• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.325-334
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• 2007

Recently, quorum sensing has been implicated as an important global regulator controlling the production of numerous virulence factors such as capsular polysaccharides in bacterial pathogens. The nucleotide and deduced amino acid sequences of smcR, a homolog of V. harveyi luxR identified from V. vulnificus ATCC29307, were analyzed. The amino acid sequence of SmcR from V. vulnificus was 72 to 92% similar to those of LuxR homologs from Vibrio spp. Functions of SmcR were assessed by the construction of an isogenic mutant, whose smcR gene was inactivated by allelic exchanges, and by evaluating its phenotype changes in vitro and in mice. The disruption of smcR resulted in a significant alteration in biofilm formation, in type of colony morphology, and in motility. When compared with the wild-type, the smcR mutant exhibited reduced survival under adverse conditions, such as acidic pH and hyperosmotic stress. The smcR mutant exhibited decreased cytotoxic activity toward INT 407 cells in vitro. Furthermore, the intraperitoneal $LD_{50}$ of the smcR mutant was approximately $10^2$ times higher than that of parental wild-type. Therefore, it appears that SmcR is a novel global regulator, controlling numerous genes contributing to the pathogenesis as well as survival of V. vulnificus.

• 한국콘텐츠학회논문지
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• 제12권7호
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• pp.232-239
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• 2012

이 연구는 경직형 뇌성마비 아동의 운동 손상를 측정하는 도구로서 선택적 운동조절능력 척도에 대한 기초 자료를 제공하고 선택적 운동조절능력과 대동작 기능 분류 체계, 대동작 기능 평가 결과, 그리고 일상 생활동작 평가 결과 사이의 상관성을 알아보는데 그 목적이 있다. 이를 위해 68명의 경직형 뇌성마비 아동을 대상으로 선택적 운동조절능력, 대동작 기능 분류 체계 평가, 대동작 기능 평가, 그리고 일상생활동작 평가를 실시하고 상관을 알아보았다. 그 결과 선택적 운동 조절 척도 등급은 대동작 기능 분류 체계 평가(r = -.485)와 대동작 기능 평가(r = .482)와 유의한 상관을 보였다(p<.05). 하지만 일상생활동작과는 유의한 상관을 보이지 않았다(p>.05). 이는 이 척도가 경직형 뇌성마비 아동의 운동 손상 중 선택적 운동조절능력을 평가하는 평가도구로써 유용하게 사용될 수 있음을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1197-1206
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• 2005

Vibrio vulnificus is an opportunistic pathogen that causes a septicemia and expresses numerous virulence factors, in which luxS and smcR are genes encoding for components responsible for quorum-sensing regulation. In the present study, null mutants were constructed with lesions in each or both of these two genes from the V. vulnificus Vv$\Delta$Z strain, which is a lacZ$^{-}$ and chloramphenicol/streptomycin-resistant derivative of the wild-type ATCC29307 strain, and their phenotypes related to virulence were compared with those of the parental cells. $LD_{50}$ and histopathological findings of luxS-, smcR-, or luxS- smcR- deficient mutant were not different from those of the parent strain, a lacZ-deficient streptomycin-resistant strain in mice. However, time of death in mice was delayed, and numbers of bacteria survived in bloodstream after intraperitoneal injection in mice were decreased by mutation, especially luxS and smcR double mutant (VvSR$\Delta$ZSR). These phenomena were supported by increased serum sensitivity and delayed bacterial proliferation in both murine blood and iron-restricted medium. These results suggest that the luxS and luxR homologous genes in V. vulnificus could playa role in bacterial survival in host by enhancing proliferation and adjusting to changed environment.

• BMB Reports
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• 제46권11호
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• pp.550-554
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• 2013

The platelet-derived growth factor (PDGF) signaling pathway is essential for inducing a dedifferentiated state of vascular smooth muscle cells (VSMCs). Activation of PDGF inhibits smooth muscle cell (SMC)-specific gene expression and increases the rate of proliferation and migration, leading to dedifferentiation of VSMCs. Recently, microRNAs have been shown to play a critical role in the modulation of the VSMC phenotype in response to extracellular signals. However, little is known about microRNAs regulated by PDGF in VSMCs. Herein, we identify microRNA- 15b (miR-15b) as a mediator of VSMC phenotype regulation upon PDGF signaling. We demonstrate that miR-15b is induced by PDGF in pulmonary artery smooth muscle cells and is critical for PDGF-mediated repression of SMC-specific genes. In addition, we show that miR-15b promotes cell proliferation. These results indicate that PDGF signaling regulates SMC-specific gene expression and cell proliferation by modulating the expression of miR-15b to induce a dedifferentiated state in the VSMCs.

• 대한기계학회논문집
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• 제13권3호
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• pp.472-478
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• 1989

본 연구에서는 FRP의 파괴인성평가를 위한 기초적인 자료를 얻기 위하여 FRP 중에서도 널리 사용되고 있는 SMC재를 준비하고 3점 굽힘시험을 실시하여 노치선단에서의 손상거동 및 균열진전저항유선으로부터 안정파괴개시점을 검토하였다.

