• 생명과학회지
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• 제29권11호
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• pp.1179-1191
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• 2019

암세포는 epithelial mesenchymal transition (EMT)를 통해 tumor invasion과 metastasis가 일어나며, 또한 정상세포와 다른 oncogenic metabolic phenotypes 획득 즉, glycolytic switch 등이 암 발생과 진행에 깊이 연관되어 있음이 잘 알려져 있다. High-mobility group box 1 (HMGB1)은 chromatin-associated nuclear protein으로 알려져 있으나, dying cells 또는 immune cells로부터 방출되기도 한다. 방출된 HMGB1은 damage-associated molecular pattern (DAMP)로서 작용하여 EMT 및 invasion, metastasis를 유도함으로서 tumor progression에 기여한다고 알려졌다. 본 연구에서 HMGB1에 의해 EMT와 glycolytic switch 유도되며, 이 과정은 Snail 의존적임을 확인하였다. 또한 HMGB1/Snail cascade는 COX subunits인 COXVIIa와 COXVIIc의 발현 억제를 통해 mitochondrial repression과 cytochrome c oxidase (COX) inhibition을 유도하였다. HMGB1은 Snail를 통해 glycolytic switch의 주요 효소인 hexokinase 2 (HK2), phosphofructokinase-2/fructose-2,6-bisphosphatase 2 (PFKFB2), phosphoglycerate mutase 1 (PGAM1)의 발현을 증가시켰다. 이들 효소는 glycolytic switch에 중요하게 관여하는 것으로 알려져 있다. 이들 해당과정의 효소들을 knockdown한 결과 HMGB1에 의한 EMT를 억제함으로써 glycolysis와 HMGB1-induced EMT가 밀접하게 연관되어 있을 제시하였다. 이상의 연구 결과들은 HMGB1/Snail cascade가 EMT 및 glycolytic switch, mitochondrial repression에 중요하게 작용할 것임을 시사한다.

• 한국패류학회지
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• 제27권3호
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• pp.191-192
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• 2011

Pulmonate snail, Onchidium hongkongensis from muddy flat of Muan-gun was recorded as new to the Korean molluscan fauna. Shell-less body light-gray or reddish-gray in colour, oval shaped with numerous pustules on dorsal surface usually covered with mud. Black pair of tentacles with terminal eyes. Including the new record in this study, the family Onchidiidae 2 genera and 2 species in the Korean waters.

• 한국패류학회지
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• 제27권2호
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• pp.87-90
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• 2011

The karyotypes of Korean endemic land snail, Koreanohadra koreana, using air-drying method wereinvestigated. Somatic cells of this species had 2n = 58. Karyotypes were also analysed with 16 metacentric, 12 submetacentric and one subtelocentric chromosome pairs. Observed chromosomes ranged from 2.6 to $8.9{\mu}m$ and the total length was $122.3{\mu}m$. This is the second report on the chromosome numbers and the karyotype of K. koreana.

• 대한의용생체공학회:의공학회지
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• 제28권1호
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• pp.24-28
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• 2007

Presuming that firing neurons have motions inside the MRI magnet due to the interaction between the neuronal magnetic field and the main magnetic field, we applied motion encoding gradients to dissected snail ganglia to observe faster responding MRI signal than the BOLD signal. To activate the snail ganglia in synchronization with the MRI pulse sequence, we used electrical stimulation with the frequency of 30 Hz and the pulse width of 2s. To observe the fast responding signal, we used the volume selected MRI sequence. The magnetic resonance signal intensity, measured with 8 ms long motion encoding gradient with a 20mT/m gradient strength, decreased about $3.46{\pm}1.48%$ when the ganglia were activated by the electrical stimulation.

• 한국패류학회지
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• 제9권2호
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• pp.57-67
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• 1993

A cladistic analysis for phylogenetic relationship among 3 freshwater planorbid snail species was carried out on the basis of their conchological and anatomical characteristics.The intestinal loop in the digestive systims was mainly distinguishable among 3 species of the planorbid snails. The loops in Hippeutis cantori and Segmentina hemisphaerula coiled around stomach, extended down to the distal part of the liver and finally connected with the anus, while the loop in Gyraulus convexiusculus was short and small, and connected directly to the anus after coiling around the stomach. However, the intestinal loop of Hippeutis cantori was the largest among those of 3 target snail species. In the main morpholohical differences of the riproductive systems, Gyraulus convexiusculus uniquely had a calcareous stylet in the penis sheath. Although there was no stylet in the penis sheath in the other snail species, the penis shesths connected with 1 and 2 flagella in Segmentina hemisphaerula and in Hippeutis cantori, respectively were different from each other. Two planorbid species, Hippeutis cantori and Segmintina hemisphaerula have a more recent phylogenetic ancestor out three species employed including Gyraulus convexiusculus.

