• 한국농공학회지
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• 제17권3호
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• pp.3878-3884
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• 1975

This study was carried out to develop the throw-in type thresher with its size as small as possible. Developing the smallest possible size of the throw-in type thresher has been very important to increase mobility and to reduce the machine price. The thresher that developed for this purpose was tested as to threshing and separation performance for the samples collected in eight catch boxes under the concave while threshing. The amount of grain collected in each compartments was measured and the threshing and separating pattern along the total span of the threshing drum was determined. The performance of separating and threshing units of the test thresher and threshing loss was evaluated by use of the developed grain separating apparatus and the method for measuring the grain separating performance of threshers. The results are summarized as follows; 1. The unthreshed grain (drum losses) and semi-threshed grain did not appeared at all throughout the treatments. 2. When threshed by making use of the developed throw-in type thresher, the threshing grain loss at about 25 per cent grain moisture was about one-half when threshed at about 18 per cent grain moisture. 3. And its grain separating loss in higher feed rate was decreased in comparison with that of lower feed rate. These results suggests that the throw-in type thresher may be suitable for wet threshing and for higher feed rate of threshing. 4. Above 60 per cent of total grain passing through concave fell through the screen within a scant 30 cm from the feeding inlet. This threshing pattern may suggest that major threshing action may be finished before about one third of cylinder length. The required separating load extended over the whole drum span is so defferent that separating elements should be redesigned so as to accomodate this variable pattern of separation load. 5. It was apparent from the experiment that the length of the threshing drum of the throw-in type thresher could be reduced from 1285mm to about 1050mm without increasing grain separation loss greatly.

• The Korean Journal of Physiology
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• 제8권1호
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• pp.55-65
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• 1974

The effect of ginseng on the ATPase activity of rabbit ref cell membrane has been investigated. The experiments were also designed to determine whether the components of ginseng could be attributed to the effect on ATPase activity which dependent upon sodium plus potassium and is sensitive to ouabain. The following results were observed. 1. The activity of the $Na^{+}-K^{+}-ATPase$ from red cell membrane is stimulated by ginseng, and the concentration of ginseng for half-maximal activity is about 15 mg%. The pH optimum for the ginseng sensitive component is 7.6. 2. The portion of the enzyme activity stimulated by ginseng is completely abolished by ouabain. 3. The activating effect of ginseng on the ATPase, with a given concentration of sodium in the medium, is increased by raising the potassium concentration but activity ratio is decreased. 4. The activating effect of ginseng on the ATPase, with a given concentration of potassium in the medium, is increased by raising the sodium concentration but the activity ratio is decreased. 5. The ATPase activity is increased by small amounts of calcium but inhibited by larger amounts and the rate of activity by ginseng is constant. 6. The action of ginseng on the ATPase activity was not related to the sulfhydryl group of cysteine, the amino group of lysine, the imidazole group of histidine, the quanidinium group of arginine, the carboxyl group of aspartic acid, or the hydroxyl group of threonine. 7. The activating effect of ginseng on the ATPase activity may be not due to a saponin which is contained in ginseng.

• 한국미생물·생명공학회지
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• 제14권5호
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• pp.407-413
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• 1986

분쇄마찰매체에 의한 무증자 생전분의 효소당화촉진 mechanism을 전분의 구조적 측면을 중심으로 규명하였다. 당화촉진 효과를 줄 수 있는 수준의 분쇄마찰매체의 기계적 교반운동은 생전분의 미세결정구조(microcrystalline structure) 파괴는 물론 전분입자를 붕괴 (fragmentation) 시키는 효과도 없었다. 생전분 입자구조 변화의 중요한 특징은 입자구조의 팽윤(swelling) 현상으로써 팽윤된 전분은 보수능력이 2.5배 정도까지 증가되었다. 이와 같은 기계적 충격에 의한 전분입자의 팽윤현상은 가열호화에 의한 $\alpha$-전분화에 따른 팽윤현상가는 상이하였다. 생전분을 장시간 bead-milling하여 전처리하며 팽윤시킨 생전분은 당화가 촉진되었으나 bead와 효소를 동시에 첨가시킨 경우의 당화속도와 수율에는 미치지 못하였다. 분쇄마찰 반응계에서 효소를 첨가 무증자 전분을 당화시킬 경우에는 생전분입자 2시간 전후하여 수많은 입자로 fragmentation되었다. 생전분의 당화촉진 mechanism은 분쇄마찰매체에 의하여 전분입자가 균열팽윤되고 이 팽윤된 생전분은 보다 쉽게 효소작용을 받아 침식되며 이 침식된 전분입자는 분쇄마찰매체에 의하여 더욱 가속적으로 fragmentation되어 효소작용이 촉진된다고 판단된다. 옥수수, 감자, 고구마 등 각종 생전분은 그 종류에 따라 분쇄마찰 반응계를 활용한 무증자 당화에 많은 차이가 있었으며 이를 전분입자의 구조와 연결시켜 고찰하였다.

