• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.325-334
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• 2007

Recently, quorum sensing has been implicated as an important global regulator controlling the production of numerous virulence factors such as capsular polysaccharides in bacterial pathogens. The nucleotide and deduced amino acid sequences of smcR, a homolog of V. harveyi luxR identified from V. vulnificus ATCC29307, were analyzed. The amino acid sequence of SmcR from V. vulnificus was 72 to 92% similar to those of LuxR homologs from Vibrio spp. Functions of SmcR were assessed by the construction of an isogenic mutant, whose smcR gene was inactivated by allelic exchanges, and by evaluating its phenotype changes in vitro and in mice. The disruption of smcR resulted in a significant alteration in biofilm formation, in type of colony morphology, and in motility. When compared with the wild-type, the smcR mutant exhibited reduced survival under adverse conditions, such as acidic pH and hyperosmotic stress. The smcR mutant exhibited decreased cytotoxic activity toward INT 407 cells in vitro. Furthermore, the intraperitoneal $LD_{50}$ of the smcR mutant was approximately $10^2$ times higher than that of parental wild-type. Therefore, it appears that SmcR is a novel global regulator, controlling numerous genes contributing to the pathogenesis as well as survival of V. vulnificus.

• The Journal of the Korea Contents Association
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• v.12 no.7
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• pp.232-239
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• 2012

The purpose of this study was to investigate the relationship among functional evaluation systems, the Selective Motor Control Scale (SMC scale), the Gross Motor Function Classification System (GMFCS), the Gross Motor Function Measure (GMFM) and Activities of daily living in children with spastic cerebral palsy and to provide the foundation data about SMC scle for evaluation system of abilities of selective motor control in children with spastic cerebral palsy. For this, sixty eight children with spastic cerebral palsy were participated in this study. The children were evaluated by using the SMC scale for their selective motor control ability and by using the GMFCS and GMFM for their gross motor function. The activities of daily living were assessed by using the Functional Independence Measure of Children (WeeFIM). There were a significant correlation between the SMC scale and the GMFCS (r = -.485, p < .05). The good correlation between the SMC scale and GMFM was found (r = .482, p < .05). The activities of daily living were not a significant correlation with SMC scale (r = .019, p > .05). The SMC scale in practice will provide usefulness for assessment of abilities of selective motor control in children with spastic cerebral palsy.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1197-1206
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• 2005

Vibrio vulnificus is an opportunistic pathogen that causes a septicemia and expresses numerous virulence factors, in which luxS and smcR are genes encoding for components responsible for quorum-sensing regulation. In the present study, null mutants were constructed with lesions in each or both of these two genes from the V. vulnificus Vv$\Delta$Z strain, which is a lacZ$^{-}$ and chloramphenicol/streptomycin-resistant derivative of the wild-type ATCC29307 strain, and their phenotypes related to virulence were compared with those of the parental cells. $LD_{50}$ and histopathological findings of luxS-, smcR-, or luxS- smcR- deficient mutant were not different from those of the parent strain, a lacZ-deficient streptomycin-resistant strain in mice. However, time of death in mice was delayed, and numbers of bacteria survived in bloodstream after intraperitoneal injection in mice were decreased by mutation, especially luxS and smcR double mutant (VvSR$\Delta$ZSR). These phenomena were supported by increased serum sensitivity and delayed bacterial proliferation in both murine blood and iron-restricted medium. These results suggest that the luxS and luxR homologous genes in V. vulnificus could playa role in bacterial survival in host by enhancing proliferation and adjusting to changed environment.

• BMB Reports
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• v.46 no.11
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• pp.550-554
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• 2013

The platelet-derived growth factor (PDGF) signaling pathway is essential for inducing a dedifferentiated state of vascular smooth muscle cells (VSMCs). Activation of PDGF inhibits smooth muscle cell (SMC)-specific gene expression and increases the rate of proliferation and migration, leading to dedifferentiation of VSMCs. Recently, microRNAs have been shown to play a critical role in the modulation of the VSMC phenotype in response to extracellular signals. However, little is known about microRNAs regulated by PDGF in VSMCs. Herein, we identify microRNA- 15b (miR-15b) as a mediator of VSMC phenotype regulation upon PDGF signaling. We demonstrate that miR-15b is induced by PDGF in pulmonary artery smooth muscle cells and is critical for PDGF-mediated repression of SMC-specific genes. In addition, we show that miR-15b promotes cell proliferation. These results indicate that PDGF signaling regulates SMC-specific gene expression and cell proliferation by modulating the expression of miR-15b to induce a dedifferentiated state in the VSMCs.

• Transactions of the Korean Society of Mechanical Engineers
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• v.13 no.3
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• pp.472-478
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• 1989

Using the SMC(sheet moulding compound) composite material consisting of E-glass chopped strand mat and unsaturated polyester resin, three-point bending tests are carried out to evaluate the elasto-plastic fracture toughness by means of R curve. The crack extension is experimentally observed with the ink staining method. The point of stable crack growth is discussed in consideration of the load-load point displacement curve, the damage behavior of the notch vicinity, and the R-curves. The damage zone of the notch vicinity was composed of the initiation and growth of subcracks as well as those of the main crack. The point of stable crack growth can be defined as the inflection point in the R curves and its point also concurrs with the proportional limit on the load-load point displacement curve.

