• Applied Biological Chemistry
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• 제39권1호
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• pp.32-38
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• 1996

대두에 함유된 영양저해인자의 하나인 phytic acid를 감소시키기 위해 일정시간 침지시킨 대두를 microwave로 가열처리하여 phytic acid 및 phosphorus의 함량 변화를 관찰하였다. 단원콩, 만리콩 및 Amsoy의 phytic acid 함량은 각각 탈지대두 1 g중에 19.19 mg, 18.38 mg 및 16.73 mg 함유되어 있었다. 가열시간이 길어짐에 따라 콩에 함유된 phytic acid 및 phytate phosphorus 함량은 감소하는 경향을 보여서, 5분 가열시에 단원콩, 만리콩 및 Amsoy의 phytic acid 감소율은 각각 26.29%, 24.85%, 23.72%이었다. 단원콩을 12에서 30분간 autoclaving 처리하였을때 phytic acid 및 phytate phosphorus의 감소율은 18.62%와 16.95%로 microwave로 5분 처리하였을 때가 훨씬 효과적이었다. 대두의 수분함량과 microwave 가열효과와의 관계는 수분함량이 낮을수록 phytic acid의 감소 효과가 컸다. 몇가지 염용액에 12시간 침지시킨후 4분간 가열처리시 phytic acid의 감소율은 2.5% NaCl에서 24.52%, 2% $NaHCO_3$에서 26.62%, 혼합 염용액(M.S.S.)에서 28.47%로 증류수의 21.53%보다 약간 높게 나타났으며, phytate phosphorus 감소율 또한 위의 세가지 염용액에서 26.17%, 24.64% 및 26.60%로서 증류수의 19.73%보다 약간 더 효과가 있는 것으로 나타났다.

• 한국미생물·생명공학회지
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• 제39권2호
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• pp.146-152
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• 2011

본 연구에서는 건조 근채류 절편을 천연과일주스에 침지 후 건조하여 과일주스의 관능성과 유용활성을 동시에 나타내는 근채류 칩 개발을 목표로, 먼저 국내 시판주스 8종에 대한 이화학적 특성과 다양한 생리활성을 조사하였다. 사과, 오미자, 포도, 머루, 오렌지, 토마토, 홍삼 및 흑마늘 주스의 평균 수분함량, pH, brix 및 산도는 각각 $85.59{\pm}5.80%$, $3.90{\pm}0.64$, $12.19{\pm}4.70%$, 및 $0.49{\pm}0.19%$였다. 총폴리페놀 함량의 경우, 흑마늘 주스 및 포도 주스가 각각 1.50 및 1.21 mg/ml으로 8종 주스 평균인 0.57 mg/ml보다 매우 높았으며, 총플라보노이드 함량도 흑마늘 주스 및 포도 주스가 가장 높게 나타났다. 주스들의 항혈전 활성 평가 결과, 오미자 주스에서만 아스피린 1.5 mg/mL에 상당하는 활성이 인정되었으며, 항균 활성 평가 결과, 오미자, 포도, 및 오렌지 주스에서 우수한 항세균 활성이 나타났으나 항진균 활성은 모든 주스에서 인정되지 않았다. 항산화 활성 평가 결과, 포도>흑마늘>오렌지>머루>토마토>사과>오미자>홍삼 주스 순으로 나타났으며, nitrite 소거능은 머루>흑마늘>포도>홍삼 의 순으로 나타났다. 본 연구결과는 항혈전 활성이 우수한 오미자 주스, 항산화 활성이 우수한 포도 및 흑마늘 주스를 이용한 관능성과 기능성이 강화된 근채류 칩 제품개발의 기초자료로 이용될 것이다.

• Journal of the Korean Wood Science and Technology
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• 제14권4호
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• pp.21-28
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• 1986

Plywood used for construction as a decorative inner material is inflammable and can fire accident, causing destruction of human life and property. In this study, 3.5mm Kapur plywoods were soaked in the 23% monoammonium phosphate solutions by cold soaking method 3, 6, 9hrs and hot-cold bath method for 3/3hrs, and redrying was carried out by press-drying at the platen temperature of 110, 130, 160, 180$^{\circ}C$, and then fire test was carried out to investigate burning point, flame exhausted length, frame spread length, back side carbonized area and weight loss. The results are as follows; 1. In cold soaking method for 3, 6, 9hrs. retentions of monoammonium phosphate were 0.377, 0.448, 0.498kg/(30cm)$^3$ respectively, and in hot-cold bath method for 3/3hrs, the retention was 1.331kg(30cm)$^3$ that exceeded the minimum retention 1.124kg/(30cm)$^3$. 2. Correlation coefficients among the variable were shown in table 2. From the table, it could be recognized that there were close negative correlations between the treatment and burning point, flame spread length, back side carbonized area, flame exhausted time and weight loss, and there was negative correlation between treating time and back side carbonized area, but there was positive correlation between platen temperature and burning point. 3. From table 3, it can be observed that there were highly significant differences for burning point, flame spread length, flame exhausted time, back side carhonized area, weight loss between treatments. And in 2-way interactions, there were also highly significant for burning point, flame spread length, flame exhausted time, weight loss between time x treatment. 4. It was observed that burning point, flame exhausted time, flame spread length, back side carbonized area, and weight loss in fire-retardant treated plywood were the best effects in fire-retardant treated plywood, water treated plywood and nontreated plywood. In conclusion, I can estimate that absorbed chemical contents by hot-cold bath method for 3/3hrs, have a lot of effects on fire-retardant factors such as burning point, flame spread length, flame exhausted time, backside carbonized area and weight loss, but platen temperatures have a little effects on the fire factors.

