• Title/Summary/Keyword: small samples

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Incidence of Microorganisms during Slaughtering Process of Cattle (소 도축공정 중의 미생물 증감 추이)

  • Cha Seong-Kwan;Kim Yun-Ji;Kim Myung-Ho;Shin Jeom-Ho;Lee Moo-Ha
    • Food Science of Animal Resources
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    • v.24 no.3
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    • pp.232-237
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    • 2004
  • This study was carried out to evaluate the microbiological quality of beef carcasses at different slaughtering process in large (>100 cattle/day) and small (<30 cattle/day) scale slaughtering houses. Swabbing method was used to analyze the incidence of microorganisms on brisket surface of beef carcasses in each process of after dehiding, after evisceration, before and final wash, and in cold room. In winter time, large scale slaughterhouse showed lower incidence of aerobic microorganisms (10$\^$0/∼10$^2$ CFU/$\textrm{cm}^2$) than those of small scale slaughterhouse (10$\^$0/-10$^3$ CFU/$\textrm{cm}^2$) during the slaughtering process of after dehiding, evisceration and before final wash. But samples from carcasses after final wash and in cold room storage showed no difference in aerobic cell counts between large and small scale slaughterhouse. In spring time, samples showed higher incidence of microorganisms by the log scale 1 than those of winter time in both of small and large scale slaughterhouse. After final wash, different sampling place in carcass such as rump, flank, brisket showed the different washing effect in both of small and large scale slaughterhouse. After final wash, samples from rump showed lower aerobic cell counts, but samples from flank and brisket showed higher aerobic cell counts than samples from each site before final wash.

A Survey on Adulteration and Safety of Sesame oil Circulated in Gwangju (광주지역에서 유통되고 있는 참기름의 진위판별 및 안전성 조사)

  • Yang, Yong-Shik;Kim, Jong-Pil;Seo, Kye-Won;Cho, Bae-Sick;Gang, Gyung-Lee;Kim, Eun-Sun;Park, Jong-Tae
    • Journal of Food Hygiene and Safety
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    • v.23 no.3
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    • pp.212-217
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    • 2008
  • This survey was conducted to monitor the adulteration of sesame oil circulated in Gwangju, 2007. A total of 60 samples was tested by analysis of fatty acid composition. Of these samples, 22 were from large-scale manufacturer, 25 were from small-scale manufacturer and 13 from Bangagan. First of all, in goods manufactured by large-scale company, there are no sesame oils where linolenic acid($C_{18:3}$) exceed regulatory guidance(0.5%). 5 samples from small-scale manufacturer contained linolenic acid from 0.90% to 8.38%, which means that they have other cooking oil, such as com oil, soybean oil and rape seed oil. Among Bangagan goods, only one sample have 1.20% of linolenic acid. On the other hand erucic acid($C_{22:1}$) was not detected in 60 samples at all, which means that they were not adulterated with rape seed oil. And among 6 samples of exceeding 0.5% of linolenic acid and 12 samples from Bangagan, 13 of them had benzo(a)pyrene from $0.2{\mu}g/kg\;to\;0.7{\mu}g/kg$ and the other 5 samples did not.

Microstructure and Mechanical Properties of Continuous Cast Ductile Iron (연속주조한 구상흑연주철의 미세조직과 기계적 성질)

  • Choe, Kyeong-Hwan;Cho, Gue-Serb;Lee, Kyong-Whoan;Kim, Ki-Yeong
    • Journal of Korea Foundry Society
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    • v.24 no.1
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    • pp.52-59
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    • 2004
  • Microstructure and mechanical properties of ductile cast iron were investigated in terms of diameter change of samples that gives rise to modify the microstructure due to different cooling rate in the continuous casting process. The chemical composition used in this study was GCD 400 grade. From the microstructural observation, we have found a large number of graphite with small size in diameter which is comparable to the microstructure of the sample produced by conventional sand casting. The major reason of this would he due to high cooling rate. In the sample with 26 mm in diameter, the microstructure was composed of pearlite, iron carbide, and graphite. In the samples with 60 and 100 mm in diameter, however, we have observed a dissimilar microstructure that consisting of ferrite and graphite. Concerning the mechanical property, the sample with 26 mm in diameter showed higher hardness and strength compared to those samples with 60 and 100 mm in diameter. The result obtained for ductility appeared a reversal. Much more works such as inoculation, process design and chemical composition would be required in order to have a sound product even in a small diameter of samples.