• Journal of the Korea Institute of Building Construction
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• v.22 no.4
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• pp.359-370
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• 2022

Despite continuous efforts to reduce on-site safety accidents, the construction industry remains a high-risk sector with a high rate of fatal accidents. Design for Safety(DFS), which manages safety risk factors at the design stage, is being used as a method to lower the construction safety accident rate. However, due to limited knowledge, designers are unaware of the design-results risks during the different of the project lifecycle, including construction, operation, and maintenance. Effective DFS can be conducted if the designer understands the effect of the building shape on the safety accident rate and corresponding safety management cost(SMC). The cost of safety facilities such as fall prevention nets and safety fences will vary depending on the shape of the building. This study analyzes the outer perimeter length's impact on SMC. Following the data collection from 21 projects for this study, an analysis was conducted using the independent variables of the building perimeter(BP), building shape factor(BSF), and building area(BA), the dependent variable of SMC. The correlation R2 was found to be 0.876, 0.801 and 0.792 between the BP and SMC, BSF and SMC, BA and SMC, respectively, indicating that these factors were closely related.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2000.05a
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• pp.249-255
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• 2000

HRIV(Hybrid Rule-Interval Variation) method is presented to stabilize a class of nonlinear systems, where SMC(Sliding Mode Control) and ADC (ADaptive Control) schemes are incorporated to overcome the unstable characteristics of a conventional FLC(Fuzzy Logic Control). HRIV method consists of two modes: I-mode (Integral Sliding Mode PLC) and R-mode(RIV method). In I-mode, SMC is used to compensate for MAE(Minimum Approximation Error) caused by the heuristic characteristics of FLC. In R-mode, RIV method reduces interval lengths of rules as states converge to an equilibrium point, which makes the defined Lyapunov function candidate negative semi-definite without considering MAE, and the new uncertain parameters generated in R-mode are compensated by SMC. In RIV method, the overcontraction problem that the states are out of a rule-table can happen by the excessive reduction of rule intervals, which is solved with a dynamic modification of rule-intervals and a transition to I-mode. Especially, HRIV method has advantages to use the analytic upper bound of MAE and to reduce Its effect in the control input, compared with the previous researches. Finally, the proposed method is applied to stabilize a simple nonlinear system and a modified inverted pendulum system in simulation experiments.

• Mycobiology
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• v.41 no.4
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• pp.214-220
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• 2013

This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost (SMC) of Pleurotus ostreatus, P. eryngii, and P. cornucopiae. Optimal enzyme recovery was achieved when SMCs were extracted with 50 mM sodium citrate (pH 4.5) buffer at $4^{\circ}C$ for 2 hr. Amylase, cellulase, and xylanase activities showed high values in extracts from P. ostreatus SMC, with 2.97 U/g, 1.67 U/g, and 91.56 U/g, respectively, whereas laccase activity and filter paper degradation ability were highest in extracts from P. eryngii SMC, with values of 9.01 U/g and 0.21 U/g, respectively. Enzymatic activities varied according to the SMCs released from different mushroom farms. The synthetic dyes remazol brilliant blue R and Congo red were decolorized completely by the SMC extract of P. eryngii within 120 min, and the decolorization ability of the extract was comparable to that of 0.3 U of commercial laccase. In addition, laccase activity of the SMC extract from P. eryngii was compared to that of commercial enzymes or its industrial application in decolorization.

• Composites Research
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• v.31 no.1
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• pp.8-11
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• 2018

Sheet molding compound (SMC) is one of the most economical fiber reinforced composite fabrication processing for automotive applications. In this study, we studied the optimum formulation for the production of SMC which shows low specific gravity without lowering the mechanical properties by using glass bubble (GB) which is a low specific gravity filler and glass fiber (GF) as a reinforcing material. The tensile strength increased with the increase of the GF in the SMC, and the specific gravity decreased with the increase of the GB. The synergistic effect of improving the mechanical properties as the specific gravity is lowered is found in the optimum formulation. The synergy effect was confirmed by the internal structure analysis that the dispersion effect of the crack propagation of the GB and the improvement of the binding force between the fiber and the matrix due to the incorporation of the GB.

• Journal of Institute of Control, Robotics and Systems
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• v.20 no.7
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• pp.701-705
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• 2014

This article presents a comparison among several yaw and roll motion controllers for vehicle rollover prevention. In the previous research, yaw and roll motion controllers can be independently designed for rollover prevention. Following this idea, several yaw and roll motion controllers are designed and compared in terms of rollover prevention. For the yaw motion control, PID, LQR, SMC (Sliding Mode Control) and TDC (Time-Delay Control) are adopted. For the roll motion control, LQR, LQ SOF (Static Output Feedback) control, PID, and SMC are adopted. To compare the performance of each controller, simulation is performed on a vehicle simulation package, CarSim$^{(R)}$. From simulation, TDC and LQ SOF are the best for yaw and roll motion control, respectively.