• Parasites, Hosts and Diseases
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• 제39권3호
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• pp.247-253
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• 2001

Three freshwater snail species of the family Lymnaeidae have been reported from Korea, Radix auricularia coreana, Austropeplea ollula and Fossaria truncatula. Out of 3 lymnaeid snail species, A. ollula was naturally infected with the Echinostoma cinetorchis cercariae (infection rate : 0.7%). In the experiments with the laboratory-bred snails, F. truncatula as well as A. ollula was also susceptible to the E. cinetorchis miracidia with infection rates of 25% and 40%, respectively. All of three lymnaeid snail species exposed to the E. cinetorchis cercariae were infected with the E. cinetorchis metacercariae. It is evident that A. ollula acts as the first molluscan intermediate host of E. cinetorchis in Korea, and F. truncatula may be a possible candidate for the first intermediate host of this intestinal fluke. Also, three lymnaeid snail species targeted were experimentally infected with E. cinetorchis metacercariae.

• 한국패류학회지
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• 제8권1호
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• pp.21-28
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• 1992

Field investigations were made in 1991 and 1992 in Kwangju and the western and central Chonnam area to determine the distribution of Lymnaea viridis, the intermediate host of Fasciola hepatica, and to study their life history during the different seasons. It had a wide distribution, existing all over the places, and the principal habitats of the snail were rice paddies. The life cycle was determined by making frequent visits to a series of habitats. The snsils began to produce egg masses in April, and egg production was the highest in May and June. Egg laying was continued but decreased in summer. There was sudden decrease in egg production in October when the temperature was below 2$0^{\circ}C$. The number of snails in July. Under the unfavourble conditions the snail either crawle up on the snail to aestivation. At low temperature below 8$^{\circ}C$ the snails hibernated inthe mud of the habitat. It was found in some habitats thao the snails were loving together with L. auricularia and Physa s pp. which indicates the neccessity of differentiation among them.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2008년도 추계학술대회 논문집
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• pp.187-188
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• 2008

• 한국패류학회지
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• 제26권4호
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• pp.275-283
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• 2010

Many freshwater snail taxa are difficult to identify using morphological traits due to phenotypic plasticity. However, using of molecular DNA marker in combination with morphological traits can provide a reliable means for discriminating among freshwater snail taxa including cryptic species. To discriminate among Korean freshwater snail taxa and resolve their systematic relationships, wesequenced a fragment of mtDNA cytochrome oxidase I (COI) gene from 82 specimens collected from ten different sites distributed along the Korean peninsula. We identified more than seven freshwater snail taxa including cryptic species in Korea. Whereas traditional shell morphology of freshwater snails offers only weak discriminatory power for recognizing 'good' taxa, DNA sequence data provided positive and reliable identification. In addition, a major Semisulcospira clade was clearly separated from the remaining lineages observed including cryptic species. However, a phylogenetic tree inferred from the COI gene data did not fully resolve systematic relationships among pleurocerid taxa in Korea. Establishing more robust shell characteristics for identifying taxa unambiguously and hence improving traditional key shell morphology characters for freshwater snail species is an urgent requirement and will require more rigorous examination of all nominal taxa. While molecular data generated here will be useful for species identification and for describing the systematic relationships among Korean freshwater snails, further analysis will be required.

• 한국잡초학회지
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• 제32권3호
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• pp.273-279
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• 2012

본 농가 실증시험에서 대조구(관행 이앙재배 및 화학적 제초제 2회 처리방법)에 비하여 무논써레 직후 왕우렁이 치패(새끼)를 ha당 12kg 처리(1차)와 무논점파(철분코팅볍씨이용) 후 유묘 2엽기경(파종 후 15일) 왕우렁이 새끼(치패) ha당 10kg(2차) 체계투입으로 100%의 높은 논 잡초방제 효과가 인정되었다. 벼 유묘의 피해정도는 하위엽에 3%정도의 피해를 보였으나 뿌리가 활착된 상태에서 후속적인 생장점을 가진 엽의 지속적인 출현으로 벼의 초장과 분얼수에는 유의적인 피해차이가 나타나지 않았다. 새끼(치패) 왕우렁이를 투입하여 무논점파재배를 한 농사실증시험에서 최종적인 수량 및 품질에서는 관행 이앙재배와 통계적으로 유의적인 차이가 없는 것으로 조사되었다.