• 한국미생물·생명공학회지
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• 제20권5호
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• pp.511-518
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• 1992

Serratia marcescens ATCC 27117에서 부터 클로닝한 58KD 키티나아제 유전자를 subcloning 하여 2.6Kb DNA 삽입단편을 가진 플라스미드 pCHI26을 제조하고 대장균에서 발현과 분비를 살펴보았다. 키티니아제 유전자는 대장균에서 자신의 prmoter를 이용하여 매우 낮은 수준(<5mU/m$\ell$)으로 발현되었으며 lac promoter를 이용하는 경우 키티니아제 발현이 증가되어 약 80mU/m$\ell$가 되었다. 발현된 키티니아제는 거의 전적으로 대장균의 periplasm에 위치(약 87.8)하고 있었다. 배양시간에 따라서 세포내 키티니아제 활성을 측정해본 결과 초기정지기까지는 균체 성장과 비례해서 세포내 효소활성이 증가되었으나 정지기부터 세포내 효소활성이 급격히 줄어드는 양상을 보였다. 그러나 이 기간 동안 세포의 효소활성의 변화는 거의 없었다. 이러한 결과로 보아 periplasm에 위치한 키티나아제가 대장균의 단백분해효소에 의해서 분해되는 것으로 추정되었다.

• 보존과학연구
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• 통권34호
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• pp.84-99
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• 2013

전 세계적으로 분포하고 있는 약 2,900 종의 흰개미 중 23종의 흰개미가 일본에 서식하고 있으며, 이에 따라 1종의 흰개미만 분포하는 우리나라에 비해 더 심각한 피해를 주는 것으로 알려져 있다. 일본에서는 목조건축물에서 흰개미에 의한 피해가 발생하면 화학적 방제(훈증처리, 토양처리, 베이트처리 등)나 비화학적 방제방법(저산소처리, 이산화탄소처리, 고온처리, 저온처리 등)을 이용해 방제처리를 하게 된다. 특히, 훈증처리에 사용되는 메틸 브로마이드 훈증제의 생산과 소비를 2005년부터 전폐하기로 함에 따라 이를 대체하기 위한 다양한 방법들에 대한 검토가 이루어졌으며, 그 결과 실제로 피해 정도가 소규모이거나 장기간의 처리가 가능한 경우 등 일부 경우에는 약제를 사용하지 않는 비화학적 방법이 효과적이라는 것이 확인되었다.

• International Journal of Oral Biology
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• 제45권3호
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• pp.115-125
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• 2020

Resveratrol has been reported to exert anticancer activity via modulation of multiple pathways and genes. In this study, we examined the effect of resveratrol on YD-10B human oral squamous cell carcinoma cells and its molecular mechanisms of action. We found that resveratrol inhibited the proliferation of YD-10B cells in a dose- and time-dependent manner. The suppressive effect of resveratrol was accompanied by a reduction in Bmi-1 gene expression. We observed that silencing the Bmi-1 gene by small interfering RNA effectively downregulated the levels of GLUT1 mRNA and protein, which were also repressed by resveratrol. Bmi-1 silencing increased the number of YD-10B cells in S-phase arrest by approximately 2.3-fold compared with the control. In conclusion, the results of the present study demonstrate, for the first time, that resveratrol suppresses Bmi-1-mediated GLUT1 expression in human oral squamous cell carcinoma cells and suggest that the specific molecular targeting of Bmi-1 and/or GLUT1 expression can be combined with a chemotherapeutic strategy to improve the response of oral cancer cells to resveratrol.

• Animal cells and systems
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• 제11권2호
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• pp.129-134
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• 2007

The initiation of eukaryotic DNA replication requires assembly of the pre-replicative complex (Pre-RC) through the concerted action of Orc, Cdc6, Cdt1 and Mcm2-7 complex during G1 phase. The pre-RC assembly licenses individual replication origins for the initiation of DNA replication and sufficient number of the pre-RC is essential for proper progression of S phase. However, it is not well known how cells recognize the completion of the pre-RC assembly before G1-S transition. In order to understand the cellular responses to the defects in pre-RC assembly, we depleted the known components of pre-RC proteins using the small interference RNAs in HeLa cells. Although the defects of pre-RC assembly by the depletion of the pre-RC proteins such as Orc2, Cdt1, Mcm2 & Mcm10 did not elicit the activation of Chk1- or Chk2-dependent checkpoint pathways, these cells still showed significant decrease in the cellular level of Cdc25A proteins. These results suggests that a novel checkpoint pathway exist in HeLa cells, which is not dependent upon Chk1 or Chk2 proteins and play essential roles in the cellular responses to the defects in the pre-RC assembly. Also, among those four proteins tested in this study, the depletion of Mcm10 and Cdt1 proteins significantly increased the apoptotic cell death in HeLa cells, suggesting that these proteins not only play roles in the pre-RC assembly, but also are involved in the checkpoint responses to the defects in the pre-RC assembly.