Implementation of Quad Variable Rates ADPCM Speech CODEC on C6000 DSP considering the Environmental Noise (배경잡음을 고려한 4배 가변 압축률을 갖는 ADPCM의 C6000 DSP 실시간 구현)

  • Kim Dae-Sung;Han Kyong-ho
    • Proceedings of the KIPE Conference
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    • 2002.07a
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    • pp.727-729
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    • 2002
  • In this paper, we proposed quad variable rates ADPCM coding method and its implementation on C6000 DSP, which is modified from the standard ADPCM of ITU G.726 for speech quality improvement considering the environmental noise Four coding rates, 16Kbps, 24Kbps, 32Kbps and 40Kbps are used for speech window samples and the rate decision threshold is decided by the environmental noise level. The object of the proposed method is to reduce the coding rate while retaining the speech quality and the speech quality is considerably close to 40Kbps single rate coder with the coding rate close to 16Kbps single rate coder under the environmental noise. The environmental noise level affects the coding rate and the noise level is calculated per every speech window samples. At high noise level, more samples are coded at higher rates to enhance the quality, but at low noise level, only the big speech signals are coded at higher rates and more speech samples are coded at lower coding rates to reduce the coding rates. The influence of the noise on tile speech signal is considerably high for small signals and the small signal has the higher ZCR (zero crossing rate). The method is simulated in PC and to be implemented on C6000 floating point DSP board in real time operations.

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Studies on Solvent Extraction and Flotation Technique Using Metal-Dithizone Complexes(II). Determination of Trace Elements in Water Samples by Solvent Sublation

  • 김영상;최윤석;최희선
    • Bulletin of the Korean Chemical Society
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    • v.19 no.10
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    • pp.1036-1042
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    • 1998
  • The preconcentration and determination of trace elements in water samples were studied by a solvent sublation utilizing dithizonate complexation. After metal dithizonates were formed, trace amounts of cadmium, cobalt, copper and lead were floated and extracted into small volume of a water-immiscible organic solvent on the surface of sample solution and determined in the solvent directly by GF-AAS. Several experimental conditions as formation condition of metal-dithizonate complexes, pH of solution, amount of dithizone, stirring time, the type and amount of surfactants, N2 bubbling rate and so on were optimized for the complete formation and effective flotation of the complexes. And also four kinds of light solvents were compared each other to extract the floated complexes, effectively. After the pH was adjusted to 4.0 with 5 M HNO3, 8.0 mL of 0.05% acetone solution of dithizone was added to 1.00 L water sample. The dithizonate complexes were flotated and extracted into the upper methyl isobutylketone (MIBK) layer by the addition of 2.0 mL 0.2% ethanolic sodium lauryl sulfate solution and with the aid of small nitrogen gas bubbles. And this solvent sublation method was applied to the analysis of real water samples and good results of more than 85% recoveries were obtained in spiked samples.

Preparation of Rubber Particulates for Micro Dust Study using Cryogenic Crushing

  • Chae, Eunji;Son, Chae Eun;Choi, Sung-Seen
    • Elastomers and Composites
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    • v.54 no.4
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    • pp.330-334
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    • 2019
  • Cryogenic crushing techniques have been employed for recycling waste rubber articles and for extracting residual organic additives present in rubber samples. Rubber particulate derived from tire tread abrasion is one of the key components of road dust. Therefore, in this work, we prepared rubber particulates using a cryogenic crusher and characterized their shapes as well as size distributions according to the type of rubber. The rubber particulates exhibited uneven surfaces with the presence of some small pieces. The order of the particle size distribution was observed to be: NR > BR > SBR. Subsequently, carbon black was added; this led to a decrease in the particle size and the shape becoming rougher. The crushed particulates of the carbon black-filled samples comprised agglomerated shapes of small pieces, which were similar in shape to that of wear debris in tire tread. It was discovered that crosslink density was one of the principal factors that led to the formation of small crushed particulates. The small particulates obtained by cryogenic crushing can be utilized as model rubber particulates for researching micro dust.

Fabrication, Magnetic and Magnetoresistive Properties of Bi-Doped Lanthanum Manganites (Bi 첨가 란탄 망가나이트의 제조, 자기 및 자기저항 특성)

  • 김덕실;조재경
    • Journal of the Korean Magnetics Society
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    • v.9 no.5
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    • pp.239-244
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    • 1999
  • Bi-doped lanthanum magnetics $(La_{0.67-x}Bi_xCa_{0.33}MnO_3(x\;=\;0,\; 0.04,\; 0.1,\; 0.2))$ samples have been prepared by standard ceramic process. The crystallinity and microstructures of the samples have been investigated by x-ray diffractometry and optical microscopy, respectively. The magnetic and magnetoresistive properties of the samples have been measured by vibrating sample magnetometery and van der Pauw method, respectively, at the temperatures ranging of 100 K~300 K with applied magnetic field of 0.4~0.5 T. Good crystallinity and high Curie temperature (275 K) have been obtained for the Bi-doped samples with small dosage (x = 0.04, 0.1) even they were sintered at 120$0^{\circ}C$, which is about 20$0^{\circ}C$ lower than normal sintering temperature of 140$0^{\circ}C$. The Bi-doped samples with the small dosage showed lower relative electrical resistivity and higher magneto-resistive ratio compared to the undoped sample in the most temperatures measured. The Bi-doped samples also exhibited large magnetoresisitve ratio (maximum of 15% for x = 0.1) at room temperature even under a weak magnetic field of 0.4 T.