• 한국자원식물학회지
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• 제27권5호
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• pp.534-539
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• 2014

본 연구는 식물구계학적 특정종 III 등급이며, 국외반출 시 승인이 필요한 보호종인 등수국의 종자 번식법을 개발하기 위해 수행되었다. 종자의 크기는 $1.36{\times}0.84mm$이며, 1,000립 중은 $2.3{\pm}0.02mg$이었다. 종자는 타원형으로 표면에 주름이 있었으며, 황색의 미립종자였다. 침지처리에 의해 종자의 함수율은 48시간 만에 3.9%에서 31.9%로 급속히 증가하여 최대 함수율을 보였다. 온도 및 광조건에 따른 발아율은 $25^{\circ}C$, 명조건에서 가장 높았으며(78.0%), $GA_3$ 용액에 48시간 처리함으로써 발아율, 발아세 및 $T_{50}$이 매우 향상되었다. 특히 $GA_3$ 처리구는 $100{\sim}500mg{\cdot}L^{-1}$의 농도에서 발아율이 90.0% 이상으로 매우 우수였으며 발아세와 $T_{50}$을 무처리에 비해 향상시킬 수 있었으므로, $GA_3$는 등수국 종자의 발아력을 효과적으로 향상시킬 수 있는 적절한 화학적 처리방법으로 판단되었다.

• 한국자원식물학회지
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• 제30권1호
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• pp.50-57
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• 2017

본 연구는 식의약소재로 활용 가치가 높은 늘어진장대(Arabis pendula L.)의 대량증식을 위한 기초연구로 종자의 형태적 특성과 발아특성을 규명하고, 최적 발아조건을 규명하고자 수행되었다. 늘어진장대 종자는 종피에 날개가 있는 원형으로 색은 진갈색이며, 너비 1.47 mm, 길이 1.25 mm이고, 배는 굴곡형이며 무배유종자이다. 온도와 광조건 실험에서, 종자 발아는 $20^{\circ}C$, 암조건에서 가장 우수하였다(49.7%). 그러나 암조건에서는 유묘가 약하고 웃자랐으며, 자엽이 작고 말려있는 특징을 보였다. 종자 발아와 유묘의 생육을 촉진하기 위하여 종자를 다양한 농도의 $GA_3$ 희석액(0, 200, 500, 1,000 mg/L)에 침지하여 전처리 한 후 파종하였다. 연구 결과, $GA_3$ 희석액 500 mg/L처리구에서 종자 발아율이 가장 높고(97.3%), 발아 소요기간이 짧았으며(8.1일), 유묘의 생육 또한 왕성하였다. 연구의 결과, 늘어진장대 종자의 최적 발아조건은 $GA_3$ 500 mg/L희석액에 침지처리($4^{\circ}C$, dark, 24시간)하여 $20^{\circ}C$에 파종하여 15일 동안 발아시키는 것으로 확인되었다.

• 한국수산과학회지
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• 제10권1호
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• pp.11-15
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• 1977

Brownish discoloration develops very rapidly in the storage of dried oyster. This undesirable browning is mainly caused by the series of reactions of sugar-amino condensation, enzymatic oxidation of tyrosine and/or the oxidative rancidity of lipids in the tissue of oyster. Sulfites are commonly used as inhibitors for Maillard type browning reactions in agricultural products. The inhibitory effect of sulfite treatment on canned oysters was also confirmed in some investigations. The results suggested that sulfites not only work on blocking tile amadori rearrangement but also on the reduction of free tyrosine which retards the progress of enzymatic oxidation of tyrosine tyrosinase. In this paper, the effect of sodium sulfite treatment on the reduction of reducing sugar and free tyrosine as a function i)f browning inhibition in oyster was tested and other treatment with glucose-oxidase and yeast were also applied. In preparation of samples, fresh oysters were soaked in sodium sulfite solution by various concentration for different treating times, washed in running water to remove the sulfite residue, and finally dried in the shade. In the result, the treatment of sodium sulfite was certainly effective on the reduction of both free tyrosine and reducing sugars in fresh oyster. The best results were obtained by the treatment of 0.5M sodium sulfite solution for 60 minutes each for soaking and washing. Treatment with, glucose-oxidase and yeast solutions, however, did appear somewhat effective but it required so much time for a certain effect that it seemed not practically applicable.