• 한국건축시공학회지
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• 제22권4호
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• pp.359-370
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• 2022

현장의 안전사고율을 낮추려는 지속적인 노력에도 불구하고 여전히 건설산업은 치명적인 사고율이 높은 고위험군으로 분류된다. 건설 안전사고율을 낮추기 위한 방법 중 하나로 설계 단계에서 안전위해요소를 관리하는 DFS(Design for safety)를 적용하고 있다. 그러나 설계자의 제한된 지식 때문에 설계 결과가 건설, 운영 및 유지보수와 같은 project lifecycle 동안 어떤 위험을 초래하는지 설계자는 인식하지 못하는 경우가 많다. 만일 설계자가 건물의 형상이 안전사고율에 미치는 영향과 이에 대응하는 안전관리비에 미치는 영향을 이해한다면, 효과적인 DFS를 진행할 수 있다. 안전관리비중 가장 많은 비중을 차지하는 낙하방지 그물망, 안전 펜스와 같은 안전시설비는 동일 건축면적이라 하더라도 건물의 형상에 따라 달라질 것이다. 본 연구의 목적은 건물의 외주길이가 안전관리비에 미치는 영향을 분석하는 것이다. 아파트(apartment building)를 대상으로 건물의 외주길이가 안전관리비에 미치는 영향을 분석한다. 본 연구를 위해 21개의 프로젝트 데이터를 수집한 후 외주길이와 건물형상계수, 건축면적을 독립변수로 안전관리비를 종속변수로 분석한다. 그 결과 외주길이와 안전관리비의 상관관계는 R²가 0.876, 형상계수와 안전관리비의 상관관계는 R²가 0.801, 건축면적과 안전관리비의 상관관계는 R²가 0.792로 매우 밀접한 관계가 있는 것으로 분석되었다.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2000년도 춘계학술대회 학술발표 논문집
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• pp.249-255
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• 2000

HRIV(Hybrid Rule-Interval Variation) method is presented to stabilize a class of nonlinear systems, where SMC(Sliding Mode Control) and ADC (ADaptive Control) schemes are incorporated to overcome the unstable characteristics of a conventional FLC(Fuzzy Logic Control). HRIV method consists of two modes: I-mode (Integral Sliding Mode PLC) and R-mode(RIV method). In I-mode, SMC is used to compensate for MAE(Minimum Approximation Error) caused by the heuristic characteristics of FLC. In R-mode, RIV method reduces interval lengths of rules as states converge to an equilibrium point, which makes the defined Lyapunov function candidate negative semi-definite without considering MAE, and the new uncertain parameters generated in R-mode are compensated by SMC. In RIV method, the overcontraction problem that the states are out of a rule-table can happen by the excessive reduction of rule intervals, which is solved with a dynamic modification of rule-intervals and a transition to I-mode. Especially, HRIV method has advantages to use the analytic upper bound of MAE and to reduce Its effect in the control input, compared with the previous researches. Finally, the proposed method is applied to stabilize a simple nonlinear system and a modified inverted pendulum system in simulation experiments.

• Mycobiology
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• 제41권4호
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• pp.214-220
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• 2013

This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost (SMC) of Pleurotus ostreatus, P. eryngii, and P. cornucopiae. Optimal enzyme recovery was achieved when SMCs were extracted with 50 mM sodium citrate (pH 4.5) buffer at $4^{\circ}C$ for 2 hr. Amylase, cellulase, and xylanase activities showed high values in extracts from P. ostreatus SMC, with 2.97 U/g, 1.67 U/g, and 91.56 U/g, respectively, whereas laccase activity and filter paper degradation ability were highest in extracts from P. eryngii SMC, with values of 9.01 U/g and 0.21 U/g, respectively. Enzymatic activities varied according to the SMCs released from different mushroom farms. The synthetic dyes remazol brilliant blue R and Congo red were decolorized completely by the SMC extract of P. eryngii within 120 min, and the decolorization ability of the extract was comparable to that of 0.3 U of commercial laccase. In addition, laccase activity of the SMC extract from P. eryngii was compared to that of commercial enzymes or its industrial application in decolorization.

• Composites Research
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• 제31권1호
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• pp.8-11
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• 2018

섬유강화 복합재료 제조 방법 중 sheet molding compound(SMC)는 자동차에 적용 가능성이 가장 높은 소재이다. 본 연구에서는 저비중 필러인 유리버블과 강화재인 유리섬유를 사용하여 물성이 저하되지 않으면서 낮은 비중을 나타내는 SMC의 제조를 위한 최적의 배합비에 대해 연구하였다. SMC 내부에 유리섬유의 혼입양이 증가할수록 강도가 향상하였고, 유리버블의 혼입양이 증가할수록 비중이 낮아졌다. 최적의 배합에서는 비중이 낮아지면서 물성이 향상되는 시너지 효과가 나타남을 발견하였다. 이러한 시너지 효과는 유리버블의 크랙 전파의 분산효과와 유리버블의 혼입에 따른 섬유와 매트릭스의 결합력의 향상에 기인한 것임을 내부구조 분석을 통해 확인하였다.

• 제어로봇시스템학회논문지
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• 제20권7호
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• pp.701-705
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• 2014

This article presents a comparison among several yaw and roll motion controllers for vehicle rollover prevention. In the previous research, yaw and roll motion controllers can be independently designed for rollover prevention. Following this idea, several yaw and roll motion controllers are designed and compared in terms of rollover prevention. For the yaw motion control, PID, LQR, SMC (Sliding Mode Control) and TDC (Time-Delay Control) are adopted. For the roll motion control, LQR, LQ SOF (Static Output Feedback) control, PID, and SMC are adopted. To compare the performance of each controller, simulation is performed on a vehicle simulation package, CarSim$^{(R)}$. From simulation, TDC and LQ SOF are the best for yaw and roll motion control, respectively.