta$-D-fructofuranoside fructohydrolase, EC 3.2.1.26) was isolated and characterized from the hypocotyls of mung bean (Phaseolus radiatus L.). The enzyme was purified to apparent homogeneity by consecutive step using diethylaminoethyl (DEAE)-cellulose anion exchange, Concanavalin (Con) A affinity and Sephacryl S-300 chromatography. The overall purification was about 148-fold with a yield of about 15%. The finally purified enzyme exhibited a specific activity of about 139 $\mu$mol of glucose produced mg-1 protein min-1 at pH 5.0 and appeared to be a single protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nondenaturing PAGE. The enzyme had the native molecular weight of 70 kD and subunit molecular weight of 70 kD as estimated by Sephadex G-200 chromatography and SDS-PAGE, respectively, suggesting that the enzyme was composed of a monomeric protein. On the other hand, the enzyme appeared to be a glycoprotein containing N-linked high mannose oligosaccharide chain on the basis of its ability to bind to the immobilized C on A. The enzyme had a Km for sucrose of 1.8 mM at pH 5.0 and maximum activity around pH 5.0. The enzyme showed highest enzyme activity with sucrose as substrate, but the activity was slightly measured with raffinose and cellobise. No activity was measured with maltose and lactose. These results indicate the soluble acid invertase is a $\beta$-fructofuranosidase.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권1호
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• pp.147-153
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• 2011

The Pseudorabies (PR), also called Aujeszky's disease (AD), is an infectious viral disease caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. Pseudorabies virus (PRV) virions contain several envelope glycoproteins. Among them, gB, gC and gD are regarded as the major immunogenic proteins. We expressed these glycoproteins using the bacterial expression system and analyzed recombinant proteins. Expression of glycoproteins gC and gD were observed on SDS-PAGE or Western blot analysis, but gB was not. Optimal concentration of IPTG and inducing time were determined as 1.0 mM and 4 h, respectively, for the expression of both gC and gD in E. coli. A sodium dodecyl sulfate (SDS) was the most efficient detergent in solubilizing insoluble recombinant protein.

• Applied Biological Chemistry
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• 제29권1호
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• pp.57-61
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• 1986

네품종의 보리(올보리, 영산보리, 사천 6호, 수원 228호)로 부터 Osborne의 방법과 이것을 변형한 전보의 방법으로 단백질을 분획하여 albumin, globulin, hordein 및 glutelin 획분을 얻어 이들 각획분들을 SDS-PAGE로 분석한 결과 품종간 polypeptide 조성 차이를 관찰할 수 있었으며 특히 hordein과 hordein-I을 pH 3에서 PAGE를 수행 하였을 때 각 품종사이의 pattern에 명확한 차이를 볼 수 있었다. 한편, hordein-I의 아미노산조성은 glutamic acid와 proline 함량이 높았으나 품종간의 아미노산조성차이는 거의 볼 수 없었다.