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A Nonparametric Small Sample Estimator of Mean Residual Life

  • Farrokh Choobineh;Park, Dong-Ho
    • Journal of the Korean Statistical Society
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    • v.19 no.1
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    • pp.80-87
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    • 1990
  • In reliability and life testing the mean residual life (MRL) of an item plays a significant role. While there has been a great deal of discussion on the theoretical aspects of the MRL, good estimators of MRL have been difficult to obtain. In this paper we propose a new estimator of the MRL of items at a given age, which is especially good for a small sample. The new estimator compares favorably with the empirical MRL estimator for small samples.

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Molecular Prevalence of Cryptosporidium spp. among Companion Birds Kept in Pet Shops in Japan

  • Iijima, Yuko;Itoh, Naoyuki;Phrompraphai, Totsapon;Ito, Yoichi;Kimura, Yuya;Kameshima, Satoshi
    • Parasites, Hosts and Diseases
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    • v.56 no.3
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    • pp.281-285
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    • 2018
  • Cryptosporidium is the most common protozoan that can infect a wide range of animals, including mammals and birds. Avian Cryptosporidium spp. can cause enteric and respiratory diseases which can be fatal in birds and some species are zoonotic. Companion birds have the potential as reservoir due to their close contact with humans. Pet shops are the major source of companion birds. However, few reports are available regarding Cryptosporidium spp. infection among companion birds kept in pet shops. The present study reports the prevalence and molecular characteristics of Cryptosporidium spp. among companion birds kept in pet shops in Japan. A total of 265 fresh fecal samples were obtained from birds kept in 4 pet shops; these birds belonged to 41 species in 3 bird orders. A nested polymerase chain reaction (PCR) assay targeting the small subunit rRNA gene was employed for the detection of Cryptosporidium spp. A total of 24 samples (9.1%) were positive, and Cryptosporidium spp. were detected from all pet shops. The prevalence of Cryptosporidium spp. in each of the bird orders was 6.5% (10/153) in Psittaciformes, 14.4% (13/90) in Passeriformes, and 4.5% (1/22) in Galliformes. Based on sequence analysis, 13 (54.2%) isolates were classified to C. galli, 8 (33.3%) were avian genotype III, and the remaining 3 (12.5%) were C. baileyi. No infection with zoonotic C. meleagridis and no coinfection with multiple Cryptosporidium spp. and/or genotypes were observed. The zoonotic potential of Cryptosporidium spp. infecting companion birds kept in pet shops in Japan is likely to be low.

Change in the Microbial Profiles of Commercial Kimchi during Fermentation (국내 시판김치의 김치담금부터 숙성까지의 미생물 균총 변화)

  • Chang, Ji-Yoon;Choi, Yu-Ri;Chang, Hae-Choon
    • Food Science and Preservation
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    • v.18 no.5
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    • pp.786-794
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    • 2011
  • To investigate the sanitary-quality level of commercial kimchi in South Korea, the pH, acidity, and microbial-flora changes in the kimchi were determined. Samples of kimchi produced by three different manufacturers (a small grocery store, a small/medium-sized enterprise, and a large food company) were collected. Freshly made kimchi was purchased and fermented at $10^{\circ}C$ for 10 days. The pH of the commercial kimchi on the purchased day was approximately pH 5.8, and that on the $10^{th}$ day of fermentation was ${\simeq}pH$ 4.1. The kimchi purchased from a large company showed a more rapid decline in pH level during fermentation. The saltiness of the kimchi purchased from a medium-sized company was slightly higher than those of the other commercial kimchi samples. The saccharinity index of the kimchi produced by a small grocery store was higher than those of the other samples, and its value deviation was also higher than those of the other commercial kimchi samples. A higher total viable-cell count and a higher lactic-acid bacteria (LAB) count were detected in the kimchi from the large food company at the beginning of fermentation compared to the samples of the two other kimchi manufacturers. The highest cell numbers of gram-positive bacteria (except LAB) and coliform bacteria were detected from the small-grocery-store kimchi, but the coliform bacteria count gradually decreased during fermentation although such bacteria were still detected until the $10^{th}$ day of fermentation. In contrast, coliform bacteria were not detected in the samples from the medium-sized and large food companies. Yeast, which is detected in over-ripened kimchi, was detected in the unfermented kimchi from the small grocery store, which had a below-0.36% acidity level. The gram-positive bacteria (except LAB) that were detected in all the tested commercial kimchi samples were determined to be Bacillus spp., and the gram-negative bacteria were determined to be Escherichia coli, Enterobacter spp., Sphingomonase spp., and Strenophomonas spp. The proportions of all the aforementioned bacteria in the kimchi samples, however, were different depending on the samples that were taken. These results indicate that a more sanitary kimchi production process and a more systematic kimchi production manual should be developed to industrialize and globalize kimchi.