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http://dx.doi.org/10.11620/IJOB.2020.45.3.115

Resveratrol inhibits cell growth via targeting the Bmi-1 pathway in YD-10B human oral squamous cell carcinoma cells  

Park, Kyoung-Eun (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
Ok, Chang Youp (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
Jang, Hye-Ock (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
Bae, Moon-Kyoung (Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University)
Bae, Soo-Kyung (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
Publication Information
International Journal of Oral Biology / v.45, no.3, 2020 , pp. 115-125 More about this Journal
Abstract
Resveratrol has been reported to exert anticancer activity via modulation of multiple pathways and genes. In this study, we examined the effect of resveratrol on YD-10B human oral squamous cell carcinoma cells and its molecular mechanisms of action. We found that resveratrol inhibited the proliferation of YD-10B cells in a dose- and time-dependent manner. The suppressive effect of resveratrol was accompanied by a reduction in Bmi-1 gene expression. We observed that silencing the Bmi-1 gene by small interfering RNA effectively downregulated the levels of GLUT1 mRNA and protein, which were also repressed by resveratrol. Bmi-1 silencing increased the number of YD-10B cells in S-phase arrest by approximately 2.3-fold compared with the control. In conclusion, the results of the present study demonstrate, for the first time, that resveratrol suppresses Bmi-1-mediated GLUT1 expression in human oral squamous cell carcinoma cells and suggest that the specific molecular targeting of Bmi-1 and/or GLUT1 expression can be combined with a chemotherapeutic strategy to improve the response of oral cancer cells to resveratrol.
Keywords
Resveratrol; Bmi-1; GLUT1; Human oral squamous cell carcinoma;
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