• Journal of the Korean Wood Science and Technology
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• 제11권3호
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• pp.39-48
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• 1983

본(本) 연구(硏究)는 내화처리(耐火處理) 파티클보오드의 휨강도(强度)와 물리적(物理的) 성질(性質)을 조사검토(調査檢討)함으로써 내화처리(耐火處理) 파티클보오드의 제조가능성(製造可能性)을 구명(究明)하고져 실시하였다. 본(本) 연구(硏究)에서는 목재(木材)파티클을 5, 10, 15, 그리고 20%농도(濃度)의 황산암모늄과 미나리스(Minalith) 용액(溶液)에 침적(沈積)시킨후 건조(乾燥)시켜서 제조(製造)하였다. 제조(製造)된 파티클보드의 휨강도(强度)와 물리적(物理的) 성질(性質)을 조사(調査)한 결과(結果)에 따르면 휨강도(强度)에서 파괴계수(破壞係數)와 탄성계수(彈性係數)는 한국공업표준규격(韓國工業標準規格) KS F 3104의 type 100수준(水準) (100kgf/$cm^2$, $1.5{\times}10^4kgf/cm^2$)을 만족 시켰으나 두께팽창율(膨脹率)은 모두 이 규격수준(規格水準)(12%)에 이르지 못하였다. 또한 박리저항(剝離抵抗)은 미나리스의 15와 20%농도(濃度)의 경우를 제외(除外)하고는 모두 한국공업표준규격(韓國工業標準規格) KS F 3104의 type 100 수준(水準)(1.5kgf/$cm^2$을 능가(凌駕)하였다.

• 환경위생공학
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• 제6권2호
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• pp.89-108
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• 1991

Organophosphorus pesticide residues such as Diazinon, Fenitrothion and EPN in apple and effect of storage peeling and washing on removal of the residues from apple soaked in 3 kinds of pesticides solutions for 20 seconds were studed with gas chromatography-nitrogen phosphorus detecter(GC-NPD). Result obtained are as follows : 1) Average concentrations of DiaEinon, Fenitrothion, and EPN detected in apple of control group were 0.022, 0.007 and 0.008 ppm respectively. 2) Decreasing rates of Diazinon on 7 th, 14 th, 21 st, 28 th, and 35 th day after soaking apple on the pesticide solution were 41.3% , 68.6% , 87.0%, 96.9% and 99.5% respectively. In case of Fenitrothion were 46.9%, 66.3%, 84.9%, 93.2% and 97.3% and EPN were 45.7 %, 76.2%, 85.4%, 95.7% and 99.4% respectively. 3) The removal rate of Diazinon, Fenitrothion and EPN by washing with water alone were 93.7%, 70.6% and 51.5% respectively, and 97.1% , 78.4% ailed 76.5% by washing with 0.2% detergent solution respectively. The results obtained in this study have show that 3 kinds of pesticides detected in app- les were below the Korean standard for residual pesticides and pesticides contaminated in apples were decreased in considerable degree by washing with water and 2% detergent solution and removed almost completely after storage for 35 days (5 weeks). Therefore, it would be concluded that washing and peeling will be the most effective way for safely because more than 90% of pesticide exist in peel.

• 한국약용작물학회지
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• 제26권5호
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• pp.362-369
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• 2018

Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

• Journal of Crop Science and Biotechnology
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• 제11권4호
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• pp.249-256
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• 2008

The objective of this work was to evaluate the germination of 12 rice genotypes under propionic acid stress, a phytotoxic compound produced in low drainage soils with high organic matter content. The tests were conducted with the first count of germination (PCG) and germination (G) of the genotypes subjective to 0, 3, 6, and 9 mM propionic acid concentrations. The seeds of each genotype were placed in germitest paper pre-soaked in treatment solutions forming individual bags. The germination was performed at $25^{\circ}C$ and the counts were carried out at 7 (PCG) and 14 days (G). A factorial random block design was performed with four replications of 50 seeds per genotype. Our study revealed that doses up to 9 mM propionic acid in the pre-soaking solution were efficient for genetic variability studies involving the character germination in rice; genetic variability for germination was detected in the collection of rice genotypes when subjected to propionic acid toxic effects. The genotypes Guichow, Dawn, and Toride-1 showed germination stability when subjected to increasing levels of propionic acid, and genotypes originated from irrigated system-cultivation performed better when subjected to propionic acid stress. These three genotypes will be a good biological material to for enhance the resistance to phytotoxic compounds in rice.