• Structural Engineering and Mechanics
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• 제20권5호
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• pp.505-526
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• 2005

The influence of the inter-story structural pounding on the seismic behaviour of adjacent multistory reinforced concrete structures with unequal total heights and different story heights is investigated. Although inter-story pounding is a common case in practice, it has not been studied before in the literature as far as the authors are aware. Fifty two pounding cases, each one for two different seismic excitations, are examined. From the results it can be deduced that: (i) The most important issue in the inter-story pounding is the local effect on the external column of the tall building that suffers the impact from the upper floor slab of the adjacent shorter structure. (ii) The ductility demands for this column are increased comparing with the ones without the pounding effect. In the cases that the two buildings are in contact these demands appear to be critical since they are higher than the available ductility values. In the cases that there is a small distance between the interacting buildings the ductility demands of this column are also higher than the ones of the same column without the pounding effect but they appear to be lower than the available ductility values. (iii) It has to be stressed that in all the examined cases the developed shear forces of this column exceeded the shear strength. Thus, it can be concluded that in inter-story pounding cases the column that suffers the impact is always in a critical condition due to shear action and, furthermore, in the cases that the two structures are in contact from the beginning this column appears to be critical due to high ductility demands as well. The consequences of the impact can be very severe for the integrity of the column and may be a primary cause for the initiation of the collapse of the structure. This means that special measures have to be taken in the design process first for the critically increased shear demands and secondly for the high ductility demands.

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9211-9216
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• 2014

CXCR7 is involved in tumor development and metastasis in multiple malignancies. However, the function and molecular mechanisms of action of CXCR7 in human cervical cancer are still unclear. In the present study a loss of-function approach was used to observe the effects of recombinant CXCR7 specific small interfering RNA pBSilence1.1 plasmids on biological behavior including proliferative activity and invasive potential, as indicated by MTT assays with the cervical cancer SiHa cell line in vitro. Reverse transcription polymerase chain reaction and Western blotting revealed that CXCR7 was downregulated in transfected compared with control cells, associated with inhibited cell growth, invasiveness and migration. The expression of CXCR7 and CXCL12 was also determined immunohistochemically in 152 paraffin-embedded, cervical squamous cell carcinoma (CSCC) and cervical intraepithelial neoplasia (CIN), or normal cervical epithelial to assess clinico-pathological pattern and CXCR7 status with respect to cell differentiation and lymph node metastasis in Uighur patients with CSCC. CXCR7 and CXCL12 expression was higher in cervical cancer than CIN and normal cervical mucosa, especially in those with higher stage and lymph node metastasis. CXCL12 appeared to be positively regulated by CXCR7 at the post-transcriptional level in CSCC. We propose that aberrant expression of CXCR7 plays a role in carcinogenesis, differentiation and metastasis of CSCC, implying its use as a potential target for clinical biomarkers in differentiation and lymph node metastasis.

• 한국과학교육학회지
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• 제1권1호
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• pp.73-88
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• 1978

The survey is intended to improve science education by the analysis of problems produced in the following nine aspects (objectives, contents, materials, inquring methods, teaching methods, teachers, children, evalutions, admininative supports) during the past five years often the revision of new science curriculum. 1. View for science education. 1) The teacher's and children's view for science education can be seen renewed, being different from the old one. 2) Inquring method in learning and teaching began to take firm root and children came to know that it is the only way to study science. 3) Writers think that the new science education has stepped into the stage of being fixed to a considerable degree. 2. The aspect of administrative policy. 1) The amount of materials in possession is small and the present materials are lacking in solidity and precision. 2) Class room teacher's over load with miscellaneous things is a difficult problem to solve. 3) The shortage of the printed materials and books for children and teachers has an influence on the development of inquring method. 4) It causes cramming to examine all children at the same time by paper test. 5) It is more desirable to appoint qualified teacher only for science teaching. 3. Contents of science curriculum. 1) In current science textbooks. There can be found some contents which are difficult for teachers to understand and not in accordance with the reality of Korea. Therefore, it is imperative that contents of science textbooks should be reexamined. 2) As it is hard to teach concepts of reciprocal action and to prepare materials with teaching of the system of biological concepts, the teaching of contents is likely to be a cramming. 4. The aspect of in-service education. 1) It is obsolutely predominant reation that in-service education is making a great contribution to class room teaching. 2) As adiministrators' policy for science education has a great effect, in-service education for their enlightenment is needed.